Natrinema altunense JCM 12890
Average proteome isoelectric point is 4.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3732 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L9ZLR5|L9ZLR5_9EURY Glucose 1-dehydrogenase OS=Natrinema altunense JCM 12890 OX=1227494 GN=gdh PE=3 SV=1
MM1 pKa = 7.72 LDD3 pKa = 3.47 VGDD6 pKa = 4.25 EE7 pKa = 4.08 APEE10 pKa = 4.12 FEE12 pKa = 5.06 LQNQHH17 pKa = 5.8 GEE19 pKa = 4.23 TVRR22 pKa = 11.84 RR23 pKa = 11.84 SDD25 pKa = 3.9 FEE27 pKa = 4.02 GQRR30 pKa = 11.84 LVVYY34 pKa = 7.12 FYY36 pKa = 10.88 PRR38 pKa = 11.84 ANTDD42 pKa = 2.45 GCTTEE47 pKa = 4.57 ACGFNDD53 pKa = 3.85 TLPQFEE59 pKa = 4.41 AHH61 pKa = 6.71 DD62 pKa = 3.89 VAVVGISDD70 pKa = 5.25 DD71 pKa = 4.02 PVDD74 pKa = 5.88 DD75 pKa = 4.28 IADD78 pKa = 4.39 FAADD82 pKa = 3.57 YY83 pKa = 11.16 DD84 pKa = 4.58 LEE86 pKa = 5.8 FDD88 pKa = 4.43 LLSDD92 pKa = 3.7 EE93 pKa = 4.68 FGEE96 pKa = 4.68 VATLYY101 pKa = 10.78 DD102 pKa = 3.95 SYY104 pKa = 11.76 GEE106 pKa = 3.91 KK107 pKa = 10.8 QMFGNTFDD115 pKa = 3.69 GVFRR119 pKa = 11.84 NTYY122 pKa = 9.66 VVGPDD127 pKa = 3.3 GRR129 pKa = 11.84 IEE131 pKa = 3.71 AAYY134 pKa = 10.04 EE135 pKa = 4.17 GVSPDD140 pKa = 3.09 GHH142 pKa = 7.2 ADD144 pKa = 3.3 TVLADD149 pKa = 4.38 LVPADD154 pKa = 3.72 VTHH157 pKa = 7.12
Molecular weight: 17.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.695
IPC2_protein 3.694
IPC_protein 3.706
Toseland 3.49
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.401
Solomon 3.694
Lehninger 3.656
Nozaki 3.821
DTASelect 4.062
Thurlkill 3.541
EMBOSS 3.656
Sillero 3.834
Patrickios 1.062
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.751
Protein with the highest isoelectric point:
>tr|L9ZES4|L9ZES4_9EURY Uncharacterized protein OS=Natrinema altunense JCM 12890 OX=1227494 GN=C485_18112 PE=4 SV=1
MM1 pKa = 7.51 LKK3 pKa = 9.99 SCLFRR8 pKa = 11.84 VSIDD12 pKa = 3.03 ATTADD17 pKa = 4.17 PPAAGSKK24 pKa = 9.47 FLPRR28 pKa = 11.84 AVGEE32 pKa = 4.22 VRR34 pKa = 11.84 HH35 pKa = 5.71 MLRR38 pKa = 11.84 EE39 pKa = 3.92 LLIGFGVVEE48 pKa = 4.1 ILKK51 pKa = 9.67 PEE53 pKa = 4.76 PIIRR57 pKa = 11.84 LCEE60 pKa = 4.21 RR61 pKa = 11.84 IGLRR65 pKa = 11.84 NPGKK69 pKa = 8.22 TEE71 pKa = 3.75 RR72 pKa = 11.84 RR73 pKa = 11.84 PLALTGARR81 pKa = 11.84 LEE83 pKa = 4.16 GLVFVWLLVRR93 pKa = 11.84 GRR95 pKa = 11.84 QGSTLVSALLGAAGLALVLVPRR117 pKa = 11.84 PIIEE121 pKa = 4.52 LSQHH125 pKa = 5.54 LVYY128 pKa = 11.09 AEE130 pKa = 3.91 TDD132 pKa = 3.43 RR133 pKa = 11.84 LEE135 pKa = 4.21 LQPWVVPAARR145 pKa = 11.84 LLGVLYY151 pKa = 8.97 LTVTLLSQRR160 pKa = 11.84 PGTEE164 pKa = 3.83 TTDD167 pKa = 3.46 EE168 pKa = 4.6 DD169 pKa = 4.49 RR170 pKa = 11.84 DD171 pKa = 3.84 GADD174 pKa = 3.25 RR175 pKa = 11.84 SPRR178 pKa = 11.84 WRR180 pKa = 11.84 RR181 pKa = 11.84 RR182 pKa = 11.84 RR183 pKa = 11.84 SGG185 pKa = 3.06
Molecular weight: 20.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 9.355
IPC_protein 10.365
Toseland 10.599
ProMoST 10.423
Dawson 10.672
Bjellqvist 10.452
Wikipedia 10.935
Rodwell 10.657
Grimsley 10.716
Solomon 10.877
Lehninger 10.833
Nozaki 10.599
DTASelect 10.438
Thurlkill 10.599
EMBOSS 11.023
Sillero 10.628
Patrickios 10.423
IPC_peptide 10.877
IPC2_peptide 9.677
IPC2.peptide.svr19 8.69
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3732
0
3732
1035287
28
1725
277.4
30.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.991 ± 0.063
0.757 ± 0.012
8.707 ± 0.052
8.61 ± 0.054
3.21 ± 0.026
8.42 ± 0.041
1.982 ± 0.02
4.366 ± 0.031
1.719 ± 0.029
8.87 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.723 ± 0.018
2.241 ± 0.024
4.668 ± 0.029
2.355 ± 0.025
6.751 ± 0.042
5.52 ± 0.029
6.629 ± 0.031
8.658 ± 0.04
1.118 ± 0.016
2.704 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here