Amino acid dipepetide frequency for Thermoflexibacter ruber

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.557AlaAla: 3.557 ± 0.063	0.73AlaCys: 0.73 ± 0.021	3.525AlaAsp: 3.525 ± 0.047	4.572AlaGlu: 4.572 ± 0.06	3.434AlaPhe: 3.434 ± 0.05	4.604AlaGly: 4.604 ± 0.08	1.195AlaHis: 1.195 ± 0.031	5.116AlaIle: 5.116 ± 0.059	5.214AlaLys: 5.214 ± 0.061	6.641AlaLeu: 6.641 ± 0.068	1.627AlaMet: 1.627 ± 0.035	4.195AlaAsn: 4.195 ± 0.077	2.023AlaPro: 2.023 ± 0.037	3.241AlaGln: 3.241 ± 0.045	2.279AlaArg: 2.279 ± 0.039	4.178AlaSer: 4.178 ± 0.054	3.808AlaThr: 3.808 ± 0.084	4.145AlaVal: 4.145 ± 0.056	0.842AlaTrp: 0.842 ± 0.026	2.806AlaTyr: 2.806 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	0.507CysAla: 0.507 ± 0.021	0.121CysCys: 0.121 ± 0.01	0.409CysAsp: 0.409 ± 0.014	0.528CysGlu: 0.528 ± 0.018	0.507CysPhe: 0.507 ± 0.018	0.643CysGly: 0.643 ± 0.022	0.202CysHis: 0.202 ± 0.012	0.653CysIle: 0.653 ± 0.02	0.518CysLys: 0.518 ± 0.019	0.841CysLeu: 0.841 ± 0.028	0.156CysMet: 0.156 ± 0.01	0.404CysAsn: 0.404 ± 0.019	0.343CysPro: 0.343 ± 0.019	0.338CysGln: 0.338 ± 0.013	0.275CysArg: 0.275 ± 0.013	0.514CysSer: 0.514 ± 0.02	0.461CysThr: 0.461 ± 0.02	0.475CysVal: 0.475 ± 0.016	0.09CysTrp: 0.09 ± 0.007	0.334CysTyr: 0.334 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	2.976AspAla: 2.976 ± 0.048	0.436AspCys: 0.436 ± 0.022	2.025AspAsp: 2.025 ± 0.04	3.146AspGlu: 3.146 ± 0.047	3.53AspPhe: 3.53 ± 0.051	3.117AspGly: 3.117 ± 0.063	0.552AspHis: 0.552 ± 0.018	3.903AspIle: 3.903 ± 0.053	4.14AspLys: 4.14 ± 0.065	5.031AspLeu: 5.031 ± 0.065	1.046AspMet: 1.046 ± 0.025	2.792AspAsn: 2.792 ± 0.047	1.417AspPro: 1.417 ± 0.029	1.126AspGln: 1.126 ± 0.026	1.943AspArg: 1.943 ± 0.037	2.785AspSer: 2.785 ± 0.047	2.481AspThr: 2.481 ± 0.045	2.664AspVal: 2.664 ± 0.048	0.724AspTrp: 0.724 ± 0.021	2.386AspTyr: 2.386 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	4.648GluAla: 4.648 ± 0.066	0.324GluCys: 0.324 ± 0.016	2.827GluAsp: 2.827 ± 0.053	5.123GluGlu: 5.123 ± 0.072	2.531GluPhe: 2.531 ± 0.04	3.857GluGly: 3.857 ± 0.052	1.055GluHis: 1.055 ± 0.028	5.922GluIle: 5.922 ± 0.069	7.044GluLys: 7.044 ± 0.086	5.83GluLeu: 5.83 ± 0.063	1.681GluMet: 1.681 ± 0.036	4.521GluAsn: 4.521 ± 0.064	1.55GluPro: 1.55 ± 0.037	3.001GluGln: 3.001 ± 0.049	2.75GluArg: 2.75 ± 0.051	3.162GluSer: 3.162 ± 0.043	2.865GluThr: 2.865 ± 0.042	4.621GluVal: 4.621 ± 0.057	0.732GluTrp: 0.732 ± 0.024	2.394GluTyr: 2.394 ± 0.046	0.0GluXaa: 0.0 ± 0.0
Phe	3.809PheAla: 3.809 ± 0.052	0.56PheCys: 0.56 ± 0.021	2.965PheAsp: 2.965 ± 0.051	3.169PheGlu: 3.169 ± 0.052	3.172PhePhe: 3.172 ± 0.06	3.251PheGly: 3.251 ± 0.053	0.887PheHis: 0.887 ± 0.022	3.885PheIle: 3.885 ± 0.054	3.279PheLys: 3.279 ± 0.048	5.208PheLeu: 5.208 ± 0.066	1.071PheMet: 1.071 ± 0.024	2.702PheAsn: 2.702 ± 0.053	1.949PhePro: 1.949 ± 0.036	1.969PheGln: 1.969 ± 0.032	1.94PheArg: 1.94 ± 0.037	4.016PheSer: 4.016 ± 0.055	3.171PheThr: 3.171 ± 0.055	3.098PheVal: 3.098 ± 0.05	0.651PheTrp: 0.651 ± 0.022	2.416PheTyr: 2.416 ± 0.048	0.0PheXaa: 0.0 ± 0.0
Gly	4.069GlyAla: 4.069 ± 0.067	0.56GlyCys: 0.56 ± 0.022	2.81GlyAsp: 2.81 ± 0.05	3.706GlyGlu: 3.706 ± 0.055	3.398GlyPhe: 3.398 ± 0.05	4.655GlyGly: 4.655 ± 0.083	1.008GlyHis: 1.008 ± 0.029	5.118GlyIle: 5.118 ± 0.067	5.441GlyLys: 5.441 ± 0.067	5.747GlyLeu: 5.747 ± 0.066	1.57GlyMet: 1.57 ± 0.031	3.701GlyAsn: 3.701 ± 0.08	0.723GlyPro: 0.723 ± 0.025	2.177GlyGln: 2.177 ± 0.037	2.418GlyArg: 2.418 ± 0.047	3.672GlySer: 3.672 ± 0.066	4.043GlyThr: 4.043 ± 0.112	4.084GlyVal: 4.084 ± 0.058	0.733GlyTrp: 0.733 ± 0.025	2.656GlyTyr: 2.656 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	1.057HisAla: 1.057 ± 0.027	0.188HisCys: 0.188 ± 0.011	0.725HisAsp: 0.725 ± 0.025	0.984HisGlu: 0.984 ± 0.024	1.193HisPhe: 1.193 ± 0.033	0.998HisGly: 0.998 ± 0.025	0.518HisHis: 0.518 ± 0.019	1.527HisIle: 1.527 ± 0.038	1.039HisLys: 1.039 ± 0.029	2.097HisLeu: 2.097 ± 0.041	0.151HisMet: 0.151 ± 0.009	0.809HisAsn: 0.809 ± 0.023	0.867HisPro: 0.867 ± 0.024	0.95HisGln: 0.95 ± 0.027	0.744HisArg: 0.744 ± 0.022	1.01HisSer: 1.01 ± 0.028	0.991HisThr: 0.991 ± 0.026	0.766HisVal: 0.766 ± 0.023	0.274HisTrp: 0.274 ± 0.014	0.868HisTyr: 0.868 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.894IleAla: 5.894 ± 0.068	0.724IleCys: 0.724 ± 0.024	4.548IleAsp: 4.548 ± 0.057	5.262IleGlu: 5.262 ± 0.07	4.221IlePhe: 4.221 ± 0.056	5.124IleGly: 5.124 ± 0.073	1.392IleHis: 1.392 ± 0.034	5.278IleIle: 5.278 ± 0.068	5.227IleLys: 5.227 ± 0.067	7.02IleLeu: 7.02 ± 0.072	1.237IleMet: 1.237 ± 0.027	4.204IleAsn: 4.204 ± 0.06	3.046IlePro: 3.046 ± 0.047	2.897IleGln: 2.897 ± 0.043	3.073IleArg: 3.073 ± 0.044	5.32IleSer: 5.32 ± 0.062	4.482IleThr: 4.482 ± 0.072	4.552IleVal: 4.552 ± 0.059	0.766IleTrp: 0.766 ± 0.025	3.107IleTyr: 3.107 ± 0.047	0.0IleXaa: 0.0 ± 0.0
Lys	5.089LysAla: 5.089 ± 0.064	0.343LysCys: 0.343 ± 0.015	4.116LysAsp: 4.116 ± 0.065	6.46LysGlu: 6.46 ± 0.075	2.872LysPhe: 2.872 ± 0.046	4.476LysGly: 4.476 ± 0.067	1.268LysHis: 1.268 ± 0.028	6.349LysIle: 6.349 ± 0.061	6.429LysLys: 6.429 ± 0.085	6.348LysLeu: 6.348 ± 0.071	1.938LysMet: 1.938 ± 0.042	5.146LysAsn: 5.146 ± 0.064	2.56LysPro: 2.56 ± 0.043	3.483LysGln: 3.483 ± 0.064	2.687LysArg: 2.687 ± 0.046	3.807LysSer: 3.807 ± 0.051	3.85LysThr: 3.85 ± 0.049	4.83LysVal: 4.83 ± 0.061	0.745LysTrp: 0.745 ± 0.025	3.151LysTyr: 3.151 ± 0.049	0.0LysXaa: 0.0 ± 0.0
Leu	7.251LeuAla: 7.251 ± 0.069	0.878LeuCys: 0.878 ± 0.027	4.563LeuAsp: 4.563 ± 0.062	5.956LeuGlu: 5.956 ± 0.073	5.1LeuPhe: 5.1 ± 0.067	5.753LeuGly: 5.753 ± 0.063	1.827LeuHis: 1.827 ± 0.04	6.956LeuIle: 6.956 ± 0.08	7.125LeuLys: 7.125 ± 0.077	9.865LeuLeu: 9.865 ± 0.099	2.002LeuMet: 2.002 ± 0.044	5.06LeuAsn: 5.06 ± 0.06	4.068LeuPro: 4.068 ± 0.054	4.156LeuGln: 4.156 ± 0.061	3.854LeuArg: 3.854 ± 0.051	6.829LeuSer: 6.829 ± 0.084	5.327LeuThr: 5.327 ± 0.066	5.575LeuVal: 5.575 ± 0.065	0.995LeuTrp: 0.995 ± 0.028	3.536LeuTyr: 3.536 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	1.34MetAla: 1.34 ± 0.031	0.135MetCys: 0.135 ± 0.009	0.85MetAsp: 0.85 ± 0.027	1.325MetGlu: 1.325 ± 0.031	0.773MetPhe: 0.773 ± 0.025	1.318MetGly: 1.318 ± 0.034	0.378MetHis: 0.378 ± 0.015	1.469MetIle: 1.469 ± 0.031	2.11MetLys: 2.11 ± 0.037	2.213MetLeu: 2.213 ± 0.046	0.531MetMet: 0.531 ± 0.019	1.258MetAsn: 1.258 ± 0.028	0.983MetPro: 0.983 ± 0.026	1.1MetGln: 1.1 ± 0.025	1.022MetArg: 1.022 ± 0.021	1.365MetSer: 1.365 ± 0.033	1.143MetThr: 1.143 ± 0.029	1.131MetVal: 1.131 ± 0.027	0.173MetTrp: 0.173 ± 0.01	0.683MetTyr: 0.683 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	3.942AsnAla: 3.942 ± 0.065	0.463AsnCys: 0.463 ± 0.018	2.545AsnAsp: 2.545 ± 0.052	3.61AsnGlu: 3.61 ± 0.043	3.19AsnPhe: 3.19 ± 0.053	3.69AsnGly: 3.69 ± 0.07	1.052AsnHis: 1.052 ± 0.026	4.567AsnIle: 4.567 ± 0.061	3.745AsnLys: 3.745 ± 0.046	5.893AsnLeu: 5.893 ± 0.071	1.153AsnMet: 1.153 ± 0.029	3.441AsnAsn: 3.441 ± 0.077	3.037AsnPro: 3.037 ± 0.055	2.919AsnGln: 2.919 ± 0.048	2.4AsnArg: 2.4 ± 0.041	3.167AsnSer: 3.167 ± 0.06	3.367AsnThr: 3.367 ± 0.08	3.135AsnVal: 3.135 ± 0.059	0.742AsnTrp: 0.742 ± 0.025	2.661AsnTyr: 2.661 ± 0.041	0.0AsnXaa: 0.0 ± 0.0
Pro	2.475ProAla: 2.475 ± 0.05	0.244ProCys: 0.244 ± 0.012	2.038ProAsp: 2.038 ± 0.036	2.541ProGlu: 2.541 ± 0.039	1.865ProPhe: 1.865 ± 0.035	1.018ProGly: 1.018 ± 0.037	0.74ProHis: 0.74 ± 0.024	2.765ProIle: 2.765 ± 0.041	2.337ProLys: 2.337 ± 0.043	3.302ProLeu: 3.302 ± 0.046	0.765ProMet: 0.765 ± 0.022	2.591ProAsn: 2.591 ± 0.055	1.324ProPro: 1.324 ± 0.039	1.713ProGln: 1.713 ± 0.034	1.053ProArg: 1.053 ± 0.026	2.255ProSer: 2.255 ± 0.045	2.464ProThr: 2.464 ± 0.057	2.1ProVal: 2.1 ± 0.041	0.315ProTrp: 0.315 ± 0.016	1.493ProTyr: 1.493 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	3.042GlnAla: 3.042 ± 0.04	0.249GlnCys: 0.249 ± 0.014	1.926GlnAsp: 1.926 ± 0.033	3.15GlnGlu: 3.15 ± 0.044	1.862GlnPhe: 1.862 ± 0.035	2.455GlnGly: 2.455 ± 0.041	0.739GlnHis: 0.739 ± 0.02	3.682GlnIle: 3.682 ± 0.047	3.64GlnLys: 3.64 ± 0.051	3.738GlnLeu: 3.738 ± 0.053	0.975GlnMet: 0.975 ± 0.025	2.677GlnAsn: 2.677 ± 0.047	1.249GlnPro: 1.249 ± 0.031	2.018GlnGln: 2.018 ± 0.037	1.78GlnArg: 1.78 ± 0.037	2.482GlnSer: 2.482 ± 0.041	2.449GlnThr: 2.449 ± 0.044	2.773GlnVal: 2.773 ± 0.047	0.48GlnTrp: 0.48 ± 0.02	1.562GlnTyr: 1.562 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	2.267ArgAla: 2.267 ± 0.039	0.245ArgCys: 0.245 ± 0.012	1.658ArgAsp: 1.658 ± 0.037	2.541ArgGlu: 2.541 ± 0.044	2.215ArgPhe: 2.215 ± 0.045	2.13ArgGly: 2.13 ± 0.042	0.631ArgHis: 0.631 ± 0.022	3.13ArgIle: 3.13 ± 0.051	3.113ArgLys: 3.113 ± 0.047	3.886ArgLeu: 3.886 ± 0.051	0.937ArgMet: 0.937 ± 0.024	2.44ArgAsn: 2.44 ± 0.047	1.234ArgPro: 1.234 ± 0.031	1.757ArgGln: 1.757 ± 0.036	1.63ArgArg: 1.63 ± 0.032	1.861ArgSer: 1.861 ± 0.032	2.053ArgThr: 2.053 ± 0.036	2.231ArgVal: 2.231 ± 0.038	0.509ArgTrp: 0.509 ± 0.022	1.833ArgTyr: 1.833 ± 0.042	0.001ArgXaa: 0.001 ± 0.001
Ser	3.916SerAla: 3.916 ± 0.057	0.573SerCys: 0.573 ± 0.02	2.607SerAsp: 2.607 ± 0.035	3.722SerGlu: 3.722 ± 0.054	3.646SerPhe: 3.646 ± 0.049	4.037SerGly: 4.037 ± 0.073	1.05SerHis: 1.05 ± 0.027	4.883SerIle: 4.883 ± 0.07	4.283SerLys: 4.283 ± 0.059	6.374SerLeu: 6.374 ± 0.078	1.301SerMet: 1.301 ± 0.03	3.289SerAsn: 3.289 ± 0.06	2.618SerPro: 2.618 ± 0.056	2.593SerGln: 2.593 ± 0.039	2.046SerArg: 2.046 ± 0.041	3.567SerSer: 3.567 ± 0.062	3.178SerThr: 3.178 ± 0.057	3.524SerVal: 3.524 ± 0.05	0.737SerTrp: 0.737 ± 0.022	2.562SerTyr: 2.562 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	3.873ThrAla: 3.873 ± 0.07	0.434ThrCys: 0.434 ± 0.015	2.881ThrAsp: 2.881 ± 0.043	3.295ThrGlu: 3.295 ± 0.044	3.167ThrPhe: 3.167 ± 0.051	3.861ThrGly: 3.861 ± 0.085	1.101ThrHis: 1.101 ± 0.026	4.043ThrIle: 4.043 ± 0.063	3.328ThrLys: 3.328 ± 0.053	5.462ThrLeu: 5.462 ± 0.059	0.924ThrMet: 0.924 ± 0.026	3.295ThrAsn: 3.295 ± 0.083	2.495ThrPro: 2.495 ± 0.053	2.283ThrGln: 2.283 ± 0.043	1.536ThrArg: 1.536 ± 0.032	3.4ThrSer: 3.4 ± 0.067	3.327ThrThr: 3.327 ± 0.084	3.471ThrVal: 3.471 ± 0.089	0.766ThrTrp: 0.766 ± 0.031	2.431ThrTyr: 2.431 ± 0.044	0.0ThrXaa: 0.0 ± 0.0
Val	4.382ValAla: 4.382 ± 0.063	0.605ValCys: 0.605 ± 0.023	2.465ValAsp: 2.465 ± 0.041	3.877ValGlu: 3.877 ± 0.052	3.307ValPhe: 3.307 ± 0.05	4.103ValGly: 4.103 ± 0.059	0.993ValHis: 0.993 ± 0.025	4.605ValIle: 4.605 ± 0.058	4.358ValLys: 4.358 ± 0.053	5.636ValLeu: 5.636 ± 0.07	1.282ValMet: 1.282 ± 0.028	3.358ValAsn: 3.358 ± 0.053	2.272ValPro: 2.272 ± 0.031	2.099ValGln: 2.099 ± 0.036	2.475ValArg: 2.475 ± 0.042	4.171ValSer: 4.171 ± 0.059	3.054ValThr: 3.054 ± 0.085	4.056ValVal: 4.056 ± 0.056	0.679ValTrp: 0.679 ± 0.02	2.378ValTyr: 2.378 ± 0.046	0.0ValXaa: 0.0 ± 0.0
Trp	0.779TrpAla: 0.779 ± 0.022	0.111TrpCys: 0.111 ± 0.007	0.534TrpAsp: 0.534 ± 0.019	0.826TrpGlu: 0.826 ± 0.021	0.571TrpPhe: 0.571 ± 0.017	0.766TrpGly: 0.766 ± 0.024	0.25TrpHis: 0.25 ± 0.015	0.83TrpIle: 0.83 ± 0.023	0.877TrpLys: 0.877 ± 0.024	1.21TrpLeu: 1.21 ± 0.03	0.202TrpMet: 0.202 ± 0.012	0.687TrpAsn: 0.687 ± 0.028	0.079TrpPro: 0.079 ± 0.007	0.729TrpGln: 0.729 ± 0.019	0.518TrpArg: 0.518 ± 0.019	0.596TrpSer: 0.596 ± 0.022	0.677TrpThr: 0.677 ± 0.036	0.785TrpVal: 0.785 ± 0.021	0.197TrpTrp: 0.197 ± 0.013	0.464TrpTyr: 0.464 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.862TyrAla: 2.862 ± 0.047	0.396TyrCys: 0.396 ± 0.016	2.203TyrAsp: 2.203 ± 0.038	2.649TyrGlu: 2.649 ± 0.038	2.616TyrPhe: 2.616 ± 0.041	2.433TyrGly: 2.433 ± 0.046	0.916TyrHis: 0.916 ± 0.027	2.694TyrIle: 2.694 ± 0.045	2.701TyrLys: 2.701 ± 0.046	4.333TyrLeu: 4.333 ± 0.061	0.75TyrMet: 0.75 ± 0.024	2.149TyrAsn: 2.149 ± 0.04	1.56TyrPro: 1.56 ± 0.034	2.378TyrGln: 2.378 ± 0.041	1.876TyrArg: 1.876 ± 0.041	2.351TyrSer: 2.351 ± 0.041	2.181TyrThr: 2.181 ± 0.039	2.137TyrVal: 2.137 ± 0.035	0.535TyrTrp: 0.535 ± 0.019	1.993TyrTyr: 1.993 ± 0.038	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.002XaaXaa: 0.002 ± 0.002

Statistics based on 4522 proteins (1625937 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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