Gluconacetobacter sp. SXCC-1
Average proteome isoelectric point is 7.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4747 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F3S3N1|F3S3N1_9PROT Uncharacterized protein OS=Gluconacetobacter sp. SXCC-1 OX=1004836 GN=SXCC_00652 PE=4 SV=1
MM1 pKa = 7.58 ANSLYY6 pKa = 10.93 SVFLQQALTKK16 pKa = 10.71 NIDD19 pKa = 3.45 VTDD22 pKa = 4.23 GSIRR26 pKa = 11.84 LALLDD31 pKa = 3.51 NSYY34 pKa = 11.45 SFNAGDD40 pKa = 4.77 ADD42 pKa = 3.91 TTSALKK48 pKa = 10.69 ALVGAAVAPSAAVTVAGGSVTIPAATFAAIASGTTVQAVLVYY90 pKa = 10.88 SGTTPIAYY98 pKa = 8.96 IDD100 pKa = 3.8 TATGIPASTDD110 pKa = 2.61 GSTITVSWTAAAITLGLTASS130 pKa = 3.68
Molecular weight: 12.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.884
IPC_protein 3.77
Toseland 3.554
ProMoST 4.024
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.872
Rodwell 3.617
Grimsley 3.478
Solomon 3.783
Lehninger 3.745
Nozaki 3.973
DTASelect 4.291
Thurlkill 3.668
EMBOSS 3.859
Sillero 3.91
Patrickios 1.952
IPC_peptide 3.77
IPC2_peptide 3.872
IPC2.peptide.svr19 3.827
Protein with the highest isoelectric point:
>tr|F3S211|F3S211_9PROT Uncharacterized protein OS=Gluconacetobacter sp. SXCC-1 OX=1004836 GN=SXCC_00078 PE=4 SV=1
MM1 pKa = 7.68 GKK3 pKa = 9.65 RR4 pKa = 11.84 AGRR7 pKa = 11.84 RR8 pKa = 11.84 TMSPTRR14 pKa = 11.84 TILPNPKK21 pKa = 8.12 NTKK24 pKa = 9.01 RR25 pKa = 11.84 KK26 pKa = 9.46 KK27 pKa = 10.57 GKK29 pKa = 9.74 ALPWRR34 pKa = 11.84 AGRR37 pKa = 11.84 GQRR40 pKa = 11.84 WRR42 pKa = 11.84 FPMLTMLRR50 pKa = 11.84 VGPVPAQGAALAPTGTPWPRR70 pKa = 11.84 AKK72 pKa = 10.4 HH73 pKa = 5.56 PKK75 pKa = 9.28 QKK77 pKa = 10.93
Molecular weight: 8.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.521
IPC2_protein 11.272
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.778
Grimsley 13.056
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.501
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.163
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4747
0
4747
1206339
37
3750
254.1
27.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.123 ± 0.054
1.173 ± 0.016
5.53 ± 0.029
4.65 ± 0.039
3.445 ± 0.027
8.687 ± 0.038
2.704 ± 0.02
4.872 ± 0.031
2.334 ± 0.029
9.951 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.978 ± 0.021
2.585 ± 0.031
5.875 ± 0.033
3.405 ± 0.025
7.783 ± 0.052
5.284 ± 0.04
5.732 ± 0.047
7.193 ± 0.032
1.451 ± 0.016
2.247 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here