Amino acid dipepetide frequency for Streptomyces sp. NA04227

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.708AlaAla: 20.708 ± 0.17
1.05AlaCys: 1.05 ± 0.022
8.022AlaAsp: 8.022 ± 0.068
9.77AlaGlu: 9.77 ± 0.103
3.388AlaPhe: 3.388 ± 0.043
12.298AlaGly: 12.298 ± 0.091
3.061AlaHis: 3.061 ± 0.045
3.051AlaIle: 3.051 ± 0.041
3.07AlaLys: 3.07 ± 0.06
14.547AlaLeu: 14.547 ± 0.127
2.353AlaMet: 2.353 ± 0.035
1.933AlaAsn: 1.933 ± 0.03
7.367AlaPro: 7.367 ± 0.083
3.982AlaGln: 3.982 ± 0.053
10.304AlaArg: 10.304 ± 0.09
5.934AlaSer: 5.934 ± 0.062
6.913AlaThr: 6.913 ± 0.054
11.416AlaVal: 11.416 ± 0.088
1.746AlaTrp: 1.746 ± 0.026
2.585AlaTyr: 2.585 ± 0.037
0.0AlaXaa: 0.0 ± 0.0
Cys
1.131CysAla: 1.131 ± 0.024
0.104CysCys: 0.104 ± 0.008
0.478CysAsp: 0.478 ± 0.014
0.451CysGlu: 0.451 ± 0.014
0.223CysPhe: 0.223 ± 0.01
0.982CysGly: 0.982 ± 0.023
0.183CysHis: 0.183 ± 0.01
0.151CysIle: 0.151 ± 0.009
0.131CysLys: 0.131 ± 0.008
0.78CysLeu: 0.78 ± 0.019
0.132CysMet: 0.132 ± 0.009
0.142CysAsn: 0.142 ± 0.008
0.513CysPro: 0.513 ± 0.019
0.167CysGln: 0.167 ± 0.009
0.625CysArg: 0.625 ± 0.019
0.432CysSer: 0.432 ± 0.014
0.521CysThr: 0.521 ± 0.014
0.664CysVal: 0.664 ± 0.019
0.131CysTrp: 0.131 ± 0.007
0.149CysTyr: 0.149 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.216AspAla: 7.216 ± 0.053
0.484AspCys: 0.484 ± 0.016
3.398AspAsp: 3.398 ± 0.046
4.214AspGlu: 4.214 ± 0.05
1.728AspPhe: 1.728 ± 0.03
6.402AspGly: 6.402 ± 0.068
1.343AspHis: 1.343 ± 0.024
1.874AspIle: 1.874 ± 0.033
1.428AspLys: 1.428 ± 0.034
6.022AspLeu: 6.022 ± 0.058
0.819AspMet: 0.819 ± 0.019
1.049AspAsn: 1.049 ± 0.025
4.493AspPro: 4.493 ± 0.05
1.507AspGln: 1.507 ± 0.031
4.903AspArg: 4.903 ± 0.057
2.941AspSer: 2.941 ± 0.043
3.176AspThr: 3.176 ± 0.042
4.384AspVal: 4.384 ± 0.044
1.125AspTrp: 1.125 ± 0.024
1.153AspTyr: 1.153 ± 0.025
0.0AspXaa: 0.0 ± 0.0
Glu
7.844GluAla: 7.844 ± 0.087
0.409GluCys: 0.409 ± 0.016
3.265GluAsp: 3.265 ± 0.04
3.95GluGlu: 3.95 ± 0.067
1.666GluPhe: 1.666 ± 0.029
5.051GluGly: 5.051 ± 0.056
1.54GluHis: 1.54 ± 0.026
2.352GluIle: 2.352 ± 0.037
1.558GluLys: 1.558 ± 0.042
7.407GluLeu: 7.407 ± 0.08
0.889GluMet: 0.889 ± 0.02
1.17GluAsn: 1.17 ± 0.024
3.697GluPro: 3.697 ± 0.048
2.511GluGln: 2.511 ± 0.043
5.777GluArg: 5.777 ± 0.068
3.093GluSer: 3.093 ± 0.038
2.893GluThr: 2.893 ± 0.041
4.83GluVal: 4.83 ± 0.044
0.742GluTrp: 0.742 ± 0.017
