Pandoravirus inopinatum
Average proteome isoelectric point is 7.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1839 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B5IYE6|A0A0B5IYE6_9VIRU Uncharacterized protein OS=Pandoravirus inopinatum OX=1605721 PE=4 SV=1
MM1 pKa = 7.02 TALGKK6 pKa = 10.46 RR7 pKa = 11.84 ILEE10 pKa = 4.1 IVSTRR15 pKa = 11.84 VEE17 pKa = 4.58 DD18 pKa = 4.84 DD19 pKa = 4.12 DD20 pKa = 4.1 GTPAVPLAKK29 pKa = 9.93 RR30 pKa = 11.84 PRR32 pKa = 11.84 CDD34 pKa = 3.34 NMPEE38 pKa = 4.18 PGCAGIAVGDD48 pKa = 3.55 TWNGIEE54 pKa = 4.69 CDD56 pKa = 3.81 EE57 pKa = 4.74 EE58 pKa = 4.51 DD59 pKa = 4.41 VEE61 pKa = 4.61 ARR63 pKa = 11.84 DD64 pKa = 4.1 HH65 pKa = 6.03 IVEE68 pKa = 4.22 VVLAGGALATVLDD81 pKa = 4.23 QASCHH86 pKa = 6.51 HH87 pKa = 7.13 IDD89 pKa = 4.07 LALIQLFDD97 pKa = 4.43 DD98 pKa = 4.06 CTALARR104 pKa = 11.84 LCRR107 pKa = 11.84 AIAPSLPQALPAVLAADD124 pKa = 4.09 SEE126 pKa = 4.49 PDD128 pKa = 2.93 KK129 pKa = 10.91 TVYY132 pKa = 10.38 HH133 pKa = 5.02 VTIDD137 pKa = 3.76 ALLCRR142 pKa = 11.84 AAQQGRR148 pKa = 11.84 PAVLAVLMRR157 pKa = 11.84 HH158 pKa = 6.01 LADD161 pKa = 4.24 EE162 pKa = 4.44 YY163 pKa = 11.35 DD164 pKa = 3.49 VARR167 pKa = 11.84 ALEE170 pKa = 4.2 MADD173 pKa = 4.7 EE174 pKa = 4.71 EE175 pKa = 4.97 DD176 pKa = 4.27 DD177 pKa = 3.86 PAAIEE182 pKa = 4.99 LIAEE186 pKa = 4.49 TYY188 pKa = 10.41 EE189 pKa = 4.09 KK190 pKa = 10.87 DD191 pKa = 3.58 AGKK194 pKa = 10.34 DD195 pKa = 3.27 DD196 pKa = 3.79 TGEE199 pKa = 4.72 DD200 pKa = 3.67 DD201 pKa = 4.04 TGEE204 pKa = 4.77 DD205 pKa = 3.68 DD206 pKa = 3.75 TGEE209 pKa = 4.69 DD210 pKa = 3.38 EE211 pKa = 4.4 TGSCRR216 pKa = 11.84 RR217 pKa = 11.84 IAYY220 pKa = 8.84 VALTCAVHH228 pKa = 6.23 SSRR231 pKa = 11.84 VALIHH236 pKa = 6.86 RR237 pKa = 11.84 LVDD240 pKa = 3.14 KK241 pKa = 11.17 CDD243 pKa = 3.4 VEE245 pKa = 5.08 DD246 pKa = 3.67 VHH248 pKa = 8.1 GLLDD252 pKa = 4.45 DD253 pKa = 4.32 CLEE256 pKa = 4.9 DD257 pKa = 4.78 GDD259 pKa = 4.59 VFAALWQHH267 pKa = 6.65 AVLCAHH273 pKa = 7.62 AYY275 pKa = 8.98 AASLPPCPALDD286 pKa = 3.72 HH287 pKa = 6.35 LTLQIANGEE296 pKa = 4.19 PCADD300 pKa = 3.17 MCASYY305 pKa = 10.99 EE306 pKa = 4.15 PADD309 pKa = 4.17 SDD311 pKa = 5.13 NDD313 pKa = 3.99 TDD315 pKa = 6.49 DD316 pKa = 5.31 DD317 pKa = 4.39 TDD319 pKa = 5.77 DD320 pKa = 6.2 DD321 pKa = 5.5 DD322 pKa = 7.18 GGDD325 pKa = 4.1 NGNDD329 pKa = 4.38 DD330 pKa = 5.53 DD331 pKa = 6.73 DD332 pKa = 6.51 NDD334 pKa = 4.33 DD335 pKa = 4.17 ADD337 pKa = 4.06 QGSHH341 pKa = 5.42 EE342 pKa = 4.23 QAQQ345 pKa = 3.42
Molecular weight: 36.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.8
IPC2_protein 3.821
IPC_protein 3.872
Toseland 3.643
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.808
Rodwell 3.694
Grimsley 3.541
Solomon 3.859
Lehninger 3.821
Nozaki 3.973
DTASelect 4.24
Thurlkill 3.694
EMBOSS 3.821
Sillero 3.986
Patrickios 2.359
IPC_peptide 3.859
IPC2_peptide 3.961
IPC2.peptide.svr19 3.884
Protein with the highest isoelectric point:
>tr|A0A0B5J4K2|A0A0B5J4K2_9VIRU Uncharacterized protein OS=Pandoravirus inopinatum OX=1605721 PE=4 SV=1
MM1 pKa = 7.09 TWFFATVAGGGLGVGGRR18 pKa = 11.84 AAGVRR23 pKa = 11.84 PLCRR27 pKa = 11.84 AVILALCRR35 pKa = 11.84 QTRR38 pKa = 11.84 IRR40 pKa = 11.84 RR41 pKa = 11.84 VSRR44 pKa = 11.84 ADD46 pKa = 3.57 FAPSSPLLIFFPIGHH61 pKa = 7.73 LRR63 pKa = 11.84 RR64 pKa = 11.84 QTRR67 pKa = 11.84 VWSRR71 pKa = 11.84 LSTATVSFPFRR82 pKa = 11.84 LFFQTLPFSNWAAVVAHH99 pKa = 6.98 APPAEE104 pKa = 4.22 ATQKK108 pKa = 8.9 VAKK111 pKa = 10.49 NFFGRR116 pKa = 11.84 FAMIIFPFFFLIGGGAFSWGRR137 pKa = 11.84 RR138 pKa = 11.84 RR139 pKa = 11.84 QKK141 pKa = 8.44 STSRR145 pKa = 11.84 RR146 pKa = 11.84 RR147 pKa = 11.84 VLPFAWPTPRR157 pKa = 11.84 AAFVPFALQPSKK169 pKa = 10.79 INRR172 pKa = 11.84 FGAWHH177 pKa = 6.11 MSKK180 pKa = 8.5 ITSNIANGLPLARR193 pKa = 11.84 LKK195 pKa = 10.43 AQCRR199 pKa = 11.84 QATFFLFKK207 pKa = 9.74 WKK209 pKa = 9.73 HH210 pKa = 4.24 ARR212 pKa = 11.84 KK213 pKa = 9.37 KK214 pKa = 10.04 KK215 pKa = 9.53 RR216 pKa = 11.84 PARR219 pKa = 11.84 RR220 pKa = 11.84 RR221 pKa = 11.84 LL222 pKa = 3.29
Molecular weight: 25.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.389
IPC2_protein 10.833
IPC_protein 12.427
Toseland 12.588
ProMoST 13.086
Dawson 12.588
Bjellqvist 12.588
Wikipedia 13.071
Rodwell 12.252
Grimsley 12.632
Solomon 13.086
Lehninger 12.983
Nozaki 12.588
DTASelect 12.588
Thurlkill 12.588
EMBOSS 13.086
Sillero 12.588
Patrickios 11.959
IPC_peptide 13.086
IPC2_peptide 12.076
IPC2.peptide.svr19 9.116
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1839
0
1839
542761
99
1504
295.1
32.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.172 ± 0.083
2.948 ± 0.044
6.738 ± 0.066
3.481 ± 0.039
2.969 ± 0.047
7.894 ± 0.065
3.177 ± 0.039
3.154 ± 0.027
2.566 ± 0.046
8.146 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.056 ± 0.022
2.173 ± 0.028
6.652 ± 0.054
2.736 ± 0.034
9.044 ± 0.059
6.03 ± 0.057
5.852 ± 0.051
7.11 ± 0.039
2.122 ± 0.036
1.978 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here