Marinomonas hwangdonensis
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3066 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3M8Q5I6|A0A3M8Q5I6_9GAMM zinc-ribbon_6 domain-containing protein OS=Marinomonas hwangdonensis OX=1053647 GN=EBI00_05705 PE=4 SV=1
MM1 pKa = 7.06 AQSSIALIYY10 pKa = 9.31 GTDD13 pKa = 3.38 TNNTEE18 pKa = 4.28 EE19 pKa = 4.31 VGHH22 pKa = 6.78 KK23 pKa = 9.29 IAKK26 pKa = 8.28 QWQEE30 pKa = 3.55 LGEE33 pKa = 4.25 TVDD36 pKa = 3.71 IFNIKK41 pKa = 10.21 DD42 pKa = 3.12 IEE44 pKa = 4.14 LQQLEE49 pKa = 4.9 DD50 pKa = 3.9 YY51 pKa = 11.64 SMLILGIPTWDD62 pKa = 3.3 FGGIQSDD69 pKa = 3.69 WEE71 pKa = 4.74 DD72 pKa = 2.98 IGGSLAEE79 pKa = 4.65 LSLNDD84 pKa = 3.95 TVIALYY90 pKa = 10.93 GLGDD94 pKa = 3.4 QFGYY98 pKa = 11.1 GDD100 pKa = 3.99 YY101 pKa = 11.02 FIDD104 pKa = 3.75 AVGWLYY110 pKa = 11.27 EE111 pKa = 4.08 KK112 pKa = 10.65 LQPTNATFIGQWPTEE127 pKa = 4.18 GYY129 pKa = 10.69 DD130 pKa = 3.74 FEE132 pKa = 5.58 ASRR135 pKa = 11.84 ACIHH139 pKa = 6.89 DD140 pKa = 3.77 KK141 pKa = 10.83 EE142 pKa = 4.82 HH143 pKa = 6.57 FVGLAIDD150 pKa = 4.13 EE151 pKa = 4.54 DD152 pKa = 4.12 QQFEE156 pKa = 4.42 LTDD159 pKa = 3.33 QRR161 pKa = 11.84 IEE163 pKa = 3.62 QWVIQLYY170 pKa = 10.31 AEE172 pKa = 4.28 RR173 pKa = 11.84 TLEE176 pKa = 4.12 VAA178 pKa = 4.83
Molecular weight: 20.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.751
IPC2_protein 3.846
IPC_protein 3.821
Toseland 3.63
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.706
Rodwell 3.656
Grimsley 3.541
Solomon 3.795
Lehninger 3.745
Nozaki 3.91
DTASelect 4.101
Thurlkill 3.668
EMBOSS 3.719
Sillero 3.935
Patrickios 1.875
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.848
Protein with the highest isoelectric point:
>tr|A0A3M8Q6P5|A0A3M8Q6P5_9GAMM QacE family quaternary ammonium compound efflux SMR transporter OS=Marinomonas hwangdonensis OX=1053647 GN=EBI00_07640 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 9.37 RR14 pKa = 11.84 NHH16 pKa = 5.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.12 SGRR28 pKa = 11.84 QIVARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.91 VLSAA44 pKa = 4.11
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3066
0
3066
1026813
36
4153
334.9
37.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.96 ± 0.048
1.005 ± 0.015
5.604 ± 0.037
6.114 ± 0.047
4.129 ± 0.032
6.852 ± 0.048
2.286 ± 0.025
6.27 ± 0.038
5.05 ± 0.041
10.603 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.667 ± 0.021
4.011 ± 0.031
3.948 ± 0.029
4.422 ± 0.037
4.754 ± 0.036
6.745 ± 0.039
5.435 ± 0.047
7.107 ± 0.039
1.211 ± 0.018
2.826 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here