Thermoflexibacter ruber

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales;

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4522 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I2FW50|A0A1I2FW50_9BACT Uncharacterized protein OS=Thermoflexibacter ruber OX=1003 GN=SAMN04488541_1015110 PE=4 SV=1
AA1 pKa = 7.83INPNTQWYY9 pKa = 9.92LDD11 pKa = 3.68NDD13 pKa = 3.51NDD15 pKa = 4.14GFGAGAVAFTQCTKK29 pKa = 9.5PTGNYY34 pKa = 5.87VTNNTDD40 pKa = 3.87CNDD43 pKa = 3.23NDD45 pKa = 3.85TTLNPNTKK53 pKa = 8.86WYY55 pKa = 10.44RR56 pKa = 11.84DD57 pKa = 3.26ADD59 pKa = 3.58GDD61 pKa = 4.59GFGNPADD68 pKa = 4.76EE69 pKa = 4.85KK70 pKa = 9.53TQCLQPAGYY79 pKa = 9.81VRR81 pKa = 11.84NNTDD85 pKa = 3.22CDD87 pKa = 4.24DD88 pKa = 4.15NNVAINPTTVWYY100 pKa = 10.42RR101 pKa = 11.84DD102 pKa = 3.11ADD104 pKa = 3.58GDD106 pKa = 4.44GFGNPAITQVSCTQPTGFVLNNADD130 pKa = 4.77CNDD133 pKa = 3.37SDD135 pKa = 4.38ATLNPNTKK143 pKa = 8.87WYY145 pKa = 10.44RR146 pKa = 11.84DD147 pKa = 3.26ADD149 pKa = 3.58GDD151 pKa = 4.59GFGNPADD158 pKa = 4.76EE159 pKa = 4.85KK160 pKa = 9.53TQCLQPAGYY169 pKa = 9.81VRR171 pKa = 11.84NNTDD175 pKa = 3.43CNDD178 pKa = 3.3NNVAINPTTVWYY190 pKa = 10.39RR191 pKa = 11.84DD192 pKa = 3.17TDD194 pKa = 3.44GDD196 pKa = 4.27GFGNPAITQVSCTQPTGFVLNNADD220 pKa = 4.76CDD222 pKa = 3.94DD223 pKa = 4.08TKK225 pKa = 11.9ADD227 pKa = 3.94INPNTKK233 pKa = 8.89WYY235 pKa = 10.39RR236 pKa = 11.84DD237 pKa = 3.26ADD239 pKa = 3.58GDD241 pKa = 4.59GFGNPADD248 pKa = 4.76EE249 pKa = 4.85KK250 pKa = 9.53TQCLQPAGYY259 pKa = 9.81VRR261 pKa = 11.84NNTDD265 pKa = 3.82CNDD268 pKa = 3.13NNAAINPTTVWYY280 pKa = 10.42RR281 pKa = 11.84DD282 pKa = 3.11ADD284 pKa = 3.58GDD286 pKa = 4.44GFGNPAITQVSCTQPTGFVLNNADD310 pKa = 4.77CNDD313 pKa = 3.37SDD315 pKa = 4.38ATLNPNTKK323 pKa = 8.87WYY325 pKa = 10.44RR326 pKa = 11.84DD327 pKa = 3.26ADD329 pKa = 3.58GDD331 pKa = 4.59GFGNPADD338 pKa = 4.76EE339 pKa = 4.85KK340 pKa = 9.53TQCLQPAGYY349 pKa = 9.81VRR351 pKa = 11.84NNTDD355 pKa = 4.04CDD357 pKa = 3.69DD358 pKa = 3.92TKK360 pKa = 11.9ADD362 pKa = 4.3INPNTQWYY370 pKa = 9.88RR371 pKa = 11.84DD372 pKa = 3.41ADD374 pKa = 3.62GDD376 pKa = 4.59GFGNPADD383 pKa = 4.27VKK385 pKa = 8.09TQCLQPAGYY394 pKa = 9.81VRR396 pKa = 11.84NNTDD400 pKa = 3.43CNDD403 pKa = 3.3NNVAINPTTVWYY415 pKa = 10.42RR416 pKa = 11.84DD417 pKa = 3.11ADD419 pKa = 3.58GDD421 pKa = 4.27GFGNPTITQVSCTQPTGFVLSNADD445 pKa = 4.35CNDD448 pKa = 2.93SDD450 pKa = 4.38ATLNPNTKK458 pKa = 8.87WYY460 pKa = 10.44RR461 pKa = 11.84DD462 pKa = 3.26ADD464 pKa = 3.58GDD466 pKa = 4.59GFGNPADD473 pKa = 5.01AKK475 pKa = 7.57TQCLQPTGYY484 pKa = 10.02IRR486 pKa = 11.84DD487 pKa = 3.8NTDD490 pKa = 3.24CDD492 pKa = 3.7DD493 pKa = 3.87TKK495 pKa = 11.9ADD497 pKa = 4.3INPNTQWYY505 pKa = 9.88RR506 pKa = 11.84DD507 pKa = 3.41ADD509 pKa = 3.62GDD511 pKa = 4.59GFGNPADD518 pKa = 4.26VKK520 pKa = 10.36NQCLQPTGYY529 pKa = 10.47VRR531 pKa = 11.84DD532 pKa = 3.9NTDD535 pKa = 3.72CNDD538 pKa = 3.46SNASLNPNTKK548 pKa = 8.87WYY550 pKa = 10.55ADD552 pKa = 3.51NDD554 pKa = 3.86GDD556 pKa = 4.3GFGAATVAFTQCNKK570 pKa = 9.7PAGNYY575 pKa = 6.2VTNNTDD581 pKa = 4.0CDD583 pKa = 3.8DD584 pKa = 3.92TKK586 pKa = 11.9ADD588 pKa = 3.94INPNTKK594 pKa = 8.89WYY596 pKa = 10.39RR597 pKa = 11.84DD598 pKa = 3.26ADD600 pKa = 3.58GDD602 pKa = 4.74GFGNPANVKK611 pKa = 6.75TQCLQPAGYY620 pKa = 9.95IRR622 pKa = 11.84DD623 pKa = 3.78NTDD626 pKa = 3.55CNDD629 pKa = 3.35SDD631 pKa = 3.85ATLNPNTKK639 pKa = 8.89WYY641 pKa = 10.55ADD643 pKa = 3.51NDD645 pKa = 3.87GDD647 pKa = 4.48GFGAGPVVFVQCAKK661 pKa = 9.28PTGSYY666 pKa = 7.63VTNNGDD672 pKa = 4.62CDD674 pKa = 3.91DD675 pKa = 4.52TNPAIKK681 pKa = 8.61PTAVWYY687 pKa = 10.46RR688 pKa = 11.84DD689 pKa = 3.12ADD691 pKa = 3.56GDD693 pKa = 4.33GFGNPSISVVACSQPAGYY711 pKa = 10.95VMNNTDD717 pKa = 4.82CNDD720 pKa = 3.19NDD722 pKa = 4.34ASINPNTKK730 pKa = 8.92WYY732 pKa = 10.54ADD734 pKa = 3.58NDD736 pKa = 3.62NDD738 pKa = 4.35GFGAGAVAFTQCAKK752 pKa = 10.4PVGNFVTNNSDD763 pKa = 4.61CNDD766 pKa = 3.23NDD768 pKa = 3.38ATLNPNTKK776 pKa = 8.87WYY778 pKa = 10.44RR779 pKa = 11.84DD780 pKa = 3.26ADD782 pKa = 3.58GDD784 pKa = 4.59GFGNPADD791 pKa = 4.27VKK793 pKa = 8.09TQCLQPAGYY802 pKa = 9.95IRR804 pKa = 11.84DD805 pKa = 3.81NTDD808 pKa = 3.24CDD810 pKa = 3.7DD811 pKa = 3.87TKK813 pKa = 11.9ADD815 pKa = 3.94INPNTKK821 pKa = 8.89WYY823 pKa = 10.39RR824 pKa = 11.84DD825 pKa = 3.26ADD827 pKa = 3.58GDD829 pKa = 4.59GFGNPADD836 pKa = 4.06VRR838 pKa = 11.84TQCLQPVGYY847 pKa = 9.96VRR849 pKa = 11.84SNADD853 pKa = 3.9CNDD856 pKa = 3.06SDD858 pKa = 4.38ATLNPNTIWYY868 pKa = 9.54RR869 pKa = 11.84DD870 pKa = 3.26ADD872 pKa = 3.6GDD874 pKa = 4.28GFGNPSVTQKK884 pKa = 10.88SCTQPAGFVRR894 pKa = 11.84NNQDD898 pKa = 3.94CNDD901 pKa = 3.9SDD903 pKa = 5.16ASLNPNTKK911 pKa = 8.84WYY913 pKa = 10.44RR914 pKa = 11.84DD915 pKa = 3.26ADD917 pKa = 3.58GDD919 pKa = 4.59GFGNPADD926 pKa = 4.06VRR928 pKa = 11.84TQCLQPAGYY937 pKa = 9.81VRR939 pKa = 11.84NNTDD943 pKa = 3.72CDD945 pKa = 3.61DD946 pKa = 3.77TNRR949 pKa = 11.84NINPNTKK956 pKa = 9.24WYY958 pKa = 10.43LDD960 pKa = 3.43QDD962 pKa = 3.56GDD964 pKa = 4.86GFGDD968 pKa = 3.58PNNFIVSCTKK978 pKa = 10.27PVGNYY983 pKa = 8.99VLNGTDD989 pKa = 3.42NCPTLPNPNQVIPTWYY1005 pKa = 10.18LDD1007 pKa = 3.31QDD1009 pKa = 3.56RR1010 pKa = 11.84DD1011 pKa = 4.3GLGDD1015 pKa = 3.63PANAIQACSRR1025 pKa = 11.84PDD1027 pKa = 3.43GYY1029 pKa = 11.61VSNNTDD1035 pKa = 3.0NCPTIFNPDD1044 pKa = 2.59GTVPKK1049 pKa = 9.65WYY1051 pKa = 9.87RR1052 pKa = 11.84DD1053 pKa = 3.19ADD1055 pKa = 3.59GDD1057 pKa = 4.39GFGNPNVVVSACTAPIGYY1075 pKa = 9.52VPDD1078 pKa = 3.77NTDD1081 pKa = 4.68CDD1083 pKa = 4.48DD1084 pKa = 4.48SDD1086 pKa = 5.25AKK1088 pKa = 10.8INPNTIWYY1096 pKa = 9.69QDD1098 pKa = 3.14ADD1100 pKa = 3.5GDD1102 pKa = 4.49GFGNPSVTIKK1112 pKa = 10.8SCVKK1116 pKa = 7.8PTGYY1120 pKa = 10.57VSNKK1124 pKa = 9.64GDD1126 pKa = 4.36CNDD1129 pKa = 3.78NNPNVHH1135 pKa = 7.02PDD1137 pKa = 3.1ATEE1140 pKa = 3.94VPDD1143 pKa = 6.4GIDD1146 pKa = 3.41NNCNGQIDD1154 pKa = 4.57EE1155 pKa = 4.62SSLVYY1160 pKa = 9.6QVYY1163 pKa = 10.15PNPSVDD1169 pKa = 2.81GRR1171 pKa = 11.84FYY1173 pKa = 11.47LDD1175 pKa = 3.41YY1176 pKa = 11.06QNPADD1181 pKa = 3.8SQIQVVVVSITGTIVYY1197 pKa = 9.03QRR1199 pKa = 11.84NVFLTNGKK1207 pKa = 8.87VQGFFIDD1214 pKa = 3.59MTNQASGSYY1223 pKa = 8.71IVRR1226 pKa = 11.84IISSTTNVSVKK1237 pKa = 10.33VIKK1240 pKa = 10.59LL1241 pKa = 3.43

Molecular weight: 134.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I2HHM9|A0A1I2HHM9_9BACT NADPH:quinone reductase OS=Thermoflexibacter ruber OX=1003 GN=SAMN04488541_102353 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.21KK3 pKa = 9.99LHH5 pKa = 6.42LLFVKK10 pKa = 10.63ALLLLFLSLVFGTHH24 pKa = 6.42HH25 pKa = 6.21STAQVIIIQPRR36 pKa = 11.84MPQVIVVPPRR46 pKa = 11.84VIITTPRR53 pKa = 11.84GFRR56 pKa = 11.84NPRR59 pKa = 11.84HH60 pKa = 5.03FWGGRR65 pKa = 11.84KK66 pKa = 7.72FQRR69 pKa = 11.84KK70 pKa = 8.86LYY72 pKa = 9.85RR73 pKa = 11.84HH74 pKa = 6.54RR75 pKa = 11.84GWGWRR80 pKa = 11.84RR81 pKa = 11.84WW82 pKa = 3.44

Molecular weight: 9.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4522

0

4522

1625937

25

5668

359.6

40.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.773 ± 0.031

0.832 ± 0.012

4.822 ± 0.025

6.644 ± 0.051

5.317 ± 0.028

6.229 ± 0.043

1.776 ± 0.017

7.738 ± 0.034

7.595 ± 0.055

9.667 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.13 ± 0.019

5.872 ± 0.049

3.54 ± 0.024

4.293 ± 0.026

3.83 ± 0.025

6.051 ± 0.032

5.537 ± 0.059

6.015 ± 0.031

1.169 ± 0.014

4.171 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski