Vagococcus silagei

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Vagococcus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2379 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S3B009|A0A4S3B009_9ENTE ABC transporter ATP-binding protein OS=Vagococcus silagei OX=2508885 GN=ESZ54_10855 PE=4 SV=1
MM1 pKa = 7.16TLAKK5 pKa = 9.5IVYY8 pKa = 10.15ASLTGNTEE16 pKa = 3.84EE17 pKa = 4.48IADD20 pKa = 3.97VVAEE24 pKa = 4.09ALEE27 pKa = 4.29EE28 pKa = 3.78QDD30 pKa = 2.77ITVEE34 pKa = 4.2INEE37 pKa = 4.41CTQVDD42 pKa = 3.39AMDD45 pKa = 4.24FEE47 pKa = 4.6EE48 pKa = 6.3ADD50 pKa = 3.22ICIVATYY57 pKa = 9.29TYY59 pKa = 11.13DD60 pKa = 4.52DD61 pKa = 4.43GNLPDD66 pKa = 5.26EE67 pKa = 5.05IMDD70 pKa = 4.75FYY72 pKa = 11.79DD73 pKa = 4.37EE74 pKa = 5.24LLEE77 pKa = 5.79LDD79 pKa = 4.4LTGKK83 pKa = 8.99TYY85 pKa = 10.98GVVGSGDD92 pKa = 3.48SFYY95 pKa = 11.33DD96 pKa = 3.87LYY98 pKa = 11.2CIAVDD103 pKa = 4.11KK104 pKa = 10.82FDD106 pKa = 3.82EE107 pKa = 4.57AFAKK111 pKa = 10.41IGATKK116 pKa = 10.17GAEE119 pKa = 3.93NVKK122 pKa = 10.19IEE124 pKa = 4.24LACEE128 pKa = 3.53EE129 pKa = 4.34DD130 pKa = 5.15DD131 pKa = 3.97IVKK134 pKa = 10.72LEE136 pKa = 3.97EE137 pKa = 3.79FAKK140 pKa = 10.6KK141 pKa = 10.23VVEE144 pKa = 4.15KK145 pKa = 10.96ALL147 pKa = 3.69

Molecular weight: 16.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S3B890|A0A4S3B890_9ENTE CoA-disulfide reductase OS=Vagococcus silagei OX=2508885 GN=ESZ54_07005 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.7QPKK8 pKa = 8.83KK9 pKa = 9.05RR10 pKa = 11.84KK11 pKa = 7.43RR12 pKa = 11.84QKK14 pKa = 8.87VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.8GRR39 pKa = 11.84KK40 pKa = 8.76VLAAA44 pKa = 4.31

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2379

0

2379

727940

26

3222

306.0

34.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.506 ± 0.055

0.633 ± 0.014

5.493 ± 0.041

7.442 ± 0.066

4.628 ± 0.045

6.201 ± 0.052

1.794 ± 0.023

7.921 ± 0.051

7.458 ± 0.055

9.918 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.706 ± 0.027

4.911 ± 0.038

3.284 ± 0.026

4.13 ± 0.043

3.562 ± 0.036

6.139 ± 0.039

5.941 ± 0.04

6.886 ± 0.041

0.822 ± 0.016

3.622 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski