Bacteroidaceae bacterium
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3558 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6F9Z7S0|A0A6F9Z7S0_9BACT Outer membrane protein 41 OS=Bacteroidaceae bacterium OX=2212467 GN=IMSAGC004_00376 PE=4 SV=1
MM1 pKa = 7.48 KK2 pKa = 10.23 RR3 pKa = 11.84 NEE5 pKa = 4.11 YY6 pKa = 10.01 FKK8 pKa = 10.76 RR9 pKa = 11.84 AADD12 pKa = 4.31 LYY14 pKa = 11.33 GSGQVSGEE22 pKa = 4.28 VYY24 pKa = 10.83 DD25 pKa = 4.34 AMIMNADD32 pKa = 4.03 IFCDD36 pKa = 4.19 DD37 pKa = 4.22 EE38 pKa = 5.75 EE39 pKa = 4.65 EE40 pKa = 5.86 DD41 pKa = 3.56 EE42 pKa = 5.86 DD43 pKa = 5.43 GYY45 pKa = 11.62 QGGLPDD51 pKa = 3.83 TYY53 pKa = 11.75 AEE55 pKa = 4.09 VEE57 pKa = 4.16 YY58 pKa = 11.22 SDD60 pKa = 4.57 FEE62 pKa = 4.22 NPEE65 pKa = 4.03 AVDD68 pKa = 3.6 GARR71 pKa = 11.84 FDD73 pKa = 3.67 DD74 pKa = 4.17 MNYY77 pKa = 10.13 LRR79 pKa = 11.84 YY80 pKa = 8.61 TEE82 pKa = 4.1 RR83 pKa = 4.91
Molecular weight: 9.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.795
IPC_protein 3.745
Toseland 3.541
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.579
Grimsley 3.465
Solomon 3.719
Lehninger 3.668
Nozaki 3.859
DTASelect 4.05
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.872
Patrickios 0.896
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|A0A6F9ZBR0|A0A6F9ZBR0_9BACT Uncharacterized protein OS=Bacteroidaceae bacterium OX=2212467 GN=IMSAGC004_01462 PE=4 SV=1
MM1 pKa = 7.17 KK2 pKa = 9.79 TFPHH6 pKa = 6.28 YY7 pKa = 10.68 LQLDD11 pKa = 3.67 AMDD14 pKa = 5.55 CDD16 pKa = 4.3 PTCLCMIAKK25 pKa = 9.87 YY26 pKa = 8.76 YY27 pKa = 11.02 ARR29 pKa = 11.84 TIRR32 pKa = 11.84 FRR34 pKa = 11.84 CSAPAPSSHH43 pKa = 6.04 ARR45 pKa = 11.84 GYY47 pKa = 10.2 RR48 pKa = 11.84 CWASVTRR55 pKa = 11.84 RR56 pKa = 11.84 NPFAGVSRR64 pKa = 11.84 TGVRR68 pKa = 11.84 IHH70 pKa = 6.2 RR71 pKa = 11.84 RR72 pKa = 11.84 KK73 pKa = 10.62 GGVHH77 pKa = 6.34 FPRR80 pKa = 11.84 ARR82 pKa = 11.84 RR83 pKa = 11.84 TGKK86 pKa = 9.74 VRR88 pKa = 11.84 IGNSTAAGADD98 pKa = 3.28 HH99 pKa = 7.09 PLWQRR104 pKa = 11.84 TAFGKK109 pKa = 10.38 DD110 pKa = 2.73 HH111 pKa = 7.58 DD112 pKa = 4.76 GNGIHH117 pKa = 6.33 RR118 pKa = 11.84 HH119 pKa = 5.08 EE120 pKa = 4.62 GKK122 pKa = 9.95 KK123 pKa = 10.37 ACSAGCC129 pKa = 3.58
Molecular weight: 14.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.692
IPC_protein 10.57
Toseland 10.701
ProMoST 10.438
Dawson 10.804
Bjellqvist 10.555
Wikipedia 11.023
Rodwell 10.965
Grimsley 10.847
Solomon 10.935
Lehninger 10.906
Nozaki 10.73
DTASelect 10.54
Thurlkill 10.716
EMBOSS 11.111
Sillero 10.745
Patrickios 10.701
IPC_peptide 10.935
IPC2_peptide 9.926
IPC2.peptide.svr19 8.456
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3558
0
3558
1234531
29
3015
347.0
39.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.991 ± 0.037
1.321 ± 0.017
5.474 ± 0.03
6.653 ± 0.04
4.584 ± 0.03
6.849 ± 0.039
2.007 ± 0.018
6.849 ± 0.038
6.834 ± 0.032
9.001 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.849 ± 0.021
5.15 ± 0.036
3.695 ± 0.022
3.421 ± 0.025
4.647 ± 0.029
5.896 ± 0.027
5.497 ± 0.028
6.438 ± 0.032
1.324 ± 0.017
4.519 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here