Halogeometricum pallidum JCM 14848
Average proteome isoelectric point is 4.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4246 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M0CWZ2|M0CWZ2_9EURY Acetolactate synthase small subunit OS=Halogeometricum pallidum JCM 14848 OX=1227487 GN=C474_19689 PE=3 SV=1
MM1 pKa = 7.44 EE2 pKa = 4.86 LTWHH6 pKa = 6.23 GHH8 pKa = 4.56 STWHH12 pKa = 6.21 VDD14 pKa = 3.02 VDD16 pKa = 3.79 GTTFLIDD23 pKa = 3.77 PFFDD27 pKa = 4.12 NPFTDD32 pKa = 4.86 LSPSDD37 pKa = 4.69 VEE39 pKa = 4.85 DD40 pKa = 4.27 PDD42 pKa = 4.37 YY43 pKa = 11.66 LLLTHH48 pKa = 6.35 SHH50 pKa = 7.13 ADD52 pKa = 3.61 HH53 pKa = 6.9 ISDD56 pKa = 4.51 AGAFTDD62 pKa = 3.61 ATVVGVPEE70 pKa = 3.95 LTGYY74 pKa = 9.41 MEE76 pKa = 4.87 DD77 pKa = 3.84 EE78 pKa = 4.44 EE79 pKa = 5.89 GFTDD83 pKa = 4.91 SIGMNMGGTVEE94 pKa = 4.73 CDD96 pKa = 3.03 DD97 pKa = 5.26 AFVTMHH103 pKa = 7.18 RR104 pKa = 11.84 ADD106 pKa = 3.55 HH107 pKa = 6.09 TNGLNTSYY115 pKa = 11.04 EE116 pKa = 3.92 NDD118 pKa = 3.16 FGVPVGFLLSDD129 pKa = 3.91 KK130 pKa = 10.97 KK131 pKa = 9.96 PTQEE135 pKa = 3.9 SDD137 pKa = 2.94 ADD139 pKa = 3.42 ATTFYY144 pKa = 10.73 HH145 pKa = 7.26 AGDD148 pKa = 3.7 TGLMSEE154 pKa = 4.58 MKK156 pKa = 10.45 DD157 pKa = 3.37 VIGPYY162 pKa = 10.08 LEE164 pKa = 5.01 PDD166 pKa = 3.44 AAALPCGDD174 pKa = 4.89 HH175 pKa = 5.95 FTMGPVQAAIAVDD188 pKa = 3.72 WLDD191 pKa = 3.34 VDD193 pKa = 4.28 HH194 pKa = 7.44 AFPMHH199 pKa = 7.07 YY200 pKa = 8.84 DD201 pKa = 3.67 TFPPVEE207 pKa = 4.07 IDD209 pKa = 2.98 VDD211 pKa = 3.84 DD212 pKa = 4.81 FEE214 pKa = 6.63 RR215 pKa = 11.84 EE216 pKa = 4.13 VKK218 pKa = 9.75 ATGSDD223 pKa = 3.69 AEE225 pKa = 4.36 VHH227 pKa = 6.26 VLDD230 pKa = 4.77 GDD232 pKa = 3.82 EE233 pKa = 4.38 TFTLEE238 pKa = 4.13
Molecular weight: 26.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.682
IPC2_protein 3.77
IPC_protein 3.795
Toseland 3.579
ProMoST 3.948
Dawson 3.795
Bjellqvist 3.973
Wikipedia 3.745
Rodwell 3.63
Grimsley 3.478
Solomon 3.795
Lehninger 3.745
Nozaki 3.91
DTASelect 4.177
Thurlkill 3.63
EMBOSS 3.757
Sillero 3.923
Patrickios 1.278
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|M0D2Y9|M0D2Y9_9EURY DegT/DnrJ/EryC1/StrS aminotransferase OS=Halogeometricum pallidum JCM 14848 OX=1227487 GN=C474_15349 PE=3 SV=1
MM1 pKa = 7.1 KK2 pKa = 10.41 QQVLKK7 pKa = 10.97 QFLGSKK13 pKa = 10.15 KK14 pKa = 10.0 SRR16 pKa = 11.84 QLGVILPLVQAAFALLKK33 pKa = 10.3 KK34 pKa = 10.05 KK35 pKa = 10.14 PKK37 pKa = 9.88 RR38 pKa = 11.84 ALLLVGVALVSSRR51 pKa = 11.84 STKK54 pKa = 10.46 LGTVAQVLLKK64 pKa = 10.83 VFGGRR69 pKa = 11.84 GSKK72 pKa = 10.16 AA73 pKa = 2.96
Molecular weight: 7.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.361
IPC2_protein 10.862
IPC_protein 12.325
Toseland 12.53
ProMoST 12.983
Dawson 12.544
Bjellqvist 12.501
Wikipedia 12.983
Rodwell 12.647
Grimsley 12.574
Solomon 12.998
Lehninger 12.91
Nozaki 12.53
DTASelect 12.501
Thurlkill 12.53
EMBOSS 13.013
Sillero 12.53
Patrickios 12.369
IPC_peptide 12.998
IPC2_peptide 11.974
IPC2.peptide.svr19 8.904
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4246
0
4246
1220474
32
2489
287.4
31.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.028 ± 0.054
0.695 ± 0.011
8.085 ± 0.051
8.561 ± 0.055
3.467 ± 0.026
8.717 ± 0.039
1.937 ± 0.019
3.498 ± 0.029
1.89 ± 0.022
8.811 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.783 ± 0.014
2.416 ± 0.023
4.627 ± 0.023
2.242 ± 0.023
6.903 ± 0.045
5.767 ± 0.035
6.283 ± 0.032
9.382 ± 0.046
1.18 ± 0.016
2.724 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here