Flavobacterium sp. CC-CTC003
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3402 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V3W7M5|A0A4V3W7M5_9FLAO Uncharacterized protein OS=Flavobacterium sp. CC-CTC003 OX=2565924 GN=E6C50_15940 PE=4 SV=1
MM1 pKa = 7.56 DD2 pKa = 5.2 TIKK5 pKa = 10.61 KK6 pKa = 8.64 VEE8 pKa = 4.07 RR9 pKa = 11.84 VEE11 pKa = 4.08 VLIAKK16 pKa = 9.94 LLEE19 pKa = 4.34 KK20 pKa = 10.68 YY21 pKa = 10.02 AAEE24 pKa = 4.21 GSNINRR30 pKa = 11.84 IPSPFIQEE38 pKa = 3.99 ALRR41 pKa = 11.84 LKK43 pKa = 9.84 DD44 pKa = 3.35 TAPFLDD50 pKa = 3.63 IDD52 pKa = 4.06 SYY54 pKa = 11.97 VYY56 pKa = 10.28 FLSEE60 pKa = 4.04 MSVLILDD67 pKa = 4.83 LGDD70 pKa = 3.33 GVYY73 pKa = 9.53 ATFYY77 pKa = 11.44 GLDD80 pKa = 3.5 DD81 pKa = 3.83 WEE83 pKa = 4.52 EE84 pKa = 3.93 GLNIFDD90 pKa = 4.01 YY91 pKa = 10.33 PIPEE95 pKa = 4.1 EE96 pKa = 4.14 NGFHH100 pKa = 6.88 LVLDD104 pKa = 3.9 ICNADD109 pKa = 3.36 GAITYY114 pKa = 8.94 FSYY117 pKa = 11.09 NSEE120 pKa = 4.12 NDD122 pKa = 3.25 NEE124 pKa = 4.76 DD125 pKa = 3.48 ILWISTNVEE134 pKa = 3.91 DD135 pKa = 5.45 GPYY138 pKa = 9.42 TKK140 pKa = 10.52 SDD142 pKa = 3.33 LSFVDD147 pKa = 3.94 VLQSIYY153 pKa = 11.02 DD154 pKa = 3.82 NNWNVVV160 pKa = 3.0
Molecular weight: 18.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.795
IPC_protein 3.783
Toseland 3.567
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.884
DTASelect 4.101
Thurlkill 3.617
EMBOSS 3.706
Sillero 3.897
Patrickios 1.036
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.821
Protein with the highest isoelectric point:
>tr|A0A4S3ZZR4|A0A4S3ZZR4_9FLAO Uncharacterized protein OS=Flavobacterium sp. CC-CTC003 OX=2565924 GN=E6C50_04950 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.13 RR11 pKa = 11.84 KK12 pKa = 9.48 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.53 HH17 pKa = 4.24 GFMEE21 pKa = 4.81 RR22 pKa = 11.84 MASANGRR29 pKa = 11.84 KK30 pKa = 9.04 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.19 GRR40 pKa = 11.84 HH41 pKa = 5.41 KK42 pKa = 10.14 LTVSSEE48 pKa = 3.92 PRR50 pKa = 11.84 HH51 pKa = 5.77 KK52 pKa = 10.61 KK53 pKa = 9.84
Molecular weight: 6.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.329
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.252
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.974
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.063
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3402
0
3402
1146857
25
3918
337.1
37.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.754 ± 0.047
0.805 ± 0.018
5.367 ± 0.029
6.236 ± 0.048
5.153 ± 0.035
6.387 ± 0.038
1.769 ± 0.021
7.674 ± 0.04
7.473 ± 0.056
9.185 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.239 ± 0.022
6.069 ± 0.05
3.592 ± 0.029
3.672 ± 0.024
3.525 ± 0.028
6.197 ± 0.033
6.331 ± 0.067
6.292 ± 0.037
1.015 ± 0.014
4.266 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here