Penaeus vannamei (Whiteleg shrimp) (Litopenaeus vannamei)
Average proteome isoelectric point is 7.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 25399 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A423TD78|A0A423TD78_PENVA Uncharacterized protein OS=Penaeus vannamei OX=6689 GN=C7M84_007163 PE=3 SV=1
MM1 pKa = 7.28 FLSSHH6 pKa = 7.06 LCPLSYY12 pKa = 10.96 SSLSLFLDD20 pKa = 3.96 SLSSPLSCLALPWLEE35 pKa = 3.82 ITYY38 pKa = 7.52 EE39 pKa = 4.08 TTDD42 pKa = 3.08 GKK44 pKa = 11.2 KK45 pKa = 8.81 FTVQGKK51 pKa = 9.77 EE52 pKa = 3.69 GDD54 pKa = 3.68 NLLDD58 pKa = 3.31 IVINNDD64 pKa = 2.46 VDD66 pKa = 4.62 LDD68 pKa = 4.04 GFGACEE74 pKa = 3.88 GTLACSTCHH83 pKa = 7.38 LIFKK87 pKa = 10.57 KK88 pKa = 10.58 EE89 pKa = 3.86 DD90 pKa = 3.24 FDD92 pKa = 6.26 RR93 pKa = 11.84 IEE95 pKa = 4.32 EE96 pKa = 4.27 PSTDD100 pKa = 3.85 EE101 pKa = 4.17 EE102 pKa = 6.21 LDD104 pKa = 3.8 MLDD107 pKa = 3.84 LAYY110 pKa = 10.69 GLTDD114 pKa = 3.18 TT115 pKa = 5.38
Molecular weight: 12.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.859
IPC_protein 3.834
Toseland 3.617
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.656
Grimsley 3.541
Solomon 3.808
Lehninger 3.77
Nozaki 3.948
DTASelect 4.164
Thurlkill 3.681
EMBOSS 3.77
Sillero 3.948
Patrickios 0.947
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.853
Protein with the highest isoelectric point:
>tr|A0A423U557|A0A423U557_PENVA Crustacyanin subunit A OS=Penaeus vannamei OX=6689 GN=C7M84_023007 PE=3 SV=1
MM1 pKa = 7.62 VDD3 pKa = 3.32 EE4 pKa = 4.64 VKK6 pKa = 10.39 SARR9 pKa = 11.84 RR10 pKa = 11.84 KK11 pKa = 9.71 VLSLHH16 pKa = 6.79 LSLHH20 pKa = 6.47 LSASPLSPLASPLASPPRR38 pKa = 11.84 PLRR41 pKa = 11.84 FTLATSPSLNLSLHH55 pKa = 6.39 LSLFTSRR62 pKa = 11.84 LHH64 pKa = 6.66 LSLPSRR70 pKa = 11.84 SPLRR74 pKa = 11.84 FTSRR78 pKa = 11.84 SLASTLSALDD88 pKa = 3.44 TSRR91 pKa = 11.84 LTFLALSPLASSLQTSRR108 pKa = 11.84 FHH110 pKa = 6.65 LRR112 pKa = 11.84 SSPSPLASPLVHH124 pKa = 6.9 LSLHH128 pKa = 6.42 LSFTSRR134 pKa = 11.84 FTSRR138 pKa = 11.84 FTSRR142 pKa = 11.84 FTSRR146 pKa = 11.84 TSRR149 pKa = 11.84 FNLSRR154 pKa = 11.84 SALFASPLAQPLASSFTSRR173 pKa = 11.84 QLRR176 pKa = 11.84 SPLASPLSFTSRR188 pKa = 11.84 LLSLHH193 pKa = 6.69 LSLHH197 pKa = 6.16 LVASHH202 pKa = 7.19 LSLTSRR208 pKa = 11.84 FTSRR212 pKa = 11.84 PPSRR216 pKa = 11.84 FISLHH221 pKa = 5.19 PSRR224 pKa = 11.84 FNLSSPLASPLVSPLASPLVHH245 pKa = 6.82 LLASRR250 pKa = 11.84 SRR252 pKa = 11.84 FTSRR256 pKa = 11.84 FTSPLVSTSSLHH268 pKa = 6.84 LSLHH272 pKa = 6.46 LSLSRR277 pKa = 11.84 PLASPHH283 pKa = 6.23 LRR285 pKa = 11.84 FTFVRR290 pKa = 11.84 LSLSPLASPLLQHH303 pKa = 6.66 SRR305 pKa = 11.84 FTSRR309 pKa = 11.84 FTSRR313 pKa = 11.84 FISSRR318 pKa = 11.84 SSLHH322 pKa = 6.42 LSLHH326 pKa = 6.39 LSLHH330 pKa = 5.96 LASPLASPLASPLASPLASPLASPLASPLASPLASPLASPLASPLALAFTSRR382 pKa = 11.84 ASPPLTSRR390 pKa = 11.84 FTSRR394 pKa = 11.84 FTSLALTSRR403 pKa = 11.84 FTSRR407 pKa = 11.84 FTSRR411 pKa = 11.84 SPLSSPLSPLASPLASPSRR430 pKa = 11.84 FTSRR434 pKa = 11.84 FTSLHH439 pKa = 6.16 LSLTSRR445 pKa = 11.84 FTSRR449 pKa = 11.84 FTSRR453 pKa = 11.84 FTLAFTSRR461 pKa = 11.84 SSPLFLASPLAFSLSLRR478 pKa = 11.84 LSLHH482 pKa = 6.49 LSLHH486 pKa = 6.37 LAPLASTSASRR497 pKa = 11.84 LTLTSRR503 pKa = 11.84 FTLASPSSLLASPLASPLVSPLASLSLHH531 pKa = 5.6 FTLSLHH537 pKa = 6.91 LSLHH541 pKa = 6.03 LCFTSLHH548 pKa = 6.2 LSLHH552 pKa = 6.3 LSLHH556 pKa = 6.37 LSLHH560 pKa = 6.67 LSPHH564 pKa = 6.25 SPLASPLARR573 pKa = 11.84 SRR575 pKa = 11.84 FASRR579 pKa = 11.84 SPLRR583 pKa = 11.84 SSRR586 pKa = 11.84 FNLSPQLSFTSRR598 pKa = 11.84 FTSRR602 pKa = 11.84 SPSSPLASPLARR614 pKa = 11.84 LSLLSLTSRR623 pKa = 11.84 FHH625 pKa = 7.51 LSLLTSRR632 pKa = 11.84 FTLASPLLHH641 pKa = 7.18 LSLTSLAFTSRR652 pKa = 11.84 FHH654 pKa = 6.91 PLGFTSRR661 pKa = 11.84 FTLSLSPLASSSSLRR676 pKa = 11.84 SPSPLVSPLVSPLASPSLSPLVHH699 pKa = 6.83 LSLTSRR705 pKa = 11.84 FPSLASTSRR714 pKa = 11.84 FTSRR718 pKa = 11.84 FTSPASPLVSPLASPLASPLAFSRR742 pKa = 11.84 FTSRR746 pKa = 11.84 FTSRR750 pKa = 11.84 FLALFSSFPAGG761 pKa = 3.22
Molecular weight: 81.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.33
IPC_protein 12.939
Toseland 13.1
ProMoST 13.598
Dawson 13.1
Bjellqvist 13.1
Wikipedia 13.583
Rodwell 12.618
Grimsley 13.144
Solomon 13.598
Lehninger 13.495
Nozaki 13.1
DTASelect 13.1
Thurlkill 13.1
EMBOSS 13.598
Sillero 13.1
Patrickios 12.325
IPC_peptide 13.598
IPC2_peptide 12.588
IPC2.peptide.svr19 9.269
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
25399
0
25399
13085305
52
11752
515.2
56.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.592 ± 0.025
1.915 ± 0.012
4.093 ± 0.02
5.183 ± 0.035
4.316 ± 0.029
5.657 ± 0.029
3.201 ± 0.021
3.736 ± 0.016
3.929 ± 0.024
10.781 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.685 ± 0.009
3.055 ± 0.016
10.108 ± 0.064
3.702 ± 0.021
6.188 ± 0.028
10.942 ± 0.046
6.016 ± 0.033
5.499 ± 0.023
1.039 ± 0.007
2.357 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here