Flavobacterium crassostreae

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2424 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B9E7Y6|A0A1B9E7Y6_9FLAO Glyceraldehyde-3-phosphate dehydrogenase OS=Flavobacterium crassostreae OX=1763534 GN=LPBF_03630 PE=3 SV=1
MM1 pKa = 7.29ATLLFTPRR9 pKa = 11.84TTIDD13 pKa = 3.15ANIFQYY19 pKa = 11.08RR20 pKa = 11.84LDD22 pKa = 3.77NSPFHH27 pKa = 6.91AEE29 pKa = 3.73WNIEE33 pKa = 3.93TGSYY37 pKa = 9.8EE38 pKa = 4.17FEE40 pKa = 4.35EE41 pKa = 4.83EE42 pKa = 4.11EE43 pKa = 4.32QSIDD47 pKa = 3.67QLEE50 pKa = 4.26EE51 pKa = 3.73EE52 pKa = 4.69LALSITYY59 pKa = 9.76DD60 pKa = 2.94INGYY64 pKa = 10.17FEE66 pKa = 4.38LQNN69 pKa = 3.57

Molecular weight: 8.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B9E7G3|A0A1B9E7G3_9FLAO GTP cyclohydrolase 1 OS=Flavobacterium crassostreae OX=1763534 GN=folE PE=3 SV=1
MM1 pKa = 7.47AADD4 pKa = 3.7SPQRR8 pKa = 11.84PFLGARR14 pKa = 11.84TWSVQPDD21 pKa = 3.62PAGVYY26 pKa = 9.82VGGMRR31 pKa = 11.84PNFGVDD37 pKa = 2.76INQKK41 pKa = 7.34RR42 pKa = 11.84TARR45 pKa = 11.84IAVRR49 pKa = 11.84RR50 pKa = 11.84PFFGCCVLGMHH61 pKa = 7.43

Molecular weight: 6.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2424

0

2424

798124

46

4689

329.3

37.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.095 ± 0.049

0.789 ± 0.017

5.034 ± 0.034

6.091 ± 0.056

5.177 ± 0.043

5.969 ± 0.053

1.795 ± 0.024

8.068 ± 0.046

8.094 ± 0.062

9.663 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.202 ± 0.029

5.955 ± 0.045

3.436 ± 0.032

4.031 ± 0.035

3.157 ± 0.03

6.166 ± 0.038

6.107 ± 0.069

6.195 ± 0.041

0.955 ± 0.015

4.023 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski