Halomonas sp. GFAJ-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas; unclassified Halomonas

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3264 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H0J2E0|H0J2E0_9GAMM AIG2 family protein OS=Halomonas sp. GFAJ-1 OX=1118153 GN=MOY_09002 PE=4 SV=1
MM1 pKa = 7.23EE2 pKa = 5.27RR3 pKa = 11.84ALATGEE9 pKa = 4.21TLADD13 pKa = 3.98DD14 pKa = 4.71LGVLIDD20 pKa = 4.17QDD22 pKa = 3.56AHH24 pKa = 6.01YY25 pKa = 10.01LPSAALTTCC34 pKa = 4.11

Molecular weight: 3.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H0J660|H0J660_9GAMM DNA-binding transcriptional regulator IlvY OS=Halomonas sp. GFAJ-1 OX=1118153 GN=MOY_15668 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.42RR12 pKa = 11.84KK13 pKa = 9.04RR14 pKa = 11.84AHH16 pKa = 6.12GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.21NGRR28 pKa = 11.84AVISRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.97GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3264

0

3264

1075868

23

8527

329.6

36.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.795 ± 0.052

0.91 ± 0.014

5.447 ± 0.047

6.311 ± 0.044

3.589 ± 0.026

7.64 ± 0.049

2.484 ± 0.027

5.057 ± 0.037

3.186 ± 0.036

11.327 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.578 ± 0.024

3.082 ± 0.029

4.708 ± 0.04

4.445 ± 0.033

6.296 ± 0.04

5.887 ± 0.036

5.239 ± 0.027

7.137 ± 0.039

1.445 ± 0.022

2.434 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski