Candidatus Paraburkholderia kirkii UZHbot1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia; Candidatus Paraburkholderia kirkii

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2015 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G4MG30|G4MG30_9BURK Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase OS=Candidatus Paraburkholderia kirkii UZHbot1 OX=1055526 GN=cobT PE=3 SV=1
MM1 pKa = 7.66SEE3 pKa = 4.05VIEE6 pKa = 4.22YY7 pKa = 10.35KK8 pKa = 10.44SWVCLICGWIYY19 pKa = 10.96NEE21 pKa = 4.19EE22 pKa = 3.96EE23 pKa = 4.17GLPEE27 pKa = 3.98EE28 pKa = 5.44GIAAGTRR35 pKa = 11.84FDD37 pKa = 6.49DD38 pKa = 5.49IPDD41 pKa = 3.44DD42 pKa = 3.99WRR44 pKa = 11.84CPLCDD49 pKa = 3.13VGKK52 pKa = 10.42ADD54 pKa = 4.28FVAVEE59 pKa = 4.2FF60 pKa = 4.64

Molecular weight: 6.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G4M4C3|G4M4C3_9BURK SN-glycerol-3-phosphate transport system permease protein UgpE (TC 3.A.1.1.3) OS=Candidatus Paraburkholderia kirkii UZHbot1 OX=1055526 GN=BKIR_c12_2384 PE=4 SV=1
MM1 pKa = 7.52NPRR4 pKa = 11.84IAIGFTRR11 pKa = 11.84VASHH15 pKa = 7.13AARR18 pKa = 11.84PSRR21 pKa = 11.84LGMVRR26 pKa = 11.84ALRR29 pKa = 11.84HH30 pKa = 5.13HH31 pKa = 6.06SARR34 pKa = 11.84SQLLAAQLKK43 pKa = 9.23NVHH46 pKa = 6.43PVDD49 pKa = 5.72GIRR52 pKa = 11.84SWFRR56 pKa = 11.84GFFFNLRR63 pKa = 11.84VRR65 pKa = 11.84AASRR69 pKa = 11.84RR70 pKa = 11.84LSLRR74 pKa = 11.84ALLRR78 pKa = 11.84RR79 pKa = 11.84PMQLRR84 pKa = 11.84APSPKK89 pKa = 9.51PKK91 pKa = 10.25AAALTSRR98 pKa = 11.84RR99 pKa = 11.84PRR101 pKa = 11.84RR102 pKa = 11.84LSTSSAGWYY111 pKa = 10.26AFAARR116 pKa = 4.42

Molecular weight: 13.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2015

0

2015

596368

37

1932

296.0

32.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.883 ± 0.09

1.033 ± 0.02

5.537 ± 0.04

5.674 ± 0.052

3.752 ± 0.036

7.776 ± 0.046

2.431 ± 0.026

5.035 ± 0.039

3.874 ± 0.053

9.777 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.51 ± 0.026

2.977 ± 0.034

4.996 ± 0.039

3.541 ± 0.034

7.149 ± 0.068

5.639 ± 0.037

5.247 ± 0.031

7.519 ± 0.046

1.26 ± 0.022

2.39 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski