Sulfolobus spindle-shaped virus 7

Taxonomy: Viruses; Fuselloviridae; Alphafusellovirus

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 33 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D1GF71|D1GF71_9VIRU CopG-like RHH protein OS=Sulfolobus spindle-shaped virus 7 OX=693628 PE=4 SV=1
MM1 pKa = 7.43SNEE4 pKa = 3.71LRR6 pKa = 11.84YY7 pKa = 9.63IKK9 pKa = 10.39QKK11 pKa = 10.77LDD13 pKa = 3.52EE14 pKa = 4.96LLSEE18 pKa = 5.22CICDD22 pKa = 3.84DD23 pKa = 4.05PEE25 pKa = 4.93SMRR28 pKa = 11.84EE29 pKa = 4.0LEE31 pKa = 4.73EE32 pKa = 3.76MMKK35 pKa = 9.82MIEE38 pKa = 4.35HH39 pKa = 6.63ALEE42 pKa = 4.17YY43 pKa = 10.44CQVGDD48 pKa = 3.45WW49 pKa = 3.6

Molecular weight: 5.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D1GF54|D1GF54_9VIRU Uncharacterized protein OS=Sulfolobus spindle-shaped virus 7 OX=693628 PE=4 SV=1
MM1 pKa = 6.83MRR3 pKa = 11.84WMTNGLVSPKK13 pKa = 10.23NIRR16 pKa = 11.84RR17 pKa = 11.84NNPRR21 pKa = 11.84FRR23 pKa = 11.84FNYY26 pKa = 10.01ADD28 pKa = 3.33YY29 pKa = 10.91CFAVQKK35 pKa = 9.53TKK37 pKa = 11.08AQIRR41 pKa = 11.84EE42 pKa = 4.4INTKK46 pKa = 7.77PQIYY50 pKa = 9.24RR51 pKa = 11.84VLSSHH56 pKa = 7.03LSYY59 pKa = 10.91HH60 pKa = 5.69RR61 pKa = 11.84LSRR64 pKa = 11.84KK65 pKa = 8.38SFCFISIFDD74 pKa = 4.13RR75 pKa = 11.84LLHH78 pKa = 6.33GSVPQQ83 pKa = 3.58

Molecular weight: 9.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

33

0

33

5848

49

811

177.2

20.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.549 ± 0.427

0.718 ± 0.193

3.608 ± 0.542

6.019 ± 0.981

4.634 ± 0.353

6.156 ± 0.477

1.3 ± 0.283

8.567 ± 0.342

7.353 ± 0.896

10.311 ± 0.45

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.445 ± 0.267

5.301 ± 0.54

3.54 ± 0.334

3.232 ± 0.298

4.036 ± 0.624

7.062 ± 0.602

6.908 ± 0.65

7.302 ± 0.376

1.488 ± 0.15

5.472 ± 0.508
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski