Amino acid dipepetide frequency for Mesobacillus subterraneus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.902AlaAla: 6.902 ± 0.109
0.519AlaCys: 0.519 ± 0.024
3.766AlaAsp: 3.766 ± 0.061
5.478AlaGlu: 5.478 ± 0.088
3.353AlaPhe: 3.353 ± 0.066
6.037AlaGly: 6.037 ± 0.097
1.231AlaHis: 1.231 ± 0.033
6.137AlaIle: 6.137 ± 0.091
4.986AlaLys: 4.986 ± 0.076
7.222AlaLeu: 7.222 ± 0.085
2.23AlaMet: 2.23 ± 0.047
2.955AlaAsn: 2.955 ± 0.05
2.058AlaPro: 2.058 ± 0.048
2.218AlaGln: 2.218 ± 0.052
2.957AlaArg: 2.957 ± 0.056
4.088AlaSer: 4.088 ± 0.072
3.541AlaThr: 3.541 ± 0.063
5.757AlaVal: 5.757 ± 0.087
0.636AlaTrp: 0.636 ± 0.026
2.287AlaTyr: 2.287 ± 0.049
0.0AlaXaa: 0.0 ± 0.0
Cys
0.392CysAla: 0.392 ± 0.02
0.078CysCys: 0.078 ± 0.009
0.362CysAsp: 0.362 ± 0.02
0.409CysGlu: 0.409 ± 0.02
0.239CysPhe: 0.239 ± 0.017
0.617CysGly: 0.617 ± 0.028
0.187CysHis: 0.187 ± 0.014
0.439CysIle: 0.439 ± 0.021
0.311CysLys: 0.311 ± 0.019
0.551CysLeu: 0.551 ± 0.023
0.149CysMet: 0.149 ± 0.012
0.254CysAsn: 0.254 ± 0.013
0.348CysPro: 0.348 ± 0.018
0.233CysGln: 0.233 ± 0.016
0.286CysArg: 0.286 ± 0.017
0.451CysSer: 0.451 ± 0.023
0.348CysThr: 0.348 ± 0.018
0.356CysVal: 0.356 ± 0.021
0.064CysTrp: 0.064 ± 0.007
0.204CysTyr: 0.204 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
3.348AspAla: 3.348 ± 0.06
0.341AspCys: 0.341 ± 0.018
2.35AspAsp: 2.35 ± 0.046
4.069AspGlu: 4.069 ± 0.075
2.506AspPhe: 2.506 ± 0.053
3.583AspGly: 3.583 ± 0.072
1.187AspHis: 1.187 ± 0.033
3.819AspIle: 3.819 ± 0.069
3.031AspLys: 3.031 ± 0.049
5.145AspLeu: 5.145 ± 0.082
1.32AspMet: 1.32 ± 0.035
1.707AspAsn: 1.707 ± 0.044
2.082AspPro: 2.082 ± 0.049
2.102AspGln: 2.102 ± 0.046
2.399AspArg: 2.399 ± 0.051
2.778AspSer: 2.778 ± 0.047
2.325AspThr: 2.325 ± 0.046
3.684AspVal: 3.684 ± 0.06
0.659AspTrp: 0.659 ± 0.025
2.116AspTyr: 2.116 ± 0.045
0.0AspXaa: 0.0 ± 0.0
Glu
5.687GluAla: 5.687 ± 0.091
0.37GluCys: 0.37 ± 0.019
3.648GluAsp: 3.648 ± 0.063
6.81GluGlu: 6.81 ± 0.114
2.804GluPhe: 2.804 ± 0.055
4.395GluGly: 4.395 ± 0.07
1.376GluHis: 1.376 ± 0.038
5.876GluIle: 5.876 ± 0.082
6.967GluLys: 6.967 ± 0.089
7.339GluLeu: 7.339 ± 0.097
2.559GluMet: 2.559 ± 0.057
3.703GluAsn: 3.703 ± 0.059
1.928GluPro: 1.928 ± 0.041
3.366GluGln: 3.366 ± 0.061
3.472GluArg: 3.472 ± 0.068
3.44GluSer: 3.44 ± 0.063
3.987GluThr: 3.987 ± 0.067
5.069GluVal: 5.069 ± 0.064
0.798GluTrp: 0.798 ± 0.028
2.344GluTyr: 2.344 ± 0.056
0.0GluXaa: 0.0 ± 0.0
Phe
3.358PheAla: 3.358 ± 0.067
0.286PheCys: 0.286 ± 0.018
2.296PheAsp: 2.296 ± 0.049
2.959PheGlu: 2.959 ± 0.054
2.52PhePhe: 2.52 ± 0.065
3.42PheGly: 3.42 ± 0.057
1.089PheHis: 1.089 ± 0.033
3.666PheIle: 3.666 ± 0.067
2.434PheLys: 2.434 ± 0.047
4.935PheLeu: 4.935 ± 0.086
1.223PheMet: 1.223 ± 0.036
1.745PheAsn: 1.745 ± 0.041
1.724PhePro: 1.724 ± 0.047
1.633PheGln: 1.633 ± 0.043
1.616PheArg: 1.616 ± 0.041
3.242PheSer: 3.242 ± 0.06
2.579PheThr: 2.579 ± 0.05
3.016PheVal: 3.016 ± 0.059
0.509PheTrp: 0.509 ± 0.025
1.709PheTyr: 1.709 ± 0.044
0.0PheXaa: 0.0 ± 0.0
Gly
5.065GlyAla: 5.065 ± 0.084
0.607GlyCys: 0.607 ± 0.023
3.203GlyAsp: 3.203 ± 0.061
4.72GlyGlu: 4.72 ± 0.065
3.463GlyPhe: 3.463 ± 0.063
4.996GlyGly: 4.996 ± 0.089
1.432GlyHis: 1.432 ± 0.042
6.324GlyIle: 6.324 ± 0.084
5.413GlyLys: 5.413 ± 0.076
6.862GlyLeu: 6.862 ± 0.078
2.404GlyMet: 2.404 ± 0.053
2.85GlyAsn: 2.85 ± 0.052
1.83GlyPro: 1.83 ± 0.046
2.313GlyGln: 2.313 ± 0.049
2.855GlyArg: 2.855 ± 0.053
4.172GlySer: 4.172 ± 0.073
4.196GlyThr: 4.196 ± 0.08
5.046GlyVal: 5.046 ± 0.075
0.793GlyTrp: 0.793 ± 0.029
2.878GlyTyr: 2.878 ± 0.06
0.0GlyXaa: 0.0 ± 0.0
His
1.255HisAla: 1.255 ± 0.036
0.194HisCys: 0.194 ± 0.013
0.996HisAsp: 0.996 ± 0.031
1.341HisGlu: 1.341 ± 0.039
1.054HisPhe: 1.054 ± 0.029
1.421HisGly: 1.421 ± 0.039
0.59HisHis: 0.59 ± 0.026
1.392HisIle: 1.392 ± 0.035
1.051HisLys: 1.051 ± 0.034
2.103HisLeu: 2.103 ± 0.049
0.514HisMet: 0.514 ± 0.025
0.679HisAsn: 0.679 ± 0.027
1.133HisPro: 1.133 ± 0.036
0.822HisGln: 0.822 ± 0.03
0.837HisArg: 0.837 ± 0.032
1.181HisSer: 1.181 ± 0.039
1.044HisThr: 1.044 ± 0.029
1.291HisVal: 1.291 ± 0.034
0.192HisTrp: 0.192 ± 0.014
0.812HisTyr: 0.812 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.242IleAla: 6.242 ± 0.094
0.472IleCys: 0.472 ± 0.023
4.227IleAsp: 4.227 ± 0.063
5.548IleGlu: 5.548 ± 0.079
3.333IlePhe: 3.333 ± 0.076
6.177IleGly: 6.177 ± 0.089
1.68IleHis: 1.68 ± 0.037
5.626IleIle: 5.626 ± 0.084
4.706IleLys: 4.706 ± 0.069
7.342IleLeu: 7.342 ± 0.098
1.792IleMet: 1.792 ± 0.04
3.13IleAsn: 3.13 ± 0.062
3.35IlePro: 3.35 ± 0.065
2.815IleGln: 2.815 ± 0.048
3.167IleArg: 3.167 ± 0.055
5.046IleSer: 5.046 ± 0.059
4.126IleThr: 4.126 ± 0.073
5.492IleVal: 5.492 ± 0.087
0.628IleTrp: 0.628 ± 0.025
2.351IleTyr: 2.351 ± 0.048
0.0IleXaa: 0.0 ± 0.0
Lys
5.049LysAla: 5.049 ± 0.07
0.324LysCys: 0.324 ± 0.019
3.847LysAsp: 3.847 ± 0.069
6.664LysGlu: 6.664 ± 0.093
2.079LysPhe: 2.079 ± 0.043
4.631LysGly: 4.631 ± 0.066
1.2LysHis: 1.2 ± 0.034
5.098LysIle: 5.098 ± 0.067
5.956LysLys: 5.956 ± 0.083
6.194LysLeu: 6.194 ± 0.069
2.352LysMet: 2.352 ± 0.045
3.412LysAsn: 3.412 ± 0.05
2.289LysPro: 2.289 ± 0.051
3.034LysGln: 3.034 ± 0.06
3.1LysArg: 3.1 ± 0.054
3.443LysSer: 3.443 ± 0.056
3.726LysThr: 3.726 ± 0.064
4.694LysVal: 4.694 ± 0.076
0.829LysTrp: 0.829 ± 0.03
2.182LysTyr: 2.182 ± 0.047
0.0LysXaa: 0.0 ± 0.0
Leu
7.98LeuAla: 7.98 ± 0.092
0.509LeuCys: 0.509 ± 0.024
4.946LeuAsp: 4.946 ± 0.07
7.017LeuGlu: 7.017 ± 0.09
4.813LeuPhe: 4.813 ± 0.071
6.599LeuGly: 6.599 ± 0.085
1.907LeuHis: 1.907 ± 0.047
7.115LeuIle: 7.115 ± 0.113
6.876LeuLys: 6.876 ± 0.091
10.089LeuLeu: 10.089 ± 0.134
2.621LeuMet: 2.621 ± 0.048
4.071LeuAsn: 4.071 ± 0.061
4.049LeuPro: 4.049 ± 0.065
3.576LeuGln: 3.576 ± 0.067
3.678LeuArg: 3.678 ± 0.068
6.676LeuSer: 6.676 ± 0.093
5.606LeuThr: 5.606 ± 0.066
6.394LeuVal: 6.394 ± 0.081
0.811LeuTrp: 0.811 ± 0.029
2.996LeuTyr: 2.996 ± 0.065
0.0LeuXaa: 0.0 ± 0.0
Met
2.239MetAla: 2.239 ± 0.048
0.142MetCys: 0.142 ± 0.012
1.583MetAsp: 1.583 ± 0.041
2.429MetGlu: 2.429 ± 0.046
1.145MetPhe: 1.145 ± 0.036
1.887MetGly: 1.887 ± 0.044
0.415MetHis: 0.415 ± 0.022
2.235MetIle: 2.235 ± 0.043
2.541MetLys: 2.541 ± 0.05
2.664MetLeu: 2.664 ± 0.051
0.883MetMet: 0.883 ± 0.032
1.618MetAsn: 1.618 ± 0.041
1.047MetPro: 1.047 ± 0.036
0.868MetGln: 0.868 ± 0.031
1.1MetArg: 1.1 ± 0.034
1.635MetSer: 1.635 ± 0.037
1.654MetThr: 1.654 ± 0.045
1.92MetVal: 1.92 ± 0.04
0.184MetTrp: 0.184 ± 0.014
0.704MetTyr: 0.704 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
2.861AsnAla: 2.861 ± 0.066
0.26AsnCys: 0.26 ± 0.015
2.067AsnAsp: 2.067 ± 0.043
3.361AsnGlu: 3.361 ± 0.055
1.766AsnPhe: 1.766 ± 0.037
3.533AsnGly: 3.533 ± 0.062
0.93AsnHis: 0.93 ± 0.031
3.132AsnIle: 3.132 ± 0.058
2.774AsnLys: 2.774 ± 0.059
3.894AsnLeu: 3.894 ± 0.061
1.207AsnMet: 1.207 ± 0.035
1.742AsnAsn: 1.742 ± 0.043
2.181AsnPro: 2.181 ± 0.05
1.748AsnGln: 1.748 ± 0.045
2.062AsnArg: 2.062 ± 0.053
2.344AsnSer: 2.344 ± 0.045
1.989AsnThr: 1.989 ± 0.047
2.755AsnVal: 2.755 ± 0.05
0.547AsnTrp: 0.547 ± 0.025
1.416AsnTyr: 1.416 ± 0.037
0.0AsnXaa: 0.0 ± 0.0
Pro
2.562ProAla: 2.562 ± 0.054
0.189ProCys: 0.189 ± 0.014
2.142ProAsp: 2.142 ± 0.05
3.271ProGlu: 3.271 ± 0.061
1.987ProPhe: 1.987 ± 0.05
2.665ProGly: 2.665 ± 0.056
0.714ProHis: 0.714 ± 0.027
2.713ProIle: 2.713 ± 0.056
2.164ProLys: 2.164 ± 0.05
3.488ProLeu: 3.488 ± 0.062
0.853ProMet: 0.853 ± 0.031
1.615ProAsn: 1.615 ± 0.048
0.973ProPro: 0.973 ± 0.031
1.141ProGln: 1.141 ± 0.033
1.166ProArg: 1.166 ± 0.035
2.065ProSer: 2.065 ± 0.045
1.767ProThr: 1.767 ± 0.044
2.938ProVal: 2.938 ± 0.062
0.324ProTrp: 0.324 ± 0.019
1.412ProTyr: 1.412 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
2.808GlnAla: 2.808 ± 0.054
0.167GlnCys: 0.167 ± 0.013
1.661GlnAsp: 1.661 ± 0.04
2.887GlnGlu: 2.887 ± 0.056
1.645GlnPhe: 1.645 ± 0.044
2.193GlnGly: 2.193 ± 0.047
0.737GlnHis: 0.737 ± 0.031
2.564GlnIle: 2.564 ± 0.046
2.706GlnLys: 2.706 ± 0.058
3.98GlnLeu: 3.98 ± 0.069
1.154GlnMet: 1.154 ± 0.03
1.612GlnAsn: 1.612 ± 0.043
1.24GlnPro: 1.24 ± 0.036
1.777GlnGln: 1.777 ± 0.049
1.427GlnArg: 1.427 ± 0.034
2.03GlnSer: 2.03 ± 0.045
2.003GlnThr: 2.003 ± 0.047
2.296GlnVal: 2.296 ± 0.053
0.387GlnTrp: 0.387 ± 0.021
1.314GlnTyr: 1.314 ± 0.038
0.0GlnXaa: 0.0 ± 0.0
Arg
2.473ArgAla: 2.473 ± 0.045
0.24ArgCys: 0.24 ± 0.016
2.089ArgAsp: 2.089 ± 0.046
3.425ArgGlu: 3.425 ± 0.063
1.976ArgPhe: 1.976 ± 0.05
2.452ArgGly: 2.452 ± 0.051
0.766ArgHis: 0.766 ± 0.03
3.137ArgIle: 3.137 ± 0.062
3.471ArgLys: 3.471 ± 0.062
4.103ArgLeu: 4.103 ± 0.06
1.363ArgMet: 1.363 ± 0.036
1.995ArgAsn: 1.995 ± 0.038
1.365ArgPro: 1.365 ± 0.041
1.542ArgGln: 1.542 ± 0.043
1.876ArgArg: 1.876 ± 0.045
2.326ArgSer: 2.326 ± 0.048
2.085ArgThr: 2.085 ± 0.049
2.601ArgVal: 2.601 ± 0.057
0.398ArgTrp: 0.398 ± 0.021
1.452ArgTyr: 1.452 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
4.07SerAla: 4.07 ± 0.073
0.386SerCys: 0.386 ± 0.019
2.713SerAsp: 2.713 ± 0.049
3.923SerGlu: 3.923 ± 0.073
3.219SerPhe: 3.219 ± 0.067
4.635SerGly: 4.635 ± 0.081
1.169SerHis: 1.169 ± 0.036
4.858SerIle: 4.858 ± 0.075
3.78SerLys: 3.78 ± 0.06
6.139SerLeu: 6.139 ± 0.093
1.796SerMet: 1.796 ± 0.042
2.385SerAsn: 2.385 ± 0.048
2.065SerPro: 2.065 ± 0.045
1.887SerGln: 1.887 ± 0.044
2.491SerArg: 2.491 ± 0.048
3.7SerSer: 3.7 ± 0.07
2.951SerThr: 2.951 ± 0.058
4.026SerVal: 4.026 ± 0.058
0.625SerTrp: 0.625 ± 0.024
2.079SerTyr: 2.079 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
4.319ThrAla: 4.319 ± 0.07
0.334ThrCys: 0.334 ± 0.019
2.604ThrAsp: 2.604 ± 0.053
3.715ThrGlu: 3.715 ± 0.059
2.507ThrPhe: 2.507 ± 0.05
4.453ThrGly: 4.453 ± 0.068
0.927ThrHis: 0.927 ± 0.032
4.394ThrIle: 4.394 ± 0.071
3.236ThrLys: 3.236 ± 0.06
4.949ThrLeu: 4.949 ± 0.077
1.398ThrMet: 1.398 ± 0.036
2.281ThrAsn: 2.281 ± 0.056
2.123ThrPro: 2.123 ± 0.05
1.339ThrGln: 1.339 ± 0.033
1.904ThrArg: 1.904 ± 0.045
3.149ThrSer: 3.149 ± 0.053
2.745ThrThr: 2.745 ± 0.064
4.248ThrVal: 4.248 ± 0.064
0.465ThrTrp: 0.465 ± 0.021
1.734ThrTyr: 1.734 ± 0.05
0.0ThrXaa: 0.0 ± 0.0
Val
5.037ValAla: 5.037 ± 0.082
0.536ValCys: 0.536 ± 0.027
3.702ValAsp: 3.702 ± 0.066
4.779ValGlu: 4.779 ± 0.075
3.246ValPhe: 3.246 ± 0.062
4.572ValGly: 4.572 ± 0.074
1.328ValHis: 1.328 ± 0.038
5.576ValIle: 5.576 ± 0.078
4.826ValLys: 4.826 ± 0.075
6.728ValLeu: 6.728 ± 0.079
1.879ValMet: 1.879 ± 0.043
2.986ValAsn: 2.986 ± 0.058
2.73ValPro: 2.73 ± 0.053
2.266ValGln: 2.266 ± 0.045
2.713ValArg: 2.713 ± 0.047
4.443ValSer: 4.443 ± 0.073
3.978ValThr: 3.978 ± 0.067
4.77ValVal: 4.77 ± 0.066
0.644ValTrp: 0.644 ± 0.026
2.219ValTyr: 2.219 ± 0.046
0.0ValXaa: 0.0 ± 0.0
Trp
0.628TrpAla: 0.628 ± 0.028
0.051TrpCys: 0.051 ± 0.007
0.474TrpAsp: 0.474 ± 0.023
0.67TrpGlu: 0.67 ± 0.027
0.502TrpPhe: 0.502 ± 0.023
0.696TrpGly: 0.696 ± 0.025
0.199TrpHis: 0.199 ± 0.014
0.833TrpIle: 0.833 ± 0.036
0.794TrpLys: 0.794 ± 0.026
1.098TrpLeu: 1.098 ± 0.037
0.344TrpMet: 0.344 ± 0.02
0.545TrpAsn: 0.545 ± 0.023
0.274TrpPro: 0.274 ± 0.018
0.367TrpGln: 0.367 ± 0.019
0.406TrpArg: 0.406 ± 0.02
0.585TrpSer: 0.585 ± 0.023
0.498TrpThr: 0.498 ± 0.021
0.565TrpVal: 0.565 ± 0.025
0.17TrpTrp: 0.17 ± 0.013
0.34TrpTyr: 0.34 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.087TyrAla: 2.087 ± 0.044
0.269TyrCys: 0.269 ± 0.017
1.876TyrAsp: 1.876 ± 0.05
2.464TyrGlu: 2.464 ± 0.046
1.805TyrPhe: 1.805 ± 0.048
2.46TyrGly: 2.46 ± 0.049
0.834TyrHis: 0.834 ± 0.026
2.318TyrIle: 2.318 ± 0.052
2.081TyrLys: 2.081 ± 0.045
3.505TyrLeu: 3.505 ± 0.059
0.85TyrMet: 0.85 ± 0.025
1.284TyrAsn: 1.284 ± 0.033
1.36TyrPro: 1.36 ± 0.033
1.54TyrGln: 1.54 ± 0.039
1.622TyrArg: 1.622 ± 0.042
2.105TyrSer: 2.105 ± 0.048
1.676TyrThr: 1.676 ± 0.047
2.039TyrVal: 2.039 ± 0.046
0.376TyrTrp: 0.376 ± 0.021
1.307TyrTyr: 1.307 ± 0.039
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3465 proteins (1023216 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski