Anaeroplasma bactoclasticum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Anaeroplasmatales; Anaeroplasmataceae; Anaeroplasma

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1953 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A397RP69|A0A397RP69_9MOLU G domain-containing protein OS=Anaeroplasma bactoclasticum OX=2088 GN=EI71_00954 PE=4 SV=1
MM1 pKa = 7.72ILDD4 pKa = 4.33GDD6 pKa = 3.59ILAYY10 pKa = 10.35FNEE13 pKa = 4.1IGVDD17 pKa = 3.78VEE19 pKa = 4.11EE20 pKa = 4.85SSYY23 pKa = 11.69EE24 pKa = 3.92DD25 pKa = 3.15TFSFIFQISYY35 pKa = 11.38SDD37 pKa = 3.72FDD39 pKa = 4.65LNATFTIDD47 pKa = 3.57RR48 pKa = 11.84SLNLLSFMVSLPNPEE63 pKa = 4.46EE64 pKa = 4.08FDD66 pKa = 4.32LLPEE70 pKa = 4.36ALNAINSNSTLLKK83 pKa = 10.34AYY85 pKa = 10.1YY86 pKa = 10.06DD87 pKa = 3.82KK88 pKa = 11.52LDD90 pKa = 3.53DD91 pKa = 3.71TVYY94 pKa = 10.88VKK96 pKa = 11.05ASTFVTEE103 pKa = 4.69DD104 pKa = 2.98NCIQAMDD111 pKa = 5.12FILTSMKK118 pKa = 10.81DD119 pKa = 3.05LDD121 pKa = 3.69YY122 pKa = 11.18EE123 pKa = 4.45YY124 pKa = 10.66IEE126 pKa = 4.24NLYY129 pKa = 10.88NSIYY133 pKa = 10.63KK134 pKa = 10.01EE135 pKa = 4.6DD136 pKa = 3.95YY137 pKa = 11.0DD138 pKa = 4.8SFDD141 pKa = 4.49DD142 pKa = 4.44EE143 pKa = 4.81FEE145 pKa = 4.31KK146 pKa = 10.91AHH148 pKa = 7.02KK149 pKa = 8.7DD150 pKa = 3.34TSIEE154 pKa = 3.73EE155 pKa = 4.1DD156 pKa = 3.16MAEE159 pKa = 5.0LMEE162 pKa = 4.8LLKK165 pKa = 11.13NKK167 pKa = 9.5NRR169 pKa = 3.91

Molecular weight: 19.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A397S0A5|A0A397S0A5_9MOLU Uncharacterized protein OS=Anaeroplasma bactoclasticum OX=2088 GN=EI71_00801 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.68RR10 pKa = 11.84KK11 pKa = 10.17RR12 pKa = 11.84KK13 pKa = 6.65VTHH16 pKa = 5.96GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.27NGRR28 pKa = 11.84KK29 pKa = 9.0ILAARR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.65KK37 pKa = 10.42GRR39 pKa = 11.84KK40 pKa = 8.09QLTVV44 pKa = 2.97

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1953

0

1953

661975

39

4180

339.0

38.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.836 ± 0.06

1.263 ± 0.021

6.258 ± 0.041

7.196 ± 0.063

4.662 ± 0.045

5.914 ± 0.056

1.61 ± 0.023

8.74 ± 0.067

8.494 ± 0.07

9.107 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.553 ± 0.029

6.009 ± 0.056

2.8 ± 0.029

2.187 ± 0.024

3.232 ± 0.035

6.426 ± 0.063

5.322 ± 0.072

6.215 ± 0.053

0.741 ± 0.018

5.436 ± 0.059
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski