Amino acid dipepetide frequency for Salmonella phage SE16

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.783AlaAla: 8.783 ± 1.345
1.029AlaCys: 1.029 ± 0.337
5.381AlaAsp: 5.381 ± 0.736
7.121AlaGlu: 7.121 ± 0.789
3.482AlaPhe: 3.482 ± 0.61
6.963AlaGly: 6.963 ± 0.877
0.95AlaHis: 0.95 ± 0.254
5.776AlaIle: 5.776 ± 0.675
4.589AlaLys: 4.589 ± 0.634
6.726AlaLeu: 6.726 ± 0.908
3.956AlaMet: 3.956 ± 0.677
5.776AlaAsn: 5.776 ± 0.882
2.611AlaPro: 2.611 ± 0.382
3.402AlaGln: 3.402 ± 0.53
5.064AlaArg: 5.064 ± 0.619
4.668AlaSer: 4.668 ± 0.616
4.985AlaThr: 4.985 ± 0.718
4.906AlaVal: 4.906 ± 0.597
1.424AlaTrp: 1.424 ± 0.341
2.849AlaTyr: 2.849 ± 0.443
0.0AlaXaa: 0.0 ± 0.0
Cys
0.712CysAla: 0.712 ± 0.261
0.237CysCys: 0.237 ± 0.142
0.633CysAsp: 0.633 ± 0.242
0.633CysGlu: 0.633 ± 0.207
0.396CysPhe: 0.396 ± 0.199
0.87CysGly: 0.87 ± 0.253
0.237CysHis: 0.237 ± 0.114
0.554CysIle: 0.554 ± 0.206
0.87CysLys: 0.87 ± 0.262
0.317CysLeu: 0.317 ± 0.159
0.396CysMet: 0.396 ± 0.173
0.554CysAsn: 0.554 ± 0.226
0.475CysPro: 0.475 ± 0.185
0.396CysGln: 0.396 ± 0.195
1.187CysArg: 1.187 ± 0.297
1.108CysSer: 1.108 ± 0.287
0.396CysThr: 0.396 ± 0.162
0.791CysVal: 0.791 ± 0.205
0.158CysTrp: 0.158 ± 0.121
0.396CysTyr: 0.396 ± 0.193
0.0CysXaa: 0.0 ± 0.0
Asp
7.517AspAla: 7.517 ± 0.92
0.475AspCys: 0.475 ± 0.179
4.035AspAsp: 4.035 ± 0.533
4.194AspGlu: 4.194 ± 0.665
1.899AspPhe: 1.899 ± 0.402
5.301AspGly: 5.301 ± 0.7
0.95AspHis: 0.95 ± 0.309
4.194AspIle: 4.194 ± 0.555
3.64AspLys: 3.64 ± 0.552
4.51AspLeu: 4.51 ± 0.582
1.662AspMet: 1.662 ± 0.379
2.216AspAsn: 2.216 ± 0.362
1.503AspPro: 1.503 ± 0.331
1.266AspGln: 1.266 ± 0.341
2.374AspArg: 2.374 ± 0.385
3.64AspSer: 3.64 ± 0.442
2.136AspThr: 2.136 ± 0.329
4.115AspVal: 4.115 ± 0.527
1.266AspTrp: 1.266 ± 0.326
2.611AspTyr: 2.611 ± 0.464
0.0AspXaa: 0.0 ± 0.0
Glu
6.172GluAla: 6.172 ± 0.652
1.424GluCys: 1.424 ± 0.305
3.402GluAsp: 3.402 ± 0.568
4.352GluGlu: 4.352 ± 0.733
1.978GluPhe: 1.978 ± 0.483
3.086GluGly: 3.086 ± 0.41
1.345GluHis: 1.345 ± 0.359
3.877GluIle: 3.877 ± 0.568
4.035GluLys: 4.035 ± 0.581
6.33GluLeu: 6.33 ± 0.662
2.374GluMet: 2.374 ± 0.452
3.64GluAsn: 3.64 ± 0.527
2.849GluPro: 2.849 ± 0.454
4.035GluGln: 4.035 ± 0.529
4.51GluArg: 4.51 ± 0.717
3.64GluSer: 3.64 ± 0.469
2.928GluThr: 2.928 ± 0.434
3.165GluVal: 3.165 ± 0.532
1.662GluTrp: 1.662 ± 0.379
2.057GluTyr: 2.057 ± 0.378
0.0GluXaa: 0.0 ± 0.0
Phe
3.323PheAla: 3.323 ± 0.489
0.87PheCys: 0.87 ± 0.274
2.453PheAsp: 2.453 ± 0.435
1.82PheGlu: 1.82 ± 0.358
1.029PhePhe: 1.029 ± 0.392
2.057PheGly: 2.057 ± 0.361
0.396PheHis: 0.396 ± 0.167
2.928PheIle: 2.928 ± 0.502
1.899PheLys: 1.899 ± 0.39
1.978PheLeu: 1.978 ± 0.405
1.187PheMet: 1.187 ± 0.282
2.216PheAsn: 2.216 ± 0.411
1.187PhePro: 1.187 ± 0.272
0.95PheGln: 0.95 ± 0.288
1.345PheArg: 1.345 ± 0.36
3.007PheSer: 3.007 ± 0.441
2.216PheThr: 2.216 ± 0.391
1.345PheVal: 1.345 ± 0.308
0.633PheTrp: 0.633 ± 0.169
1.108PheTyr: 1.108 ± 0.336
0.0PheXaa: 0.0 ± 0.0
Gly
5.222GlyAla: 5.222 ± 0.944
0.554GlyCys: 0.554 ± 0.199
3.086GlyAsp: 3.086 ± 0.582
4.589GlyGlu: 4.589 ± 0.549
2.69GlyPhe: 2.69 ± 0.463
5.934GlyGly: 5.934 ± 0.912
1.187GlyHis: 1.187 ± 0.297
4.906GlyIle: 4.906 ± 0.627
5.46GlyLys: 5.46 ± 0.583
4.431GlyLeu: 4.431 ± 0.878
2.532GlyMet: 2.532 ± 0.48
3.323GlyAsn: 3.323 ± 0.42
1.029GlyPro: 1.029 ± 0.229
3.323GlyGln: 3.323 ± 0.773
4.431GlyArg: 4.431 ± 0.628
4.668GlySer: 4.668 ± 0.785
3.244GlyThr: 3.244 ± 0.612
5.143GlyVal: 5.143 ± 0.608
1.583GlyTrp: 1.583 ± 0.318
2.216GlyTyr: 2.216 ± 0.393
0.0GlyXaa: 0.0 ± 0.0
His
0.87HisAla: 0.87 ± 0.261
0.317HisCys: 0.317 ± 0.125
1.266HisAsp: 1.266 ± 0.311
1.503HisGlu: 1.503 ± 0.344
0.633HisPhe: 0.633 ± 0.189
0.95HisGly: 0.95 ± 0.23
0.554HisHis: 0.554 ± 0.208
0.712HisIle: 0.712 ± 0.191
1.029HisLys: 1.029 ± 0.311
1.662HisLeu: 1.662 ± 0.403
0.712HisMet: 0.712 ± 0.237
0.554HisAsn: 0.554 ± 0.206
1.108HisPro: 1.108 ± 0.304
0.633HisGln: 0.633 ± 0.22
1.266HisArg: 1.266 ± 0.268
0.554HisSer: 0.554 ± 0.201
0.633HisThr: 0.633 ± 0.216
1.029HisVal: 1.029 ± 0.291
0.158HisTrp: 0.158 ± 0.104
0.712HisTyr: 0.712 ± 0.206
0.0HisXaa: 0.0 ± 0.0
Ile
6.172IleAla: 6.172 ± 0.779
0.475IleCys: 0.475 ± 0.172
4.194IleAsp: 4.194 ± 0.458
3.877IleGlu: 3.877 ± 0.638
1.899IlePhe: 1.899 ± 0.409
5.064IleGly: 5.064 ± 0.79
1.108IleHis: 1.108 ± 0.348
4.035IleIle: 4.035 ± 0.868
4.035IleLys: 4.035 ± 0.56
3.402IleLeu: 3.402 ± 0.626
0.95IleMet: 0.95 ± 0.258
2.928IleAsn: 2.928 ± 0.477
3.323IlePro: 3.323 ± 0.583
1.662IleGln: 1.662 ± 0.33
4.035IleArg: 4.035 ± 0.456
5.301IleSer: 5.301 ± 0.884
4.194IleThr: 4.194 ± 0.574
3.165IleVal: 3.165 ± 0.591
0.475IleTrp: 0.475 ± 0.152
2.136IleTyr: 2.136 ± 0.34
0.0IleXaa: 0.0 ± 0.0
Lys
5.539LysAla: 5.539 ± 0.755
0.95LysCys: 0.95 ± 0.28
3.956LysAsp: 3.956 ± 0.518
4.352LysGlu: 4.352 ± 0.619
1.978LysPhe: 1.978 ± 0.412
3.956LysGly: 3.956 ± 0.545
0.712LysHis: 0.712 ± 0.264
3.165LysIle: 3.165 ± 0.436
4.985LysLys: 4.985 ± 0.799
6.33LysLeu: 6.33 ± 0.767
2.216LysMet: 2.216 ± 0.477
2.136LysAsn: 2.136 ± 0.462
3.877LysPro: 3.877 ± 0.576
3.165LysGln: 3.165 ± 0.55
5.301LysArg: 5.301 ± 0.672
3.877LysSer: 3.877 ± 0.652
3.956LysThr: 3.956 ± 0.537
3.323LysVal: 3.323 ± 0.512
0.712LysTrp: 0.712 ± 0.255
2.216LysTyr: 2.216 ± 0.423
0.0LysXaa: 0.0 ± 0.0
Leu
7.121LeuAla: 7.121 ± 0.815
0.396LeuCys: 0.396 ± 0.175
3.877LeuAsp: 3.877 ± 0.591
4.985LeuGlu: 4.985 ± 0.61
2.69LeuPhe: 2.69 ± 0.521
4.827LeuGly: 4.827 ± 0.619
1.583LeuHis: 1.583 ± 0.382
6.093LeuIle: 6.093 ± 0.86
6.488LeuLys: 6.488 ± 0.742
7.042LeuLeu: 7.042 ± 0.813
2.374LeuMet: 2.374 ± 0.386
4.589LeuAsn: 4.589 ± 0.61
3.086LeuPro: 3.086 ± 0.505
2.769LeuGln: 2.769 ± 0.45
4.431LeuArg: 4.431 ± 0.483
5.934LeuSer: 5.934 ± 0.622
4.035LeuThr: 4.035 ± 0.559
4.273LeuVal: 4.273 ± 0.533
1.108LeuTrp: 1.108 ± 0.285
2.295LeuTyr: 2.295 ± 0.395
0.0LeuXaa: 0.0 ± 0.0
Met
2.769MetAla: 2.769 ± 0.467
0.237MetCys: 0.237 ± 0.154
1.266MetAsp: 1.266 ± 0.305
1.503MetGlu: 1.503 ± 0.342
0.633MetPhe: 0.633 ± 0.282
1.82MetGly: 1.82 ± 0.372
0.158MetHis: 0.158 ± 0.114
1.503MetIle: 1.503 ± 0.315
2.611MetLys: 2.611 ± 0.515
2.295MetLeu: 2.295 ± 0.388
1.108MetMet: 1.108 ± 0.344
1.741MetAsn: 1.741 ± 0.376
1.187MetPro: 1.187 ± 0.282
1.503MetGln: 1.503 ± 0.356
2.453MetArg: 2.453 ± 0.513
2.532MetSer: 2.532 ± 0.521
2.136MetThr: 2.136 ± 0.418
1.899MetVal: 1.899 ± 0.349
0.237MetTrp: 0.237 ± 0.14
1.029MetTyr: 1.029 ± 0.261
0.0MetXaa: 0.0 ± 0.0
Asn
4.51AsnAla: 4.51 ± 0.759
0.396AsnCys: 0.396 ± 0.18
2.374AsnAsp: 2.374 ± 0.355
3.165AsnGlu: 3.165 ± 0.467
1.108AsnPhe: 1.108 ± 0.252
4.352AsnGly: 4.352 ± 0.5
0.87AsnHis: 0.87 ± 0.241
3.007AsnIle: 3.007 ± 0.531
2.769AsnLys: 2.769 ± 0.554
3.007AsnLeu: 3.007 ± 0.497
1.424AsnMet: 1.424 ± 0.357
2.374AsnAsn: 2.374 ± 0.527
2.69AsnPro: 2.69 ± 0.41
3.086AsnGln: 3.086 ± 0.501
1.82AsnArg: 1.82 ± 0.313
2.611AsnSer: 2.611 ± 0.42
3.165AsnThr: 3.165 ± 0.519
3.323AsnVal: 3.323 ± 0.679
0.475AsnTrp: 0.475 ± 0.174
1.978AsnTyr: 1.978 ± 0.387
0.0AsnXaa: 0.0 ± 0.0
Pro
2.611ProAla: 2.611 ± 0.435
0.158ProCys: 0.158 ± 0.104
3.165ProAsp: 3.165 ± 0.401
4.827ProGlu: 4.827 ± 0.661
1.503ProPhe: 1.503 ± 0.325
2.216ProGly: 2.216 ± 0.407
0.712ProHis: 0.712 ± 0.237
2.453ProIle: 2.453 ± 0.382
2.374ProLys: 2.374 ± 0.362
2.453ProLeu: 2.453 ± 0.428
1.029ProMet: 1.029 ± 0.3
1.583ProAsn: 1.583 ± 0.377
1.108ProPro: 1.108 ± 0.315
1.424ProGln: 1.424 ± 0.271
2.057ProArg: 2.057 ± 0.387
2.611ProSer: 2.611 ± 0.417
1.978ProThr: 1.978 ± 0.304
3.244ProVal: 3.244 ± 0.469
0.554ProTrp: 0.554 ± 0.203
1.029ProTyr: 1.029 ± 0.287
0.0ProXaa: 0.0 ± 0.0
Gln
3.877GlnAla: 3.877 ± 0.595
0.396GlnCys: 0.396 ± 0.166
2.769GlnAsp: 2.769 ± 0.459
2.136GlnGlu: 2.136 ± 0.431
0.95GlnPhe: 0.95 ± 0.233
3.165GlnGly: 3.165 ± 0.544
0.87GlnHis: 0.87 ± 0.25
2.928GlnIle: 2.928 ± 0.439
2.295GlnLys: 2.295 ± 0.536
4.035GlnLeu: 4.035 ± 0.449
1.266GlnMet: 1.266 ± 0.315
1.899GlnAsn: 1.899 ± 0.609
1.662GlnPro: 1.662 ± 0.337
2.849GlnGln: 2.849 ± 0.806
3.402GlnArg: 3.402 ± 0.492
2.295GlnSer: 2.295 ± 0.406
1.583GlnThr: 1.583 ± 0.302
1.82GlnVal: 1.82 ± 0.383
1.266GlnTrp: 1.266 ± 0.294
1.978GlnTyr: 1.978 ± 0.407
0.0GlnXaa: 0.0 ± 0.0
Arg
4.115ArgAla: 4.115 ± 0.437
0.554ArgCys: 0.554 ± 0.203
4.194ArgAsp: 4.194 ± 0.574
4.431ArgGlu: 4.431 ± 0.646
2.295ArgPhe: 2.295 ± 0.487
3.402ArgGly: 3.402 ± 0.476
1.583ArgHis: 1.583 ± 0.307
3.877ArgIle: 3.877 ± 0.618
4.906ArgLys: 4.906 ± 0.735
5.381ArgLeu: 5.381 ± 0.519
1.899ArgMet: 1.899 ± 0.375
2.928ArgAsn: 2.928 ± 0.619
1.741ArgPro: 1.741 ± 0.36
2.769ArgGln: 2.769 ± 0.423
3.719ArgArg: 3.719 ± 0.638
3.402ArgSer: 3.402 ± 0.501
2.057ArgThr: 2.057 ± 0.332
3.086ArgVal: 3.086 ± 0.414
1.187ArgTrp: 1.187 ± 0.385
2.216ArgTyr: 2.216 ± 0.445
0.0ArgXaa: 0.0 ± 0.0
Ser
5.222SerAla: 5.222 ± 0.906
0.87SerCys: 0.87 ± 0.306
3.402SerAsp: 3.402 ± 0.475
3.956SerGlu: 3.956 ± 0.557
2.532SerPhe: 2.532 ± 0.429
5.301SerGly: 5.301 ± 0.845
1.345SerHis: 1.345 ± 0.402
3.561SerIle: 3.561 ± 0.562
3.719SerLys: 3.719 ± 0.596
5.381SerLeu: 5.381 ± 0.577
1.82SerMet: 1.82 ± 0.278
2.849SerAsn: 2.849 ± 0.453
2.69SerPro: 2.69 ± 0.393
3.007SerGln: 3.007 ± 0.488
3.877SerArg: 3.877 ± 0.649
3.561SerSer: 3.561 ± 0.801
2.453SerThr: 2.453 ± 0.556
3.798SerVal: 3.798 ± 0.478
0.95SerTrp: 0.95 ± 0.28
2.374SerTyr: 2.374 ± 0.451
0.0SerXaa: 0.0 ± 0.0
Thr
5.697ThrAla: 5.697 ± 0.729
0.396ThrCys: 0.396 ± 0.159
2.769ThrAsp: 2.769 ± 0.531
2.453ThrGlu: 2.453 ± 0.548
1.266ThrPhe: 1.266 ± 0.306
4.035ThrGly: 4.035 ± 0.609
0.791ThrHis: 0.791 ± 0.229
3.323ThrIle: 3.323 ± 0.528
4.115ThrLys: 4.115 ± 0.698
4.035ThrLeu: 4.035 ± 0.584
1.029ThrMet: 1.029 ± 0.325
2.295ThrAsn: 2.295 ± 0.504
2.69ThrPro: 2.69 ± 0.462
2.216ThrGln: 2.216 ± 0.337
2.136ThrArg: 2.136 ± 0.387
3.244ThrSer: 3.244 ± 0.543
2.374ThrThr: 2.374 ± 0.459
2.611ThrVal: 2.611 ± 0.456
0.791ThrTrp: 0.791 ± 0.253
1.345ThrTyr: 1.345 ± 0.293
0.0ThrXaa: 0.0 ± 0.0
Val
6.093ValAla: 6.093 ± 0.566
0.475ValCys: 0.475 ± 0.192
3.482ValAsp: 3.482 ± 0.518
4.194ValGlu: 4.194 ± 0.468
2.611ValPhe: 2.611 ± 0.427
3.482ValGly: 3.482 ± 0.534
0.554ValHis: 0.554 ± 0.186
3.007ValIle: 3.007 ± 0.531
3.956ValLys: 3.956 ± 0.486
5.46ValLeu: 5.46 ± 0.684
1.503ValMet: 1.503 ± 0.403
3.165ValAsn: 3.165 ± 0.468
1.899ValPro: 1.899 ± 0.415
1.82ValGln: 1.82 ± 0.33
3.086ValArg: 3.086 ± 0.458
3.719ValSer: 3.719 ± 0.529
3.007ValThr: 3.007 ± 0.532
3.877ValVal: 3.877 ± 0.463
0.95ValTrp: 0.95 ± 0.282
1.82ValTyr: 1.82 ± 0.463
0.0ValXaa: 0.0 ± 0.0
Trp
0.791TrpAla: 0.791 ± 0.239
0.554TrpCys: 0.554 ± 0.232
1.187TrpAsp: 1.187 ± 0.253
0.87TrpGlu: 0.87 ± 0.267
0.95TrpPhe: 0.95 ± 0.24
0.712TrpGly: 0.712 ± 0.208
0.317TrpHis: 0.317 ± 0.141
0.712TrpIle: 0.712 ± 0.202
1.424TrpLys: 1.424 ± 0.381
2.532TrpLeu: 2.532 ± 0.57
0.633TrpMet: 0.633 ± 0.191
0.237TrpAsn: 0.237 ± 0.131
0.712TrpPro: 0.712 ± 0.224
0.95TrpGln: 0.95 ± 0.251
0.95TrpArg: 0.95 ± 0.322
0.791TrpSer: 0.791 ± 0.253
0.554TrpThr: 0.554 ± 0.191
1.108TrpVal: 1.108 ± 0.243
0.396TrpTrp: 0.396 ± 0.171
0.396TrpTyr: 0.396 ± 0.168
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.323TyrAla: 3.323 ± 0.48
0.554TyrCys: 0.554 ± 0.212
2.611TyrAsp: 2.611 ± 0.599
1.899TyrGlu: 1.899 ± 0.43
1.424TyrPhe: 1.424 ± 0.338
2.057TyrGly: 2.057 ± 0.496
0.633TyrHis: 0.633 ± 0.246
1.82TyrIle: 1.82 ± 0.515
1.503TyrLys: 1.503 ± 0.334
2.769TyrLeu: 2.769 ± 0.396
0.475TyrMet: 0.475 ± 0.219
1.503TyrAsn: 1.503 ± 0.447
1.662TyrPro: 1.662 ± 0.472
2.216TyrGln: 2.216 ± 0.435
2.295TyrArg: 2.295 ± 0.457
1.503TyrSer: 1.503 ± 0.413
1.583TyrThr: 1.583 ± 0.268
2.136TyrVal: 2.136 ± 0.398
0.791TyrTrp: 0.791 ± 0.228
1.029TyrTyr: 1.029 ± 0.273
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 70 proteins (12639 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski