Synechococcus phage S-SBP1
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6M4ELK6|A0A6M4ELK6_9CAUD Single stranded DNA-binding protein OS=Synechococcus phage S-SBP1 OX=2735125 GN=SSBP1_gp12 PE=4 SV=1
MM1 pKa = 7.2 YY2 pKa = 10.07 NVSPMFDD9 pKa = 3.11 ALAEE13 pKa = 4.3 RR14 pKa = 11.84 FDD16 pKa = 6.41 DD17 pKa = 3.76 MDD19 pKa = 3.42 EE20 pKa = 4.74 CKK22 pKa = 10.57 DD23 pKa = 3.53 VAEE26 pKa = 4.47 YY27 pKa = 11.0 GCAAGVSGFIYY38 pKa = 10.33 SSEE41 pKa = 3.76 LAEE44 pKa = 5.19 FFDD47 pKa = 4.33 KK48 pKa = 11.63 YY49 pKa = 10.62 EE50 pKa = 4.63 DD51 pKa = 4.27 EE52 pKa = 5.96 IEE54 pKa = 5.38 DD55 pKa = 3.76 EE56 pKa = 4.34 LDD58 pKa = 3.52 TYY60 pKa = 9.81 GLKK63 pKa = 9.14 YY64 pKa = 9.22 TDD66 pKa = 4.91 LVDD69 pKa = 3.35 TSEE72 pKa = 4.67 FYY74 pKa = 10.87 TMQEE78 pKa = 4.4 CKK80 pKa = 10.2 EE81 pKa = 4.03 KK82 pKa = 10.83 AVWCIVEE89 pKa = 4.53 MYY91 pKa = 9.92 CHH93 pKa = 6.09 NRR95 pKa = 11.84 VDD97 pKa = 3.71 AACAVAA103 pKa = 5.16
Molecular weight: 11.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.864
IPC2_protein 3.846
IPC_protein 3.808
Toseland 3.605
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.63
Grimsley 3.516
Solomon 3.77
Lehninger 3.719
Nozaki 3.897
DTASelect 4.075
Thurlkill 3.656
EMBOSS 3.706
Sillero 3.923
Patrickios 0.172
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.822
Protein with the highest isoelectric point:
>tr|A0A6M4EMI7|A0A6M4EMI7_9CAUD Uncharacterized protein OS=Synechococcus phage S-SBP1 OX=2735125 GN=SSBP1_gp46 PE=4 SV=1
MM1 pKa = 7.82 TYY3 pKa = 10.1 RR4 pKa = 11.84 PHH6 pKa = 7.41 RR7 pKa = 11.84 RR8 pKa = 11.84 TLTSTIKK15 pKa = 10.51 VGKK18 pKa = 8.79 HH19 pKa = 3.96 LCKK22 pKa = 10.72 VFIKK26 pKa = 10.04 PWDD29 pKa = 3.7 KK30 pKa = 10.24 MPSGAVVWKK39 pKa = 10.59 VGFGVGKK46 pKa = 9.62 SRR48 pKa = 11.84 RR49 pKa = 11.84 QINDD53 pKa = 2.93 WYY55 pKa = 10.62 HH56 pKa = 5.43 VKK58 pKa = 10.27 QNRR61 pKa = 11.84 RR62 pKa = 11.84 RR63 pKa = 11.84 RR64 pKa = 11.84 SLHH67 pKa = 4.65 KK68 pKa = 10.36 HH69 pKa = 3.44 MTGTEE74 pKa = 4.16 GFKK77 pKa = 10.33 TIPRR81 pKa = 11.84 GFNEE85 pKa = 3.85 VLRR88 pKa = 11.84 LRR90 pKa = 11.84 WLIPAGDD97 pKa = 4.38 TIFIDD102 pKa = 4.11 CTSADD107 pKa = 4.36 PEE109 pKa = 4.5 KK110 pKa = 10.45 QWKK113 pKa = 7.45 TFSRR117 pKa = 11.84 WRR119 pKa = 11.84 RR120 pKa = 11.84 WHH122 pKa = 6.71 PDD124 pKa = 2.24 WFVNEE129 pKa = 4.25 HH130 pKa = 6.03 LKK132 pKa = 10.37 EE133 pKa = 4.5 FYY135 pKa = 7.55 WTRR138 pKa = 11.84 PP139 pKa = 3.32
Molecular weight: 16.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.316
IPC2_protein 9.838
IPC_protein 10.701
Toseland 11.067
ProMoST 10.847
Dawson 11.111
Bjellqvist 10.847
Wikipedia 11.345
Rodwell 11.359
Grimsley 11.14
Solomon 11.301
Lehninger 11.272
Nozaki 11.038
DTASelect 10.833
Thurlkill 11.038
EMBOSS 11.477
Sillero 11.052
Patrickios 11.082
IPC_peptide 11.316
IPC2_peptide 9.853
IPC2.peptide.svr19 8.613
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
14088
44
1325
256.1
28.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.008 ± 0.35
0.852 ± 0.157
6.708 ± 0.372
5.984 ± 0.434
3.677 ± 0.19
7.432 ± 0.529
1.711 ± 0.211
5.508 ± 0.25
5.402 ± 0.332
7.765 ± 0.324
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.655 ± 0.213
5.132 ± 0.299
3.996 ± 0.165
4.387 ± 0.367
4.891 ± 0.349
6.807 ± 0.348
7.269 ± 0.584
6.112 ± 0.273
1.249 ± 0.136
3.457 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here