Massilia armeniaca
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5145 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2R4C4G3|A0A2R4C4G3_9BURK Helicase OS=Massilia armeniaca OX=2072590 GN=C9I28_00985 PE=4 SV=1
MM1 pKa = 7.43 KK2 pKa = 10.55 NIFTFRR8 pKa = 11.84 AASIAAQEE16 pKa = 4.52 ADD18 pKa = 3.21 DD19 pKa = 4.37 SYY21 pKa = 11.85 IVGFADD27 pKa = 4.86 SRR29 pKa = 11.84 SQPQEE34 pKa = 3.63 YY35 pKa = 9.93 IILQRR40 pKa = 11.84 SVYY43 pKa = 10.08 PDD45 pKa = 3.79 GEE47 pKa = 4.29 PDD49 pKa = 3.33 EE50 pKa = 5.97 DD51 pKa = 4.24 EE52 pKa = 4.86 PDD54 pKa = 3.53 TYY56 pKa = 11.08 FLEE59 pKa = 5.26 INDD62 pKa = 4.05 QATSGYY68 pKa = 10.92 GGIQSATLSQDD79 pKa = 2.71 SFTLVLADD87 pKa = 4.3 DD88 pKa = 4.49 NEE90 pKa = 4.39 YY91 pKa = 11.27 SDD93 pKa = 4.92 GLTAVTVRR101 pKa = 11.84 FDD103 pKa = 3.77 AAQLPADD110 pKa = 4.43 LKK112 pKa = 10.98 QYY114 pKa = 10.41 LGQIFHH120 pKa = 6.63 GTDD123 pKa = 3.26 CTFTASS129 pKa = 3.21
Molecular weight: 14.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.706
IPC2_protein 3.706
IPC_protein 3.694
Toseland 3.478
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.668
Rodwell 3.528
Grimsley 3.389
Solomon 3.681
Lehninger 3.643
Nozaki 3.821
DTASelect 4.075
Thurlkill 3.541
EMBOSS 3.668
Sillero 3.821
Patrickios 0.896
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.746
Protein with the highest isoelectric point:
>tr|A0A2R4CIF2|A0A2R4CIF2_9BURK IS1595 family transposase OS=Massilia armeniaca OX=2072590 GN=C9I28_21240 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 9.97 QPSVVRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.57 RR14 pKa = 11.84 THH16 pKa = 5.79 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATRR25 pKa = 11.84 GGRR28 pKa = 11.84 QVLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.65 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5145
0
5145
1777161
27
3246
345.4
37.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.261 ± 0.055
0.884 ± 0.012
5.482 ± 0.025
5.06 ± 0.031
3.41 ± 0.019
8.353 ± 0.035
2.228 ± 0.017
4.329 ± 0.026
3.271 ± 0.029
10.549 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.318 ± 0.016
2.894 ± 0.027
5.185 ± 0.028
3.968 ± 0.023
6.861 ± 0.033
5.048 ± 0.028
5.505 ± 0.04
7.458 ± 0.028
1.39 ± 0.016
2.545 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here