Amino acid dipepetide frequency for Flavobacterium sp. CC-CTC003

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.725AlaAla: 4.725 ± 0.096	0.614AlaCys: 0.614 ± 0.041	3.54AlaAsp: 3.54 ± 0.056	3.861AlaGlu: 3.861 ± 0.075	3.514AlaPhe: 3.514 ± 0.057	4.266AlaGly: 4.266 ± 0.083	1.103AlaHis: 1.103 ± 0.038	5.743AlaIle: 5.743 ± 0.075	4.611AlaLys: 4.611 ± 0.078	6.741AlaLeu: 6.741 ± 0.083	1.594AlaMet: 1.594 ± 0.044	3.595AlaAsn: 3.595 ± 0.069	2.256AlaPro: 2.256 ± 0.066	2.698AlaGln: 2.698 ± 0.049	2.11AlaArg: 2.11 ± 0.043	4.144AlaSer: 4.144 ± 0.075	4.659AlaThr: 4.659 ± 0.107	4.588AlaVal: 4.588 ± 0.072	0.557AlaTrp: 0.557 ± 0.023	2.623AlaTyr: 2.623 ± 0.057	0.0AlaXaa: 0.0 ± 0.0
Cys	0.443CysAla: 0.443 ± 0.024	0.116CysCys: 0.116 ± 0.011	0.448CysAsp: 0.448 ± 0.023	0.472CysGlu: 0.472 ± 0.022	0.398CysPhe: 0.398 ± 0.019	0.656CysGly: 0.656 ± 0.038	0.2CysHis: 0.2 ± 0.015	0.6CysIle: 0.6 ± 0.025	0.464CysLys: 0.464 ± 0.019	0.692CysLeu: 0.692 ± 0.024	0.161CysMet: 0.161 ± 0.011	0.492CysAsn: 0.492 ± 0.027	0.398CysPro: 0.398 ± 0.026	0.25CysGln: 0.25 ± 0.018	0.255CysArg: 0.255 ± 0.015	0.626CysSer: 0.626 ± 0.033	0.535CysThr: 0.535 ± 0.028	0.421CysVal: 0.421 ± 0.022	0.071CysTrp: 0.071 ± 0.008	0.357CysTyr: 0.357 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.648AspAla: 3.648 ± 0.059	0.454AspCys: 0.454 ± 0.023	2.659AspAsp: 2.659 ± 0.064	3.33AspGlu: 3.33 ± 0.07	3.51AspPhe: 3.51 ± 0.058	3.595AspGly: 3.595 ± 0.081	0.974AspHis: 0.974 ± 0.028	4.007AspIle: 4.007 ± 0.059	4.127AspLys: 4.127 ± 0.072	4.82AspLeu: 4.82 ± 0.057	1.107AspMet: 1.107 ± 0.029	3.191AspAsn: 3.191 ± 0.073	1.798AspPro: 1.798 ± 0.044	1.634AspGln: 1.634 ± 0.042	1.981AspArg: 1.981 ± 0.042	3.001AspSer: 3.001 ± 0.059	2.955AspThr: 2.955 ± 0.057	3.292AspVal: 3.292 ± 0.071	0.701AspTrp: 0.701 ± 0.025	2.89AspTyr: 2.89 ± 0.048	0.0AspXaa: 0.0 ± 0.0
Glu	4.429GluAla: 4.429 ± 0.081	0.38GluCys: 0.38 ± 0.023	2.802GluAsp: 2.802 ± 0.057	4.15GluGlu: 4.15 ± 0.09	2.597GluPhe: 2.597 ± 0.052	3.37GluGly: 3.37 ± 0.059	1.145GluHis: 1.145 ± 0.036	5.206GluIle: 5.206 ± 0.08	6.124GluLys: 6.124 ± 0.094	5.771GluLeu: 5.771 ± 0.097	1.675GluMet: 1.675 ± 0.04	4.415GluAsn: 4.415 ± 0.074	1.576GluPro: 1.576 ± 0.041	2.428GluGln: 2.428 ± 0.045	2.473GluArg: 2.473 ± 0.056	3.131GluSer: 3.131 ± 0.053	3.774GluThr: 3.774 ± 0.057	3.892GluVal: 3.892 ± 0.061	0.635GluTrp: 0.635 ± 0.023	2.386GluTyr: 2.386 ± 0.041	0.0GluXaa: 0.0 ± 0.0
Phe	3.073PheAla: 3.073 ± 0.063	0.485PheCys: 0.485 ± 0.024	3.163PheAsp: 3.163 ± 0.056	3.33PheGlu: 3.33 ± 0.057	2.751PhePhe: 2.751 ± 0.064	3.51PheGly: 3.51 ± 0.067	0.879PheHis: 0.879 ± 0.026	3.61PheIle: 3.61 ± 0.071	3.371PheLys: 3.371 ± 0.059	4.688PheLeu: 4.688 ± 0.084	1.085PheMet: 1.085 ± 0.03	3.015PheAsn: 3.015 ± 0.057	1.847PhePro: 1.847 ± 0.043	1.708PheGln: 1.708 ± 0.043	1.869PheArg: 1.869 ± 0.037	3.967PheSer: 3.967 ± 0.071	3.334PheThr: 3.334 ± 0.065	2.988PheVal: 2.988 ± 0.059	0.555PheTrp: 0.555 ± 0.025	2.304PheTyr: 2.304 ± 0.054	0.0PheXaa: 0.0 ± 0.0
Gly	4.277GlyAla: 4.277 ± 0.074	0.643GlyCys: 0.643 ± 0.03	3.078GlyAsp: 3.078 ± 0.064	3.282GlyGlu: 3.282 ± 0.06	3.531GlyPhe: 3.531 ± 0.068	4.341GlyGly: 4.341 ± 0.114	1.128GlyHis: 1.128 ± 0.035	5.38GlyIle: 5.38 ± 0.083	4.94GlyLys: 4.94 ± 0.089	5.692GlyLeu: 5.692 ± 0.077	1.656GlyMet: 1.656 ± 0.043	3.931GlyAsn: 3.931 ± 0.089	1.354GlyPro: 1.354 ± 0.044	2.121GlyGln: 2.121 ± 0.047	2.016GlyArg: 2.016 ± 0.048	4.084GlySer: 4.084 ± 0.076	4.62GlyThr: 4.62 ± 0.133	4.064GlyVal: 4.064 ± 0.067	0.723GlyTrp: 0.723 ± 0.03	3.007GlyTyr: 3.007 ± 0.058	0.0GlyXaa: 0.0 ± 0.0
His	0.93HisAla: 0.93 ± 0.028	0.18HisCys: 0.18 ± 0.013	0.922HisAsp: 0.922 ± 0.025	0.974HisGlu: 0.974 ± 0.034	1.295HisPhe: 1.295 ± 0.04	0.989HisGly: 0.989 ± 0.036	0.5HisHis: 0.5 ± 0.025	1.377HisIle: 1.377 ± 0.039	1.182HisLys: 1.182 ± 0.036	1.664HisLeu: 1.664 ± 0.043	0.323HisMet: 0.323 ± 0.015	0.993HisAsn: 0.993 ± 0.034	0.834HisPro: 0.834 ± 0.027	0.776HisGln: 0.776 ± 0.026	0.662HisArg: 0.662 ± 0.024	1.072HisSer: 1.072 ± 0.028	0.996HisThr: 0.996 ± 0.03	0.848HisVal: 0.848 ± 0.028	0.201HisTrp: 0.201 ± 0.014	0.97HisTyr: 0.97 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.979IleAla: 5.979 ± 0.084	0.683IleCys: 0.683 ± 0.027	4.456IleAsp: 4.456 ± 0.072	4.971IleGlu: 4.971 ± 0.076	3.267IlePhe: 3.267 ± 0.067	5.083IleGly: 5.083 ± 0.078	1.398IleHis: 1.398 ± 0.037	5.462IleIle: 5.462 ± 0.085	5.402IleLys: 5.402 ± 0.075	6.672IleLeu: 6.672 ± 0.095	1.386IleMet: 1.386 ± 0.04	4.279IleAsn: 4.279 ± 0.068	3.408IlePro: 3.408 ± 0.052	2.87IleGln: 2.87 ± 0.048	3.07IleArg: 3.07 ± 0.059	4.991IleSer: 4.991 ± 0.075	5.026IleThr: 5.026 ± 0.089	4.923IleVal: 4.923 ± 0.08	0.701IleTrp: 0.701 ± 0.022	2.711IleTyr: 2.711 ± 0.053	0.0IleXaa: 0.0 ± 0.0
Lys	5.263LysAla: 5.263 ± 0.084	0.367LysCys: 0.367 ± 0.016	4.061LysAsp: 4.061 ± 0.073	5.739LysGlu: 5.739 ± 0.093	2.647LysPhe: 2.647 ± 0.05	4.408LysGly: 4.408 ± 0.074	1.229LysHis: 1.229 ± 0.037	6.077LysIle: 6.077 ± 0.088	6.537LysLys: 6.537 ± 0.107	6.151LysLeu: 6.151 ± 0.096	2.123LysMet: 2.123 ± 0.049	5.124LysAsn: 5.124 ± 0.087	2.498LysPro: 2.498 ± 0.046	2.761LysGln: 2.761 ± 0.053	2.666LysArg: 2.666 ± 0.059	4.098LysSer: 4.098 ± 0.073	4.741LysThr: 4.741 ± 0.073	4.625LysVal: 4.625 ± 0.072	0.764LysTrp: 0.764 ± 0.024	2.852LysTyr: 2.852 ± 0.055	0.0LysXaa: 0.0 ± 0.0
Leu	6.033LeuAla: 6.033 ± 0.087	0.68LeuCys: 0.68 ± 0.024	4.762LeuAsp: 4.762 ± 0.064	5.906LeuGlu: 5.906 ± 0.093	5.049LeuPhe: 5.049 ± 0.089	5.54LeuGly: 5.54 ± 0.084	1.687LeuHis: 1.687 ± 0.046	6.444LeuIle: 6.444 ± 0.096	7.203LeuLys: 7.203 ± 0.087	9.306LeuLeu: 9.306 ± 0.14	2.028LeuMet: 2.028 ± 0.047	5.267LeuAsn: 5.267 ± 0.08	3.691LeuPro: 3.691 ± 0.064	3.601LeuGln: 3.601 ± 0.068	3.204LeuArg: 3.204 ± 0.061	6.399LeuSer: 6.399 ± 0.075	5.477LeuThr: 5.477 ± 0.077	5.266LeuVal: 5.266 ± 0.077	0.806LeuTrp: 0.806 ± 0.03	3.501LeuTyr: 3.501 ± 0.059	0.0LeuXaa: 0.0 ± 0.0
Met	1.725MetAla: 1.725 ± 0.041	0.13MetCys: 0.13 ± 0.011	1.226MetAsp: 1.226 ± 0.034	1.502MetGlu: 1.502 ± 0.036	0.842MetPhe: 0.842 ± 0.032	1.447MetGly: 1.447 ± 0.043	0.394MetHis: 0.394 ± 0.019	1.576MetIle: 1.576 ± 0.038	2.111MetLys: 2.111 ± 0.045	1.974MetLeu: 1.974 ± 0.044	0.578MetMet: 0.578 ± 0.026	1.31MetAsn: 1.31 ± 0.036	0.857MetPro: 0.857 ± 0.026	0.848MetGln: 0.848 ± 0.028	0.891MetArg: 0.891 ± 0.03	1.369MetSer: 1.369 ± 0.033	1.285MetThr: 1.285 ± 0.035	1.424MetVal: 1.424 ± 0.039	0.158MetTrp: 0.158 ± 0.012	0.739MetTyr: 0.739 ± 0.03	0.0MetXaa: 0.0 ± 0.0
Asn	4.014AsnAla: 4.014 ± 0.074	0.554AsnCys: 0.554 ± 0.041	3.274AsnAsp: 3.274 ± 0.061	3.416AsnGlu: 3.416 ± 0.06	2.881AsnPhe: 2.881 ± 0.065	4.488AsnGly: 4.488 ± 0.104	1.072AsnHis: 1.072 ± 0.03	4.404AsnIle: 4.404 ± 0.056	3.931AsnLys: 3.931 ± 0.064	4.982AsnLeu: 4.982 ± 0.075	1.29AsnMet: 1.29 ± 0.035	3.775AsnAsn: 3.775 ± 0.085	3.129AsnPro: 3.129 ± 0.069	2.316AsnGln: 2.316 ± 0.051	2.366AsnArg: 2.366 ± 0.049	3.672AsnSer: 3.672 ± 0.068	3.924AsnThr: 3.924 ± 0.07	3.5AsnVal: 3.5 ± 0.071	0.786AsnTrp: 0.786 ± 0.029	2.918AsnTyr: 2.918 ± 0.062	0.0AsnXaa: 0.0 ± 0.0
Pro	2.308ProAla: 2.308 ± 0.062	0.229ProCys: 0.229 ± 0.015	2.268ProAsp: 2.268 ± 0.049	2.976ProGlu: 2.976 ± 0.052	1.995ProPhe: 1.995 ± 0.039	2.148ProGly: 2.148 ± 0.057	0.558ProHis: 0.558 ± 0.023	2.59ProIle: 2.59 ± 0.051	2.485ProLys: 2.485 ± 0.056	3.143ProLeu: 3.143 ± 0.052	0.776ProMet: 0.776 ± 0.024	2.31ProAsn: 2.31 ± 0.062	0.875ProPro: 0.875 ± 0.038	1.188ProGln: 1.188 ± 0.035	0.882ProArg: 0.882 ± 0.031	2.153ProSer: 2.153 ± 0.048	2.266ProThr: 2.266 ± 0.082	2.905ProVal: 2.905 ± 0.054	0.298ProTrp: 0.298 ± 0.016	1.563ProTyr: 1.563 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	2.213GlnAla: 2.213 ± 0.047	0.212GlnCys: 0.212 ± 0.014	1.699GlnAsp: 1.699 ± 0.039	2.343GlnGlu: 2.343 ± 0.048	1.779GlnPhe: 1.779 ± 0.042	1.774GlnGly: 1.774 ± 0.045	0.698GlnHis: 0.698 ± 0.025	2.662GlnIle: 2.662 ± 0.056	3.234GlnLys: 3.234 ± 0.061	3.838GlnLeu: 3.838 ± 0.059	0.927GlnMet: 0.927 ± 0.032	2.513GlnAsn: 2.513 ± 0.054	1.27GlnPro: 1.27 ± 0.042	1.761GlnGln: 1.761 ± 0.048	1.263GlnArg: 1.263 ± 0.036	2.253GlnSer: 2.253 ± 0.046	2.161GlnThr: 2.161 ± 0.045	2.103GlnVal: 2.103 ± 0.044	0.408GlnTrp: 0.408 ± 0.021	1.608GlnTyr: 1.608 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	1.919ArgAla: 1.919 ± 0.038	0.216ArgCys: 0.216 ± 0.014	1.745ArgAsp: 1.745 ± 0.042	2.278ArgGlu: 2.278 ± 0.063	2.122ArgPhe: 2.122 ± 0.051	1.772ArgGly: 1.772 ± 0.044	0.648ArgHis: 0.648 ± 0.023	3.129ArgIle: 3.129 ± 0.052	2.963ArgLys: 2.963 ± 0.062	3.462ArgLeu: 3.462 ± 0.055	1.009ArgMet: 1.009 ± 0.032	2.333ArgAsn: 2.333 ± 0.047	1.112ArgPro: 1.112 ± 0.03	1.275ArgGln: 1.275 ± 0.036	1.241ArgArg: 1.241 ± 0.039	1.966ArgSer: 1.966 ± 0.042	1.872ArgThr: 1.872 ± 0.041	2.064ArgVal: 2.064 ± 0.048	0.385ArgTrp: 0.385 ± 0.018	1.736ArgTyr: 1.736 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	3.893SerAla: 3.893 ± 0.06	0.696SerCys: 0.696 ± 0.029	3.606SerAsp: 3.606 ± 0.053	3.874SerGlu: 3.874 ± 0.06	3.609SerPhe: 3.609 ± 0.058	4.978SerGly: 4.978 ± 0.084	1.046SerHis: 1.046 ± 0.029	4.681SerIle: 4.681 ± 0.076	4.227SerLys: 4.227 ± 0.079	5.685SerLeu: 5.685 ± 0.078	1.202SerMet: 1.202 ± 0.037	3.537SerAsn: 3.537 ± 0.069	2.059SerPro: 2.059 ± 0.037	2.183SerGln: 2.183 ± 0.045	2.123SerArg: 2.123 ± 0.043	3.68SerSer: 3.68 ± 0.086	3.336SerThr: 3.336 ± 0.079	4.108SerVal: 4.108 ± 0.056	0.674SerTrp: 0.674 ± 0.025	2.769SerTyr: 2.769 ± 0.059	0.0SerXaa: 0.0 ± 0.0
Thr	4.828ThrAla: 4.828 ± 0.141	0.392ThrCys: 0.392 ± 0.023	3.401ThrAsp: 3.401 ± 0.057	3.535ThrGlu: 3.535 ± 0.061	3.227ThrPhe: 3.227 ± 0.061	4.586ThrGly: 4.586 ± 0.117	1.007ThrHis: 1.007 ± 0.031	5.307ThrIle: 5.307 ± 0.086	3.716ThrLys: 3.716 ± 0.059	5.871ThrLeu: 5.871 ± 0.073	1.038ThrMet: 1.038 ± 0.032	3.581ThrAsn: 3.581 ± 0.098	2.768ThrPro: 2.768 ± 0.065	2.162ThrGln: 2.162 ± 0.055	1.838ThrArg: 1.838 ± 0.04	3.707ThrSer: 3.707 ± 0.072	4.52ThrThr: 4.52 ± 0.131	4.695ThrVal: 4.695 ± 0.107	0.557ThrTrp: 0.557 ± 0.028	2.577ThrTyr: 2.577 ± 0.063	0.0ThrXaa: 0.0 ± 0.0
Val	4.653ValAla: 4.653 ± 0.088	0.541ValCys: 0.541 ± 0.026	3.394ValAsp: 3.394 ± 0.065	3.476ValGlu: 3.476 ± 0.063	3.4ValPhe: 3.4 ± 0.063	3.642ValGly: 3.642 ± 0.058	0.986ValHis: 0.986 ± 0.027	4.847ValIle: 4.847 ± 0.073	4.386ValLys: 4.386 ± 0.064	5.885ValLeu: 5.885 ± 0.074	1.348ValMet: 1.348 ± 0.036	3.628ValAsn: 3.628 ± 0.068	2.421ValPro: 2.421 ± 0.057	2.019ValGln: 2.019 ± 0.037	2.194ValArg: 2.194 ± 0.043	4.297ValSer: 4.297 ± 0.073	4.361ValThr: 4.361 ± 0.104	4.394ValVal: 4.394 ± 0.084	0.543ValTrp: 0.543 ± 0.023	2.503ValTyr: 2.503 ± 0.045	0.0ValXaa: 0.0 ± 0.0
Trp	0.621TrpAla: 0.621 ± 0.029	0.106TrpCys: 0.106 ± 0.01	0.596TrpAsp: 0.596 ± 0.022	0.611TrpGlu: 0.611 ± 0.026	0.528TrpPhe: 0.528 ± 0.024	0.608TrpGly: 0.608 ± 0.025	0.204TrpHis: 0.204 ± 0.012	0.744TrpIle: 0.744 ± 0.026	0.805TrpLys: 0.805 ± 0.033	0.962TrpLeu: 0.962 ± 0.032	0.301TrpMet: 0.301 ± 0.017	0.734TrpAsn: 0.734 ± 0.026	0.181TrpPro: 0.181 ± 0.015	0.395TrpGln: 0.395 ± 0.021	0.347TrpArg: 0.347 ± 0.019	0.63TrpSer: 0.63 ± 0.022	0.612TrpThr: 0.612 ± 0.029	0.58TrpVal: 0.58 ± 0.022	0.118TrpTrp: 0.118 ± 0.01	0.465TrpTyr: 0.465 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.568TyrAla: 2.568 ± 0.049	0.378TyrCys: 0.378 ± 0.018	2.575TyrAsp: 2.575 ± 0.058	2.332TyrGlu: 2.332 ± 0.048	2.59TyrPhe: 2.59 ± 0.056	2.666TyrGly: 2.666 ± 0.053	0.831TyrHis: 0.831 ± 0.028	2.89TyrIle: 2.89 ± 0.052	2.914TyrLys: 2.914 ± 0.058	3.851TyrLeu: 3.851 ± 0.056	0.78TyrMet: 0.78 ± 0.023	2.669TyrAsn: 2.669 ± 0.066	1.587TyrPro: 1.587 ± 0.042	1.726TyrGln: 1.726 ± 0.043	1.796TyrArg: 1.796 ± 0.045	2.724TyrSer: 2.724 ± 0.054	2.862TyrThr: 2.862 ± 0.081	2.236TyrVal: 2.236 ± 0.053	0.507TyrTrp: 0.507 ± 0.02	2.178TyrTyr: 2.178 ± 0.056	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3402 proteins (1146857 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---