Achromobacter piechaudii ATCC 43553
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5755 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D4XJT6|D4XJT6_9BURK VCBS repeat (3 repeats) (Fragment) OS=Achromobacter piechaudii ATCC 43553 OX=742159 GN=HMPREF0004_5733 PE=4 SV=1
MM1 pKa = 6.98 FAAAEE6 pKa = 4.04 PDD8 pKa = 3.17 IDD10 pKa = 3.89 EE11 pKa = 4.82 AGCFFRR17 pKa = 11.84 CPGCDD22 pKa = 3.48 HH23 pKa = 6.98 RR24 pKa = 11.84 NKK26 pKa = 10.37 LINVGPYY33 pKa = 10.32 GDD35 pKa = 4.88 EE36 pKa = 4.28 DD37 pKa = 5.86 PITLGQPDD45 pKa = 3.84 DD46 pKa = 3.71
Molecular weight: 5.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.766
IPC2_protein 3.961
IPC_protein 3.859
Toseland 3.643
ProMoST 3.935
Dawson 3.872
Bjellqvist 4.113
Wikipedia 3.846
Rodwell 3.694
Grimsley 3.567
Solomon 3.834
Lehninger 3.795
Nozaki 3.999
DTASelect 4.24
Thurlkill 3.732
EMBOSS 3.846
Sillero 3.986
Patrickios 1.926
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.926
Protein with the highest isoelectric point:
>tr|D4X3F8|D4X3F8_9BURK ATPase family associated with various cellular activities (AAA) (Fragment) OS=Achromobacter piechaudii ATCC 43553 OX=742159 GN=HMPREF0004_0005 PE=3 SV=1
NN1 pKa = 7.35 APSRR5 pKa = 11.84 PASLQRR11 pKa = 11.84 SSTMSRR17 pKa = 11.84 TAAAARR23 pKa = 11.84 GSPTTSPRR31 pKa = 11.84 RR32 pKa = 11.84 TRR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 9.03 MGPP39 pKa = 3.07
Molecular weight: 4.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.489
IPC2_protein 11.082
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.32
Rodwell 12.398
Grimsley 12.881
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.149
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.149
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5755
0
5755
1831974
39
9024
318.3
34.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.896 ± 0.046
0.88 ± 0.01
5.482 ± 0.031
4.814 ± 0.036
3.391 ± 0.026
8.47 ± 0.049
2.119 ± 0.016
4.487 ± 0.023
2.988 ± 0.026
10.639 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.568 ± 0.02
2.718 ± 0.028
5.317 ± 0.033
3.959 ± 0.02
6.884 ± 0.041
5.525 ± 0.039
5.318 ± 0.052
7.706 ± 0.031
1.418 ± 0.017
2.423 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here