Moraxella phage Mcat7
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R6PHF1|A0A0R6PHF1_9CAUD Uncharacterized protein OS=Moraxella phage Mcat7 OX=1647553 PE=4 SV=1
MM1 pKa = 7.2 SQFATLDD8 pKa = 3.58 EE9 pKa = 4.72 VKK11 pKa = 10.53 HH12 pKa = 5.6 HH13 pKa = 6.48 LRR15 pKa = 11.84 YY16 pKa = 10.36 DD17 pKa = 3.95 DD18 pKa = 5.29 DD19 pKa = 6.58 DD20 pKa = 6.33 SDD22 pKa = 5.7 GILQIYY28 pKa = 8.15 LQSAQTAVKK37 pKa = 10.57 NYY39 pKa = 8.2 ITDD42 pKa = 5.22 DD43 pKa = 3.43 ITDD46 pKa = 3.46 EE47 pKa = 4.35 MLPSLKK53 pKa = 10.46 VATLLLVGYY62 pKa = 10.57 LDD64 pKa = 4.51 DD65 pKa = 4.01 NRR67 pKa = 11.84 NGEE70 pKa = 4.11 RR71 pKa = 11.84 GAEE74 pKa = 3.94 FGNFLPAPVRR84 pKa = 11.84 QMLAPYY90 pKa = 7.83 RR91 pKa = 11.84 TPTFF95 pKa = 3.48
Molecular weight: 10.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.418
IPC2_protein 4.418
IPC_protein 4.329
Toseland 4.126
ProMoST 4.495
Dawson 4.317
Bjellqvist 4.469
Wikipedia 4.266
Rodwell 4.164
Grimsley 4.037
Solomon 4.317
Lehninger 4.266
Nozaki 4.444
DTASelect 4.698
Thurlkill 4.177
EMBOSS 4.279
Sillero 4.457
Patrickios 3.719
IPC_peptide 4.317
IPC2_peptide 4.431
IPC2.peptide.svr19 4.406
Protein with the highest isoelectric point:
>tr|A0A0R6PHK5|A0A0R6PHK5_9CAUD Uncharacterized protein OS=Moraxella phage Mcat7 OX=1647553 PE=4 SV=1
MM1 pKa = 7.77 AKK3 pKa = 10.19 YY4 pKa = 9.78 KK5 pKa = 10.92 LPGLQPRR12 pKa = 11.84 LKK14 pKa = 10.1 PLTSHH19 pKa = 5.26 QSKK22 pKa = 10.67 RR23 pKa = 11.84 NWGQGRR29 pKa = 11.84 GGRR32 pKa = 11.84 PWRR35 pKa = 11.84 RR36 pKa = 11.84 LRR38 pKa = 11.84 AEE40 pKa = 3.5 ILARR44 pKa = 11.84 DD45 pKa = 3.97 QYY47 pKa = 9.74 TCRR50 pKa = 11.84 HH51 pKa = 6.44 CGRR54 pKa = 11.84 IGGQLEE60 pKa = 3.87 VDD62 pKa = 4.52 HH63 pKa = 7.27 IINTAQGGTDD73 pKa = 4.16 DD74 pKa = 4.08 KK75 pKa = 11.7 TNLQTLCRR83 pKa = 11.84 DD84 pKa = 3.57 CHH86 pKa = 4.28 QTKK89 pKa = 8.26 TQAEE93 pKa = 4.12 ARR95 pKa = 11.84 VGGVQQFFF103 pKa = 3.59
Molecular weight: 11.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.316
IPC2_protein 9.399
IPC_protein 9.736
Toseland 10.438
ProMoST 10.043
Dawson 10.54
Bjellqvist 10.233
Wikipedia 10.701
Rodwell 10.847
Grimsley 10.584
Solomon 10.613
Lehninger 10.599
Nozaki 10.482
DTASelect 10.204
Thurlkill 10.438
EMBOSS 10.833
Sillero 10.482
Patrickios 10.628
IPC_peptide 10.628
IPC2_peptide 9.428
IPC2.peptide.svr19 8.403
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
47
0
47
11582
40
1614
246.4
27.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.591 ± 0.58
0.898 ± 0.202
6.096 ± 0.219
5.232 ± 0.29
3.298 ± 0.193
6.381 ± 0.385
2.409 ± 0.301
6.968 ± 0.255
6.925 ± 0.363
8.28 ± 0.266
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.366 ± 0.252
5.137 ± 0.308
3.445 ± 0.314
4.801 ± 0.239
4.706 ± 0.246
7.089 ± 0.623
6.7 ± 0.48
6.018 ± 0.238
1.373 ± 0.157
3.29 ± 0.25
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here