Salmonella phage g341c
Average proteome isoelectric point is 7.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C6ZR59|C6ZR59_9CAUD Hypothetical phage protein OS=Salmonella phage g341c OX=590739 GN=53 PE=4 SV=1
MM1 pKa = 7.82 IDD3 pKa = 3.4 PNRR6 pKa = 11.84 SYY8 pKa = 10.61 EE9 pKa = 3.97 QEE11 pKa = 4.12 SVEE14 pKa = 5.1 RR15 pKa = 11.84 ALTCANCGQKK25 pKa = 10.32 LHH27 pKa = 5.86 VLEE30 pKa = 4.31 VHH32 pKa = 5.94 VCEE35 pKa = 4.54 HH36 pKa = 6.71 CCAEE40 pKa = 5.41 LMSDD44 pKa = 4.6 PNNSMYY50 pKa = 10.74 EE51 pKa = 4.03 EE52 pKa = 4.59 EE53 pKa = 5.1 DD54 pKa = 3.95 DD55 pKa = 4.1 EE56 pKa = 4.75
Molecular weight: 6.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.082
IPC2_protein 4.266
IPC_protein 4.126
Toseland 3.986
ProMoST 4.177
Dawson 4.075
Bjellqvist 4.291
Wikipedia 3.935
Rodwell 3.973
Grimsley 3.897
Solomon 4.062
Lehninger 4.012
Nozaki 4.19
DTASelect 4.279
Thurlkill 3.999
EMBOSS 3.961
Sillero 4.24
Patrickios 0.362
IPC_peptide 4.062
IPC2_peptide 4.228
IPC2.peptide.svr19 4.189
Protein with the highest isoelectric point:
>tr|C6ZR58|C6ZR58_9CAUD NinF protein OS=Salmonella phage g341c OX=590739 GN=ninF PE=4 SV=1
MM1 pKa = 7.39 ARR3 pKa = 11.84 QRR5 pKa = 11.84 RR6 pKa = 11.84 SITDD10 pKa = 4.1 IICEE14 pKa = 3.96 NCKK17 pKa = 10.21 YY18 pKa = 10.77 LPTKK22 pKa = 10.48 RR23 pKa = 11.84 SRR25 pKa = 11.84 NKK27 pKa = 9.55 PKK29 pKa = 10.29 PIPKK33 pKa = 9.96 EE34 pKa = 3.93 SDD36 pKa = 3.19 VKK38 pKa = 9.6 TFNYY42 pKa = 7.51 TAHH45 pKa = 6.73 LWDD48 pKa = 3.61 IRR50 pKa = 11.84 WLRR53 pKa = 11.84 EE54 pKa = 3.55 RR55 pKa = 11.84 ARR57 pKa = 11.84 KK58 pKa = 7.22 TRR60 pKa = 3.32
Molecular weight: 7.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.333
IPC2_protein 9.765
IPC_protein 10.584
Toseland 10.935
ProMoST 10.994
Dawson 10.994
Bjellqvist 10.716
Wikipedia 11.213
Rodwell 11.257
Grimsley 11.038
Solomon 11.169
Lehninger 11.125
Nozaki 10.921
DTASelect 10.716
Thurlkill 10.921
EMBOSS 11.345
Sillero 10.935
Patrickios 11.008
IPC_peptide 11.169
IPC2_peptide 9.78
IPC2.peptide.svr19 8.718
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
68
0
68
13109
35
792
192.8
21.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.017 ± 0.475
1.335 ± 0.143
5.561 ± 0.262
6.309 ± 0.319
3.86 ± 0.21
7.316 ± 0.278
1.892 ± 0.167
5.92 ± 0.349
5.988 ± 0.265
7.636 ± 0.259
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.838 ± 0.143
4.646 ± 0.185
4.272 ± 0.172
4.821 ± 0.461
5.859 ± 0.293
6.667 ± 0.384
4.936 ± 0.27
5.965 ± 0.268
1.541 ± 0.137
3.623 ± 0.281
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here