1.173GluTyr: 1.173 ± 0.024
0.0GluXaa: 0.0 ± 0.0
Phe
3.701PheAla: 3.701 ± 0.038
0.267PheCys: 0.267 ± 0.011
1.937PheAsp: 1.937 ± 0.032
1.507PheGlu: 1.507 ± 0.027
0.815PhePhe: 0.815 ± 0.021
3.006PheGly: 3.006 ± 0.036
0.626PheHis: 0.626 ± 0.017
0.651PheIle: 0.651 ± 0.017
0.511PheLys: 0.511 ± 0.017
2.582PheLeu: 2.582 ± 0.038
0.374PheMet: 0.374 ± 0.015
0.546PheAsn: 0.546 ± 0.018
1.376PhePro: 1.376 ± 0.025
0.658PheGln: 0.658 ± 0.017
1.874PheArg: 1.874 ± 0.028
1.421PheSer: 1.421 ± 0.027
2.002PheThr: 2.002 ± 0.031
2.243PheVal: 2.243 ± 0.031
0.406PheTrp: 0.406 ± 0.014
0.553PheTyr: 0.553 ± 0.018
0.0PheXaa: 0.0 ± 0.0
Gly
11.388GlyAla: 11.388 ± 0.09
0.837GlyCys: 0.837 ± 0.019
5.202GlyAsp: 5.202 ± 0.055
5.883GlyGlu: 5.883 ± 0.063
2.767GlyPhe: 2.767 ± 0.035
9.17GlyGly: 9.17 ± 0.117
2.374GlyHis: 2.374 ± 0.036
3.317GlyIle: 3.317 ± 0.042
2.732GlyLys: 2.732 ± 0.053
9.09GlyLeu: 9.09 ± 0.088
1.936GlyMet: 1.936 ± 0.031
1.818GlyAsn: 1.818 ± 0.036
5.411GlyPro: 5.411 ± 0.082
2.854GlyGln: 2.854 ± 0.055
7.802GlyArg: 7.802 ± 0.068
5.735GlySer: 5.735 ± 0.063
6.484GlyThr: 6.484 ± 0.064
7.098GlyVal: 7.098 ± 0.069
1.619GlyTrp: 1.619 ± 0.027
2.237GlyTyr: 2.237 ± 0.039
0.0GlyXaa: 0.0 ± 0.0
His
2.649HisAla: 2.649 ± 0.04
0.235HisCys: 0.235 ± 0.01
1.378HisAsp: 1.378 ± 0.025
1.375HisGlu: 1.375 ± 0.025
0.659HisPhe: 0.659 ± 0.016
2.444HisGly: 2.444 ± 0.041
0.686HisHis: 0.686 ± 0.02
0.693HisIle: 0.693 ± 0.019
0.39HisLys: 0.39 ± 0.015
2.463HisLeu: 2.463 ± 0.04
0.326HisMet: 0.326 ± 0.012
0.393HisAsn: 0.393 ± 0.015
1.783HisPro: 1.783 ± 0.033
0.638HisGln: 0.638 ± 0.016
2.175HisArg: 2.175 ± 0.038
1.152HisSer: 1.152 ± 0.023
1.378HisThr: 1.378 ± 0.025
1.612HisVal: 1.612 ± 0.025
0.382HisTrp: 0.382 ± 0.014
0.478HisTyr: 0.478 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
4.398IleAla: 4.398 ± 0.052
0.3IleCys: 0.3 ± 0.011
2.166IleAsp: 2.166 ± 0.035
1.985IleGlu: 1.985 ± 0.034
0.669IlePhe: 0.669 ± 0.018
3.322IleGly: 3.322 ± 0.049
0.575IleHis: 0.575 ± 0.018
0.773IleIle: 0.773 ± 0.023
0.781IleLys: 0.781 ± 0.022
2.308IleLeu: 2.308 ± 0.034
0.404IleMet: 0.404 ± 0.014
0.68IleAsn: 0.68 ± 0.018
1.65IlePro: 1.65 ± 0.026
0.696IleGln: 0.696 ± 0.019
2.166IleArg: 2.166 ± 0.034
1.6IleSer: 1.6 ± 0.029
2.019IleThr: 2.019 ± 0.034
2.49IleVal: 2.49 ± 0.038
0.354IleTrp: 0.354 ± 0.014
0.496IleTyr: 0.496 ± 0.018
0.0IleXaa: 0.0 ± 0.0
Lys
3.051LysAla: 3.051 ± 0.055
0.137LysCys: 0.137 ± 0.009
1.507LysAsp: 1.507 ± 0.044
1.311LysGlu: 1.311 ± 0.032
0.505LysPhe: 0.505 ± 0.018
2.037LysGly: 2.037 ± 0.049
0.441LysHis: 0.441 ± 0.016
0.846LysIle: 0.846 ± 0.028
0.992LysLys: 0.992 ± 0.041
2.082LysLeu: 2.082 ± 0.037
0.405LysMet: 0.405 ± 0.013
0.558LysAsn: 0.558 ± 0.02
1.497LysPro: 1.497 ± 0.031
0.721LysGln: 0.721 ± 0.021
1.519LysArg: 1.519 ± 0.029
1.379LysSer: 1.379 ± 0.034
1.294LysThr: 1.294 ± 0.025
1.964LysVal: 1.964 ± 0.039
0.286LysTrp: 0.286 ± 0.012
0.496LysTyr: 0.496 ± 0.018
0.0LysXaa: 0.0 ± 0.0
Leu
15.108LeuAla: 15.108 ± 0.123
0.856LeuCys: 0.856 ± 0.02
6.864LeuAsp: 6.864 ± 0.062
4.644LeuGlu: 4.644 ± 0.055
2.599LeuPhe: 2.599 ± 0.041
9.508LeuGly: 9.508 ± 0.074
2.378LeuHis: 2.378 ± 0.042
3.107LeuIle: 3.107 ± 0.045
2.023LeuLys: 2.023 ± 0.037
11.254LeuLeu: 11.254 ± 0.112
1.555LeuMet: 1.555 ± 0.028
1.725LeuAsn: 1.725 ± 0.03
6.587LeuPro: 6.587 ± 0.064
2.115LeuGln: 2.115 ± 0.027
8.811LeuArg: 8.811 ± 0.091
5.419LeuSer: 5.419 ± 0.052
6.768LeuThr: 6.768 ± 0.062
8.577LeuVal: 8.577 ± 0.07
1.308LeuTrp: 1.308 ± 0.025
1.859LeuTyr: 1.859 ± 0.03
0.0LeuXaa: 0.0 ± 0.0
Met
2.167MetAla: 2.167 ± 0.034
0.146MetCys: 0.146 ± 0.008
0.895MetAsp: 0.895 ± 0.022
0.702MetGlu: 0.702 ± 0.018
0.446MetPhe: 0.446 ± 0.015
1.287MetGly: 1.287 ± 0.028
0.345MetHis: 0.345 ± 0.014
0.581MetIle: 0.581 ± 0.019
0.441MetLys: 0.441 ± 0.016
1.603MetLeu: 1.603 ± 0.028
0.271MetMet: 0.271 ± 0.011
0.433MetAsn: 0.433 ± 0.014
1.082MetPro: 1.082 ± 0.023
0.428MetGln: 0.428 ± 0.015
1.438MetArg: 1.438 ± 0.026
1.303MetSer: 1.303 ± 0.022
1.411MetThr: 1.411 ± 0.03
1.205MetVal: 1.205 ± 0.029
0.216MetTrp: 0.216 ± 0.01
0.305MetTyr: 0.305 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.125AsnAla: 2.125 ± 0.029
0.188AsnCys: 0.188 ± 0.011
0.981AsnAsp: 0.981 ± 0.022
0.92AsnGlu: 0.92 ± 0.024
0.479AsnPhe: 0.479 ± 0.017
1.956AsnGly: 1.956 ± 0.035
0.392AsnHis: 0.392 ± 0.015
0.627AsnIle: 0.627 ± 0.018
0.449AsnLys: 0.449 ± 0.016
1.671AsnLeu: 1.671 ± 0.032
0.3AsnMet: 0.3 ± 0.011
0.433AsnAsn: 0.433 ± 0.017
1.411AsnPro: 1.411 ± 0.025
0.514AsnGln: 0.514 ± 0.016
1.325AsnArg: 1.325 ± 0.024
1.048AsnSer: 1.048 ± 0.023
1.11AsnThr: 1.11 ± 0.026
1.316AsnVal: 1.316 ± 0.027
0.323AsnTrp: 0.323 ± 0.013
0.42AsnTyr: 0.42 ± 0.013
0.0AsnXaa: 0.0 ± 0.0
Pro
8.487ProAla: 8.487 ± 0.087
0.334ProCys: 0.334 ± 0.013
4.363ProAsp: 4.363 ± 0.045
4.853ProGlu: 4.853 ± 0.051
1.486ProPhe: 1.486 ± 0.031
7.072ProGly: 7.072 ± 0.099
1.465ProHis: 1.465 ± 0.031
1.11ProIle: 1.11 ± 0.024
1.297ProLys: 1.297 ± 0.032
5.441ProLeu: 5.441 ± 0.05
0.924ProMet: 0.924 ± 0.021
0.972ProAsn: 0.972 ± 0.022
3.508ProPro: 3.508 ± 0.069
1.973ProGln: 1.973 ± 0.042
4.007ProArg: 4.007 ± 0.056
3.273ProSer: 3.273 ± 0.043
3.225ProThr: 3.225 ± 0.044
5.363ProVal: 5.363 ± 0.052
0.884ProTrp: 0.884 ± 0.022
1.351ProTyr: 1.351 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
3.634GlnAla: 3.634 ± 0.047
0.185GlnCys: 0.185 ± 0.011
1.473GlnAsp: 1.473 ± 0.028
1.618GlnGlu: 1.618 ± 0.029
0.703GlnPhe: 0.703 ± 0.02
2.507GlnGly: 2.507 ± 0.043
0.694GlnHis: 0.694 ± 0.017
1.037GlnIle: 1.037 ± 0.024
0.624GlnLys: 0.624 ± 0.019
3.237GlnLeu: 3.237 ± 0.04
0.466GlnMet: 0.466 ± 0.017
0.509GlnAsn: 0.509 ± 0.014
1.77GlnPro: 1.77 ± 0.045
1.3GlnGln: 1.3 ± 0.035
2.472GlnArg: 2.472 ± 0.033
1.395GlnSer: 1.395 ± 0.027
1.291GlnThr: 1.291 ± 0.026
2.397GlnVal: 2.397 ± 0.039
0.503GlnTrp: 0.503 ± 0.016
0.607GlnTyr: 0.607 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
9.939ArgAla: 9.939 ± 0.102
0.592ArgCys: 0.592 ± 0.017
4.217ArgAsp: 4.217 ± 0.056
5.3ArgGlu: 5.3 ± 0.061
2.333ArgPhe: 2.333 ± 0.031
6.033ArgGly: 6.033 ± 0.06
2.104ArgHis: 2.104 ± 0.038
3.247ArgIle: 3.247 ± 0.041
1.739ArgLys: 1.739 ± 0.036
8.84ArgLeu: 8.84 ± 0.088
1.695ArgMet: 1.695 ± 0.029
1.365ArgAsn: 1.365 ± 0.031
4.919ArgPro: 4.919 ± 0.058
2.312ArgGln: 2.312 ± 0.038
7.689ArgArg: 7.689 ± 0.077
4.157ArgSer: 4.157 ± 0.046
5.544ArgThr: 5.544 ± 0.06
5.765ArgVal: 5.765 ± 0.056
1.317ArgTrp: 1.317 ± 0.027
1.839ArgTyr: 1.839 ± 0.03
0.0ArgXaa: 0.0 ± 0.0
Ser
7.164SerAla: 7.164 ± 0.065
0.445SerCys: 0.445 ± 0.016
2.745SerAsp: 2.745 ± 0.039
2.774SerGlu: 2.774 ± 0.037
1.532SerPhe: 1.532 ± 0.025
6.465SerGly: 6.465 ± 0.076
1.068SerHis: 1.068 ± 0.023
1.301SerIle: 1.301 ± 0.028
1.111SerLys: 1.111 ± 0.028
5.087SerLeu: 5.087 ± 0.052
1.072SerMet: 1.072 ± 0.023
0.862SerAsn: 0.862 ± 0.02
3.328SerPro: 3.328 ± 0.041
1.335SerGln: 1.335 ± 0.029
3.838SerArg: 3.838 ± 0.043
3.041SerSer: 3.041 ± 0.052
3.045SerThr: 3.045 ± 0.041
4.308SerVal: 4.308 ± 0.048
0.905SerTrp: 0.905 ± 0.02
1.224SerTyr: 1.224 ± 0.025
0.0SerXaa: 0.0 ± 0.0
Thr
8.442ThrAla: 8.442 ± 0.078
0.439ThrCys: 0.439 ± 0.017
3.65ThrAsp: 3.65 ± 0.042
3.705ThrGlu: 3.705 ± 0.051
1.564ThrPhe: 1.564 ± 0.03
6.757ThrGly: 6.757 ± 0.06
1.209ThrHis: 1.209 ± 0.026
1.477ThrIle: 1.477 ± 0.03
1.256ThrLys: 1.256 ± 0.031
5.684ThrLeu: 5.684 ± 0.053
0.902ThrMet: 0.902 ± 0.019
0.999ThrAsn: 0.999 ± 0.025
3.967ThrPro: 3.967 ± 0.047
1.335ThrGln: 1.335 ± 0.028
3.873ThrArg: 3.873 ± 0.046
3.182ThrSer: 3.182 ± 0.04
3.564ThrThr: 3.564 ± 0.045
5.821ThrVal: 5.821 ± 0.053
0.849ThrTrp: 0.849 ± 0.02
1.339ThrTyr: 1.339 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
10.084ValAla: 10.084 ± 0.083
0.75ValCys: 0.75 ± 0.018
4.867ValAsp: 4.867 ± 0.047
4.654ValGlu: 4.654 ± 0.05
2.34ValPhe: 2.34 ± 0.037
6.138ValGly: 6.138 ± 0.06
2.041ValHis: 2.041 ± 0.029
2.649ValIle: 2.649 ± 0.038
1.738ValLys: 1.738 ± 0.036
9.223ValLeu: 9.223 ± 0.081
1.343ValMet: 1.343 ± 0.027
1.605ValAsn: 1.605 ± 0.032
5.197ValPro: 5.197 ± 0.053
2.113ValGln: 2.113 ± 0.028
7.187ValArg: 7.187 ± 0.069
4.243ValSer: 4.243 ± 0.049
5.277ValThr: 5.277 ± 0.052
7.423ValVal: 7.423 ± 0.074
1.072ValTrp: 1.072 ± 0.021
1.473ValTyr: 1.473 ± 0.031
0.0ValXaa: 0.0 ± 0.0
Trp
1.534TrpAla: 1.534 ± 0.028
0.157TrpCys: 0.157 ± 0.008
0.826TrpAsp: 0.826 ± 0.023
0.773TrpGlu: 0.773 ± 0.022
0.518TrpPhe: 0.518 ± 0.016
1.071TrpGly: 1.071 ± 0.023
0.382TrpHis: 0.382 ± 0.013
0.537TrpIle: 0.537 ± 0.018
0.361TrpLys: 0.361 ± 0.013
1.72TrpLeu: 1.72 ± 0.031
0.289TrpMet: 0.289 ± 0.01
0.408TrpAsn: 0.408 ± 0.015
0.772TrpPro: 0.772 ± 0.02
0.686TrpGln: 0.686 ± 0.02
1.297TrpArg: 1.297 ± 0.024
0.958TrpSer: 0.958 ± 0.019
0.97TrpThr: 0.97 ± 0.023
0.923TrpVal: 0.923 ± 0.021
0.319TrpTrp: 0.319 ± 0.013
0.356TrpTyr: 0.356 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.731TyrAla: 2.731 ± 0.039
0.177TyrCys: 0.177 ± 0.009
1.425TyrAsp: 1.425 ± 0.029
1.431TyrGlu: 1.431 ± 0.03
0.636TyrPhe: 0.636 ± 0.018
2.246TyrGly: 2.246 ± 0.035
0.38TyrHis: 0.38 ± 0.012
0.452TyrIle: 0.452 ± 0.016
0.393TyrLys: 0.393 ± 0.017
2.024TyrLeu: 2.024 ± 0.031
0.251TyrMet: 0.251 ± 0.012
0.389TyrAsn: 0.389 ± 0.015
1.055TyrPro: 1.055 ± 0.022
0.576TyrGln: 0.576 ± 0.016
1.866TyrArg: 1.866 ± 0.034
0.944TyrSer: 0.944 ± 0.022
1.128TyrThr: 1.128 ± 0.023
1.619TyrVal: 1.619 ± 0.027
0.369TyrTrp: 0.369 ± 0.015
0.443TyrTyr: 0.443 ± 0.017
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6361 proteins (2163193 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski