Amino acid dipepetide frequency for Asticcacaulis biprosthecum C19

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.02AlaAla: 15.02 ± 0.155	1.088AlaCys: 1.088 ± 0.032	6.946AlaAsp: 6.946 ± 0.085	6.279AlaGlu: 6.279 ± 0.079	4.358AlaPhe: 4.358 ± 0.066	10.56AlaGly: 10.56 ± 0.155	2.098AlaHis: 2.098 ± 0.044	5.596AlaIle: 5.596 ± 0.068	4.241AlaLys: 4.241 ± 0.065	11.912AlaLeu: 11.912 ± 0.123	3.178AlaMet: 3.178 ± 0.051	3.293AlaAsn: 3.293 ± 0.067	5.293AlaPro: 5.293 ± 0.072	4.464AlaGln: 4.464 ± 0.066	7.291AlaArg: 7.291 ± 0.093	6.034AlaSer: 6.034 ± 0.064	6.257AlaThr: 6.257 ± 0.071	8.371AlaVal: 8.371 ± 0.087	1.704AlaTrp: 1.704 ± 0.042	3.0AlaTyr: 3.0 ± 0.041	0.005AlaXaa: 0.005 ± 0.002
Cys	0.936CysAla: 0.936 ± 0.031	0.107CysCys: 0.107 ± 0.008	0.562CysAsp: 0.562 ± 0.021	0.407CysGlu: 0.407 ± 0.019	0.287CysPhe: 0.287 ± 0.014	0.807CysGly: 0.807 ± 0.027	0.212CysHis: 0.212 ± 0.012	0.345CysIle: 0.345 ± 0.015	0.236CysLys: 0.236 ± 0.013	0.83CysLeu: 0.83 ± 0.027	0.136CysMet: 0.136 ± 0.01	0.23CysAsn: 0.23 ± 0.013	0.412CysPro: 0.412 ± 0.019	0.224CysGln: 0.224 ± 0.012	0.503CysArg: 0.503 ± 0.02	0.42CysSer: 0.42 ± 0.014	0.427CysThr: 0.427 ± 0.019	0.608CysVal: 0.608 ± 0.019	0.103CysTrp: 0.103 ± 0.008	0.205CysTyr: 0.205 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	6.845AspAla: 6.845 ± 0.068	0.464AspCys: 0.464 ± 0.019	4.023AspAsp: 4.023 ± 0.101	3.162AspGlu: 3.162 ± 0.052	2.535AspPhe: 2.535 ± 0.039	6.368AspGly: 6.368 ± 0.143	1.391AspHis: 1.391 ± 0.032	3.534AspIle: 3.534 ± 0.05	2.106AspLys: 2.106 ± 0.039	6.422AspLeu: 6.422 ± 0.077	1.494AspMet: 1.494 ± 0.035	1.902AspAsn: 1.902 ± 0.047	3.288AspPro: 3.288 ± 0.061	1.962AspGln: 1.962 ± 0.04	3.862AspArg: 3.862 ± 0.057	3.053AspSer: 3.053 ± 0.072	3.843AspThr: 3.843 ± 0.155	4.4AspVal: 4.4 ± 0.071	1.009AspTrp: 1.009 ± 0.029	1.96AspTyr: 1.96 ± 0.035	0.001AspXaa: 0.001 ± 0.001
Glu	6.953GluAla: 6.953 ± 0.084	0.25GluCys: 0.25 ± 0.015	2.938GluAsp: 2.938 ± 0.054	2.23GluGlu: 2.23 ± 0.055	1.63GluPhe: 1.63 ± 0.036	4.174GluGly: 4.174 ± 0.07	0.982GluHis: 0.982 ± 0.025	2.908GluIle: 2.908 ± 0.048	2.146GluLys: 2.146 ± 0.046	4.524GluLeu: 4.524 ± 0.064	1.267GluMet: 1.267 ± 0.031	1.597GluAsn: 1.597 ± 0.04	2.297GluPro: 2.297 ± 0.046	1.793GluGln: 1.793 ± 0.044	3.642GluArg: 3.642 ± 0.064	2.431GluSer: 2.431 ± 0.037	3.455GluThr: 3.455 ± 0.051	3.627GluVal: 3.627 ± 0.055	0.631GluTrp: 0.631 ± 0.02	0.99GluTyr: 0.99 ± 0.023	0.001GluXaa: 0.001 ± 0.001
Phe	4.323PheAla: 4.323 ± 0.06	0.359PheCys: 0.359 ± 0.015	3.051PheAsp: 3.051 ± 0.048	2.246PheGlu: 2.246 ± 0.045	1.402PhePhe: 1.402 ± 0.034	3.625PheGly: 3.625 ± 0.05	0.763PheHis: 0.763 ± 0.022	1.717PheIle: 1.717 ± 0.037	1.325PheLys: 1.325 ± 0.03	3.18PheLeu: 3.18 ± 0.055	0.844PheMet: 0.844 ± 0.024	1.35PheAsn: 1.35 ± 0.032	1.451PhePro: 1.451 ± 0.031	1.078PheGln: 1.078 ± 0.028	2.027PheArg: 2.027 ± 0.038	2.388PheSer: 2.388 ± 0.043	2.209PheThr: 2.209 ± 0.042	2.745PheVal: 2.745 ± 0.04	0.561PheTrp: 0.561 ± 0.023	1.054PheTyr: 1.054 ± 0.029	0.002PheXaa: 0.002 ± 0.001
Gly	8.899GlyAla: 8.899 ± 0.131	0.762GlyCys: 0.762 ± 0.026	5.764GlyAsp: 5.764 ± 0.184	4.511GlyGlu: 4.511 ± 0.072	3.788GlyPhe: 3.788 ± 0.058	8.852GlyGly: 8.852 ± 0.406	1.956GlyHis: 1.956 ± 0.037	4.052GlyIle: 4.052 ± 0.059	3.487GlyLys: 3.487 ± 0.055	9.03GlyLeu: 9.03 ± 0.088	2.102GlyMet: 2.102 ± 0.045	3.836GlyAsn: 3.836 ± 0.233	3.191GlyPro: 3.191 ± 0.05	3.253GlyGln: 3.253 ± 0.051	5.26GlyArg: 5.26 ± 0.071	4.637GlySer: 4.637 ± 0.103	4.859GlyThr: 4.859 ± 0.112	6.379GlyVal: 6.379 ± 0.081	1.414GlyTrp: 1.414 ± 0.038	2.664GlyTyr: 2.664 ± 0.052	0.003GlyXaa: 0.003 ± 0.001
His	2.145HisAla: 2.145 ± 0.048	0.199HisCys: 0.199 ± 0.011	1.382HisAsp: 1.382 ± 0.029	0.967HisGlu: 0.967 ± 0.028	0.797HisPhe: 0.797 ± 0.023	1.843HisGly: 1.843 ± 0.04	0.546HisHis: 0.546 ± 0.025	1.051HisIle: 1.051 ± 0.026	0.6HisLys: 0.6 ± 0.023	2.104HisLeu: 2.104 ± 0.04	0.502HisMet: 0.502 ± 0.018	0.582HisAsn: 0.582 ± 0.018	1.15HisPro: 1.15 ± 0.029	0.618HisGln: 0.618 ± 0.022	1.254HisArg: 1.254 ± 0.032	0.948HisSer: 0.948 ± 0.024	0.895HisThr: 0.895 ± 0.03	1.384HisVal: 1.384 ± 0.031	0.335HisTrp: 0.335 ± 0.014	0.621HisTyr: 0.621 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	6.096IleAla: 6.096 ± 0.083	0.468IleCys: 0.468 ± 0.019	3.803IleAsp: 3.803 ± 0.058	3.166IleGlu: 3.166 ± 0.054	1.657IlePhe: 1.657 ± 0.034	4.506IleGly: 4.506 ± 0.065	0.934IleHis: 0.934 ± 0.022	2.168IleIle: 2.168 ± 0.041	1.677IleLys: 1.677 ± 0.038	4.3IleLeu: 4.3 ± 0.064	1.013IleMet: 1.013 ± 0.028	1.76IleAsn: 1.76 ± 0.04	2.052IlePro: 2.052 ± 0.038	1.423IleGln: 1.423 ± 0.033	2.972IleArg: 2.972 ± 0.044	3.032IleSer: 3.032 ± 0.053	2.929IleThr: 2.929 ± 0.053	3.724IleVal: 3.724 ± 0.049	0.642IleTrp: 0.642 ± 0.02	1.305IleTyr: 1.305 ± 0.028	0.002IleXaa: 0.002 ± 0.001
Lys	5.111LysAla: 5.111 ± 0.072	0.174LysCys: 0.174 ± 0.011	2.16LysAsp: 2.16 ± 0.044	1.371LysGlu: 1.371 ± 0.042	1.092LysPhe: 1.092 ± 0.026	3.1LysGly: 3.1 ± 0.053	0.637LysHis: 0.637 ± 0.024	1.856LysIle: 1.856 ± 0.041	1.52LysLys: 1.52 ± 0.036	3.461LysLeu: 3.461 ± 0.05	0.825LysMet: 0.825 ± 0.027	0.998LysAsn: 0.998 ± 0.026	2.431LysPro: 2.431 ± 0.055	1.137LysGln: 1.137 ± 0.032	2.293LysArg: 2.293 ± 0.043	2.18LysSer: 2.18 ± 0.043	2.584LysThr: 2.584 ± 0.049	2.862LysVal: 2.862 ± 0.05	0.463LysTrp: 0.463 ± 0.018	0.779LysTyr: 0.779 ± 0.023	0.001LysXaa: 0.001 ± 0.001
Leu	11.459LeuAla: 11.459 ± 0.102	0.852LeuCys: 0.852 ± 0.029	6.006LeuAsp: 6.006 ± 0.076	4.721LeuGlu: 4.721 ± 0.076	3.467LeuPhe: 3.467 ± 0.06	7.785LeuGly: 7.785 ± 0.081	1.803LeuHis: 1.803 ± 0.041	5.219LeuIle: 5.219 ± 0.068	4.498LeuLys: 4.498 ± 0.067	8.053LeuLeu: 8.053 ± 0.102	2.429LeuMet: 2.429 ± 0.045	3.584LeuAsn: 3.584 ± 0.054	4.918LeuPro: 4.918 ± 0.08	2.736LeuGln: 2.736 ± 0.045	5.789LeuArg: 5.789 ± 0.079	6.832LeuSer: 6.832 ± 0.077	6.699LeuThr: 6.699 ± 0.098	6.411LeuVal: 6.411 ± 0.073	1.284LeuTrp: 1.284 ± 0.037	2.381LeuTyr: 2.381 ± 0.04	0.003LeuXaa: 0.003 ± 0.002
Met	3.182MetAla: 3.182 ± 0.05	0.157MetCys: 0.157 ± 0.011	1.224MetAsp: 1.224 ± 0.032	0.987MetGlu: 0.987 ± 0.026	0.712MetPhe: 0.712 ± 0.026	1.858MetGly: 1.858 ± 0.035	0.367MetHis: 0.367 ± 0.013	1.244MetIle: 1.244 ± 0.031	1.113MetLys: 1.113 ± 0.033	2.012MetLeu: 2.012 ± 0.042	0.613MetMet: 0.613 ± 0.02	0.783MetAsn: 0.783 ± 0.024	1.348MetPro: 1.348 ± 0.033	0.792MetGln: 0.792 ± 0.023	1.444MetArg: 1.444 ± 0.031	1.696MetSer: 1.696 ± 0.032	1.91MetThr: 1.91 ± 0.037	1.593MetVal: 1.593 ± 0.031	0.26MetTrp: 0.26 ± 0.014	0.384MetTyr: 0.384 ± 0.016	0.001MetXaa: 0.001 ± 0.001
Asn	3.582AsnAla: 3.582 ± 0.055	0.235AsnCys: 0.235 ± 0.013	2.535AsnAsp: 2.535 ± 0.157	1.283AsnGlu: 1.283 ± 0.033	1.202AsnPhe: 1.202 ± 0.033	3.302AsnGly: 3.302 ± 0.083	0.633AsnHis: 0.633 ± 0.022	1.665AsnIle: 1.665 ± 0.038	0.841AsnLys: 0.841 ± 0.027	3.373AsnLeu: 3.373 ± 0.057	0.674AsnMet: 0.674 ± 0.021	1.128AsnAsn: 1.128 ± 0.044	1.911AsnPro: 1.911 ± 0.042	0.969AsnGln: 0.969 ± 0.03	1.886AsnArg: 1.886 ± 0.035	1.786AsnSer: 1.786 ± 0.05	1.728AsnThr: 1.728 ± 0.046	2.305AsnVal: 2.305 ± 0.06	0.502AsnTrp: 0.502 ± 0.02	0.9AsnTyr: 0.9 ± 0.029	0.003AsnXaa: 0.003 ± 0.001
Pro	5.778ProAla: 5.778 ± 0.083	0.334ProCys: 0.334 ± 0.014	3.621ProAsp: 3.621 ± 0.057	3.207ProGlu: 3.207 ± 0.054	1.938ProPhe: 1.938 ± 0.036	3.956ProGly: 3.956 ± 0.057	0.933ProHis: 0.933 ± 0.026	2.03ProIle: 2.03 ± 0.042	1.997ProLys: 1.997 ± 0.044	4.179ProLeu: 4.179 ± 0.067	1.084ProMet: 1.084 ± 0.028	1.517ProAsn: 1.517 ± 0.034	2.294ProPro: 2.294 ± 0.061	1.908ProGln: 1.908 ± 0.036	2.299ProArg: 2.299 ± 0.047	2.677ProSer: 2.677 ± 0.051	2.325ProThr: 2.325 ± 0.044	4.226ProVal: 4.226 ± 0.065	0.692ProTrp: 0.692 ± 0.024	1.295ProTyr: 1.295 ± 0.029	0.003ProXaa: 0.003 ± 0.001
Gln	4.568GlnAla: 4.568 ± 0.062	0.208GlnCys: 0.208 ± 0.013	1.719GlnAsp: 1.719 ± 0.034	1.246GlnGlu: 1.246 ± 0.03	1.15GlnPhe: 1.15 ± 0.03	2.772GlnGly: 2.772 ± 0.047	0.672GlnHis: 0.672 ± 0.021	1.853GlnIle: 1.853 ± 0.038	1.322GlnLys: 1.322 ± 0.029	2.766GlnLeu: 2.766 ± 0.042	0.893GlnMet: 0.893 ± 0.025	1.058GlnAsn: 1.058 ± 0.033	1.787GlnPro: 1.787 ± 0.039	1.158GlnGln: 1.158 ± 0.034	2.136GlnArg: 2.136 ± 0.042	2.052GlnSer: 2.052 ± 0.048	2.251GlnThr: 2.251 ± 0.042	2.684GlnVal: 2.684 ± 0.042	0.514GlnTrp: 0.514 ± 0.019	0.817GlnTyr: 0.817 ± 0.024	0.001GlnXaa: 0.001 ± 0.001
Arg	6.327ArgAla: 6.327 ± 0.079	0.449ArgCys: 0.449 ± 0.019	3.979ArgAsp: 3.979 ± 0.063	3.073ArgGlu: 3.073 ± 0.055	2.808ArgPhe: 2.808 ± 0.052	4.214ArgGly: 4.214 ± 0.061	1.495ArgHis: 1.495 ± 0.036	3.355ArgIle: 3.355 ± 0.051	2.289ArgLys: 2.289 ± 0.04	7.094ArgLeu: 7.094 ± 0.09	1.539ArgMet: 1.539 ± 0.039	1.825ArgAsn: 1.825 ± 0.035	3.084ArgPro: 3.084 ± 0.058	2.309ArgGln: 2.309 ± 0.041	4.299ArgArg: 4.299 ± 0.074	3.145ArgSer: 3.145 ± 0.049	3.055ArgThr: 3.055 ± 0.047	4.02ArgVal: 4.02 ± 0.058	0.879ArgTrp: 0.879 ± 0.025	1.787ArgTyr: 1.787 ± 0.037	0.005ArgXaa: 0.005 ± 0.002
Ser	6.32SerAla: 6.32 ± 0.066	0.38SerCys: 0.38 ± 0.016	3.668SerAsp: 3.668 ± 0.063	2.831SerGlu: 2.831 ± 0.048	2.166SerPhe: 2.166 ± 0.04	5.859SerGly: 5.859 ± 0.129	1.21SerHis: 1.21 ± 0.029	2.493SerIle: 2.493 ± 0.051	1.803SerLys: 1.803 ± 0.037	6.012SerLeu: 6.012 ± 0.072	1.246SerMet: 1.246 ± 0.029	1.725SerAsn: 1.725 ± 0.038	2.779SerPro: 2.779 ± 0.052	2.115SerGln: 2.115 ± 0.038	3.319SerArg: 3.319 ± 0.05	3.177SerSer: 3.177 ± 0.06	2.806SerThr: 2.806 ± 0.048	4.157SerVal: 4.157 ± 0.067	0.816SerTrp: 0.816 ± 0.024	1.697SerTyr: 1.697 ± 0.039	0.005SerXaa: 0.005 ± 0.002
Thr	6.816ThrAla: 6.816 ± 0.077	0.484ThrCys: 0.484 ± 0.019	3.197ThrAsp: 3.197 ± 0.057	2.647ThrGlu: 2.647 ± 0.044	2.264ThrPhe: 2.264 ± 0.037	6.355ThrGly: 6.355 ± 0.159	1.058ThrHis: 1.058 ± 0.028	2.762ThrIle: 2.762 ± 0.059	1.742ThrLys: 1.742 ± 0.036	6.389ThrLeu: 6.389 ± 0.112	1.054ThrMet: 1.054 ± 0.029	1.485ThrAsn: 1.485 ± 0.034	3.565ThrPro: 3.565 ± 0.061	1.928ThrGln: 1.928 ± 0.036	3.221ThrArg: 3.221 ± 0.053	3.181ThrSer: 3.181 ± 0.049	3.369ThrThr: 3.369 ± 0.066	4.801ThrVal: 4.801 ± 0.081	0.854ThrTrp: 0.854 ± 0.025	1.899ThrTyr: 1.899 ± 0.048	0.002ThrXaa: 0.002 ± 0.001
Val	8.467ValAla: 8.467 ± 0.084	0.655ValCys: 0.655 ± 0.02	4.241ValAsp: 4.241 ± 0.056	4.229ValGlu: 4.229 ± 0.062	2.894ValPhe: 2.894 ± 0.043	5.122ValGly: 5.122 ± 0.082	1.337ValHis: 1.337 ± 0.031	3.921ValIle: 3.921 ± 0.058	2.715ValLys: 2.715 ± 0.047	7.095ValLeu: 7.095 ± 0.084	1.847ValMet: 1.847 ± 0.037	2.317ValAsn: 2.317 ± 0.047	3.231ValPro: 3.231 ± 0.057	2.159ValGln: 2.159 ± 0.037	4.634ValArg: 4.634 ± 0.067	4.472ValSer: 4.472 ± 0.065	5.111ValThr: 5.111 ± 0.079	5.861ValVal: 5.861 ± 0.077	1.031ValTrp: 1.031 ± 0.025	1.811ValTyr: 1.811 ± 0.032	0.003ValXaa: 0.003 ± 0.001
Trp	1.287TrpAla: 1.287 ± 0.03	0.126TrpCys: 0.126 ± 0.009	0.78TrpAsp: 0.78 ± 0.025	0.554TrpGlu: 0.554 ± 0.022	0.553TrpPhe: 0.553 ± 0.021	1.064TrpGly: 1.064 ± 0.028	0.328TrpHis: 0.328 ± 0.015	0.675TrpIle: 0.675 ± 0.021	0.555TrpLys: 0.555 ± 0.018	1.689TrpLeu: 1.689 ± 0.041	0.396TrpMet: 0.396 ± 0.014	0.504TrpAsn: 0.504 ± 0.02	0.719TrpPro: 0.719 ± 0.026	0.701TrpGln: 0.701 ± 0.02	1.107TrpArg: 1.107 ± 0.031	0.888TrpSer: 0.888 ± 0.025	0.866TrpThr: 0.866 ± 0.031	0.918TrpVal: 0.918 ± 0.026	0.258TrpTrp: 0.258 ± 0.015	0.384TrpTyr: 0.384 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.868TyrAla: 2.868 ± 0.044	0.244TyrCys: 0.244 ± 0.013	2.025TyrAsp: 2.025 ± 0.042	1.352TyrGlu: 1.352 ± 0.033	0.999TyrPhe: 0.999 ± 0.025	2.569TyrGly: 2.569 ± 0.056	0.566TyrHis: 0.566 ± 0.017	1.184TyrIle: 1.184 ± 0.028	0.819TyrLys: 0.819 ± 0.028	2.499TyrLeu: 2.499 ± 0.043	0.542TyrMet: 0.542 ± 0.021	0.969TyrAsn: 0.969 ± 0.034	1.09TyrPro: 1.09 ± 0.031	0.899TyrGln: 0.899 ± 0.026	1.79TyrArg: 1.79 ± 0.037	1.553TyrSer: 1.553 ± 0.038	1.49TyrThr: 1.49 ± 0.035	2.074TyrVal: 2.074 ± 0.043	0.402TyrTrp: 0.402 ± 0.017	0.905TyrTyr: 0.905 ± 0.032	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.005XaaAla: 0.005 ± 0.002	0.001XaaCys: 0.001 ± 0.001	0.002XaaAsp: 0.002 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.007XaaGly: 0.007 ± 0.002	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.003XaaLys: 0.003 ± 0.001	0.004XaaLeu: 0.004 ± 0.002	0.002XaaMet: 0.002 ± 0.001	0.001XaaAsn: 0.001 ± 0.001	0.002XaaPro: 0.002 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.005XaaArg: 0.005 ± 0.002	0.001XaaSer: 0.001 ± 0.001	0.004XaaThr: 0.004 ± 0.002	0.003XaaVal: 0.003 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	1.904XaaXaa: 1.904 ± 0.27

Statistics based on 4712 proteins (1501214 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---