Methylomonas denitrificans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales; Methylococcaceae; Methylomonas

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4463 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126T1Z0|A0A126T1Z0_9GAMM Ferredoxin OS=Methylomonas denitrificans OX=1538553 GN=JT25_006270 PE=4 SV=1
MM1 pKa = 8.01SITTLDD7 pKa = 3.5NASPSLQVLADD18 pKa = 3.84GLKK21 pKa = 10.16AACRR25 pKa = 11.84DD26 pKa = 3.6YY27 pKa = 10.79TQTVQQPDD35 pKa = 3.96SYY37 pKa = 11.88ALTLEE42 pKa = 4.53LGHH45 pKa = 5.55TVRR48 pKa = 11.84YY49 pKa = 8.23LARR52 pKa = 11.84CITLVLCAALGEE64 pKa = 4.57HH65 pKa = 7.38DD66 pKa = 5.0PDD68 pKa = 5.57LDD70 pKa = 3.87EE71 pKa = 6.41LDD73 pKa = 4.13NLSLTDD79 pKa = 5.52LSGEE83 pKa = 4.32DD84 pKa = 4.98DD85 pKa = 4.9DD86 pKa = 4.94EE87 pKa = 5.27QEE89 pKa = 3.92

Molecular weight: 9.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126T628|A0A126T628_9GAMM Molybdenum transport system permease OS=Methylomonas denitrificans OX=1538553 GN=JT25_013660 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.17QPSKK9 pKa = 9.15IKK11 pKa = 10.36RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84RR29 pKa = 11.84VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.12GRR39 pKa = 11.84ASLTVV44 pKa = 3.04

Molecular weight: 5.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4463

0

4463

1456984

29

3519

326.5

36.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.841 ± 0.039

1.036 ± 0.013

5.578 ± 0.027

5.784 ± 0.038

4.036 ± 0.021

7.117 ± 0.047

2.278 ± 0.02

5.999 ± 0.031

4.654 ± 0.032

11.011 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.272 ± 0.018

3.931 ± 0.028

4.37 ± 0.024

4.725 ± 0.027

5.317 ± 0.032

6.116 ± 0.032

5.057 ± 0.034

6.68 ± 0.03

1.336 ± 0.017

2.864 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski