Amino acid dipepetide frequency for Methylomonas denitrificans

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.566AlaAla: 10.566 ± 0.133
1.105AlaCys: 1.105 ± 0.029
5.997AlaAsp: 5.997 ± 0.064
6.982AlaGlu: 6.982 ± 0.091
3.596AlaPhe: 3.596 ± 0.046
7.527AlaGly: 7.527 ± 0.113
1.857AlaHis: 1.857 ± 0.039
6.047AlaIle: 6.047 ± 0.064
4.686AlaLys: 4.686 ± 0.07
10.853AlaLeu: 10.853 ± 0.115
2.645AlaMet: 2.645 ± 0.047
3.798AlaAsn: 3.798 ± 0.057
3.301AlaPro: 3.301 ± 0.061
4.11AlaGln: 4.11 ± 0.059
4.581AlaArg: 4.581 ± 0.061
5.354AlaSer: 5.354 ± 0.07
4.671AlaThr: 4.671 ± 0.075
6.8AlaVal: 6.8 ± 0.084
1.318AlaTrp: 1.318 ± 0.031
2.618AlaTyr: 2.618 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
0.895CysAla: 0.895 ± 0.028
0.164CysCys: 0.164 ± 0.011
0.582CysAsp: 0.582 ± 0.023
0.552CysGlu: 0.552 ± 0.021
0.429CysPhe: 0.429 ± 0.017
0.939CysGly: 0.939 ± 0.029
0.307CysHis: 0.307 ± 0.016
0.5CysIle: 0.5 ± 0.021
0.402CysLys: 0.402 ± 0.015
1.123CysLeu: 1.123 ± 0.033
0.196CysMet: 0.196 ± 0.011
0.344CysAsn: 0.344 ± 0.019
0.483CysPro: 0.483 ± 0.021
0.451CysGln: 0.451 ± 0.017
0.683CysArg: 0.683 ± 0.023
0.692CysSer: 0.692 ± 0.022
0.467CysThr: 0.467 ± 0.022
0.671CysVal: 0.671 ± 0.021
0.14CysTrp: 0.14 ± 0.009
0.343CysTyr: 0.343 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
5.273AspAla: 5.273 ± 0.06
0.642AspCys: 0.642 ± 0.022
3.111AspAsp: 3.111 ± 0.061
3.308AspGlu: 3.308 ± 0.05
2.531AspPhe: 2.531 ± 0.043
4.12AspGly: 4.12 ± 0.072
1.122AspHis: 1.122 ± 0.03
3.649AspIle: 3.649 ± 0.06
2.864AspLys: 2.864 ± 0.053
5.717AspLeu: 5.717 ± 0.065
1.181AspMet: 1.181 ± 0.026
2.194AspAsn: 2.194 ± 0.038
2.334AspPro: 2.334 ± 0.039
2.172AspGln: 2.172 ± 0.042
2.775AspArg: 2.775 ± 0.045
3.361AspSer: 3.361 ± 0.047
2.729AspThr: 2.729 ± 0.059
3.636AspVal: 3.636 ± 0.063
1.038AspTrp: 1.038 ± 0.027
2.024AspTyr: 2.024 ± 0.034
0.0AspXaa: 0.0 ± 0.0
Glu
5.429GluAla: 5.429 ± 0.066
0.48GluCys: 0.48 ± 0.017
2.545GluAsp: 2.545 ± 0.042
2.925GluGlu: 2.925 ± 0.054
2.291GluPhe: 2.291 ± 0.039
2.992GluGly: 2.992 ± 0.053
1.572GluHis: 1.572 ± 0.038
3.936GluIle: 3.936 ± 0.057
3.272GluLys: 3.272 ± 0.057
6.741GluLeu: 6.741 ± 0.083
1.491GluMet: 1.491 ± 0.037
2.343GluAsn: 2.343 ± 0.039
2.173GluPro: 2.173 ± 0.043
3.877GluGln: 3.877 ± 0.061
3.546GluArg: 3.546 ± 0.061
3.205GluSer: 3.205 ± 0.048
3.135GluThr: 3.135 ± 0.05
3.607GluVal: 3.607 ± 0.051
0.723GluTrp: 0.723 ± 0.021
1.555GluTyr: 1.555 ± 0.032
0.0GluXaa: 0.0 ± 0.0
Phe
3.899PheAla: 3.899 ± 0.057
0.523PheCys: 0.523 ± 0.019
2.641PheAsp: 2.641 ± 0.043
2.233PheGlu: 2.233 ± 0.043
1.768PhePhe: 1.768 ± 0.037
3.202PheGly: 3.202 ± 0.052
0.804PheHis: 0.804 ± 0.026
2.325PheIle: 2.325 ± 0.041
1.809PheLys: 1.809 ± 0.037
3.87PheLeu: 3.87 ± 0.055
0.878PheMet: 0.878 ± 0.027
1.769PheAsn: 1.769 ± 0.035
1.565PhePro: 1.565 ± 0.036
1.393PheGln: 1.393 ± 0.034
1.887PheArg: 1.887 ± 0.039
3.077PheSer: 3.077 ± 0.05
2.096PheThr: 2.096 ± 0.035
2.689PheVal: 2.689 ± 0.045
0.595PheTrp: 0.595 ± 0.02
1.337PheTyr: 1.337 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
5.749GlyAla: 5.749 ± 0.087
0.921GlyCys: 0.921 ± 0.027
3.743GlyAsp: 3.743 ± 0.072
4.057GlyGlu: 4.057 ± 0.06
3.471GlyPhe: 3.471 ± 0.051
5.251GlyGly: 5.251 ± 0.116
1.642GlyHis: 1.642 ± 0.035
4.658GlyIle: 4.658 ± 0.061
3.947GlyLys: 3.947 ± 0.053
7.776GlyLeu: 7.776 ± 0.076
1.929GlyMet: 1.929 ± 0.044
2.808GlyAsn: 2.808 ± 0.074
1.934GlyPro: 1.934 ± 0.044
3.099GlyGln: 3.099 ± 0.052
3.636GlyArg: 3.636 ± 0.054
4.479GlySer: 4.479 ± 0.084
3.323GlyThr: 3.323 ± 0.079
5.15GlyVal: 5.15 ± 0.065
1.058GlyTrp: 1.058 ± 0.03
2.536GlyTyr: 2.536 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
2.045HisAla: 2.045 ± 0.037
0.339HisCys: 0.339 ± 0.017
1.272HisAsp: 1.272 ± 0.033
1.196HisGlu: 1.196 ± 0.028
1.065HisPhe: 1.065 ± 0.031
1.742HisGly: 1.742 ± 0.042
0.682HisHis: 0.682 ± 0.029
1.394HisIle: 1.394 ± 0.031
0.961HisLys: 0.961 ± 0.031
2.318HisLeu: 2.318 ± 0.046
0.456HisMet: 0.456 ± 0.019
0.794HisAsn: 0.794 ± 0.025
1.347HisPro: 1.347 ± 0.034
1.05HisGln: 1.05 ± 0.028
1.269HisArg: 1.269 ± 0.033
1.308HisSer: 1.308 ± 0.035
1.0HisThr: 1.0 ± 0.027
1.296HisVal: 1.296 ± 0.033
0.404HisTrp: 0.404 ± 0.016
0.839HisTyr: 0.839 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
6.545IleAla: 6.545 ± 0.068
0.693IleCys: 0.693 ± 0.02
4.205IleAsp: 4.205 ± 0.056
4.028IleGlu: 4.028 ± 0.06
2.03IlePhe: 2.03 ± 0.039
4.741IleGly: 4.741 ± 0.061
1.269IleHis: 1.269 ± 0.031
2.963IleIle: 2.963 ± 0.059
2.96IleLys: 2.96 ± 0.059
5.409IleLeu: 5.409 ± 0.067
1.069IleMet: 1.069 ± 0.029
2.672IleAsn: 2.672 ± 0.048
2.673IlePro: 2.673 ± 0.038
2.117IleGln: 2.117 ± 0.036
3.128IleArg: 3.128 ± 0.047
3.924IleSer: 3.924 ± 0.06
3.032IleThr: 3.032 ± 0.051
4.223IleVal: 4.223 ± 0.057
0.657IleTrp: 0.657 ± 0.024
1.657IleTyr: 1.657 ± 0.035
0.0IleXaa: 0.0 ± 0.0
Lys
4.542LysAla: 4.542 ± 0.069
0.309LysCys: 0.309 ± 0.015
2.334LysAsp: 2.334 ± 0.048
2.194LysGlu: 2.194 ± 0.048
1.393LysPhe: 1.393 ± 0.034
2.644LysGly: 2.644 ± 0.049
1.176LysHis: 1.176 ± 0.029
3.076LysIle: 3.076 ± 0.056
2.35LysLys: 2.35 ± 0.054
5.218LysLeu: 5.218 ± 0.068
1.068LysMet: 1.068 ± 0.028
2.006LysAsn: 2.006 ± 0.043
2.629LysPro: 2.629 ± 0.045
2.936LysGln: 2.936 ± 0.054
2.548LysArg: 2.548 ± 0.047
2.708LysSer: 2.708 ± 0.052
2.876LysThr: 2.876 ± 0.044
2.942LysVal: 2.942 ± 0.056
0.453LysTrp: 0.453 ± 0.018
1.135LysTyr: 1.135 ± 0.03
0.0LysXaa: 0.0 ± 0.0
Leu
11.814LeuAla: 11.814 ± 0.109
1.052LeuCys: 1.052 ± 0.026
6.023LeuAsp: 6.023 ± 0.058
6.159LeuGlu: 6.159 ± 0.078
4.385LeuPhe: 4.385 ± 0.069
7.397LeuGly: 7.397 ± 0.079
2.398LeuHis: 2.398 ± 0.04
6.312LeuIle: 6.312 ± 0.075
5.083LeuLys: 5.083 ± 0.081
12.601LeuLeu: 12.601 ± 0.148
2.36LeuMet: 2.36 ± 0.042
4.371LeuAsn: 4.371 ± 0.059
5.531LeuPro: 5.531 ± 0.079
4.979LeuGln: 4.979 ± 0.064
5.848LeuArg: 5.848 ± 0.068
7.467LeuSer: 7.467 ± 0.077
5.792LeuThr: 5.792 ± 0.076
6.673LeuVal: 6.673 ± 0.081
1.244LeuTrp: 1.244 ± 0.035
2.613LeuTyr: 2.613 ± 0.045
0.0LeuXaa: 0.0 ± 0.0
Met
2.419MetAla: 2.419 ± 0.037
0.132MetCys: 0.132 ± 0.01
1.17MetAsp: 1.17 ± 0.029
1.109MetGlu: 1.109 ± 0.027
0.723MetPhe: 0.723 ± 0.022
1.403MetGly: 1.403 ± 0.033
0.502MetHis: 0.502 ± 0.017
1.229MetIle: 1.229 ± 0.033
1.104MetLys: 1.104 ± 0.027
2.637MetLeu: 2.637 ± 0.043
0.544MetMet: 0.544 ± 0.02
0.998MetAsn: 0.998 ± 0.027
1.368MetPro: 1.368 ± 0.032
1.143MetGln: 1.143 ± 0.03
1.256MetArg: 1.256 ± 0.031
1.531MetSer: 1.531 ± 0.033
1.408MetThr: 1.408 ± 0.027
1.466MetVal: 1.466 ± 0.032
0.161MetTrp: 0.161 ± 0.011
0.415MetTyr: 0.415 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
3.695AsnAla: 3.695 ± 0.057
0.404AsnCys: 0.404 ± 0.018
2.172AsnAsp: 2.172 ± 0.048
1.87AsnGlu: 1.87 ± 0.032
1.423AsnPhe: 1.423 ± 0.029
2.931AsnGly: 2.931 ± 0.059
0.9AsnHis: 0.9 ± 0.024
2.454AsnIle: 2.454 ± 0.046
1.702AsnLys: 1.702 ± 0.035
4.097AsnLeu: 4.097 ± 0.057
0.848AsnMet: 0.848 ± 0.024
1.606AsnAsn: 1.606 ± 0.041
2.369AsnPro: 2.369 ± 0.042
1.988AsnGln: 1.988 ± 0.042
2.256AsnArg: 2.256 ± 0.042
2.409AsnSer: 2.409 ± 0.047
2.071AsnThr: 2.071 ± 0.041
2.327AsnVal: 2.327 ± 0.046
0.586AsnTrp: 0.586 ± 0.023
1.198AsnTyr: 1.198 ± 0.032
0.0AsnXaa: 0.0 ± 0.0
Pro
4.416ProAla: 4.416 ± 0.066
0.377ProCys: 0.377 ± 0.016
2.939ProAsp: 2.939 ± 0.045
3.36ProGlu: 3.36 ± 0.045
1.78ProPhe: 1.78 ± 0.036
3.249ProGly: 3.249 ± 0.054
0.927ProHis: 0.927 ± 0.027
2.446ProIle: 2.446 ± 0.039
1.843ProLys: 1.843 ± 0.04
4.517ProLeu: 4.517 ± 0.056
0.925ProMet: 0.925 ± 0.027
1.658ProAsn: 1.658 ± 0.034
1.762ProPro: 1.762 ± 0.043
1.844ProGln: 1.844 ± 0.039
1.773ProArg: 1.773 ± 0.038
2.469ProSer: 2.469 ± 0.049
2.175ProThr: 2.175 ± 0.043
3.463ProVal: 3.463 ± 0.056
0.596ProTrp: 0.596 ± 0.02
1.187ProTyr: 1.187 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
5.491GlnAla: 5.491 ± 0.068
0.371GlnCys: 0.371 ± 0.017
2.099GlnAsp: 2.099 ± 0.04
2.219GlnGlu: 2.219 ± 0.043
1.736GlnPhe: 1.736 ± 0.041
2.911GlnGly: 2.911 ± 0.046
1.331GlnHis: 1.331 ± 0.033
2.815GlnIle: 2.815 ± 0.044
1.903GlnLys: 1.903 ± 0.042
5.271GlnLeu: 5.271 ± 0.069
1.047GlnMet: 1.047 ± 0.024
1.709GlnAsn: 1.709 ± 0.03
2.111GlnPro: 2.111 ± 0.042
3.264GlnGln: 3.264 ± 0.061
2.92GlnArg: 2.92 ± 0.058
2.636GlnSer: 2.636 ± 0.042
2.638GlnThr: 2.638 ± 0.048
2.896GlnVal: 2.896 ± 0.048
0.619GlnTrp: 0.619 ± 0.021
1.259GlnTyr: 1.259 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
4.17ArgAla: 4.17 ± 0.061
0.556ArgCys: 0.556 ± 0.024
2.872ArgAsp: 2.872 ± 0.042
3.193ArgGlu: 3.193 ± 0.052
2.569ArgPhe: 2.569 ± 0.041
3.128ArgGly: 3.128 ± 0.044
1.43ArgHis: 1.43 ± 0.031
3.513ArgIle: 3.513 ± 0.057
2.42ArgLys: 2.42 ± 0.039
6.28ArgLeu: 6.28 ± 0.077
1.378ArgMet: 1.378 ± 0.03
2.055ArgAsn: 2.055 ± 0.031
2.04ArgPro: 2.04 ± 0.042
3.16ArgGln: 3.16 ± 0.051
3.227ArgArg: 3.227 ± 0.06
2.836ArgSer: 2.836 ± 0.043
2.17ArgThr: 2.17 ± 0.04
3.386ArgVal: 3.386 ± 0.047
0.847ArgTrp: 0.847 ± 0.026
1.938ArgTyr: 1.938 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
6.141SerAla: 6.141 ± 0.071
0.604SerCys: 0.604 ± 0.022
3.182SerAsp: 3.182 ± 0.049
3.424SerGlu: 3.424 ± 0.051
2.605SerPhe: 2.605 ± 0.051
5.295SerGly: 5.295 ± 0.097
1.395SerHis: 1.395 ± 0.032
3.557SerIle: 3.557 ± 0.053
2.636SerLys: 2.636 ± 0.046
6.758SerLeu: 6.758 ± 0.071
1.336SerMet: 1.336 ± 0.029
2.309SerAsn: 2.309 ± 0.04
2.507SerPro: 2.507 ± 0.039
2.57SerGln: 2.57 ± 0.048
3.248SerArg: 3.248 ± 0.048
3.813SerSer: 3.813 ± 0.065
2.952SerThr: 2.952 ± 0.057
4.299SerVal: 4.299 ± 0.053
0.804SerTrp: 0.804 ± 0.024
1.726SerTyr: 1.726 ± 0.032
0.0SerXaa: 0.0 ± 0.0
Thr
5.135ThrAla: 5.135 ± 0.067
0.437ThrCys: 0.437 ± 0.021
2.826ThrAsp: 2.826 ± 0.045
2.927ThrGlu: 2.927 ± 0.042
1.815ThrPhe: 1.815 ± 0.038
4.288ThrGly: 4.288 ± 0.062
1.152ThrHis: 1.152 ± 0.028
2.925ThrIle: 2.925 ± 0.053
1.785ThrLys: 1.785 ± 0.036
6.171ThrLeu: 6.171 ± 0.084
0.939ThrMet: 0.939 ± 0.025
1.707ThrAsn: 1.707 ± 0.047
2.866ThrPro: 2.866 ± 0.048
2.115ThrGln: 2.115 ± 0.044
2.398ThrArg: 2.398 ± 0.042
2.78ThrSer: 2.78 ± 0.058
2.577ThrThr: 2.577 ± 0.054
3.908ThrVal: 3.908 ± 0.061
0.59ThrTrp: 0.59 ± 0.022
1.232ThrTyr: 1.232 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
6.732ValAla: 6.732 ± 0.072
0.728ValCys: 0.728 ± 0.023
3.92ValAsp: 3.92 ± 0.062
4.096ValGlu: 4.096 ± 0.062
2.826ValPhe: 2.826 ± 0.047
4.532ValGly: 4.532 ± 0.063
1.268ValHis: 1.268 ± 0.029
4.146ValIle: 4.146 ± 0.061
3.149ValLys: 3.149 ± 0.058
7.148ValLeu: 7.148 ± 0.073
1.664ValMet: 1.664 ± 0.038
2.711ValAsn: 2.711 ± 0.046
2.723ValPro: 2.723 ± 0.042
2.325ValGln: 2.325 ± 0.044
3.233ValArg: 3.233 ± 0.049
4.518ValSer: 4.518 ± 0.065
3.587ValThr: 3.587 ± 0.062
4.873ValVal: 4.873 ± 0.075
0.844ValTrp: 0.844 ± 0.024
1.775ValTyr: 1.775 ± 0.033
0.0ValXaa: 0.0 ± 0.0
Trp
0.96TrpAla: 0.96 ± 0.028
0.132TrpCys: 0.132 ± 0.01
0.625TrpAsp: 0.625 ± 0.023
0.642TrpGlu: 0.642 ± 0.02
0.585TrpPhe: 0.585 ± 0.021
0.754TrpGly: 0.754 ± 0.022
0.407TrpHis: 0.407 ± 0.014
0.714TrpIle: 0.714 ± 0.022
0.52TrpLys: 0.52 ± 0.022
2.135TrpLeu: 2.135 ± 0.047
0.292TrpMet: 0.292 ± 0.013
0.493TrpAsn: 0.493 ± 0.019
0.585TrpPro: 0.585 ± 0.023
0.968TrpGln: 0.968 ± 0.034
0.894TrpArg: 0.894 ± 0.03
0.752TrpSer: 0.752 ± 0.023
0.585TrpThr: 0.585 ± 0.023
0.786TrpVal: 0.786 ± 0.024
0.193TrpTrp: 0.193 ± 0.012
0.339TrpTyr: 0.339 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.496TyrAla: 2.496 ± 0.04
0.394TyrCys: 0.394 ± 0.017
1.521TyrAsp: 1.521 ± 0.032
1.365TyrGlu: 1.365 ± 0.03
1.34TyrPhe: 1.34 ± 0.03
2.122TyrGly: 2.122 ± 0.042
0.637TyrHis: 0.637 ± 0.021
1.333TyrIle: 1.333 ± 0.031
1.139TyrLys: 1.139 ± 0.03
3.465TyrLeu: 3.465 ± 0.048
0.473TyrMet: 0.473 ± 0.02
0.962TyrAsn: 0.962 ± 0.029
1.405TyrPro: 1.405 ± 0.03
1.688TyrGln: 1.688 ± 0.031
2.061TyrArg: 2.061 ± 0.038
1.838TyrSer: 1.838 ± 0.036
1.289TyrThr: 1.289 ± 0.035
1.706TyrVal: 1.706 ± 0.039
0.491TyrTrp: 0.491 ± 0.017
0.919TyrTyr: 0.919 ± 0.028
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4463 proteins (1456984 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski