Streptomyces sp. NA04227

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6361 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7H8JWY2|A0A7H8JWY2_9ACTN GYD domain-containing protein OS=Streptomyces sp. NA04227 OX=2742136 GN=HUT18_24440 PE=4 SV=1
MM1 pKa = 7.21SRR3 pKa = 11.84SARR6 pKa = 11.84TSRR9 pKa = 11.84SLGLLAASALIALPVGVGLAALPASAAEE37 pKa = 4.13PTATAVADD45 pKa = 4.28DD46 pKa = 4.81GGHH49 pKa = 5.63SVTYY53 pKa = 9.03TAGAQQYY60 pKa = 8.91NEE62 pKa = 4.1VVITEE67 pKa = 4.13AKK69 pKa = 8.82GTEE72 pKa = 3.93FTIDD76 pKa = 3.31DD77 pKa = 4.52TVPITAGTGCVHH89 pKa = 7.44PDD91 pKa = 2.79KK92 pKa = 10.85TDD94 pKa = 3.25QTKK97 pKa = 9.44VVCTLTEE104 pKa = 4.4FGDD107 pKa = 3.67YY108 pKa = 8.62WVRR111 pKa = 11.84IHH113 pKa = 7.56VDD115 pKa = 2.74LGDD118 pKa = 4.08YY119 pKa = 10.56KK120 pKa = 11.41DD121 pKa = 4.25EE122 pKa = 5.08LSMHH126 pKa = 7.09AGNEE130 pKa = 4.07NVIHH134 pKa = 6.45GGSGNDD140 pKa = 3.39TLTGDD145 pKa = 4.44GNDD148 pKa = 3.72MLYY151 pKa = 11.04GDD153 pKa = 5.64DD154 pKa = 5.69GDD156 pKa = 4.23DD157 pKa = 3.59TLTGGTQLGGNGADD171 pKa = 4.24LLSAPNYY178 pKa = 10.1SADD181 pKa = 3.33GGYY184 pKa = 11.31GNDD187 pKa = 3.71TLVGTDD193 pKa = 5.01DD194 pKa = 5.49ADD196 pKa = 3.77TLYY199 pKa = 11.04GGPDD203 pKa = 3.25HH204 pKa = 7.73DD205 pKa = 4.43MLIGRR210 pKa = 11.84AGDD213 pKa = 3.46DD214 pKa = 3.3VLYY217 pKa = 10.97GNSGNDD223 pKa = 3.44TIYY226 pKa = 11.17GGGGNDD232 pKa = 4.24TISGGPGTNEE242 pKa = 3.08IHH244 pKa = 5.78QQ245 pKa = 3.98

Molecular weight: 24.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7H8JVY9|A0A7H8JVY9_9ACTN Type II secretion system F family protein OS=Streptomyces sp. NA04227 OX=2742136 GN=HUT18_20980 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.36GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6361

0

6361

2163193

19

4938

340.1

36.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.35 ± 0.042

0.809 ± 0.008

5.966 ± 0.026

6.182 ± 0.03

2.705 ± 0.016

9.575 ± 0.034

2.329 ± 0.014

2.983 ± 0.019

2.303 ± 0.029

10.306 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.67 ± 0.012

1.799 ± 0.018

6.139 ± 0.03

2.842 ± 0.018

8.095 ± 0.035

5.265 ± 0.023

6.001 ± 0.022

8.142 ± 0.034

1.486 ± 0.013

2.054 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski