Blastococcus saxobsidens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Geodermatophilales; Geodermatophilaceae; Blastococcus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4219 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q7Y867|A0A4Q7Y867_9ACTN Glucose/arabinose dehydrogenase OS=Blastococcus saxobsidens OX=138336 GN=BKA19_2973 PE=4 SV=1
MM1 pKa = 7.35RR2 pKa = 11.84QLLRR6 pKa = 11.84RR7 pKa = 11.84AVPALLAGVLVAGCAVDD24 pKa = 3.81VVRR27 pKa = 11.84GEE29 pKa = 4.22ATPGTSGPTDD39 pKa = 2.84IGVEE43 pKa = 4.08EE44 pKa = 4.77FPVTGASDD52 pKa = 3.61EE53 pKa = 5.32PIDD56 pKa = 3.86QFARR60 pKa = 11.84NALADD65 pKa = 4.96LEE67 pKa = 5.53SFWQTAYY74 pKa = 9.88PAFFGAEE81 pKa = 4.01YY82 pKa = 10.45QPLAGGYY89 pKa = 9.75FSVDD93 pKa = 3.32SVDD96 pKa = 4.76LDD98 pKa = 3.37ASAYY102 pKa = 8.59PEE104 pKa = 4.08TGIGCEE110 pKa = 4.25GSPTAPEE117 pKa = 4.17DD118 pKa = 3.48VAGNAFYY125 pKa = 11.11DD126 pKa = 4.06PVCDD130 pKa = 4.21VIAYY134 pKa = 8.88DD135 pKa = 3.53RR136 pKa = 11.84TLLQEE141 pKa = 4.22LADD144 pKa = 4.3DD145 pKa = 4.16YY146 pKa = 11.83GRR148 pKa = 11.84FLAPVVMAHH157 pKa = 6.21EE158 pKa = 5.55FGHH161 pKa = 6.59AMQGRR166 pKa = 11.84FGFAASGRR174 pKa = 11.84SILDD178 pKa = 3.22EE179 pKa = 4.19TQADD183 pKa = 4.22CLAGAWTRR191 pKa = 11.84WVADD195 pKa = 3.59GQARR199 pKa = 11.84YY200 pKa = 8.0VTLRR204 pKa = 11.84EE205 pKa = 4.13PEE207 pKa = 4.12LDD209 pKa = 3.52DD210 pKa = 3.65VVRR213 pKa = 11.84GFLLLRR219 pKa = 11.84DD220 pKa = 4.48DD221 pKa = 4.65VGSDD225 pKa = 3.54PDD227 pKa = 3.46DD228 pKa = 4.2TEE230 pKa = 4.14AHH232 pKa = 6.24GSFFDD237 pKa = 3.69RR238 pKa = 11.84VSAFSDD244 pKa = 3.73GFDD247 pKa = 3.64GGLAVCRR254 pKa = 11.84DD255 pKa = 3.62EE256 pKa = 5.35FGEE259 pKa = 4.57DD260 pKa = 3.14RR261 pKa = 11.84LFTAAAFTPTDD272 pKa = 3.78EE273 pKa = 4.99LSQGNAPFADD283 pKa = 3.12IVDD286 pKa = 4.3WVATTLLAFWAEE298 pKa = 4.12AFPQTFGGEE307 pKa = 4.19FEE309 pKa = 4.42PPALEE314 pKa = 4.33GFGGGAPDD322 pKa = 4.94CDD324 pKa = 3.71GLDD327 pKa = 3.56GRR329 pKa = 11.84RR330 pKa = 11.84LGYY333 pKa = 10.2CADD336 pKa = 3.71EE337 pKa = 4.04ATVYY341 pKa = 10.79VDD343 pKa = 3.48EE344 pKa = 5.12TEE346 pKa = 4.43LAVPAYY352 pKa = 10.64DD353 pKa = 3.6EE354 pKa = 4.67VGDD357 pKa = 4.11FALTTALSLPYY368 pKa = 10.34ALAVRR373 pKa = 11.84DD374 pKa = 3.78QAGLSVDD381 pKa = 4.46DD382 pKa = 4.59GAATRR387 pKa = 11.84SAVCLTGWYY396 pKa = 7.3EE397 pKa = 4.1AQWYY401 pKa = 7.74TDD403 pKa = 3.15AFADD407 pKa = 4.58LVPAQISPGDD417 pKa = 3.44IDD419 pKa = 3.79EE420 pKa = 4.94AVQFLLQYY428 pKa = 10.92GVDD431 pKa = 3.89DD432 pKa = 3.82QVLPDD437 pKa = 3.4VDD439 pKa = 3.81ASGFEE444 pKa = 4.06LVGEE448 pKa = 4.23FRR450 pKa = 11.84SGFLYY455 pKa = 10.51GAEE458 pKa = 4.29GCGLGG463 pKa = 4.38

Molecular weight: 49.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q7YAU2|A0A4Q7YAU2_9ACTN Uncharacterized protein DUF4397 OS=Blastococcus saxobsidens OX=138336 GN=BKA19_3693 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.25TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.81KK32 pKa = 9.63

Molecular weight: 4.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4219

0

4219

1345434

30

1989

318.9

33.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.226 ± 0.063

0.677 ± 0.011

6.18 ± 0.031

5.674 ± 0.032

2.613 ± 0.022

9.71 ± 0.031

1.979 ± 0.019

2.91 ± 0.025

1.425 ± 0.024

10.716 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.684 ± 0.014

1.451 ± 0.016

6.176 ± 0.035

2.728 ± 0.021

8.091 ± 0.036

4.913 ± 0.023

5.921 ± 0.026

9.703 ± 0.04

1.465 ± 0.018

1.758 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski