Paenibacillus sp. 597

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7604 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A561Q655|A0A561Q655_9BACL 2-dehydro-3-deoxygluconokinase OS=Paenibacillus sp. 597 OX=2587047 GN=FHW43_104103 PE=4 SV=1
MM1 pKa = 7.93ADD3 pKa = 2.88MMLRR7 pKa = 11.84PDD9 pKa = 4.15LKK11 pKa = 9.01TAGGEE16 pKa = 4.3VCDD19 pKa = 3.76IMYY22 pKa = 10.58NGEE25 pKa = 4.21FVGTLTLVYY34 pKa = 10.26RR35 pKa = 11.84EE36 pKa = 3.96SDD38 pKa = 3.24RR39 pKa = 11.84LKK41 pKa = 11.19GSIHH45 pKa = 7.29LEE47 pKa = 3.93RR48 pKa = 11.84QSIAQEE54 pKa = 4.22DD55 pKa = 4.34KK56 pKa = 8.73EE57 pKa = 4.48SVYY60 pKa = 11.32AFVQSYY66 pKa = 8.29IQSVIDD72 pKa = 3.51ALAIEE77 pKa = 4.48EE78 pKa = 4.69CEE80 pKa = 4.36VVANYY85 pKa = 10.66SDD87 pKa = 4.01YY88 pKa = 11.7DD89 pKa = 3.57FVIATDD95 pKa = 3.73HH96 pKa = 7.17AIGPVLGEE104 pKa = 4.03EE105 pKa = 4.99DD106 pKa = 4.56DD107 pKa = 5.42RR108 pKa = 11.84SDD110 pKa = 4.14PYY112 pKa = 11.78YY113 pKa = 11.04LDD115 pKa = 6.01DD116 pKa = 5.04EE117 pKa = 4.82DD118 pKa = 6.38ADD120 pKa = 4.27YY121 pKa = 11.39EE122 pKa = 4.46WVNDD126 pKa = 3.74DD127 pKa = 4.95EE128 pKa = 5.84IRR130 pKa = 11.84FDD132 pKa = 6.6DD133 pKa = 3.9IDD135 pKa = 3.75GTNGPEE141 pKa = 3.85SLYY144 pKa = 10.32MIEE147 pKa = 5.07QRR149 pKa = 11.84RR150 pKa = 11.84QQLGYY155 pKa = 9.09EE156 pKa = 3.96LVIVGEE162 pKa = 3.99YY163 pKa = 10.26RR164 pKa = 11.84NLVEE168 pKa = 3.58YY169 pKa = 10.23HH170 pKa = 6.69VYY172 pKa = 10.54DD173 pKa = 4.02KK174 pKa = 11.14EE175 pKa = 4.55SEE177 pKa = 4.31LVAEE181 pKa = 5.21AVTHH185 pKa = 5.85MYY187 pKa = 10.47GSEE190 pKa = 4.21VNGEE194 pKa = 4.23VTWRR198 pKa = 11.84FDD200 pKa = 3.63PFDD203 pKa = 3.96EE204 pKa = 4.68EE205 pKa = 4.06MDD207 pKa = 3.9LVTEE211 pKa = 5.25LIVADD216 pKa = 4.0YY217 pKa = 11.45DD218 pKa = 3.79EE219 pKa = 6.38DD220 pKa = 3.82EE221 pKa = 4.33TDD223 pKa = 3.21TFIFNMIFDD232 pKa = 4.52GEE234 pKa = 4.51VIDD237 pKa = 4.33TVEE240 pKa = 4.11LTHH243 pKa = 7.34IDD245 pKa = 3.85LLEE248 pKa = 4.23LVDD251 pKa = 5.64DD252 pKa = 4.05IRR254 pKa = 11.84EE255 pKa = 4.01EE256 pKa = 4.35SLMPPDD262 pKa = 4.6PEE264 pKa = 6.62DD265 pKa = 3.45YY266 pKa = 10.55TITLARR272 pKa = 11.84DD273 pKa = 3.6DD274 pKa = 5.11GDD276 pKa = 3.53TLTYY280 pKa = 10.34EE281 pKa = 5.21IYY283 pKa = 8.77QQSYY287 pKa = 8.98GGLPIGTATVDD298 pKa = 2.86ISSRR302 pKa = 11.84QLTGFIDD309 pKa = 3.9FRR311 pKa = 11.84EE312 pKa = 4.54PGDD315 pKa = 3.53SDD317 pKa = 4.02DD318 pKa = 5.17RR319 pKa = 11.84EE320 pKa = 4.47LIAALLLDD328 pKa = 4.65EE329 pKa = 5.37LDD331 pKa = 4.27KK332 pKa = 11.75EE333 pKa = 4.21NDD335 pKa = 3.45YY336 pKa = 10.02EE337 pKa = 4.37TFNVSMLYY345 pKa = 10.0RR346 pKa = 11.84NKK348 pKa = 10.64LIDD351 pKa = 4.89EE352 pKa = 4.25IWFEE356 pKa = 4.17TDD358 pKa = 2.74QLHH361 pKa = 6.37

Molecular weight: 41.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A561PPL5|A0A561PPL5_9BACL Glycosyl transferase family 2 OS=Paenibacillus sp. 597 OX=2587047 GN=FHW43_1096 PE=4 SV=1
MM1 pKa = 7.61GPTFNPNTRR10 pKa = 11.84KK11 pKa = 9.86RR12 pKa = 11.84KK13 pKa = 8.79KK14 pKa = 9.13NHH16 pKa = 5.06GFRR19 pKa = 11.84KK20 pKa = 9.98RR21 pKa = 11.84MSTKK25 pKa = 10.0NGRR28 pKa = 11.84KK29 pKa = 8.8VLQARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.45GRR39 pKa = 11.84KK40 pKa = 8.72ILSAA44 pKa = 4.02

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7604

0

7604

2559972

29

5752

336.7

37.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.945 ± 0.031

0.733 ± 0.009

5.091 ± 0.02

6.282 ± 0.036

4.275 ± 0.02

7.099 ± 0.031

2.165 ± 0.015

6.959 ± 0.028

5.638 ± 0.026

10.011 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.87 ± 0.015

4.111 ± 0.028

4.006 ± 0.018

4.061 ± 0.021

4.471 ± 0.026

6.592 ± 0.026

5.74 ± 0.031

7.036 ± 0.026

1.334 ± 0.011

3.581 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski