Amino acid dipepetide frequency for Escherichia phage myPSH1131

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.703AlaAla: 7.703 ± 1.011
0.715AlaCys: 0.715 ± 0.257
4.288AlaAsp: 4.288 ± 0.55
4.368AlaGlu: 4.368 ± 0.733
1.985AlaPhe: 1.985 ± 0.348
6.115AlaGly: 6.115 ± 1.01
1.032AlaHis: 1.032 ± 0.256
4.685AlaIle: 4.685 ± 0.51
4.129AlaLys: 4.129 ± 0.746
8.338AlaLeu: 8.338 ± 1.026
2.065AlaMet: 2.065 ± 0.398
3.732AlaAsn: 3.732 ± 0.565
2.7AlaPro: 2.7 ± 0.504
4.844AlaGln: 4.844 ± 0.942
3.653AlaArg: 3.653 ± 0.517
4.05AlaSer: 4.05 ± 0.627
3.256AlaThr: 3.256 ± 0.56
4.685AlaVal: 4.685 ± 0.609
1.191AlaTrp: 1.191 ± 0.246
1.985AlaTyr: 1.985 ± 0.449
0.0AlaXaa: 0.0 ± 0.0
Cys
1.032CysAla: 1.032 ± 0.351
0.0CysCys: 0.0 ± 0.0
0.397CysAsp: 0.397 ± 0.21
0.953CysGlu: 0.953 ± 0.247
0.318CysPhe: 0.318 ± 0.15
1.112CysGly: 1.112 ± 0.282
0.318CysHis: 0.318 ± 0.175
0.476CysIle: 0.476 ± 0.185
0.635CysLys: 0.635 ± 0.26
0.874CysLeu: 0.874 ± 0.242
0.159CysMet: 0.159 ± 0.108
0.318CysAsn: 0.318 ± 0.179
0.556CysPro: 0.556 ± 0.254
0.318CysGln: 0.318 ± 0.177
0.635CysArg: 0.635 ± 0.231
0.794CysSer: 0.794 ± 0.276
0.556CysThr: 0.556 ± 0.175
1.35CysVal: 1.35 ± 0.303
0.079CysTrp: 0.079 ± 0.078
0.397CysTyr: 0.397 ± 0.178
0.0CysXaa: 0.0 ± 0.0
Asp
4.526AspAla: 4.526 ± 0.572
0.476AspCys: 0.476 ± 0.198
2.938AspAsp: 2.938 ± 0.439
3.891AspGlu: 3.891 ± 0.469
1.271AspPhe: 1.271 ± 0.277
4.368AspGly: 4.368 ± 0.651
1.588AspHis: 1.588 ± 0.426
4.129AspIle: 4.129 ± 0.568
3.653AspLys: 3.653 ± 0.538
4.844AspLeu: 4.844 ± 0.7
2.065AspMet: 2.065 ± 0.431
2.541AspAsn: 2.541 ± 0.396
2.621AspPro: 2.621 ± 0.636
1.826AspGln: 1.826 ± 0.405
2.859AspArg: 2.859 ± 0.432
3.494AspSer: 3.494 ± 0.534
2.779AspThr: 2.779 ± 0.421
3.176AspVal: 3.176 ± 0.499
1.112AspTrp: 1.112 ± 0.288
2.541AspTyr: 2.541 ± 0.365
0.0AspXaa: 0.0 ± 0.0
Glu
5.082GluAla: 5.082 ± 0.66
1.032GluCys: 1.032 ± 0.334
3.812GluAsp: 3.812 ± 0.561
6.194GluGlu: 6.194 ± 0.995
2.541GluPhe: 2.541 ± 0.483
3.653GluGly: 3.653 ± 0.658
1.509GluHis: 1.509 ± 0.387
3.256GluIle: 3.256 ± 0.443
4.526GluLys: 4.526 ± 0.622
4.844GluLeu: 4.844 ± 0.707
2.779GluMet: 2.779 ± 0.543
3.176GluAsn: 3.176 ± 0.537
1.906GluPro: 1.906 ± 0.397
3.335GluGln: 3.335 ± 0.536
3.415GluArg: 3.415 ± 0.662
3.494GluSer: 3.494 ± 0.472
2.223GluThr: 2.223 ± 0.413
4.526GluVal: 4.526 ± 0.616
1.429GluTrp: 1.429 ± 0.307
2.223GluTyr: 2.223 ± 0.396
0.0GluXaa: 0.0 ± 0.0
Phe
1.985PheAla: 1.985 ± 0.397
0.953PheCys: 0.953 ± 0.255
3.415PheAsp: 3.415 ± 0.576
2.462PheGlu: 2.462 ± 0.42
1.668PhePhe: 1.668 ± 0.392
1.985PheGly: 1.985 ± 0.327
0.794PheHis: 0.794 ± 0.292
2.065PheIle: 2.065 ± 0.394
2.223PheLys: 2.223 ± 0.473
2.621PheLeu: 2.621 ± 0.565
0.715PheMet: 0.715 ± 0.255
3.335PheAsn: 3.335 ± 0.453
1.032PhePro: 1.032 ± 0.366
1.826PheGln: 1.826 ± 0.405
1.826PheArg: 1.826 ± 0.371
2.859PheSer: 2.859 ± 0.407
2.462PheThr: 2.462 ± 0.403
2.382PheVal: 2.382 ± 0.429
0.556PheTrp: 0.556 ± 0.21
1.747PheTyr: 1.747 ± 0.442
0.0PheXaa: 0.0 ± 0.0
Gly
6.115GlyAla: 6.115 ± 1.163
0.953GlyCys: 0.953 ± 0.309
4.288GlyAsp: 4.288 ± 0.606
3.415GlyGlu: 3.415 ± 0.677
2.859GlyPhe: 2.859 ± 0.399
6.353GlyGly: 6.353 ± 0.859
1.191GlyHis: 1.191 ± 0.255
4.209GlyIle: 4.209 ± 0.503
5.32GlyLys: 5.32 ± 0.713
6.829GlyLeu: 6.829 ± 0.967
2.144GlyMet: 2.144 ± 0.409
3.573GlyAsn: 3.573 ± 0.55
1.271GlyPro: 1.271 ± 0.284
4.368GlyGln: 4.368 ± 0.824
3.335GlyArg: 3.335 ± 0.495
6.67GlySer: 6.67 ± 0.875
2.382GlyThr: 2.382 ± 0.458
5.32GlyVal: 5.32 ± 0.595
1.429GlyTrp: 1.429 ± 0.323
3.018GlyTyr: 3.018 ± 0.407
0.0GlyXaa: 0.0 ± 0.0
His
0.874HisAla: 0.874 ± 0.319
0.079HisCys: 0.079 ± 0.07
1.35HisAsp: 1.35 ± 0.351
1.271HisGlu: 1.271 ± 0.353
0.715HisPhe: 0.715 ± 0.217
0.794HisGly: 0.794 ± 0.235
0.715HisHis: 0.715 ± 0.201
1.191HisIle: 1.191 ± 0.317
1.35HisLys: 1.35 ± 0.354
1.588HisLeu: 1.588 ± 0.302
0.635HisMet: 0.635 ± 0.189
1.35HisAsn: 1.35 ± 0.276
0.794HisPro: 0.794 ± 0.225
0.476HisGln: 0.476 ± 0.168
1.35HisArg: 1.35 ± 0.373
1.588HisSer: 1.588 ± 0.415
1.191HisThr: 1.191 ± 0.342
1.271HisVal: 1.271 ± 0.321
0.318HisTrp: 0.318 ± 0.141
1.35HisTyr: 1.35 ± 0.375
0.0HisXaa: 0.0 ± 0.0
Ile
2.938IleAla: 2.938 ± 0.39
0.794IleCys: 0.794 ± 0.321
3.415IleAsp: 3.415 ± 0.454
3.732IleGlu: 3.732 ± 0.687
1.668IlePhe: 1.668 ± 0.408
4.685IleGly: 4.685 ± 0.653
0.794IleHis: 0.794 ± 0.275
2.7IleIle: 2.7 ± 0.447
4.129IleLys: 4.129 ± 0.593
3.732IleLeu: 3.732 ± 0.499
1.35IleMet: 1.35 ± 0.311
3.97IleAsn: 3.97 ± 0.595
3.335IlePro: 3.335 ± 0.575
1.906IleGln: 1.906 ± 0.352
3.256IleArg: 3.256 ± 0.609
4.05IleSer: 4.05 ± 0.48
4.209IleThr: 4.209 ± 0.551
3.335IleVal: 3.335 ± 0.453
0.715IleTrp: 0.715 ± 0.285
2.462IleTyr: 2.462 ± 0.472
0.0IleXaa: 0.0 ± 0.0
Lys
4.923LysAla: 4.923 ± 0.69
0.476LysCys: 0.476 ± 0.233
4.288LysAsp: 4.288 ± 0.621
5.638LysGlu: 5.638 ± 0.822
1.906LysPhe: 1.906 ± 0.362
4.606LysGly: 4.606 ± 0.568
2.144LysHis: 2.144 ± 0.378
2.621LysIle: 2.621 ± 0.476
4.526LysLys: 4.526 ± 0.722
3.812LysLeu: 3.812 ± 0.635
1.668LysMet: 1.668 ± 0.374
3.176LysAsn: 3.176 ± 0.598
2.223LysPro: 2.223 ± 0.516
2.938LysGln: 2.938 ± 0.412
2.7LysArg: 2.7 ± 0.666
3.653LysSer: 3.653 ± 0.547
3.256LysThr: 3.256 ± 0.557
3.891LysVal: 3.891 ± 0.612
1.35LysTrp: 1.35 ± 0.326
2.541LysTyr: 2.541 ± 0.47
0.0LysXaa: 0.0 ± 0.0
Leu
7.226LeuAla: 7.226 ± 0.806
1.429LeuCys: 1.429 ± 0.373
3.018LeuAsp: 3.018 ± 0.523
5.479LeuGlu: 5.479 ± 0.729
3.732LeuPhe: 3.732 ± 0.503
6.353LeuGly: 6.353 ± 0.85
1.112LeuHis: 1.112 ± 0.334
3.891LeuIle: 3.891 ± 0.644
3.732LeuLys: 3.732 ± 0.558
4.923LeuLeu: 4.923 ± 0.591
2.462LeuMet: 2.462 ± 0.371
5.717LeuAsn: 5.717 ± 0.959
2.621LeuPro: 2.621 ± 0.348
2.859LeuGln: 2.859 ± 0.388
3.891LeuArg: 3.891 ± 0.565
5.559LeuSer: 5.559 ± 0.897
3.812LeuThr: 3.812 ± 0.477
5.479LeuVal: 5.479 ± 0.787
0.397LeuTrp: 0.397 ± 0.194
2.303LeuTyr: 2.303 ± 0.438
0.0LeuXaa: 0.0 ± 0.0
Met
2.223MetAla: 2.223 ± 0.416
0.318MetCys: 0.318 ± 0.155
1.112MetAsp: 1.112 ± 0.307
2.621MetGlu: 2.621 ± 0.42
1.747MetPhe: 1.747 ± 0.348
1.588MetGly: 1.588 ± 0.345
0.715MetHis: 0.715 ± 0.317
1.826MetIle: 1.826 ± 0.322
3.176MetLys: 3.176 ± 0.573
1.588MetLeu: 1.588 ± 0.35
1.112MetMet: 1.112 ± 0.284
1.668MetAsn: 1.668 ± 0.372
0.794MetPro: 0.794 ± 0.259
1.032MetGln: 1.032 ± 0.235
2.303MetArg: 2.303 ± 0.479
1.985MetSer: 1.985 ± 0.323
1.509MetThr: 1.509 ± 0.417
1.509MetVal: 1.509 ± 0.342
0.476MetTrp: 0.476 ± 0.192
1.191MetTyr: 1.191 ± 0.276
0.0MetXaa: 0.0 ± 0.0
Asn
5.003AsnAla: 5.003 ± 0.637
0.0AsnCys: 0.0 ± 0.0
2.223AsnAsp: 2.223 ± 0.414
2.779AsnGlu: 2.779 ± 0.464
3.176AsnPhe: 3.176 ± 0.599
4.526AsnGly: 4.526 ± 0.607
1.112AsnHis: 1.112 ± 0.29
3.812AsnIle: 3.812 ± 0.525
3.415AsnLys: 3.415 ± 0.509
5.162AsnLeu: 5.162 ± 0.746
1.747AsnMet: 1.747 ± 0.402
4.05AsnAsn: 4.05 ± 0.827
3.018AsnPro: 3.018 ± 0.53
3.732AsnGln: 3.732 ± 0.761
3.018AsnArg: 3.018 ± 0.486
3.97AsnSer: 3.97 ± 0.592
3.335AsnThr: 3.335 ± 0.444
3.494AsnVal: 3.494 ± 0.409
1.191AsnTrp: 1.191 ± 0.321
2.065AsnTyr: 2.065 ± 0.391
0.0AsnXaa: 0.0 ± 0.0
Pro
2.382ProAla: 2.382 ± 0.41
0.238ProCys: 0.238 ± 0.123
2.382ProAsp: 2.382 ± 0.437
2.303ProGlu: 2.303 ± 0.354
1.35ProPhe: 1.35 ± 0.302
1.588ProGly: 1.588 ± 0.335
0.715ProHis: 0.715 ± 0.206
2.065ProIle: 2.065 ± 0.392
2.065ProLys: 2.065 ± 0.439
2.859ProLeu: 2.859 ± 0.485
1.668ProMet: 1.668 ± 0.346
1.668ProAsn: 1.668 ± 0.394
2.144ProPro: 2.144 ± 0.578
1.032ProGln: 1.032 ± 0.3
1.668ProArg: 1.668 ± 0.36
2.144ProSer: 2.144 ± 0.535
2.541ProThr: 2.541 ± 0.586
3.335ProVal: 3.335 ± 0.609
0.556ProTrp: 0.556 ± 0.186
0.794ProTyr: 0.794 ± 0.27
0.0ProXaa: 0.0 ± 0.0
Gln
4.526GlnAla: 4.526 ± 0.869
1.032GlnCys: 1.032 ± 0.326
2.462GlnAsp: 2.462 ± 0.471
2.303GlnGlu: 2.303 ± 0.475
1.668GlnPhe: 1.668 ± 0.322
3.732GlnGly: 3.732 ± 0.779
0.159GlnHis: 0.159 ± 0.126
2.7GlnIle: 2.7 ± 0.446
2.621GlnLys: 2.621 ± 0.408
3.732GlnLeu: 3.732 ± 0.883
1.191GlnMet: 1.191 ± 0.295
4.129GlnAsn: 4.129 ± 0.907
0.953GlnPro: 0.953 ± 0.263
6.273GlnGln: 6.273 ± 1.945
2.621GlnArg: 2.621 ± 0.447
2.065GlnSer: 2.065 ± 0.464
2.144GlnThr: 2.144 ± 0.384
2.7GlnVal: 2.7 ± 0.414
0.874GlnTrp: 0.874 ± 0.225
1.747GlnTyr: 1.747 ± 0.431
0.0GlnXaa: 0.0 ± 0.0
Arg
4.05ArgAla: 4.05 ± 0.758
0.635ArgCys: 0.635 ± 0.24
4.05ArgAsp: 4.05 ± 0.603
3.573ArgGlu: 3.573 ± 0.58
1.985ArgPhe: 1.985 ± 0.416
3.256ArgGly: 3.256 ± 0.617
0.874ArgHis: 0.874 ± 0.258
2.382ArgIle: 2.382 ± 0.454
3.732ArgLys: 3.732 ± 0.595
3.176ArgLeu: 3.176 ± 0.567
1.668ArgMet: 1.668 ± 0.314
2.541ArgAsn: 2.541 ± 0.609
1.588ArgPro: 1.588 ± 0.341
2.382ArgGln: 2.382 ± 0.465
3.653ArgArg: 3.653 ± 0.563
3.415ArgSer: 3.415 ± 0.469
2.779ArgThr: 2.779 ± 0.482
3.573ArgVal: 3.573 ± 0.628
0.953ArgTrp: 0.953 ± 0.273
1.906ArgTyr: 1.906 ± 0.346
0.0ArgXaa: 0.0 ± 0.0
Ser
3.97SerAla: 3.97 ± 0.579
0.397SerCys: 0.397 ± 0.206
3.256SerAsp: 3.256 ± 0.448
3.256SerGlu: 3.256 ± 0.522
2.938SerPhe: 2.938 ± 0.512
7.067SerGly: 7.067 ± 1.043
1.747SerHis: 1.747 ± 0.377
3.176SerIle: 3.176 ± 0.567
3.097SerLys: 3.097 ± 0.517
5.003SerLeu: 5.003 ± 0.528
1.747SerMet: 1.747 ± 0.392
4.923SerAsn: 4.923 ± 0.734
2.621SerPro: 2.621 ± 0.463
2.779SerGln: 2.779 ± 0.654
2.779SerArg: 2.779 ± 0.475
3.256SerSer: 3.256 ± 0.529
3.256SerThr: 3.256 ± 0.493
3.176SerVal: 3.176 ± 0.479
1.271SerTrp: 1.271 ± 0.305
3.018SerTyr: 3.018 ± 0.518
0.0SerXaa: 0.0 ± 0.0
Thr
3.256ThrAla: 3.256 ± 0.512
0.318ThrCys: 0.318 ± 0.162
2.621ThrAsp: 2.621 ± 0.472
3.097ThrGlu: 3.097 ± 0.476
2.462ThrPhe: 2.462 ± 0.486
4.368ThrGly: 4.368 ± 0.449
1.112ThrHis: 1.112 ± 0.334
3.653ThrIle: 3.653 ± 0.553
2.7ThrLys: 2.7 ± 0.419
4.129ThrLeu: 4.129 ± 0.641
1.35ThrMet: 1.35 ± 0.407
3.256ThrAsn: 3.256 ± 0.485
2.065ThrPro: 2.065 ± 0.421
2.303ThrGln: 2.303 ± 0.477
2.382ThrArg: 2.382 ± 0.393
3.176ThrSer: 3.176 ± 0.528
2.144ThrThr: 2.144 ± 0.415
3.653ThrVal: 3.653 ± 0.668
1.032ThrTrp: 1.032 ± 0.325
2.223ThrTyr: 2.223 ± 0.385
0.0ThrXaa: 0.0 ± 0.0
Val
4.685ValAla: 4.685 ± 0.583
0.635ValCys: 0.635 ± 0.222
3.653ValAsp: 3.653 ± 0.521
4.606ValGlu: 4.606 ± 0.7
3.256ValPhe: 3.256 ± 0.598
5.162ValGly: 5.162 ± 0.682
1.588ValHis: 1.588 ± 0.304
4.129ValIle: 4.129 ± 0.535
3.891ValLys: 3.891 ± 0.514
3.97ValLeu: 3.97 ± 0.581
1.35ValMet: 1.35 ± 0.421
4.606ValAsn: 4.606 ± 0.511
1.826ValPro: 1.826 ± 0.537
2.7ValGln: 2.7 ± 0.462
3.573ValArg: 3.573 ± 0.557
2.859ValSer: 2.859 ± 0.42
4.209ValThr: 4.209 ± 0.678
5.082ValVal: 5.082 ± 0.764
1.112ValTrp: 1.112 ± 0.312
2.223ValTyr: 2.223 ± 0.438
0.0ValXaa: 0.0 ± 0.0
Trp
0.953TrpAla: 0.953 ± 0.226
0.159TrpCys: 0.159 ± 0.114
1.429TrpAsp: 1.429 ± 0.291
0.715TrpGlu: 0.715 ± 0.243
0.635TrpPhe: 0.635 ± 0.242
1.271TrpGly: 1.271 ± 0.315
0.159TrpHis: 0.159 ± 0.116
1.35TrpIle: 1.35 ± 0.288
0.953TrpLys: 0.953 ± 0.296
1.112TrpLeu: 1.112 ± 0.303
0.953TrpMet: 0.953 ± 0.287
1.191TrpAsn: 1.191 ± 0.265
0.318TrpPro: 0.318 ± 0.19
0.874TrpGln: 0.874 ± 0.251
0.715TrpArg: 0.715 ± 0.2
0.874TrpSer: 0.874 ± 0.257
1.271TrpThr: 1.271 ± 0.331
1.112TrpVal: 1.112 ± 0.309
0.159TrpTrp: 0.159 ± 0.105
0.318TrpTyr: 0.318 ± 0.201
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.065TyrAla: 2.065 ± 0.354
0.635TyrCys: 0.635 ± 0.198
2.303TyrAsp: 2.303 ± 0.491
2.382TyrGlu: 2.382 ± 0.448
1.032TyrPhe: 1.032 ± 0.333
2.462TyrGly: 2.462 ± 0.509
0.874TyrHis: 0.874 ± 0.244
2.859TyrIle: 2.859 ± 0.443
2.462TyrLys: 2.462 ± 0.537
2.859TyrLeu: 2.859 ± 0.5
1.588TyrMet: 1.588 ± 0.338
1.906TyrAsn: 1.906 ± 0.346
1.112TyrPro: 1.112 ± 0.351
1.826TyrGln: 1.826 ± 0.372
2.541TyrArg: 2.541 ± 0.435
2.779TyrSer: 2.779 ± 0.529
1.985TyrThr: 1.985 ± 0.454
2.065TyrVal: 2.065 ± 0.369
0.318TyrTrp: 0.318 ± 0.15
1.509TyrTyr: 1.509 ± 0.35
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 93 proteins (12594 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski