Flavobacteriaceae bacterium (strain 3519-10)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; unclassified Flavobacteriaceae

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2533 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6X6H7|C6X6H7_FLAB3 Peptide chain release factor 3 OS=Flavobacteriaceae bacterium (strain 3519-10) OX=531844 GN=prfC PE=3 SV=1
MM1 pKa = 7.56QDD3 pKa = 2.72ITLKK7 pKa = 10.66IIDD10 pKa = 4.39RR11 pKa = 11.84NGQTHH16 pKa = 5.95EE17 pKa = 4.48VVAPTDD23 pKa = 3.19MSMNLMEE30 pKa = 4.84VIRR33 pKa = 11.84SYY35 pKa = 11.43EE36 pKa = 3.86LADD39 pKa = 3.65EE40 pKa = 4.45GTIGVCGGMAMCASCQVYY58 pKa = 9.08VVEE61 pKa = 5.03GSEE64 pKa = 4.25KK65 pKa = 9.22LTGMGDD71 pKa = 3.43EE72 pKa = 4.26EE73 pKa = 5.0DD74 pKa = 4.4AMLSEE79 pKa = 4.71AFHH82 pKa = 6.02VQDD85 pKa = 4.44NSRR88 pKa = 11.84LGCQIHH94 pKa = 5.33VTAEE98 pKa = 3.67IDD100 pKa = 3.61GLEE103 pKa = 4.19VEE105 pKa = 4.58IAPYY109 pKa = 9.53PP110 pKa = 3.68

Molecular weight: 11.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6X2E8|C6X2E8_FLAB3 Aspartate aminotransferase OS=Flavobacteriaceae bacterium (strain 3519-10) OX=531844 GN=FIC_02175 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPSEE10 pKa = 3.59RR11 pKa = 11.84KK12 pKa = 9.33RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 10.03HH17 pKa = 3.97GFRR20 pKa = 11.84EE21 pKa = 4.33RR22 pKa = 11.84MSTPNGRR29 pKa = 11.84RR30 pKa = 11.84VLAARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.18GRR40 pKa = 11.84RR41 pKa = 11.84SLSISGARR49 pKa = 11.84AKK51 pKa = 10.48RR52 pKa = 3.4

Molecular weight: 6.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2533

0

2533

832499

30

2407

328.7

36.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.054 ± 0.047

0.727 ± 0.015

5.442 ± 0.035

6.466 ± 0.057

5.44 ± 0.04

6.575 ± 0.049

1.768 ± 0.022

7.306 ± 0.048

7.416 ± 0.052

9.141 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.462 ± 0.025

5.914 ± 0.046

3.571 ± 0.028

3.529 ± 0.028

3.765 ± 0.035

6.436 ± 0.045

5.717 ± 0.061

6.318 ± 0.037

1.031 ± 0.016

3.92 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski