East African cassava mosaic virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U3LY20|U3LY20_9GEMI Replication-associated protein OS=East African cassava mosaic virus OX=62079 GN=AC1 PE=3 SV=1
MM1 pKa = 8.09WDD3 pKa = 3.51PLLNDD8 pKa = 3.72FPEE11 pKa = 4.7TVHH14 pKa = 6.61GFRR17 pKa = 11.84SMLAVKK23 pKa = 10.21YY24 pKa = 10.06LLHH27 pKa = 7.24LEE29 pKa = 4.05QEE31 pKa = 4.43YY32 pKa = 11.26DD33 pKa = 3.21RR34 pKa = 11.84GTVGAEE40 pKa = 4.21YY41 pKa = 9.66IWDD44 pKa = 4.8LIGVLRR50 pKa = 11.84CEE52 pKa = 4.68SYY54 pKa = 10.9VEE56 pKa = 3.65ATRR59 pKa = 11.84RR60 pKa = 11.84YY61 pKa = 9.31NNLNTRR67 pKa = 11.84IQGAEE72 pKa = 3.96EE73 pKa = 4.16AEE75 pKa = 4.04LRR77 pKa = 11.84QPIQEE82 pKa = 4.26PCCCPHH88 pKa = 7.07CPRR91 pKa = 11.84HH92 pKa = 5.47QKK94 pKa = 10.61QNMGQQAHH102 pKa = 5.32VSEE105 pKa = 4.72AQDD108 pKa = 3.39VQNVSKK114 pKa = 10.24PRR116 pKa = 11.84CPP118 pKa = 3.47

Molecular weight: 13.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U3LZP4|U3LZP4_9GEMI Replication enhancer OS=East African cassava mosaic virus OX=62079 GN=AC3 PE=3 SV=1
MM1 pKa = 7.98RR2 pKa = 11.84YY3 pKa = 9.59SSPSQSHH10 pKa = 6.46SSPPPIKK17 pKa = 10.22ARR19 pKa = 11.84HH20 pKa = 5.05RR21 pKa = 11.84QAKK24 pKa = 8.58IRR26 pKa = 11.84APRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84IDD34 pKa = 3.62LSCGCSIYY42 pKa = 10.64RR43 pKa = 11.84SINCHH48 pKa = 3.71NHH50 pKa = 5.17GFTHH54 pKa = 7.48RR55 pKa = 11.84GTHH58 pKa = 5.82HH59 pKa = 6.87CSSSAEE65 pKa = 3.53WRR67 pKa = 11.84IYY69 pKa = 10.29MGDD72 pKa = 3.26TKK74 pKa = 11.22SPIFQNPQPQQQAVQHH90 pKa = 5.86EE91 pKa = 4.68PRR93 pKa = 11.84HH94 pKa = 5.39HH95 pKa = 6.78RR96 pKa = 11.84CPDD99 pKa = 3.32SVQLQLNKK107 pKa = 10.3EE108 pKa = 4.39HH109 pKa = 7.14ILFHH113 pKa = 7.34DD114 pKa = 4.24FLIQTGYY121 pKa = 8.55PQKK124 pKa = 10.61LYY126 pKa = 10.58SIHH129 pKa = 7.64DD130 pKa = 4.13ALHH133 pKa = 6.28LLL135 pKa = 4.05

Molecular weight: 15.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

996

118

352

199.2

23.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.12 ± 0.88

2.51 ± 0.571

4.719 ± 0.257

4.719 ± 0.903

4.618 ± 0.742

4.618 ± 0.513

4.116 ± 1.265

6.627 ± 0.953

5.422 ± 0.814

6.124 ± 0.761

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.41 ± 0.595

5.723 ± 0.75

6.827 ± 0.258

5.422 ± 0.971

7.329 ± 1.047

8.133 ± 1.719

4.418 ± 0.501

4.819 ± 1.266

1.305 ± 0.305

5.02 ± 0.467
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski