Escherichia phage myPSH1131

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Kuravirus; unclassified Kuravirus

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R3U9Z3|A0A2R3U9Z3_9CAUD Uncharacterized protein OS=Escherichia phage myPSH1131 OX=2108117 GN=PSH1131_033 PE=4 SV=1
MM1 pKa = 7.56KK2 pKa = 10.15KK3 pKa = 9.84YY4 pKa = 10.59SVWINEE10 pKa = 4.02EE11 pKa = 3.92EE12 pKa = 4.18EE13 pKa = 4.67GFDD16 pKa = 4.87EE17 pKa = 5.33AGGWVNILNTDD28 pKa = 4.28DD29 pKa = 3.89IEE31 pKa = 4.23EE32 pKa = 4.36AEE34 pKa = 4.34KK35 pKa = 11.04CALNYY40 pKa = 10.53NSGYY44 pKa = 8.91YY45 pKa = 8.57EE46 pKa = 4.26RR47 pKa = 11.84NWEE50 pKa = 4.11YY51 pKa = 11.11EE52 pKa = 4.04EE53 pKa = 4.84EE54 pKa = 4.23EE55 pKa = 4.18

Molecular weight: 6.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R3UA52|A0A2R3UA52_9CAUD Type I DNA polymerase OS=Escherichia phage myPSH1131 OX=2108117 GN=PSH1131_067 PE=4 SV=1
MM1 pKa = 7.76RR2 pKa = 11.84PITMRR7 pKa = 11.84KK8 pKa = 8.37FWLAKK13 pKa = 9.99IKK15 pKa = 9.23WASGGMTMTALPTRR29 pKa = 11.84QQARR33 pKa = 11.84DD34 pKa = 3.7FKK36 pKa = 10.51KK37 pKa = 9.3TMLTGDD43 pKa = 3.86VVNSISIHH51 pKa = 5.12KK52 pKa = 9.92AKK54 pKa = 9.84EE55 pKa = 3.85YY56 pKa = 10.59SSGRR60 pKa = 11.84VYY62 pKa = 10.76ISGKK66 pKa = 7.19VHH68 pKa = 5.8YY69 pKa = 10.08

Molecular weight: 7.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

12594

26

602

135.4

15.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.551 ± 0.506

1.128 ± 0.151

5.669 ± 0.302

6.503 ± 0.394

3.891 ± 0.209

7.504 ± 0.471

2.025 ± 0.204

5.717 ± 0.192

6.233 ± 0.494

7.599 ± 0.22

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.049 ± 0.164

6.082 ± 0.428

3.549 ± 0.248

4.963 ± 0.603

5.217 ± 0.306

6.273 ± 0.251

5.129 ± 0.182

6.511 ± 0.296

1.652 ± 0.108

3.756 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski