Blackberry virus F

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Caulimoviridae; Badnavirus

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0X8TLD2|A0A0X8TLD2_9VIRU Uncharacterized protein OS=Blackberry virus F OX=2560349 PE=4 SV=1
MM1 pKa = 5.96TAEE4 pKa = 5.27RR5 pKa = 11.84IEE7 pKa = 4.07QSIKK11 pKa = 11.0DD12 pKa = 3.45WDD14 pKa = 3.98DD15 pKa = 3.21KK16 pKa = 11.87YY17 pKa = 11.35KK18 pKa = 11.28VKK20 pKa = 10.93DD21 pKa = 3.58LDD23 pKa = 3.87YY24 pKa = 11.51LNLEE28 pKa = 4.41VPQPSSFQNLSSLGFFCFHH47 pKa = 6.17QNCKK51 pKa = 10.27HH52 pKa = 5.83SLEE55 pKa = 4.6SSSSQLNLLTDD66 pKa = 4.36LALVQHH72 pKa = 6.73LNLEE76 pKa = 4.64FTHH79 pKa = 6.43SRR81 pKa = 11.84VGLSARR87 pKa = 11.84IAIKK91 pKa = 10.35YY92 pKa = 7.02FHH94 pKa = 7.15SISNQLKK101 pKa = 9.0EE102 pKa = 4.35QEE104 pKa = 4.34EE105 pKa = 4.15QLKK108 pKa = 8.08TVAATQKK115 pKa = 10.55KK116 pKa = 8.78IQKK119 pKa = 7.96QLKK122 pKa = 8.31SLEE125 pKa = 4.46GEE127 pKa = 4.43LEE129 pKa = 3.73QHH131 pKa = 6.41RR132 pKa = 11.84PLSRR136 pKa = 11.84TDD138 pKa = 3.24VQQLVQEE145 pKa = 5.02IAQQPKK151 pKa = 10.15LVEE154 pKa = 4.21AEE156 pKa = 4.2ALRR159 pKa = 11.84LTQDD163 pKa = 3.5LEE165 pKa = 4.13RR166 pKa = 11.84RR167 pKa = 11.84LNEE170 pKa = 4.04VKK172 pKa = 10.68DD173 pKa = 3.67LLGEE177 pKa = 4.17VKK179 pKa = 10.36KK180 pKa = 10.97ALLSS184 pKa = 3.7

Molecular weight: 21.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0X8R425|A0A0X8R425_9VIRU Reverse transcriptase OS=Blackberry virus F OX=2560349 PE=4 SV=1
MM1 pKa = 7.56SDD3 pKa = 2.68WRR5 pKa = 11.84VTQGSSVYY13 pKa = 10.56KK14 pKa = 10.26EE15 pKa = 3.7ATQALEE21 pKa = 5.02GLDD24 pKa = 3.66QPALGFARR32 pKa = 11.84GTDD35 pKa = 3.78FAGATNSSLQGIIKK49 pKa = 9.06QQNLQIQLLVKK60 pKa = 9.73LTEE63 pKa = 4.21KK64 pKa = 11.11VEE66 pKa = 4.63DD67 pKa = 3.72LQQEE71 pKa = 4.36IKK73 pKa = 9.3TLKK76 pKa = 9.42AAKK79 pKa = 9.72AKK81 pKa = 10.26AAAEE85 pKa = 4.1SSSEE89 pKa = 3.97DD90 pKa = 3.93LEE92 pKa = 4.32SQIIGLSKK100 pKa = 10.94KK101 pKa = 9.97LDD103 pKa = 3.63KK104 pKa = 11.22VSLGTGVVPKK114 pKa = 10.41KK115 pKa = 9.19RR116 pKa = 11.84TPYY119 pKa = 9.85YY120 pKa = 10.57VKK122 pKa = 10.24EE123 pKa = 4.31DD124 pKa = 3.45PAKK127 pKa = 9.61IFEE130 pKa = 4.41RR131 pKa = 11.84EE132 pKa = 3.83FAKK135 pKa = 10.29TKK137 pKa = 10.67

Molecular weight: 15.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2269

137

1948

756.3

86.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.082 ± 1.033

1.631 ± 0.533

5.156 ± 0.088

8.991 ± 0.233

2.953 ± 0.092

5.685 ± 1.002

2.248 ± 0.539

5.553 ± 0.603

7.977 ± 1.301

7.801 ± 2.976

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.424 ± 0.883

3.526 ± 0.52

4.848 ± 1.154

5.421 ± 1.933

5.729 ± 1.018

7.316 ± 0.701

5.862 ± 0.576

5.377 ± 0.133

1.587 ± 0.472

3.834 ± 0.963
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski