Mesobacillus subterraneus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Mesobacillus

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3465 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D6Z6E9|A0A0D6Z6E9_9BACI DNA repair helicase OS=Mesobacillus subterraneus OX=285983 GN=UB32_18005 PE=4 SV=1
MM1 pKa = 8.28DD2 pKa = 5.3YY3 pKa = 11.2DD4 pKa = 3.91LQFAGWGPDD13 pKa = 3.2YY14 pKa = 11.49GNALSFTDD22 pKa = 4.45LWITDD27 pKa = 3.5GGSNRR32 pKa = 11.84MGYY35 pKa = 10.45SNPEE39 pKa = 3.45YY40 pKa = 10.92DD41 pKa = 4.43KK42 pKa = 11.14LIKK45 pKa = 10.17AAQGEE50 pKa = 4.4LATDD54 pKa = 4.76EE55 pKa = 4.27KK56 pKa = 11.11AQWEE60 pKa = 4.21AQQDD64 pKa = 3.61AEE66 pKa = 4.71KK67 pKa = 10.91IMLAEE72 pKa = 5.1DD73 pKa = 3.46YY74 pKa = 10.95GLAPVYY80 pKa = 10.45QRR82 pKa = 11.84AANILLNPNVKK93 pKa = 10.09GLAIYY98 pKa = 10.34NYY100 pKa = 9.85GPDD103 pKa = 4.82YY104 pKa = 10.66SFQWITIEE112 pKa = 4.77GEE114 pKa = 3.88

Molecular weight: 12.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D6ZA77|A0A0D6ZA77_9BACI Metal ABC transporter permease OS=Mesobacillus subterraneus OX=285983 GN=UB32_07185 PE=3 SV=1
MM1 pKa = 7.35SLKK4 pKa = 10.67NYY6 pKa = 10.48AILLKK11 pKa = 10.4ILGPAVFLVPIFLKK25 pKa = 10.67GMGYY29 pKa = 9.68IKK31 pKa = 10.39SISPAIMFSLIAIGVVLLIAGNICEE56 pKa = 4.3AKK58 pKa = 10.23AIRR61 pKa = 11.84SGEE64 pKa = 3.69YY65 pKa = 9.22RR66 pKa = 11.84RR67 pKa = 11.84WRR69 pKa = 11.84RR70 pKa = 11.84RR71 pKa = 11.84IKK73 pKa = 10.67

Molecular weight: 8.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3465

0

3465

1023216

26

2277

295.3

33.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.436 ± 0.044

0.628 ± 0.012

5.055 ± 0.033

7.594 ± 0.042

4.596 ± 0.031

7.142 ± 0.041

1.99 ± 0.019

7.725 ± 0.041

7.01 ± 0.039

9.833 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.889 ± 0.02

4.257 ± 0.027

3.615 ± 0.024

3.659 ± 0.025

4.114 ± 0.032

5.89 ± 0.025

5.282 ± 0.026

6.896 ± 0.031

1.004 ± 0.015

3.386 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski