Amino acid dipepetide frequency for Halomonas sp. GFAJ-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.481AlaAla: 11.481 ± 0.134	1.134AlaCys: 1.134 ± 0.034	5.486AlaAsp: 5.486 ± 0.095	6.724AlaGlu: 6.724 ± 0.087	4.051AlaPhe: 4.051 ± 0.072	7.925AlaGly: 7.925 ± 0.118	2.448AlaHis: 2.448 ± 0.054	5.569AlaIle: 5.569 ± 0.081	3.497AlaLys: 3.497 ± 0.062	14.059AlaLeu: 14.059 ± 0.173	3.241AlaMet: 3.241 ± 0.052	3.353AlaAsn: 3.353 ± 0.064	4.565AlaPro: 4.565 ± 0.079	4.727AlaGln: 4.727 ± 0.074	6.459AlaArg: 6.459 ± 0.087	6.499AlaSer: 6.499 ± 0.097	5.368AlaThr: 5.368 ± 0.078	7.136AlaVal: 7.136 ± 0.093	1.689AlaTrp: 1.689 ± 0.04	2.537AlaTyr: 2.537 ± 0.051	0.002AlaXaa: 0.002 ± 0.001
Cys	0.881CysAla: 0.881 ± 0.033	0.12CysCys: 0.12 ± 0.011	0.554CysAsp: 0.554 ± 0.024	0.539CysGlu: 0.539 ± 0.024	0.336CysPhe: 0.336 ± 0.017	0.829CysGly: 0.829 ± 0.031	0.338CysHis: 0.338 ± 0.016	0.397CysIle: 0.397 ± 0.019	0.241CysLys: 0.241 ± 0.016	0.969CysLeu: 0.969 ± 0.033	0.205CysMet: 0.205 ± 0.014	0.22CysAsn: 0.22 ± 0.015	0.48CysPro: 0.48 ± 0.025	0.442CysGln: 0.442 ± 0.023	0.571CysArg: 0.571 ± 0.024	0.481CysSer: 0.481 ± 0.023	0.409CysThr: 0.409 ± 0.019	0.687CysVal: 0.687 ± 0.03	0.129CysTrp: 0.129 ± 0.012	0.273CysTyr: 0.273 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	6.348AspAla: 6.348 ± 0.1	0.37AspCys: 0.37 ± 0.02	3.476AspAsp: 3.476 ± 0.091	3.897AspGlu: 3.897 ± 0.066	2.039AspPhe: 2.039 ± 0.043	4.037AspGly: 4.037 ± 0.091	1.338AspHis: 1.338 ± 0.043	3.331AspIle: 3.331 ± 0.074	1.678AspLys: 1.678 ± 0.038	4.815AspLeu: 4.815 ± 0.07	1.312AspMet: 1.312 ± 0.037	1.825AspAsn: 1.825 ± 0.052	2.564AspPro: 2.564 ± 0.051	2.087AspGln: 2.087 ± 0.051	2.886AspArg: 2.886 ± 0.058	2.813AspSer: 2.813 ± 0.057	3.025AspThr: 3.025 ± 0.066	4.246AspVal: 4.246 ± 0.082	0.777AspTrp: 0.777 ± 0.027	1.607AspTyr: 1.607 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	7.365GluAla: 7.365 ± 0.102	0.444GluCys: 0.444 ± 0.021	2.824GluAsp: 2.824 ± 0.061	4.173GluGlu: 4.173 ± 0.078	1.699GluPhe: 1.699 ± 0.046	4.573GluGly: 4.573 ± 0.069	1.837GluHis: 1.837 ± 0.043	2.942GluIle: 2.942 ± 0.057	2.315GluLys: 2.315 ± 0.057	6.693GluLeu: 6.693 ± 0.096	1.615GluMet: 1.615 ± 0.042	1.8GluAsn: 1.8 ± 0.046	2.541GluPro: 2.541 ± 0.069	3.918GluGln: 3.918 ± 0.08	5.317GluArg: 5.317 ± 0.085	3.368GluSer: 3.368 ± 0.059	3.191GluThr: 3.191 ± 0.055	4.306GluVal: 4.306 ± 0.073	0.918GluTrp: 0.918 ± 0.032	1.267GluTyr: 1.267 ± 0.033	0.002GluXaa: 0.002 ± 0.001
Phe	3.599PheAla: 3.599 ± 0.068	0.428PheCys: 0.428 ± 0.021	2.406PheAsp: 2.406 ± 0.055	2.255PheGlu: 2.255 ± 0.052	1.396PhePhe: 1.396 ± 0.041	3.137PheGly: 3.137 ± 0.063	0.818PheHis: 0.818 ± 0.029	2.066PheIle: 2.066 ± 0.046	1.19PheLys: 1.19 ± 0.039	3.128PheLeu: 3.128 ± 0.054	0.982PheMet: 0.982 ± 0.032	1.325PheAsn: 1.325 ± 0.032	1.397PhePro: 1.397 ± 0.034	1.3PheGln: 1.3 ± 0.033	1.78PheArg: 1.78 ± 0.044	2.604PheSer: 2.604 ± 0.054	2.102PheThr: 2.102 ± 0.045	2.392PheVal: 2.392 ± 0.053	0.526PheTrp: 0.526 ± 0.024	1.052PheTyr: 1.052 ± 0.036	0.002PheXaa: 0.002 ± 0.001
Gly	6.804GlyAla: 6.804 ± 0.114	0.887GlyCys: 0.887 ± 0.031	4.228GlyAsp: 4.228 ± 0.104	5.108GlyGlu: 5.108 ± 0.081	3.225GlyPhe: 3.225 ± 0.058	5.971GlyGly: 5.971 ± 0.121	1.918GlyHis: 1.918 ± 0.054	4.413GlyIle: 4.413 ± 0.072	2.932GlyLys: 2.932 ± 0.057	8.436GlyLeu: 8.436 ± 0.119	2.274GlyMet: 2.274 ± 0.05	2.329GlyAsn: 2.329 ± 0.063	2.418GlyPro: 2.418 ± 0.061	3.18GlyGln: 3.18 ± 0.061	4.481GlyArg: 4.481 ± 0.071	4.431GlySer: 4.431 ± 0.107	3.63GlyThr: 3.63 ± 0.06	6.113GlyVal: 6.113 ± 0.094	1.267GlyTrp: 1.267 ± 0.037	2.35GlyTyr: 2.35 ± 0.049	0.001GlyXaa: 0.001 ± 0.001
His	2.518HisAla: 2.518 ± 0.054	0.29HisCys: 0.29 ± 0.019	1.365HisAsp: 1.365 ± 0.043	1.236HisGlu: 1.236 ± 0.036	1.077HisPhe: 1.077 ± 0.035	2.074HisGly: 2.074 ± 0.054	0.867HisHis: 0.867 ± 0.035	1.14HisIle: 1.14 ± 0.031	0.658HisLys: 0.658 ± 0.027	2.654HisLeu: 2.654 ± 0.056	0.498HisMet: 0.498 ± 0.023	0.676HisAsn: 0.676 ± 0.023	1.483HisPro: 1.483 ± 0.042	1.27HisGln: 1.27 ± 0.044	1.599HisArg: 1.599 ± 0.041	1.393HisSer: 1.393 ± 0.039	1.213HisThr: 1.213 ± 0.038	1.522HisVal: 1.522 ± 0.04	0.454HisTrp: 0.454 ± 0.022	0.852HisTyr: 0.852 ± 0.031	0.001HisXaa: 0.001 ± 0.001
Ile	6.202IleAla: 6.202 ± 0.078	0.428IleCys: 0.428 ± 0.022	3.526IleAsp: 3.526 ± 0.072	3.826IleGlu: 3.826 ± 0.064	1.589IlePhe: 1.589 ± 0.047	4.294IleGly: 4.294 ± 0.074	1.066IleHis: 1.066 ± 0.035	2.51IleIle: 2.51 ± 0.059	1.707IleLys: 1.707 ± 0.045	3.948IleLeu: 3.948 ± 0.073	1.104IleMet: 1.104 ± 0.035	2.099IleAsn: 2.099 ± 0.062	2.221IlePro: 2.221 ± 0.05	1.652IleGln: 1.652 ± 0.044	2.784IleArg: 2.784 ± 0.055	3.297IleSer: 3.297 ± 0.063	3.165IleThr: 3.165 ± 0.07	3.451IleVal: 3.451 ± 0.064	0.513IleTrp: 0.513 ± 0.021	1.19IleTyr: 1.19 ± 0.035	0.001IleXaa: 0.001 ± 0.001
Lys	3.724LysAla: 3.724 ± 0.071	0.194LysCys: 0.194 ± 0.015	1.471LysAsp: 1.471 ± 0.036	1.989LysGlu: 1.989 ± 0.05	0.669LysPhe: 0.669 ± 0.028	2.444LysGly: 2.444 ± 0.049	0.821LysHis: 0.821 ± 0.028	1.411LysIle: 1.411 ± 0.042	1.354LysLys: 1.354 ± 0.039	3.333LysLeu: 3.333 ± 0.058	0.745LysMet: 0.745 ± 0.029	0.852LysAsn: 0.852 ± 0.031	1.783LysPro: 1.783 ± 0.05	1.64LysGln: 1.64 ± 0.048	2.751LysArg: 2.751 ± 0.052	1.702LysSer: 1.702 ± 0.048	1.73LysThr: 1.73 ± 0.046	2.375LysVal: 2.375 ± 0.054	0.319LysTrp: 0.319 ± 0.019	0.557LysTyr: 0.557 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	13.523LeuAla: 13.523 ± 0.157	1.088LeuCys: 1.088 ± 0.03	6.081LeuAsp: 6.081 ± 0.075	7.217LeuGlu: 7.217 ± 0.09	3.973LeuPhe: 3.973 ± 0.08	8.539LeuGly: 8.539 ± 0.109	2.419LeuHis: 2.419 ± 0.051	5.708LeuIle: 5.708 ± 0.089	3.852LeuLys: 3.852 ± 0.073	12.469LeuLeu: 12.469 ± 0.187	2.956LeuMet: 2.956 ± 0.058	3.701LeuAsn: 3.701 ± 0.064	6.191LeuPro: 6.191 ± 0.091	3.644LeuGln: 3.644 ± 0.064	6.437LeuArg: 6.437 ± 0.086	7.477LeuSer: 7.477 ± 0.096	6.464LeuThr: 6.464 ± 0.086	7.701LeuVal: 7.701 ± 0.101	1.429LeuTrp: 1.429 ± 0.045	2.401LeuTyr: 2.401 ± 0.05	0.003LeuXaa: 0.003 ± 0.002
Met	3.183MetAla: 3.183 ± 0.064	0.169MetCys: 0.169 ± 0.013	1.153MetAsp: 1.153 ± 0.032	1.233MetGlu: 1.233 ± 0.038	0.717MetPhe: 0.717 ± 0.026	2.011MetGly: 2.011 ± 0.045	0.541MetHis: 0.541 ± 0.023	1.243MetIle: 1.243 ± 0.039	0.804MetLys: 0.804 ± 0.03	3.028MetLeu: 3.028 ± 0.062	0.693MetMet: 0.693 ± 0.025	0.799MetAsn: 0.799 ± 0.025	1.479MetPro: 1.479 ± 0.044	1.182MetGln: 1.182 ± 0.034	1.614MetArg: 1.614 ± 0.041	1.874MetSer: 1.874 ± 0.04	1.553MetThr: 1.553 ± 0.042	1.877MetVal: 1.877 ± 0.043	0.227MetTrp: 0.227 ± 0.015	0.396MetTyr: 0.396 ± 0.02	0.001MetXaa: 0.001 ± 0.001
Asn	3.711AsnAla: 3.711 ± 0.07	0.264AsnCys: 0.264 ± 0.018	1.866AsnAsp: 1.866 ± 0.057	1.749AsnGlu: 1.749 ± 0.047	0.995AsnPhe: 0.995 ± 0.033	2.37AsnGly: 2.37 ± 0.054	0.739AsnHis: 0.739 ± 0.027	1.623AsnIle: 1.623 ± 0.048	0.849AsnLys: 0.849 ± 0.032	2.896AsnLeu: 2.896 ± 0.053	0.677AsnMet: 0.677 ± 0.026	1.03AsnAsn: 1.03 ± 0.039	1.788AsnPro: 1.788 ± 0.043	1.43AsnGln: 1.43 ± 0.039	1.979AsnArg: 1.979 ± 0.045	1.614AsnSer: 1.614 ± 0.045	1.682AsnThr: 1.682 ± 0.05	2.326AsnVal: 2.326 ± 0.062	0.45AsnTrp: 0.45 ± 0.021	0.782AsnTyr: 0.782 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	4.646ProAla: 4.646 ± 0.081	0.348ProCys: 0.348 ± 0.016	2.856ProAsp: 2.856 ± 0.054	3.645ProGlu: 3.645 ± 0.075	1.738ProPhe: 1.738 ± 0.042	3.607ProGly: 3.607 ± 0.07	1.132ProHis: 1.132 ± 0.036	2.223ProIle: 2.223 ± 0.049	1.481ProLys: 1.481 ± 0.045	5.616ProLeu: 5.616 ± 0.089	1.175ProMet: 1.175 ± 0.034	1.549ProAsn: 1.549 ± 0.042	2.052ProPro: 2.052 ± 0.049	1.841ProGln: 1.841 ± 0.045	2.432ProArg: 2.432 ± 0.058	2.884ProSer: 2.884 ± 0.054	2.434ProThr: 2.434 ± 0.048	3.553ProVal: 3.553 ± 0.06	0.8ProTrp: 0.8 ± 0.031	1.065ProTyr: 1.065 ± 0.029	0.001ProXaa: 0.001 ± 0.001
Gln	5.677GlnAla: 5.677 ± 0.095	0.365GlnCys: 0.365 ± 0.018	1.775GlnAsp: 1.775 ± 0.045	2.464GlnGlu: 2.464 ± 0.055	1.244GlnPhe: 1.244 ± 0.034	3.389GlnGly: 3.389 ± 0.052	1.313GlnHis: 1.313 ± 0.039	1.713GlnIle: 1.713 ± 0.041	1.203GlnLys: 1.203 ± 0.038	5.426GlnLeu: 5.426 ± 0.083	1.032GlnMet: 1.032 ± 0.036	1.041GlnAsn: 1.041 ± 0.035	2.168GlnPro: 2.168 ± 0.055	3.02GlnGln: 3.02 ± 0.075	3.845GlnArg: 3.845 ± 0.078	2.168GlnSer: 2.168 ± 0.048	1.992GlnThr: 1.992 ± 0.044	3.072GlnVal: 3.072 ± 0.059	0.765GlnTrp: 0.765 ± 0.026	0.774GlnTyr: 0.774 ± 0.028	0.001GlnXaa: 0.001 ± 0.001
Arg	5.363ArgAla: 5.363 ± 0.082	0.6ArgCys: 0.6 ± 0.022	3.633ArgAsp: 3.633 ± 0.062	4.45ArgGlu: 4.45 ± 0.082	2.849ArgPhe: 2.849 ± 0.063	3.816ArgGly: 3.816 ± 0.06	1.978ArgHis: 1.978 ± 0.049	3.253ArgIle: 3.253 ± 0.065	1.902ArgLys: 1.902 ± 0.052	8.253ArgLeu: 8.253 ± 0.111	1.627ArgMet: 1.627 ± 0.036	1.799ArgAsn: 1.799 ± 0.039	2.399ArgPro: 2.399 ± 0.05	3.422ArgGln: 3.422 ± 0.063	4.276ArgArg: 4.276 ± 0.082	3.147ArgSer: 3.147 ± 0.047	2.648ArgThr: 2.648 ± 0.051	4.265ArgVal: 4.265 ± 0.064	1.118ArgTrp: 1.118 ± 0.041	2.165ArgTyr: 2.165 ± 0.047	0.001ArgXaa: 0.001 ± 0.001
Ser	6.158SerAla: 6.158 ± 0.089	0.448SerCys: 0.448 ± 0.021	3.289SerAsp: 3.289 ± 0.077	3.431SerGlu: 3.431 ± 0.071	2.179SerPhe: 2.179 ± 0.048	5.182SerGly: 5.182 ± 0.106	1.471SerHis: 1.471 ± 0.04	2.824SerIle: 2.824 ± 0.062	1.603SerLys: 1.603 ± 0.039	6.934SerLeu: 6.934 ± 0.101	1.497SerMet: 1.497 ± 0.034	1.682SerAsn: 1.682 ± 0.046	2.794SerPro: 2.794 ± 0.053	2.748SerGln: 2.748 ± 0.052	3.645SerArg: 3.645 ± 0.056	3.523SerSer: 3.523 ± 0.082	3.056SerThr: 3.056 ± 0.059	4.263SerVal: 4.263 ± 0.085	0.798SerTrp: 0.798 ± 0.029	1.345SerTyr: 1.345 ± 0.043	0.001SerXaa: 0.001 ± 0.001
Thr	5.354ThrAla: 5.354 ± 0.072	0.43ThrCys: 0.43 ± 0.021	2.567ThrAsp: 2.567 ± 0.062	2.486ThrGlu: 2.486 ± 0.05	1.982ThrPhe: 1.982 ± 0.045	4.037ThrGly: 4.037 ± 0.062	1.341ThrHis: 1.341 ± 0.034	2.395ThrIle: 2.395 ± 0.054	1.229ThrLys: 1.229 ± 0.042	7.781ThrLeu: 7.781 ± 0.102	1.09ThrMet: 1.09 ± 0.031	1.366ThrAsn: 1.366 ± 0.041	3.643ThrPro: 3.643 ± 0.059	2.357ThrGln: 2.357 ± 0.05	3.184ThrArg: 3.184 ± 0.06	2.865ThrSer: 2.865 ± 0.057	2.83ThrThr: 2.83 ± 0.056	3.586ThrVal: 3.586 ± 0.077	0.721ThrTrp: 0.721 ± 0.03	1.147ThrTyr: 1.147 ± 0.033	0.001ThrXaa: 0.001 ± 0.001
Val	7.819ValAla: 7.819 ± 0.096	0.635ValCys: 0.635 ± 0.024	4.057ValAsp: 4.057 ± 0.08	4.731ValGlu: 4.731 ± 0.072	2.566ValPhe: 2.566 ± 0.055	5.335ValGly: 5.335 ± 0.083	1.372ValHis: 1.372 ± 0.035	4.122ValIle: 4.122 ± 0.072	2.333ValLys: 2.333 ± 0.048	7.573ValLeu: 7.573 ± 0.111	2.166ValMet: 2.166 ± 0.05	2.357ValAsn: 2.357 ± 0.054	3.113ValPro: 3.113 ± 0.054	2.175ValGln: 2.175 ± 0.049	3.918ValArg: 3.918 ± 0.057	4.661ValSer: 4.661 ± 0.094	4.131ValThr: 4.131 ± 0.068	5.924ValVal: 5.924 ± 0.083	0.859ValTrp: 0.859 ± 0.027	1.52ValTyr: 1.52 ± 0.038	0.002ValXaa: 0.002 ± 0.001
Trp	1.113TrpAla: 1.113 ± 0.031	0.176TrpCys: 0.176 ± 0.013	0.536TrpAsp: 0.536 ± 0.022	0.711TrpGlu: 0.711 ± 0.029	0.547TrpPhe: 0.547 ± 0.023	0.879TrpGly: 0.879 ± 0.03	0.415TrpHis: 0.415 ± 0.02	0.663TrpIle: 0.663 ± 0.029	0.415TrpLys: 0.415 ± 0.022	2.454TrpLeu: 2.454 ± 0.068	0.396TrpMet: 0.396 ± 0.021	0.358TrpAsn: 0.358 ± 0.017	0.709TrpPro: 0.709 ± 0.029	1.113TrpGln: 1.113 ± 0.036	1.071TrpArg: 1.071 ± 0.035	0.71TrpSer: 0.71 ± 0.024	0.522TrpThr: 0.522 ± 0.026	1.036TrpVal: 1.036 ± 0.032	0.292TrpTrp: 0.292 ± 0.018	0.34TrpTyr: 0.34 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.477TyrAla: 2.477 ± 0.046	0.283TyrCys: 0.283 ± 0.017	1.32TyrAsp: 1.32 ± 0.031	1.244TyrGlu: 1.244 ± 0.034	1.013TyrPhe: 1.013 ± 0.034	1.944TyrGly: 1.944 ± 0.044	0.666TyrHis: 0.666 ± 0.025	1.024TyrIle: 1.024 ± 0.033	0.623TyrLys: 0.623 ± 0.026	2.803TyrLeu: 2.803 ± 0.054	0.488TyrMet: 0.488 ± 0.022	0.658TyrAsn: 0.658 ± 0.027	1.293TyrPro: 1.293 ± 0.034	1.301TyrGln: 1.301 ± 0.037	1.931TyrArg: 1.931 ± 0.045	1.362TyrSer: 1.362 ± 0.039	1.247TyrThr: 1.247 ± 0.033	1.536TyrVal: 1.536 ± 0.037	0.403TyrTrp: 0.403 ± 0.02	0.721TyrTyr: 0.721 ± 0.03	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.002XaaAla: 0.002 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.002XaaPhe: 0.002 ± 0.002	0.002XaaGly: 0.002 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.002XaaLeu: 0.002 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.006XaaArg: 0.006 ± 0.003	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.002XaaVal: 0.002 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.01XaaXaa: 0.01 ± 0.01

Statistics based on 3264 proteins (1075868 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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