Catalinimonas alkaloidigena
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5672 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G9RI04|A0A1G9RI04_9BACT PAS domain S-box-containing protein OS=Catalinimonas alkaloidigena OX=1075417 GN=SAMN05421823_111167 PE=4 SV=1
MM1 pKa = 7.1 LTQVRR6 pKa = 11.84 SRR8 pKa = 11.84 LGAFLVLAGLSSVSLAQTPVFINEE32 pKa = 3.39 IHH34 pKa = 6.5 YY35 pKa = 11.13 DD36 pKa = 3.61 NVGTDD41 pKa = 2.96 TGEE44 pKa = 4.47 AIEE47 pKa = 4.25 IAGPAGTDD55 pKa = 3.26 LTGWSLVLYY64 pKa = 10.05 NGSNNQPYY72 pKa = 7.17 TTSALSGTLADD83 pKa = 4.38 AGSGYY88 pKa = 10.68 GFAVVTYY95 pKa = 8.29 PSNGIQNGDD104 pKa = 3.67 PDD106 pKa = 4.27 GVALVNSAGAVIQFLSYY123 pKa = 10.95 DD124 pKa = 3.58 GTMTAATGPAQGLTSIDD141 pKa = 3.2 IGVGEE146 pKa = 4.75 SNATTTGFSLQLTGSGSSYY165 pKa = 11.55 EE166 pKa = 4.12 DD167 pKa = 3.52 FTWADD172 pKa = 3.17 AQANTFGAVNAGQTFGGGPVGPADD196 pKa = 3.42 PVINEE201 pKa = 4.36 FSASTTGTDD210 pKa = 3.05 VEE212 pKa = 4.58 YY213 pKa = 10.7 IEE215 pKa = 5.84 ILGDD219 pKa = 3.63 PNADD223 pKa = 3.56 YY224 pKa = 11.65 SDD226 pKa = 3.77 LTVLEE231 pKa = 4.59 IEE233 pKa = 5.01 GDD235 pKa = 3.73 TPAQGTIDD243 pKa = 4.31 GIFPLGTTDD252 pKa = 3.23 ANGYY256 pKa = 9.63 FLLNLPANEE265 pKa = 4.74 LEE267 pKa = 4.38 NGTLTLLLVRR277 pKa = 11.84 NFADD281 pKa = 3.54 TLGADD286 pKa = 4.42 LDD288 pKa = 4.17 ADD290 pKa = 3.8 NDD292 pKa = 4.07 GVLDD296 pKa = 3.71 VTPWTDD302 pKa = 3.43 LVDD305 pKa = 5.45 GIAVNDD311 pKa = 4.45 GGAGDD316 pKa = 3.47 QTYY319 pKa = 10.86 AVTLNVGYY327 pKa = 10.37 DD328 pKa = 3.74 GLSFAPGGASRR339 pKa = 11.84 LPNGTDD345 pKa = 3.3 TDD347 pKa = 3.92 QTSDD351 pKa = 2.61 WTRR354 pKa = 11.84 NDD356 pKa = 2.99 FDD358 pKa = 4.27 LAGIDD363 pKa = 4.08 GYY365 pKa = 10.93 EE366 pKa = 4.21 GSPVPGEE373 pKa = 4.4 AYY375 pKa = 7.71 NTPGAPNQAVAAPLPAIVINEE396 pKa = 4.27 LDD398 pKa = 3.54 VDD400 pKa = 4.23 QDD402 pKa = 3.58 GTDD405 pKa = 2.96 AAEE408 pKa = 4.79 FIEE411 pKa = 5.69 LYY413 pKa = 10.76 DD414 pKa = 3.88 GGVGNTALNGLVLVLFNGNGDD435 pKa = 3.63 EE436 pKa = 4.48 AYY438 pKa = 10.45 GAIALDD444 pKa = 4.92 GYY446 pKa = 7.62 TTDD449 pKa = 3.24 AEE451 pKa = 5.33 GYY453 pKa = 9.72 FVVGSANVANVDD465 pKa = 3.79 LVAFTTNGLQNGADD479 pKa = 3.56 AVALYY484 pKa = 10.44 QGDD487 pKa = 3.59 AAAITTVTTDD497 pKa = 3.26 GLIDD501 pKa = 3.54 AVVYY505 pKa = 7.59 GTSDD509 pKa = 3.47 ADD511 pKa = 4.61 DD512 pKa = 4.36 EE513 pKa = 4.77 GLLALLNAGQPQADD527 pKa = 3.85 EE528 pKa = 4.27 NANGQAISEE537 pKa = 4.31 SLQRR541 pKa = 11.84 LPNGSGGQRR550 pKa = 11.84 NTDD553 pKa = 3.34 TFGAAAPTPGTEE565 pKa = 3.95 NGGDD569 pKa = 3.7 GGPVEE574 pKa = 4.56 PQPGLITIAEE584 pKa = 4.19 ARR586 pKa = 11.84 NAADD590 pKa = 3.94 DD591 pKa = 4.09 AVVTIQGILTVADD604 pKa = 3.96 EE605 pKa = 4.9 FGGPAYY611 pKa = 9.82 IQDD614 pKa = 3.97 STGGIAVFDD623 pKa = 4.16 AALHH627 pKa = 5.97 GGTYY631 pKa = 10.24 QIGDD635 pKa = 3.81 EE636 pKa = 4.4 VKK638 pKa = 10.02 LTGALDD644 pKa = 3.73 TYY646 pKa = 10.97 NGLRR650 pKa = 11.84 EE651 pKa = 4.46 VVSVTHH657 pKa = 7.15 DD658 pKa = 3.67 SLVSTGNSVTPAAITVAQLAAYY680 pKa = 7.92 EE681 pKa = 4.37 GQLVTLQNVSFPNPGDD697 pKa = 3.96 LFWPNSNFVITDD709 pKa = 3.31 ATGSGEE715 pKa = 4.08 LRR717 pKa = 11.84 IDD719 pKa = 3.76 ADD721 pKa = 3.81 VEE723 pKa = 4.57 GIAGKK728 pKa = 9.59 AQPEE732 pKa = 4.47 TCEE735 pKa = 3.84 VTGVIGQFNGTPQLLPRR752 pKa = 11.84 LAGDD756 pKa = 4.36 LPCAEE761 pKa = 5.17 DD762 pKa = 4.19 FVPPTGDD769 pKa = 2.96 TDD771 pKa = 4.29 LPLAQTLDD779 pKa = 3.58 VVTWNVEE786 pKa = 3.95 WFGSPSQGPTNEE798 pKa = 3.91 TLQADD803 pKa = 3.87 NVKK806 pKa = 10.35 RR807 pKa = 11.84 VLDD810 pKa = 3.8 SLDD813 pKa = 3.49 ADD815 pKa = 3.98 VYY817 pKa = 11.04 ALEE820 pKa = 5.14 EE821 pKa = 4.28 ISDD824 pKa = 4.05 SALLQQVVDD833 pKa = 3.98 QLPGYY838 pKa = 10.36 ALAIQTGYY846 pKa = 10.84 VSYY849 pKa = 10.25 PPNEE853 pKa = 3.74 PRR855 pKa = 11.84 EE856 pKa = 4.19 SQKK859 pKa = 11.01 LAFIYY864 pKa = 9.12 KK865 pKa = 8.65 TEE867 pKa = 4.2 TVSPVRR873 pKa = 11.84 TQPLLDD879 pKa = 3.63 WQHH882 pKa = 6.54 PYY884 pKa = 11.41 YY885 pKa = 10.97 NGGDD889 pKa = 3.58 DD890 pKa = 5.6 SYY892 pKa = 11.32 LTDD895 pKa = 3.73 YY896 pKa = 10.52 PADD899 pKa = 3.53 DD900 pKa = 3.54 RR901 pKa = 11.84 TRR903 pKa = 11.84 FFASGRR909 pKa = 11.84 LPFLMVADD917 pKa = 3.87 VTIDD921 pKa = 3.19 GSTEE925 pKa = 3.64 RR926 pKa = 11.84 IYY928 pKa = 10.84 FVGLHH933 pKa = 5.61 ARR935 pKa = 11.84 ANGSDD940 pKa = 3.36 AQLTYY945 pKa = 11.61 DD946 pKa = 3.27 MRR948 pKa = 11.84 KK949 pKa = 9.82 YY950 pKa = 10.64 DD951 pKa = 3.81 VEE953 pKa = 4.01 RR954 pKa = 11.84 LKK956 pKa = 11.19 AVLDD960 pKa = 3.82 TLGGGTANVMLLGDD974 pKa = 3.99 YY975 pKa = 11.01 NDD977 pKa = 5.26 DD978 pKa = 3.4 VDD980 pKa = 3.95 EE981 pKa = 4.46 TVANISSTVTSYY993 pKa = 11.4 AAYY996 pKa = 10.41 VQDD999 pKa = 5.28 DD1000 pKa = 4.33 SLNPGADD1007 pKa = 3.14 GNYY1010 pKa = 9.36 YY1011 pKa = 10.84 AVLTSSLSDD1020 pKa = 2.94 AGFRR1024 pKa = 11.84 TYY1026 pKa = 11.05 VSYY1029 pKa = 11.42 EE1030 pKa = 3.73 NVIDD1034 pKa = 5.1 HH1035 pKa = 6.19 ITVTDD1040 pKa = 4.01 EE1041 pKa = 4.02 LTDD1044 pKa = 3.56 NYY1046 pKa = 9.67 IANSATVHH1054 pKa = 5.61 YY1055 pKa = 9.88 EE1056 pKa = 3.75 VFAGNSVIPNYY1067 pKa = 10.99 ASTTSDD1073 pKa = 3.04 HH1074 pKa = 6.34 MPVSVRR1080 pKa = 11.84 LVLEE1084 pKa = 4.04 KK1085 pKa = 10.95 APALTLGSCVYY1096 pKa = 9.72 TVEE1099 pKa = 4.78 DD1100 pKa = 4.35 FPGAATITALDD1111 pKa = 3.9 SVTYY1115 pKa = 9.8 WDD1117 pKa = 4.22 FFQRR1121 pKa = 11.84 RR1122 pKa = 11.84 GSYY1125 pKa = 8.88 TVGVATGVRR1134 pKa = 11.84 TDD1136 pKa = 3.5 GQAGAWEE1143 pKa = 4.03 IHH1145 pKa = 6.38 NDD1147 pKa = 3.61 CSIKK1151 pKa = 10.19 PLRR1154 pKa = 11.84 KK1155 pKa = 9.71 SGSKK1159 pKa = 10.49 QNTSLLPDD1167 pKa = 3.23 VLGAKK1172 pKa = 10.12 RR1173 pKa = 11.84 KK1174 pKa = 10.21 DD1175 pKa = 3.01 GWHH1178 pKa = 5.87 YY1179 pKa = 10.65 EE1180 pKa = 4.08 PYY1182 pKa = 10.41 AISDD1186 pKa = 3.56 DD1187 pKa = 4.06 GEE1189 pKa = 4.47 YY1190 pKa = 10.59 ILANAINTDD1199 pKa = 3.33 GYY1201 pKa = 9.81 TIGRR1205 pKa = 11.84 WRR1207 pKa = 11.84 GVAPGATLPVKK1218 pKa = 10.38 FRR1220 pKa = 11.84 IGGPLLGRR1228 pKa = 11.84 VFILLGTVDD1237 pKa = 4.74 CDD1239 pKa = 3.75 DD1240 pKa = 5.81 LAMEE1244 pKa = 4.39 WTDD1247 pKa = 3.88 GRR1249 pKa = 11.84 YY1250 pKa = 9.95 LLTGCDD1256 pKa = 3.48 ASEE1259 pKa = 4.03 EE1260 pKa = 4.42 VPTRR1264 pKa = 11.84 VIASVPVQTAQTHH1277 pKa = 6.83 LYY1279 pKa = 8.73 PNPARR1284 pKa = 11.84 QSITVQFAEE1293 pKa = 4.48 SLQSNATLRR1302 pKa = 11.84 VVDD1305 pKa = 3.89 ALGRR1309 pKa = 11.84 LYY1311 pKa = 10.98 AEE1313 pKa = 4.19 RR1314 pKa = 11.84 TVEE1317 pKa = 3.95 AGQVEE1322 pKa = 4.57 YY1323 pKa = 10.99 QEE1325 pKa = 4.35 QLSNLPSGIYY1335 pKa = 10.29 FMQVQTAEE1343 pKa = 4.42 GVQNLRR1349 pKa = 11.84 FVKK1352 pKa = 10.31 QQ1353 pKa = 3.15
Molecular weight: 142.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.745
IPC_protein 3.795
Toseland 3.554
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.719
Rodwell 3.605
Grimsley 3.465
Solomon 3.783
Lehninger 3.745
Nozaki 3.884
DTASelect 4.164
Thurlkill 3.605
EMBOSS 3.732
Sillero 3.91
Patrickios 0.858
IPC_peptide 3.783
IPC2_peptide 3.884
IPC2.peptide.svr19 3.802
Protein with the highest isoelectric point:
>tr|A0A1G8ZXG0|A0A1G8ZXG0_9BACT DNA-binding response regulator OmpR family contains REC and winged-helix (WHTH) domain OS=Catalinimonas alkaloidigena OX=1075417 GN=SAMN05421823_102117 PE=4 SV=1
MM1 pKa = 7.5 RR2 pKa = 11.84 QPVDD6 pKa = 2.91 HH7 pKa = 6.34 STIANVLSSRR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 8.91 ATTHH23 pKa = 5.88 AQNPPPKK30 pKa = 9.51 EE31 pKa = 3.83 RR32 pKa = 11.84 RR33 pKa = 11.84 LHH35 pKa = 5.8 YY36 pKa = 10.3 SLHH39 pKa = 5.63 TAVTPTLRR47 pKa = 11.84 TLEE50 pKa = 4.48 HH51 pKa = 6.87 DD52 pKa = 5.04 HH53 pKa = 6.63 ICLAYY58 pKa = 9.84 RR59 pKa = 11.84 VWGTGTHH66 pKa = 6.58 PMLAFHH72 pKa = 7.21 GFGQTGAHH80 pKa = 6.54 FAALQPAWEE89 pKa = 4.29 HH90 pKa = 5.17 TFRR93 pKa = 11.84 VYY95 pKa = 11.15 AFDD98 pKa = 4.79 LFCHH102 pKa = 6.68 GDD104 pKa = 3.7 SAWPHH109 pKa = 4.5 GTMPLNFATWTTLMQRR125 pKa = 11.84 FCQQEE130 pKa = 4.25 KK131 pKa = 10.23 IDD133 pKa = 4.1 MFSAAGFSLGSRR145 pKa = 11.84 LALATATAFPQQLTSLTLVAPDD167 pKa = 4.61 SPFLSPWYY175 pKa = 8.99 RR176 pKa = 11.84 LAMAWPRR183 pKa = 11.84 LFRR186 pKa = 11.84 WSLSHH191 pKa = 7.29 SDD193 pKa = 3.65 VWLSWITRR201 pKa = 11.84 LEE203 pKa = 4.11 QTGVLAPAAARR214 pKa = 11.84 LALRR218 pKa = 11.84 SLRR221 pKa = 11.84 QPAHH225 pKa = 7.07 RR226 pKa = 11.84 ARR228 pKa = 11.84 MACAWPVFSALRR240 pKa = 11.84 FSPVSLSQLFEE251 pKa = 3.86 RR252 pKa = 11.84 HH253 pKa = 5.49 AVSVQLFLGKK263 pKa = 9.86 NDD265 pKa = 4.86 PIAAEE270 pKa = 4.11 RR271 pKa = 11.84 KK272 pKa = 8.8 LIAWVGPMTRR282 pKa = 11.84 GKK284 pKa = 8.92 VTLLDD289 pKa = 3.87 VPHH292 pKa = 6.75 EE293 pKa = 4.1 RR294 pKa = 11.84 LLAVVAGHH302 pKa = 7.09 WPQNN306 pKa = 3.52
Molecular weight: 34.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.531
IPC_protein 10.496
Toseland 10.599
ProMoST 10.379
Dawson 10.701
Bjellqvist 10.467
Wikipedia 10.95
Rodwell 10.774
Grimsley 10.76
Solomon 10.847
Lehninger 10.804
Nozaki 10.613
DTASelect 10.452
Thurlkill 10.599
EMBOSS 11.008
Sillero 10.643
Patrickios 10.482
IPC_peptide 10.847
IPC2_peptide 9.736
IPC2.peptide.svr19 8.605
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5672
0
5672
2097683
25
6554
369.8
41.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.819 ± 0.033
0.737 ± 0.012
5.43 ± 0.026
6.082 ± 0.032
4.433 ± 0.022
7.229 ± 0.04
2.328 ± 0.018
4.879 ± 0.025
4.016 ± 0.035
10.56 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.095 ± 0.015
3.889 ± 0.034
4.782 ± 0.023
4.736 ± 0.027
5.896 ± 0.028
5.589 ± 0.022
6.097 ± 0.039
7.003 ± 0.028
1.496 ± 0.014
3.903 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here