Acidianus bottle-shaped virus (isolate Italy/Pozzuoli) (ABV)

Taxonomy: Viruses; Ampullaviridae; Bottigliavirus; Bottigliavirus ABV; Acidianus bottle-shaped virus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|A4ZUE0|Y117_ABVP Uncharacterized protein OR117 OS=Acidianus bottle-shaped virus (isolate Italy/Pozzuoli) OX=654911 GN=OR117 PE=4 SV=1
MM1 pKa = 7.49VIGNSVDD8 pKa = 4.27LLIDD12 pKa = 4.92LINEE16 pKa = 4.04NFDD19 pKa = 4.11GNDD22 pKa = 3.13NAYY25 pKa = 9.74KK26 pKa = 10.72NLVRR30 pKa = 11.84YY31 pKa = 9.45ILRR34 pKa = 11.84LIDD37 pKa = 4.23CSVTDD42 pKa = 3.47IQDD45 pKa = 3.44SVFMFKK51 pKa = 10.02QQSDD55 pKa = 3.68TDD57 pKa = 3.84DD58 pKa = 4.7SEE60 pKa = 5.63DD61 pKa = 3.43NN62 pKa = 3.75

Molecular weight: 7.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|A4ZUA9|Y061A_ABVP Uncharacterized protein ORF61a OS=Acidianus bottle-shaped virus (isolate Italy/Pozzuoli) OX=654911 GN=ORF61a PE=4 SV=1
MM1 pKa = 7.69ANFLGISDD9 pKa = 5.25LLGQILKK16 pKa = 10.41ALTRR20 pKa = 11.84ITFDD24 pKa = 4.14VLFTLAKK31 pKa = 9.46YY32 pKa = 9.42VIRR35 pKa = 11.84GFGHH39 pKa = 7.22WIMDD43 pKa = 3.68TFRR46 pKa = 11.84YY47 pKa = 10.58LNDD50 pKa = 2.99FTVWITRR57 pKa = 11.84LAKK60 pKa = 10.25RR61 pKa = 4.0

Molecular weight: 7.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

2

56

7714

37

653

137.8

15.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.576 ± 0.277

1.167 ± 0.255

4.991 ± 0.33

6.741 ± 0.521

5.561 ± 0.255

5.043 ± 0.585

1.27 ± 0.141

8.75 ± 0.352

7.778 ± 0.56

10.682 ± 0.587

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.476 ± 0.214

5.821 ± 0.306

3.993 ± 0.384

3.526 ± 0.311

3.565 ± 0.29

6.534 ± 0.401

5.185 ± 0.319

5.963 ± 0.276

0.622 ± 0.075

5.743 ± 0.353
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski