Amino acid dipepetide frequency for Flavobacteriaceae bacterium (strain 3519-10)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.873AlaAla: 5.873 ± 0.119	0.525AlaCys: 0.525 ± 0.025	4.334AlaAsp: 4.334 ± 0.082	5.23AlaGlu: 5.23 ± 0.094	3.372AlaPhe: 3.372 ± 0.061	5.195AlaGly: 5.195 ± 0.089	1.19AlaHis: 1.19 ± 0.043	4.44AlaIle: 4.44 ± 0.083	4.977AlaLys: 4.977 ± 0.102	6.428AlaLeu: 6.428 ± 0.105	1.771AlaMet: 1.771 ± 0.049	3.587AlaAsn: 3.587 ± 0.074	2.142AlaPro: 2.142 ± 0.06	2.907AlaGln: 2.907 ± 0.058	2.447AlaArg: 2.447 ± 0.063	3.995AlaSer: 3.995 ± 0.082	3.974AlaThr: 3.974 ± 0.087	5.184AlaVal: 5.184 ± 0.086	0.593AlaTrp: 0.593 ± 0.026	2.382AlaTyr: 2.382 ± 0.06	0.0AlaXaa: 0.0 ± 0.0
Cys	0.517CysAla: 0.517 ± 0.03	0.091CysCys: 0.091 ± 0.011	0.418CysAsp: 0.418 ± 0.024	0.466CysGlu: 0.466 ± 0.023	0.414CysPhe: 0.414 ± 0.022	0.682CysGly: 0.682 ± 0.032	0.154CysHis: 0.154 ± 0.015	0.545CysIle: 0.545 ± 0.027	0.389CysLys: 0.389 ± 0.025	0.64CysLeu: 0.64 ± 0.032	0.151CysMet: 0.151 ± 0.014	0.32CysAsn: 0.32 ± 0.018	0.32CysPro: 0.32 ± 0.024	0.165CysGln: 0.165 ± 0.015	0.287CysArg: 0.287 ± 0.017	0.524CysSer: 0.524 ± 0.029	0.423CysThr: 0.423 ± 0.023	0.471CysVal: 0.471 ± 0.023	0.053CysTrp: 0.053 ± 0.008	0.244CysTyr: 0.244 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	3.99AspAla: 3.99 ± 0.077	0.371AspCys: 0.371 ± 0.022	2.864AspAsp: 2.864 ± 0.067	4.201AspGlu: 4.201 ± 0.083	3.868AspPhe: 3.868 ± 0.063	3.777AspGly: 3.777 ± 0.074	1.041AspHis: 1.041 ± 0.037	3.798AspIle: 3.798 ± 0.08	3.672AspLys: 3.672 ± 0.082	5.24AspLeu: 5.24 ± 0.073	1.062AspMet: 1.062 ± 0.038	2.503AspAsn: 2.503 ± 0.057	1.798AspPro: 1.798 ± 0.051	1.754AspGln: 1.754 ± 0.049	2.247AspArg: 2.247 ± 0.051	3.058AspSer: 3.058 ± 0.057	2.362AspThr: 2.362 ± 0.061	3.522AspVal: 3.522 ± 0.071	0.664AspTrp: 0.664 ± 0.025	2.629AspTyr: 2.629 ± 0.051	0.0AspXaa: 0.0 ± 0.0
Glu	4.142GluAla: 4.142 ± 0.093	0.302GluCys: 0.302 ± 0.02	3.325GluAsp: 3.325 ± 0.069	4.502GluGlu: 4.502 ± 0.099	3.238GluPhe: 3.238 ± 0.064	3.492GluGly: 3.492 ± 0.074	1.108GluHis: 1.108 ± 0.037	5.73GluIle: 5.73 ± 0.098	6.034GluLys: 6.034 ± 0.121	5.641GluLeu: 5.641 ± 0.094	1.841GluMet: 1.841 ± 0.047	4.901GluAsn: 4.901 ± 0.086	1.619GluPro: 1.619 ± 0.048	2.256GluGln: 2.256 ± 0.057	2.503GluArg: 2.503 ± 0.058	3.288GluSer: 3.288 ± 0.065	3.701GluThr: 3.701 ± 0.068	4.117GluVal: 4.117 ± 0.083	0.65GluTrp: 0.65 ± 0.03	2.268GluTyr: 2.268 ± 0.051	0.0GluXaa: 0.0 ± 0.0
Phe	3.91PheAla: 3.91 ± 0.074	0.494PheCys: 0.494 ± 0.026	3.038PheAsp: 3.038 ± 0.07	3.314PheGlu: 3.314 ± 0.076	2.945PhePhe: 2.945 ± 0.085	4.012PheGly: 4.012 ± 0.078	0.926PheHis: 0.926 ± 0.035	3.705PheIle: 3.705 ± 0.078	3.278PheLys: 3.278 ± 0.062	5.028PheLeu: 5.028 ± 0.1	1.278PheMet: 1.278 ± 0.039	2.954PheAsn: 2.954 ± 0.078	1.999PhePro: 1.999 ± 0.05	1.583PheGln: 1.583 ± 0.045	2.22PheArg: 2.22 ± 0.048	4.34PheSer: 4.34 ± 0.08	3.23PheThr: 3.23 ± 0.064	3.237PheVal: 3.237 ± 0.071	0.652PheTrp: 0.652 ± 0.033	2.258PheTyr: 2.258 ± 0.057	0.0PheXaa: 0.0 ± 0.0
Gly	4.32GlyAla: 4.32 ± 0.087	0.532GlyCys: 0.532 ± 0.032	3.401GlyAsp: 3.401 ± 0.068	3.672GlyGlu: 3.672 ± 0.067	3.73GlyPhe: 3.73 ± 0.087	4.673GlyGly: 4.673 ± 0.111	1.029GlyHis: 1.029 ± 0.036	5.351GlyIle: 5.351 ± 0.102	5.422GlyLys: 5.422 ± 0.091	5.815GlyLeu: 5.815 ± 0.088	1.691GlyMet: 1.691 ± 0.052	3.942GlyAsn: 3.942 ± 0.092	1.372GlyPro: 1.372 ± 0.037	1.989GlyGln: 1.989 ± 0.05	2.425GlyArg: 2.425 ± 0.051	4.394GlySer: 4.394 ± 0.084	4.262GlyThr: 4.262 ± 0.096	4.205GlyVal: 4.205 ± 0.084	0.759GlyTrp: 0.759 ± 0.029	2.766GlyTyr: 2.766 ± 0.058	0.0GlyXaa: 0.0 ± 0.0
His	1.083HisAla: 1.083 ± 0.036	0.192HisCys: 0.192 ± 0.016	0.897HisAsp: 0.897 ± 0.033	0.989HisGlu: 0.989 ± 0.037	1.19HisPhe: 1.19 ± 0.043	1.07HisGly: 1.07 ± 0.043	0.541HisHis: 0.541 ± 0.03	1.241HisIle: 1.241 ± 0.042	1.037HisLys: 1.037 ± 0.039	1.828HisLeu: 1.828 ± 0.047	0.33HisMet: 0.33 ± 0.021	0.873HisAsn: 0.873 ± 0.031	0.925HisPro: 0.925 ± 0.032	0.73HisGln: 0.73 ± 0.035	0.769HisArg: 0.769 ± 0.029	1.21HisSer: 1.21 ± 0.037	0.926HisThr: 0.926 ± 0.036	0.832HisVal: 0.832 ± 0.037	0.219HisTrp: 0.219 ± 0.016	0.795HisTyr: 0.795 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	5.26IleAla: 5.26 ± 0.089	0.611IleCys: 0.611 ± 0.025	4.184IleAsp: 4.184 ± 0.064	4.565IleGlu: 4.565 ± 0.084	3.986IlePhe: 3.986 ± 0.082	4.65IleGly: 4.65 ± 0.081	1.246IleHis: 1.246 ± 0.041	5.416IleIle: 5.416 ± 0.107	4.921IleLys: 4.921 ± 0.076	6.999IleLeu: 6.999 ± 0.104	1.473IleMet: 1.473 ± 0.044	4.006IleAsn: 4.006 ± 0.082	3.086IlePro: 3.086 ± 0.067	2.286IleGln: 2.286 ± 0.055	2.705IleArg: 2.705 ± 0.068	5.731IleSer: 5.731 ± 0.088	4.36IleThr: 4.36 ± 0.07	4.191IleVal: 4.191 ± 0.073	0.669IleTrp: 0.669 ± 0.028	2.714IleTyr: 2.714 ± 0.062	0.0IleXaa: 0.0 ± 0.0
Lys	4.603LysAla: 4.603 ± 0.088	0.358LysCys: 0.358 ± 0.021	4.167LysAsp: 4.167 ± 0.075	4.925LysGlu: 4.925 ± 0.095	3.562LysPhe: 3.562 ± 0.07	3.893LysGly: 3.893 ± 0.075	1.25LysHis: 1.25 ± 0.038	6.647LysIle: 6.647 ± 0.086	6.131LysLys: 6.131 ± 0.104	6.186LysLeu: 6.186 ± 0.095	2.299LysMet: 2.299 ± 0.056	5.443LysAsn: 5.443 ± 0.083	2.613LysPro: 2.613 ± 0.065	2.282LysGln: 2.282 ± 0.06	2.452LysArg: 2.452 ± 0.056	4.402LysSer: 4.402 ± 0.071	4.732LysThr: 4.732 ± 0.083	4.61LysVal: 4.61 ± 0.076	0.68LysTrp: 0.68 ± 0.03	2.92LysTyr: 2.92 ± 0.063	0.0LysXaa: 0.0 ± 0.0
Leu	6.517LeuAla: 6.517 ± 0.093	0.668LeuCys: 0.668 ± 0.029	4.688LeuAsp: 4.688 ± 0.075	5.661LeuGlu: 5.661 ± 0.087	4.683LeuPhe: 4.683 ± 0.1	5.808LeuGly: 5.808 ± 0.097	1.661LeuHis: 1.661 ± 0.047	6.316LeuIle: 6.316 ± 0.107	7.596LeuLys: 7.596 ± 0.105	8.443LeuLeu: 8.443 ± 0.145	2.465LeuMet: 2.465 ± 0.059	5.582LeuAsn: 5.582 ± 0.1	3.745LeuPro: 3.745 ± 0.076	3.463LeuGln: 3.463 ± 0.07	3.576LeuArg: 3.576 ± 0.07	6.354LeuSer: 6.354 ± 0.09	5.071LeuThr: 5.071 ± 0.074	5.258LeuVal: 5.258 ± 0.092	0.77LeuTrp: 0.77 ± 0.034	3.085LeuTyr: 3.085 ± 0.067	0.0LeuXaa: 0.0 ± 0.0
Met	1.804MetAla: 1.804 ± 0.048	0.159MetCys: 0.159 ± 0.014	1.262MetAsp: 1.262 ± 0.046	1.458MetGlu: 1.458 ± 0.038	0.998MetPhe: 0.998 ± 0.039	1.514MetGly: 1.514 ± 0.042	0.455MetHis: 0.455 ± 0.025	1.739MetIle: 1.739 ± 0.047	2.494MetLys: 2.494 ± 0.046	2.192MetLeu: 2.192 ± 0.057	0.846MetMet: 0.846 ± 0.035	1.505MetAsn: 1.505 ± 0.044	1.011MetPro: 1.011 ± 0.036	0.983MetGln: 0.983 ± 0.036	0.968MetArg: 0.968 ± 0.03	1.487MetSer: 1.487 ± 0.038	1.341MetThr: 1.341 ± 0.038	1.489MetVal: 1.489 ± 0.048	0.203MetTrp: 0.203 ± 0.017	0.716MetTyr: 0.716 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	4.159AsnAla: 4.159 ± 0.082	0.435AsnCys: 0.435 ± 0.027	2.787AsnAsp: 2.787 ± 0.063	3.293AsnGlu: 3.293 ± 0.073	3.526AsnPhe: 3.526 ± 0.081	3.992AsnGly: 3.992 ± 0.081	1.034AsnHis: 1.034 ± 0.034	4.665AsnIle: 4.665 ± 0.077	3.492AsnLys: 3.492 ± 0.07	5.82AsnLeu: 5.82 ± 0.092	1.266AsnMet: 1.266 ± 0.037	3.318AsnAsn: 3.318 ± 0.088	2.963AsnPro: 2.963 ± 0.06	2.038AsnGln: 2.038 ± 0.059	2.269AsnArg: 2.269 ± 0.056	3.861AsnSer: 3.861 ± 0.072	3.229AsnThr: 3.229 ± 0.076	3.523AsnVal: 3.523 ± 0.08	0.746AsnTrp: 0.746 ± 0.033	2.723AsnTyr: 2.723 ± 0.058	0.0AsnXaa: 0.0 ± 0.0
Pro	2.739ProAla: 2.739 ± 0.06	0.238ProCys: 0.238 ± 0.018	2.117ProAsp: 2.117 ± 0.056	3.094ProGlu: 3.094 ± 0.068	1.896ProPhe: 1.896 ± 0.047	2.275ProGly: 2.275 ± 0.062	0.661ProHis: 0.661 ± 0.023	2.024ProIle: 2.024 ± 0.047	2.536ProLys: 2.536 ± 0.064	2.993ProLeu: 2.993 ± 0.06	0.83ProMet: 0.83 ± 0.029	2.108ProAsn: 2.108 ± 0.045	0.85ProPro: 0.85 ± 0.038	1.435ProGln: 1.435 ± 0.042	1.15ProArg: 1.15 ± 0.039	2.133ProSer: 2.133 ± 0.05	2.026ProThr: 2.026 ± 0.06	2.894ProVal: 2.894 ± 0.06	0.339ProTrp: 0.339 ± 0.024	1.374ProTyr: 1.374 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	2.08GlnAla: 2.08 ± 0.056	0.186GlnCys: 0.186 ± 0.016	1.709GlnAsp: 1.709 ± 0.051	1.98GlnGlu: 1.98 ± 0.055	1.667GlnPhe: 1.667 ± 0.049	1.762GlnGly: 1.762 ± 0.048	0.703GlnHis: 0.703 ± 0.03	2.818GlnIle: 2.818 ± 0.063	3.27GlnLys: 3.27 ± 0.074	3.293GlnLeu: 3.293 ± 0.061	0.99GlnMet: 0.99 ± 0.031	2.801GlnAsn: 2.801 ± 0.062	1.225GlnPro: 1.225 ± 0.043	1.643GlnGln: 1.643 ± 0.052	1.404GlnArg: 1.404 ± 0.045	2.0GlnSer: 2.0 ± 0.055	2.177GlnThr: 2.177 ± 0.05	1.889GlnVal: 1.889 ± 0.05	0.357GlnTrp: 0.357 ± 0.023	1.339GlnTyr: 1.339 ± 0.041	0.0GlnXaa: 0.0 ± 0.0
Arg	2.184ArgAla: 2.184 ± 0.056	0.22ArgCys: 0.22 ± 0.015	1.936ArgAsp: 1.936 ± 0.051	2.592ArgGlu: 2.592 ± 0.059	2.142ArgPhe: 2.142 ± 0.057	2.034ArgGly: 2.034 ± 0.061	0.72ArgHis: 0.72 ± 0.03	3.105ArgIle: 3.105 ± 0.057	3.238ArgLys: 3.238 ± 0.062	3.259ArgLeu: 3.259 ± 0.056	1.104ArgMet: 1.104 ± 0.034	2.658ArgAsn: 2.658 ± 0.063	1.254ArgPro: 1.254 ± 0.041	1.403ArgGln: 1.403 ± 0.038	1.576ArgArg: 1.576 ± 0.042	2.085ArgSer: 2.085 ± 0.052	2.162ArgThr: 2.162 ± 0.057	2.089ArgVal: 2.089 ± 0.049	0.406ArgTrp: 0.406 ± 0.026	1.479ArgTyr: 1.479 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	5.111SerAla: 5.111 ± 0.083	0.613SerCys: 0.613 ± 0.028	3.569SerAsp: 3.569 ± 0.062	4.209SerGlu: 4.209 ± 0.078	3.616SerPhe: 3.616 ± 0.074	5.267SerGly: 5.267 ± 0.104	1.071SerHis: 1.071 ± 0.034	4.037SerIle: 4.037 ± 0.07	4.186SerLys: 4.186 ± 0.076	5.714SerLeu: 5.714 ± 0.095	1.393SerMet: 1.393 ± 0.041	3.184SerAsn: 3.184 ± 0.073	2.287SerPro: 2.287 ± 0.05	2.235SerGln: 2.235 ± 0.053	2.341SerArg: 2.341 ± 0.052	4.091SerSer: 4.091 ± 0.085	3.553SerThr: 3.553 ± 0.085	4.608SerVal: 4.608 ± 0.071	0.72SerTrp: 0.72 ± 0.033	2.554SerTyr: 2.554 ± 0.059	0.0SerXaa: 0.0 ± 0.0
Thr	4.526ThrAla: 4.526 ± 0.1	0.341ThrCys: 0.341 ± 0.022	3.535ThrAsp: 3.535 ± 0.061	3.786ThrGlu: 3.786 ± 0.072	2.949ThrPhe: 2.949 ± 0.062	4.363ThrGly: 4.363 ± 0.096	0.939ThrHis: 0.939 ± 0.033	3.635ThrIle: 3.635 ± 0.069	3.655ThrLys: 3.655 ± 0.063	5.248ThrLeu: 5.248 ± 0.074	1.157ThrMet: 1.157 ± 0.04	2.895ThrAsn: 2.895 ± 0.067	2.382ThrPro: 2.382 ± 0.063	2.072ThrGln: 2.072 ± 0.047	1.829ThrArg: 1.829 ± 0.05	3.488ThrSer: 3.488 ± 0.076	3.253ThrThr: 3.253 ± 0.091	4.351ThrVal: 4.351 ± 0.091	0.578ThrTrp: 0.578 ± 0.031	2.192ThrTyr: 2.192 ± 0.054	0.0ThrXaa: 0.0 ± 0.0
Val	4.592ValAla: 4.592 ± 0.08	0.524ValCys: 0.524 ± 0.026	3.283ValAsp: 3.283 ± 0.069	3.902ValGlu: 3.902 ± 0.074	3.492ValPhe: 3.492 ± 0.07	4.043ValGly: 4.043 ± 0.076	0.967ValHis: 0.967 ± 0.036	4.64ValIle: 4.64 ± 0.08	4.491ValLys: 4.491 ± 0.07	5.957ValLeu: 5.957 ± 0.086	1.58ValMet: 1.58 ± 0.042	3.611ValAsn: 3.611 ± 0.079	2.381ValPro: 2.381 ± 0.049	2.094ValGln: 2.094 ± 0.052	2.258ValArg: 2.258 ± 0.052	4.579ValSer: 4.579 ± 0.08	3.604ValThr: 3.604 ± 0.082	4.055ValVal: 4.055 ± 0.084	0.652ValTrp: 0.652 ± 0.026	2.48ValTyr: 2.48 ± 0.062	0.0ValXaa: 0.0 ± 0.0
Trp	0.664TrpAla: 0.664 ± 0.029	0.073TrpCys: 0.073 ± 0.009	0.626TrpAsp: 0.626 ± 0.031	0.628TrpGlu: 0.628 ± 0.03	0.587TrpPhe: 0.587 ± 0.03	0.67TrpGly: 0.67 ± 0.032	0.202TrpHis: 0.202 ± 0.018	0.718TrpIle: 0.718 ± 0.035	0.828TrpLys: 0.828 ± 0.031	0.959TrpLeu: 0.959 ± 0.037	0.328TrpMet: 0.328 ± 0.021	0.724TrpAsn: 0.724 ± 0.03	0.192TrpPro: 0.192 ± 0.015	0.452TrpGln: 0.452 ± 0.025	0.357TrpArg: 0.357 ± 0.019	0.579TrpSer: 0.579 ± 0.03	0.592TrpThr: 0.592 ± 0.03	0.59TrpVal: 0.59 ± 0.026	0.145TrpTrp: 0.145 ± 0.014	0.395TrpTyr: 0.395 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.471TyrAla: 2.471 ± 0.064	0.341TyrCys: 0.341 ± 0.023	2.282TyrAsp: 2.282 ± 0.053	2.19TyrGlu: 2.19 ± 0.048	2.541TyrPhe: 2.541 ± 0.069	2.58TyrGly: 2.58 ± 0.059	0.78TyrHis: 0.78 ± 0.029	2.486TyrIle: 2.486 ± 0.054	2.508TyrLys: 2.508 ± 0.06	3.729TyrLeu: 3.729 ± 0.077	0.769TyrMet: 0.769 ± 0.03	2.222TyrAsn: 2.222 ± 0.068	1.547TyrPro: 1.547 ± 0.045	1.523TyrGln: 1.523 ± 0.044	1.862TyrArg: 1.862 ± 0.051	2.76TyrSer: 2.76 ± 0.062	2.198TyrThr: 2.198 ± 0.061	2.068TyrVal: 2.068 ± 0.048	0.456TyrTrp: 0.456 ± 0.025	1.891TyrTyr: 1.891 ± 0.058	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2533 proteins (832499 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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