Ktedonobacter racemifer DSM 44963
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11211 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D6TY45|D6TY45_9CHLR Uncharacterized protein OS=Ktedonobacter racemifer DSM 44963 OX=485913 GN=Krac_6180 PE=4 SV=1
MM1 pKa = 7.27 QIAALQEE8 pKa = 4.29 GSASDD13 pKa = 3.56 EE14 pKa = 3.94 FAIYY18 pKa = 9.91 SWPEE22 pKa = 3.39 RR23 pKa = 11.84 QLLQRR28 pKa = 11.84 YY29 pKa = 7.96 PVMPALDD36 pKa = 4.11 GWPVDD41 pKa = 4.06 MVLSPQDD48 pKa = 3.65 NLALCMFANEE58 pKa = 4.79 DD59 pKa = 3.65 DD60 pKa = 4.12 VSFEE64 pKa = 4.62 FIDD67 pKa = 3.41 ITSQGVSQDD76 pKa = 2.85 LHH78 pKa = 7.67 LYY80 pKa = 9.97 KK81 pKa = 10.82 GAII84 pKa = 3.65
Molecular weight: 9.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.753
IPC2_protein 3.795
IPC_protein 3.719
Toseland 3.516
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.681
Rodwell 3.554
Grimsley 3.439
Solomon 3.694
Lehninger 3.643
Nozaki 3.859
DTASelect 4.062
Thurlkill 3.592
EMBOSS 3.694
Sillero 3.846
Patrickios 1.875
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.761
Protein with the highest isoelectric point:
>tr|D6TXN5|D6TXN5_9CHLR Uncharacterized protein OS=Ktedonobacter racemifer DSM 44963 OX=485913 GN=Krac_3999 PE=4 SV=1
MM1 pKa = 7.15 FVLSRR6 pKa = 11.84 RR7 pKa = 11.84 QRR9 pKa = 11.84 RR10 pKa = 11.84 VMMRR14 pKa = 11.84 FLQALAVQGVVLVVRR29 pKa = 11.84 HH30 pKa = 5.02 WLHH33 pKa = 5.28 WW34 pKa = 3.94
Molecular weight: 4.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.43
IPC2_protein 10.921
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.193
Grimsley 12.735
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 11.974
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.149
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11211
0
11211
3395074
30
3346
302.8
33.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.38 ± 0.025
1.117 ± 0.008
4.511 ± 0.016
6.099 ± 0.029
3.604 ± 0.015
7.039 ± 0.02
2.743 ± 0.011
5.322 ± 0.019
3.569 ± 0.023
11.154 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.209 ± 0.009
3.032 ± 0.016
5.102 ± 0.019
5.205 ± 0.02
6.618 ± 0.023
6.126 ± 0.021
5.889 ± 0.019
6.668 ± 0.019
1.594 ± 0.011
3.019 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here