Halogeometricum pallidum JCM 14848

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Haloferacaceae; Halogeometricum; Halogeometricum pallidum

Average proteome isoelectric point is 4.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4246 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|M0CWZ2|M0CWZ2_9EURY Acetolactate synthase small subunit OS=Halogeometricum pallidum JCM 14848 OX=1227487 GN=C474_19689 PE=3 SV=1
MM1 pKa = 7.44EE2 pKa = 4.86LTWHH6 pKa = 6.23GHH8 pKa = 4.56STWHH12 pKa = 6.21VDD14 pKa = 3.02VDD16 pKa = 3.79GTTFLIDD23 pKa = 3.77PFFDD27 pKa = 4.12NPFTDD32 pKa = 4.86LSPSDD37 pKa = 4.69VEE39 pKa = 4.85DD40 pKa = 4.27PDD42 pKa = 4.37YY43 pKa = 11.66LLLTHH48 pKa = 6.35SHH50 pKa = 7.13ADD52 pKa = 3.61HH53 pKa = 6.9ISDD56 pKa = 4.51AGAFTDD62 pKa = 3.61ATVVGVPEE70 pKa = 3.95LTGYY74 pKa = 9.41MEE76 pKa = 4.87DD77 pKa = 3.84EE78 pKa = 4.44EE79 pKa = 5.89GFTDD83 pKa = 4.91SIGMNMGGTVEE94 pKa = 4.73CDD96 pKa = 3.03DD97 pKa = 5.26AFVTMHH103 pKa = 7.18RR104 pKa = 11.84ADD106 pKa = 3.55HH107 pKa = 6.09TNGLNTSYY115 pKa = 11.04EE116 pKa = 3.92NDD118 pKa = 3.16FGVPVGFLLSDD129 pKa = 3.91KK130 pKa = 10.97KK131 pKa = 9.96PTQEE135 pKa = 3.9SDD137 pKa = 2.94ADD139 pKa = 3.42ATTFYY144 pKa = 10.73HH145 pKa = 7.26AGDD148 pKa = 3.7TGLMSEE154 pKa = 4.58MKK156 pKa = 10.45DD157 pKa = 3.37VIGPYY162 pKa = 10.08LEE164 pKa = 5.01PDD166 pKa = 3.44AAALPCGDD174 pKa = 4.89HH175 pKa = 5.95FTMGPVQAAIAVDD188 pKa = 3.72WLDD191 pKa = 3.34VDD193 pKa = 4.28HH194 pKa = 7.44AFPMHH199 pKa = 7.07YY200 pKa = 8.84DD201 pKa = 3.67TFPPVEE207 pKa = 4.07IDD209 pKa = 2.98VDD211 pKa = 3.84DD212 pKa = 4.81FEE214 pKa = 6.63RR215 pKa = 11.84EE216 pKa = 4.13VKK218 pKa = 9.75ATGSDD223 pKa = 3.69AEE225 pKa = 4.36VHH227 pKa = 6.26VLDD230 pKa = 4.77GDD232 pKa = 3.82EE233 pKa = 4.38TFTLEE238 pKa = 4.13

Molecular weight:
26.07 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|M0D2Y9|M0D2Y9_9EURY DegT/DnrJ/EryC1/StrS aminotransferase OS=Halogeometricum pallidum JCM 14848 OX=1227487 GN=C474_15349 PE=3 SV=1
MM1 pKa = 7.1KK2 pKa = 10.41QQVLKK7 pKa = 10.97QFLGSKK13 pKa = 10.15KK14 pKa = 10.0SRR16 pKa = 11.84QLGVILPLVQAAFALLKK33 pKa = 10.3KK34 pKa = 10.05KK35 pKa = 10.14PKK37 pKa = 9.88RR38 pKa = 11.84ALLLVGVALVSSRR51 pKa = 11.84STKK54 pKa = 10.46LGTVAQVLLKK64 pKa = 10.83VFGGRR69 pKa = 11.84GSKK72 pKa = 10.16AA73 pKa = 2.96

Molecular weight:
7.8 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4246

0

4246

1220474

32

2489

287.4

31.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.028 ± 0.054

0.695 ± 0.011

8.085 ± 0.051

8.561 ± 0.055

3.467 ± 0.026

8.717 ± 0.039

1.937 ± 0.019

3.498 ± 0.029

1.89 ± 0.022

8.811 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.783 ± 0.014

2.416 ± 0.023

4.627 ± 0.023

2.242 ± 0.023

6.903 ± 0.045

5.767 ± 0.035

6.283 ± 0.032

9.382 ± 0.046

1.18 ± 0.016

2.724 ± 0.02

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski