Salmonella phage SE16
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A513ZX01|A0A513ZX01_9CAUD Uncharacterized protein OS=Salmonella phage SE16 OX=2575326 PE=4 SV=1
MM1 pKa = 7.52 IAMLSPTQIMQYY13 pKa = 9.75 QKK15 pKa = 10.94 EE16 pKa = 4.53 SVDD19 pKa = 4.19 RR20 pKa = 11.84 ALTCANCGQKK30 pKa = 10.32 LHH32 pKa = 5.86 VLEE35 pKa = 4.29 VHH37 pKa = 5.94 VCEE40 pKa = 4.68 ACCSEE45 pKa = 5.43 LMSDD49 pKa = 4.48 PNSAMYY55 pKa = 10.4 EE56 pKa = 4.1 EE57 pKa = 5.48 EE58 pKa = 5.11 DD59 pKa = 5.44 DD60 pKa = 4.54 EE61 pKa = 7.52 DD62 pKa = 5.12 DD63 pKa = 3.93 FAPGKK68 pKa = 10.34 EE69 pKa = 4.16 DD70 pKa = 3.16
Molecular weight: 7.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.86
IPC2_protein 4.037
IPC_protein 3.935
Toseland 3.757
ProMoST 3.999
Dawson 3.91
Bjellqvist 4.126
Wikipedia 3.821
Rodwell 3.783
Grimsley 3.681
Solomon 3.897
Lehninger 3.846
Nozaki 4.037
DTASelect 4.19
Thurlkill 3.808
EMBOSS 3.834
Sillero 4.062
Patrickios 0.54
IPC_peptide 3.897
IPC2_peptide 4.037
IPC2.peptide.svr19 3.993
Protein with the highest isoelectric point:
>tr|A0A513ZX13|A0A513ZX13_9CAUD Repressor OS=Salmonella phage SE16 OX=2575326 PE=4 SV=1
MM1 pKa = 7.54 RR2 pKa = 11.84 RR3 pKa = 11.84 QRR5 pKa = 11.84 RR6 pKa = 11.84 SFTDD10 pKa = 4.5 IICEE14 pKa = 3.91 NCKK17 pKa = 10.21 YY18 pKa = 10.77 LPTKK22 pKa = 10.48 RR23 pKa = 11.84 SRR25 pKa = 11.84 NKK27 pKa = 9.55 PKK29 pKa = 10.29 PIPKK33 pKa = 9.96 EE34 pKa = 3.93 SDD36 pKa = 3.19 VKK38 pKa = 9.6 TFNYY42 pKa = 7.51 TAHH45 pKa = 6.73 LWDD48 pKa = 3.94 IRR50 pKa = 11.84 WLRR53 pKa = 11.84 HH54 pKa = 5.19 RR55 pKa = 11.84 ARR57 pKa = 11.84 KK58 pKa = 8.96
Molecular weight: 7.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.3
IPC2_protein 9.897
IPC_protein 10.906
Toseland 11.14
ProMoST 11.169
Dawson 11.184
Bjellqvist 10.979
Wikipedia 11.477
Rodwell 11.359
Grimsley 11.228
Solomon 11.447
Lehninger 11.389
Nozaki 11.14
DTASelect 10.979
Thurlkill 11.14
EMBOSS 11.579
Sillero 11.14
Patrickios 11.096
IPC_peptide 11.447
IPC2_peptide 10.218
IPC2.peptide.svr19 8.688
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
70
0
70
12639
38
725
180.6
20.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.043 ± 0.491
1.131 ± 0.155
5.95 ± 0.253
6.686 ± 0.384
3.394 ± 0.228
6.955 ± 0.421
1.685 ± 0.175
6.243 ± 0.348
6.67 ± 0.301
8.007 ± 0.252
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.062 ± 0.175
4.85 ± 0.28
3.909 ± 0.204
4.344 ± 0.327
5.974 ± 0.301
6.266 ± 0.266
4.961 ± 0.262
5.815 ± 0.303
1.677 ± 0.18
3.378 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here