Gramella fulva

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Gramella

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3785 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R3ZB15|A0A2R3ZB15_9FLAO Probable potassium transport system protein kup OS=Gramella fulva OX=2126553 GN=trkD PE=3 SV=1
MM1 pKa = 7.33VKK3 pKa = 10.39GFLVAFQLLFFLMACSSEE21 pKa = 4.17DD22 pKa = 3.41TPGQPKK28 pKa = 8.11PTPDD32 pKa = 4.34PDD34 pKa = 3.67PVAKK38 pKa = 10.09ADD40 pKa = 3.67KK41 pKa = 10.63LSAVEE46 pKa = 3.93NEE48 pKa = 4.34EE49 pKa = 4.03YY50 pKa = 10.46TFQSSILLEE59 pKa = 4.04NDD61 pKa = 3.57EE62 pKa = 4.6IVDD65 pKa = 3.47NARR68 pKa = 11.84IKK70 pKa = 11.2DD71 pKa = 3.76FDD73 pKa = 3.98NEE75 pKa = 4.15TTAGGSITDD84 pKa = 4.08NRR86 pKa = 11.84DD87 pKa = 2.5GTYY90 pKa = 10.02TYY92 pKa = 9.91TPPADD97 pKa = 3.88FKK99 pKa = 11.73GDD101 pKa = 3.53DD102 pKa = 3.61TFDD105 pKa = 3.47YY106 pKa = 9.13TLCVPGDD113 pKa = 3.84SNRR116 pKa = 11.84CSTATVTISVGDD128 pKa = 3.7AGDD131 pKa = 3.57PVAIDD136 pKa = 4.19DD137 pKa = 4.47SYY139 pKa = 11.68QINEE143 pKa = 4.13NEE145 pKa = 4.38SYY147 pKa = 10.55TIRR150 pKa = 11.84NYY152 pKa = 11.22LNNDD156 pKa = 3.71DD157 pKa = 5.38LKK159 pKa = 11.67DD160 pKa = 3.65NATVTDD166 pKa = 3.8VLSEE170 pKa = 4.22SGNATVTLQEE180 pKa = 4.98DD181 pKa = 4.04GSIRR185 pKa = 11.84YY186 pKa = 8.56VPNEE190 pKa = 4.1HH191 pKa = 6.97FSGEE195 pKa = 4.09DD196 pKa = 2.98TFTYY200 pKa = 8.17TICDD204 pKa = 3.61DD205 pKa = 5.44DD206 pKa = 4.01EE207 pKa = 4.66TPNCSTATITMTVVDD222 pKa = 4.54EE223 pKa = 4.69GSPKK227 pKa = 10.79AEE229 pKa = 4.56DD230 pKa = 3.71DD231 pKa = 3.95SVIIASGTTEE241 pKa = 3.95TTFKK245 pKa = 11.25NLLDD249 pKa = 4.31NDD251 pKa = 4.5DD252 pKa = 5.55LIDD255 pKa = 4.23DD256 pKa = 4.12AVITSVEE263 pKa = 4.14STGNGTATLNEE274 pKa = 4.88DD275 pKa = 3.32GTVTFEE281 pKa = 4.05PQAGFSGDD289 pKa = 3.47DD290 pKa = 3.22TFTYY294 pKa = 8.15TICDD298 pKa = 3.65DD299 pKa = 5.88DD300 pKa = 4.63VDD302 pKa = 4.36QFCSTATVTVSVVEE316 pKa = 4.31SYY318 pKa = 11.33GFNIPADD325 pKa = 3.6LEE327 pKa = 4.45YY328 pKa = 10.86YY329 pKa = 10.58YY330 pKa = 11.44GDD332 pKa = 4.49AIFAKK337 pKa = 10.58GGGTIAYY344 pKa = 9.24QLLSDD349 pKa = 4.72FTNAMHH355 pKa = 6.05TTHH358 pKa = 7.36LEE360 pKa = 3.77YY361 pKa = 10.27TDD363 pKa = 3.42RR364 pKa = 11.84HH365 pKa = 6.45DD366 pKa = 3.97YY367 pKa = 11.13LYY369 pKa = 11.14DD370 pKa = 4.4ADD372 pKa = 5.65ADD374 pKa = 3.94PADD377 pKa = 4.1PSKK380 pKa = 11.17VILMYY385 pKa = 10.62SGEE388 pKa = 4.34SRR390 pKa = 11.84PDD392 pKa = 3.19DD393 pKa = 3.26QYY395 pKa = 12.02QLHH398 pKa = 6.44EE399 pKa = 4.53PPLRR403 pKa = 11.84GEE405 pKa = 4.39TFNTEE410 pKa = 3.91HH411 pKa = 7.62IYY413 pKa = 9.98PQSKK417 pKa = 9.96LDD419 pKa = 3.69HH420 pKa = 7.43DD421 pKa = 4.11EE422 pKa = 4.23AANDD426 pKa = 3.7LHH428 pKa = 7.69HH429 pKa = 6.62MRR431 pKa = 11.84VADD434 pKa = 3.17IDD436 pKa = 4.32VNSEE440 pKa = 3.26RR441 pKa = 11.84SYY443 pKa = 11.87YY444 pKa = 10.18PFTDD448 pKa = 3.32GTGTYY453 pKa = 10.8KK454 pKa = 10.87LVDD457 pKa = 3.63GNKK460 pKa = 8.95WFPGDD465 pKa = 3.52DD466 pKa = 3.11WRR468 pKa = 11.84GDD470 pKa = 3.45VARR473 pKa = 11.84MVMYY477 pKa = 11.02VNLAYY482 pKa = 10.23GDD484 pKa = 4.02SFDD487 pKa = 4.35EE488 pKa = 4.47VGSKK492 pKa = 10.92DD493 pKa = 6.14LFLKK497 pKa = 9.84WNRR500 pKa = 11.84EE501 pKa = 4.0DD502 pKa = 3.58PVSEE506 pKa = 5.39FEE508 pKa = 3.92IQRR511 pKa = 11.84NNVIEE516 pKa = 4.38GAQGNRR522 pKa = 11.84NPFIDD527 pKa = 3.96NPYY530 pKa = 10.19LATLIWGGEE539 pKa = 3.84PAQNRR544 pKa = 11.84WQQ546 pKa = 3.34

Molecular weight: 60.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R3Z8W2|A0A2R3Z8W2_9FLAO Chalcone isomerase OS=Gramella fulva OX=2126553 GN=C7S20_15985 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.15RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84KK13 pKa = 8.29NKK15 pKa = 9.34HH16 pKa = 4.05GFRR19 pKa = 11.84EE20 pKa = 4.21RR21 pKa = 11.84MSSANGRR28 pKa = 11.84KK29 pKa = 8.88VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.09GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.73LSVSSEE47 pKa = 3.76NRR49 pKa = 11.84HH50 pKa = 4.47KK51 pKa = 10.5HH52 pKa = 4.49

Molecular weight: 6.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3785

0

3785

1310285

25

12288

346.2

39.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.389 ± 0.05

0.697 ± 0.015

5.601 ± 0.038

7.354 ± 0.058

5.235 ± 0.036

6.605 ± 0.041

1.822 ± 0.023

7.593 ± 0.037

7.83 ± 0.077

9.331 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.279 ± 0.028

5.7 ± 0.047

3.648 ± 0.021

3.383 ± 0.023

3.92 ± 0.048

6.428 ± 0.051

5.123 ± 0.116

5.898 ± 0.037

1.152 ± 0.02

4.013 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski