Penaeid shrimp infectious myonecrosis virus
Average proteome isoelectric point is 7.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q24M82|Q24M82_9VIRU Structural protein OS=Penaeid shrimp infectious myonecrosis virus OX=282786 PE=4 SV=1
MM1 pKa = 7.21 HH2 pKa = 7.08 VEE4 pKa = 3.79 NGNIVSMEE12 pKa = 4.06 NQSEE16 pKa = 4.08 IDD18 pKa = 3.63 SQTKK22 pKa = 9.54 FFSLLEE28 pKa = 4.79 DD29 pKa = 4.34 DD30 pKa = 4.47 NKK32 pKa = 11.16 LPIVDD37 pKa = 4.26 EE38 pKa = 4.4 LRR40 pKa = 11.84 VLADD44 pKa = 3.23 MTAQRR49 pKa = 11.84 SNVNTAGNHH58 pKa = 5.48 LRR60 pKa = 11.84 DD61 pKa = 3.88 NDD63 pKa = 4.53 SIRR66 pKa = 11.84 ADD68 pKa = 2.92 AVLANNTVRR77 pKa = 11.84 NNCQIPIPVTTLIPRR92 pKa = 11.84 QIRR95 pKa = 11.84 GLNGVLVNQQLRR107 pKa = 11.84 LQGIEE112 pKa = 3.74 THH114 pKa = 6.1 ITDD117 pKa = 3.87 SYY119 pKa = 10.59 ISKK122 pKa = 10.61 AEE124 pKa = 3.81 PSDD127 pKa = 3.73 YY128 pKa = 11.0 SKK130 pKa = 11.03 QLSEE134 pKa = 4.18 MVNAQKK140 pKa = 10.04 TSTWRR145 pKa = 11.84 ANNIASQGWDD155 pKa = 2.97 MFDD158 pKa = 3.0 TVQLNTNISQKK169 pKa = 10.59 DD170 pKa = 3.63 LSMDD174 pKa = 3.54 TALTKK179 pKa = 10.93 LMLLYY184 pKa = 10.68 QLTTQNLPATQLPSSIYY201 pKa = 10.05 SAFDD205 pKa = 3.06 SRR207 pKa = 11.84 TQPTLQDD214 pKa = 4.56 GIWGINNGVNIFGEE228 pKa = 4.31 QCGGLAAPVFPFSGGTGEE246 pKa = 4.19 ITFHH250 pKa = 6.41 LTLQSVPQEE259 pKa = 4.18 FQEE262 pKa = 4.34 SAIFVPATALQAAKK276 pKa = 10.32 EE277 pKa = 4.22 GARR280 pKa = 11.84 TLAMYY285 pKa = 11.21 VLMFAEE291 pKa = 5.25 WPFGMYY297 pKa = 9.06 TKK299 pKa = 10.27 TKK301 pKa = 8.87 QTTDD305 pKa = 2.97 NAGNNQSDD313 pKa = 3.65 QIFIHH318 pKa = 6.17 SEE320 pKa = 4.12 STVHH324 pKa = 7.25 IPGQKK329 pKa = 9.48 QMHH332 pKa = 6.05 IVLPRR337 pKa = 11.84 KK338 pKa = 10.06 VNMVNPTTIAEE349 pKa = 3.98 ANARR353 pKa = 11.84 VVIQPTYY360 pKa = 8.63 GTVAAGAGVANGNINVAAVGVALPTVNLTDD390 pKa = 4.23 YY391 pKa = 10.33 LVSWATDD398 pKa = 3.58 FTLGDD403 pKa = 3.2 IKK405 pKa = 11.06 QLVEE409 pKa = 4.12 RR410 pKa = 11.84 MKK412 pKa = 8.68 TTLPISRR419 pKa = 11.84 DD420 pKa = 3.09 LMAARR425 pKa = 11.84 QNAMLLSTLFPPLIQSNVASDD446 pKa = 3.59 TKK448 pKa = 10.45 EE449 pKa = 4.03 VPGTAGAYY457 pKa = 6.6 TACLANLGIPEE468 pKa = 4.46 TLTVNWGVDD477 pKa = 3.28 INVQPLYY484 pKa = 10.78 QLLEE488 pKa = 4.12 TDD490 pKa = 3.11 ITAHH494 pKa = 5.74 NRR496 pKa = 11.84 YY497 pKa = 9.11 VLNLFKK503 pKa = 10.76 RR504 pKa = 11.84 EE505 pKa = 3.88 EE506 pKa = 4.38 VVAGAYY512 pKa = 8.82 EE513 pKa = 4.39 FGWLGHH519 pKa = 5.57 MASYY523 pKa = 10.81 MMGLLLTMNISSVFNVWYY541 pKa = 7.35 STRR544 pKa = 11.84 RR545 pKa = 11.84 ISTKK549 pKa = 10.58 AWDD552 pKa = 3.45 TAYY555 pKa = 10.79 DD556 pKa = 3.98 SNIQAYY562 pKa = 10.05 QDD564 pKa = 2.88 MHH566 pKa = 7.63 YY567 pKa = 11.24 QMFSWSSMQGSIAPAMVDD585 pKa = 3.58 EE586 pKa = 4.97 ILHH589 pKa = 5.58 NLCGQMFGFSLPLRR603 pKa = 11.84 QVLFNALPITFSSFGSWMLPRR624 pKa = 11.84 VSDD627 pKa = 3.79 GFQTVRR633 pKa = 11.84 YY634 pKa = 8.84 YY635 pKa = 11.11 DD636 pKa = 3.3 VGPPVINAKK645 pKa = 9.98 RR646 pKa = 11.84 DD647 pKa = 3.57 GEE649 pKa = 4.71 VPVSMIDD656 pKa = 2.56 AWTYY660 pKa = 11.24 KK661 pKa = 9.42 FTEE664 pKa = 4.27 KK665 pKa = 10.51 LPKK668 pKa = 10.28 SFLPWPMPEE677 pKa = 4.64 GKK679 pKa = 9.76 DD680 pKa = 3.19 STMGYY685 pKa = 10.46 DD686 pKa = 3.71 PEE688 pKa = 5.43 KK689 pKa = 10.85 EE690 pKa = 3.68 PALIDD695 pKa = 3.61 NSNEE699 pKa = 3.73 TGNVFRR705 pKa = 11.84 PFMARR710 pKa = 11.84 NGNNSNYY717 pKa = 10.19 LPTNYY722 pKa = 10.08 TIDD725 pKa = 3.52 VSQNGHH731 pKa = 6.86 DD732 pKa = 3.73 EE733 pKa = 4.12 SCINVDD739 pKa = 4.55 LFNNVAGVTLTNYY752 pKa = 10.74 DD753 pKa = 3.48 GTATNADD760 pKa = 3.75 VVPTGSYY767 pKa = 9.59 IKK769 pKa = 10.27 QRR771 pKa = 11.84 AMPINANAVRR781 pKa = 11.84 PTEE784 pKa = 4.02 TLDD787 pKa = 3.68 AANHH791 pKa = 5.42 TKK793 pKa = 10.33 PFAIEE798 pKa = 3.96 GGRR801 pKa = 11.84 LVYY804 pKa = 10.78 LGGTIANTTNVVNAMQRR821 pKa = 11.84 KK822 pKa = 8.09 QRR824 pKa = 11.84 LSKK827 pKa = 10.34 PAFKK831 pKa = 9.46 WAHH834 pKa = 5.1 AQRR837 pKa = 11.84 QRR839 pKa = 11.84 VYY841 pKa = 10.69 DD842 pKa = 3.61 SSRR845 pKa = 11.84 PGMDD849 pKa = 5.52 AITKK853 pKa = 9.57 LCARR857 pKa = 11.84 KK858 pKa = 9.99 SGFMNARR865 pKa = 11.84 STAMMAPKK873 pKa = 9.55 TGLSAVIDD881 pKa = 3.82 QAPNTSQDD889 pKa = 4.11 LIEE892 pKa = 4.66 QPSQQEE898 pKa = 4.18 VMDD901 pKa = 3.96 MQATATVV908 pKa = 3.35
Molecular weight: 100.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.303
IPC2_protein 5.308
IPC_protein 5.308
Toseland 5.461
ProMoST 5.537
Dawson 5.41
Bjellqvist 5.499
Wikipedia 5.347
Rodwell 5.347
Grimsley 5.474
Solomon 5.41
Lehninger 5.385
Nozaki 5.588
DTASelect 5.779
Thurlkill 5.55
EMBOSS 5.525
Sillero 5.664
Patrickios 4.062
IPC_peptide 5.423
IPC2_peptide 5.664
IPC2.peptide.svr19 5.652
Protein with the highest isoelectric point:
>tr|Q24M82|Q24M82_9VIRU Structural protein OS=Penaeid shrimp infectious myonecrosis virus OX=282786 PE=4 SV=1
MM1 pKa = 8.18 DD2 pKa = 5.69 EE3 pKa = 4.04 IRR5 pKa = 11.84 NYY7 pKa = 8.96 THH9 pKa = 7.32 LDD11 pKa = 3.5 YY12 pKa = 11.25 IFVQNICIYY21 pKa = 9.1 MLVFGIDD28 pKa = 3.4 TVKK31 pKa = 10.48 HH32 pKa = 5.3 FRR34 pKa = 11.84 QIGLFNEE41 pKa = 3.88 RR42 pKa = 11.84 NEE44 pKa = 4.42 FIEE47 pKa = 4.16 IAKK50 pKa = 9.96 QLSTKK55 pKa = 9.84 GKK57 pKa = 10.13 RR58 pKa = 11.84 FVDD61 pKa = 3.92 DD62 pKa = 3.63 VDD64 pKa = 3.55 NMKK67 pKa = 10.77 QKK69 pKa = 9.97 VCEE72 pKa = 3.56 IATIVGYY79 pKa = 9.39 MDD81 pKa = 4.86 PNVDD85 pKa = 4.06 KK86 pKa = 10.56 IDD88 pKa = 3.46 VMEE91 pKa = 4.54 EE92 pKa = 4.15 VNSLAAEE99 pKa = 4.38 GNEE102 pKa = 4.27 HH103 pKa = 7.47 GIDD106 pKa = 3.33 RR107 pKa = 11.84 DD108 pKa = 3.28 NWNDD112 pKa = 4.33 LFTKK116 pKa = 8.76 TCKK119 pKa = 10.25 EE120 pKa = 3.6 VMTWYY125 pKa = 10.14 KK126 pKa = 9.88 GHH128 pKa = 6.32 EE129 pKa = 4.66 FISFDD134 pKa = 3.95 DD135 pKa = 4.37 YY136 pKa = 11.35 IKK138 pKa = 11.04 EE139 pKa = 4.23 GMWLTSGSSSIGKK152 pKa = 7.43 VHH154 pKa = 5.05 WTKK157 pKa = 11.03 DD158 pKa = 3.32 GEE160 pKa = 4.31 NGKK163 pKa = 10.2 FKK165 pKa = 10.87 ARR167 pKa = 11.84 KK168 pKa = 9.39 NMLLQIYY175 pKa = 7.67 TPQEE179 pKa = 3.67 LANIVYY185 pKa = 9.94 AWDD188 pKa = 3.91 GKK190 pKa = 9.1 LHH192 pKa = 5.97 SRR194 pKa = 11.84 VFIKK198 pKa = 11.08 NEE200 pKa = 3.64 MSKK203 pKa = 10.74 LRR205 pKa = 11.84 LAVASNIEE213 pKa = 4.12 AYY215 pKa = 9.57 IHH217 pKa = 5.95 EE218 pKa = 4.82 SYY220 pKa = 10.8 MLFLYY225 pKa = 10.2 GHH227 pKa = 6.35 GFKK230 pKa = 10.44 EE231 pKa = 4.4 YY232 pKa = 10.66 FGVTLDD238 pKa = 3.99 EE239 pKa = 5.7 KK240 pKa = 10.82 PDD242 pKa = 3.51 QQHH245 pKa = 4.28 QRR247 pKa = 11.84 EE248 pKa = 4.02 IEE250 pKa = 4.18 MIEE253 pKa = 4.1 KK254 pKa = 10.09 LQAGYY259 pKa = 10.45 FGLPFDD265 pKa = 4.37 YY266 pKa = 10.95 ASFDD270 pKa = 3.59 HH271 pKa = 6.68 QPTTFEE277 pKa = 4.0 VKK279 pKa = 9.62 TMVRR283 pKa = 11.84 RR284 pKa = 11.84 VGEE287 pKa = 4.07 IVVSQVPKK295 pKa = 9.84 NYY297 pKa = 10.37 YY298 pKa = 9.77 YY299 pKa = 9.29 QTQLLVNKK307 pKa = 9.81 IVNAYY312 pKa = 9.35 DD313 pKa = 3.3 KK314 pKa = 10.96 SYY316 pKa = 11.44 LSGNIKK322 pKa = 8.18 NTKK325 pKa = 9.62 FEE327 pKa = 4.46 NIKK330 pKa = 10.73 VKK332 pKa = 10.73 GGVPSGVRR340 pKa = 11.84 ITSLLGNMWNAIITKK355 pKa = 9.84 IAINNVIGIIGYY367 pKa = 9.77 DD368 pKa = 3.91 PISQISLRR376 pKa = 11.84 GDD378 pKa = 3.3 DD379 pKa = 3.83 VAILSKK385 pKa = 11.1 DD386 pKa = 3.24 PAALYY391 pKa = 10.44 LLRR394 pKa = 11.84 LSYY397 pKa = 10.88 AAINAIGKK405 pKa = 9.48 DD406 pKa = 3.52 SKK408 pKa = 11.22 LGISPKK414 pKa = 9.65 VCEE417 pKa = 4.14 FLRR420 pKa = 11.84 NEE422 pKa = 3.75 ISVTGVRR429 pKa = 11.84 GWTCRR434 pKa = 11.84 GIGGISQRR442 pKa = 11.84 KK443 pKa = 6.88 PWNPQPWSPNDD454 pKa = 3.54 EE455 pKa = 4.44 VEE457 pKa = 4.58 TNASNISLLEE467 pKa = 3.7 RR468 pKa = 11.84 RR469 pKa = 11.84 AGIEE473 pKa = 3.9 LQQLHH478 pKa = 7.19 HH479 pKa = 6.51 INKK482 pKa = 8.91 VKK484 pKa = 10.03 WSRR487 pKa = 11.84 HH488 pKa = 3.13 VRR490 pKa = 11.84 QSYY493 pKa = 10.22 KK494 pKa = 10.6 YY495 pKa = 9.98 LEE497 pKa = 4.17 LPKK500 pKa = 10.39 RR501 pKa = 11.84 LGGFGIYY508 pKa = 10.01 RR509 pKa = 11.84 FQGWLPNGKK518 pKa = 9.54 LPLAKK523 pKa = 10.09 KK524 pKa = 9.86 PLVNVEE530 pKa = 5.06 DD531 pKa = 3.48 IHH533 pKa = 6.89 PSQEE537 pKa = 3.73 LFLPLSEE544 pKa = 4.11 QQKK547 pKa = 10.69 KK548 pKa = 9.94 ILAQVEE554 pKa = 4.66 MTNKK558 pKa = 8.73 MQTDD562 pKa = 5.34 DD563 pKa = 3.86 IPGTQKK569 pKa = 10.79 LFSKK573 pKa = 10.29 EE574 pKa = 3.59 WIQKK578 pKa = 8.87 VRR580 pKa = 11.84 AKK582 pKa = 10.54 KK583 pKa = 10.31 IIWSRR588 pKa = 11.84 NQTIPIHH595 pKa = 6.05 TDD597 pKa = 2.49 HH598 pKa = 6.81 TVRR601 pKa = 11.84 IPRR604 pKa = 11.84 WDD606 pKa = 3.65 EE607 pKa = 3.9 KK608 pKa = 11.11 IKK610 pKa = 10.25 FPRR613 pKa = 11.84 YY614 pKa = 7.93 KK615 pKa = 10.25 SEE617 pKa = 4.28 YY618 pKa = 9.58 ILNNKK623 pKa = 8.89 INLTMEE629 pKa = 4.01 QVLRR633 pKa = 11.84 QYY635 pKa = 11.67 NLLKK639 pKa = 10.5 EE640 pKa = 4.18 VEE642 pKa = 4.76 RR643 pKa = 11.84 YY644 pKa = 10.28 DD645 pKa = 5.03 KK646 pKa = 10.83 DD647 pKa = 3.91 LKK649 pKa = 10.96 VPKK652 pKa = 10.42 LLDD655 pKa = 3.73 ILDD658 pKa = 3.45 KK659 pKa = 10.31 WFPVQSSKK667 pKa = 10.81 IKK669 pKa = 9.19 TYY671 pKa = 10.37 EE672 pKa = 3.73 SQGFHH677 pKa = 5.86 RR678 pKa = 11.84 TDD680 pKa = 4.5 AINLAVGEE688 pKa = 4.69 IPTEE692 pKa = 3.83 PAVKK696 pKa = 10.12 INPILINFVKK706 pKa = 10.56 LHH708 pKa = 6.44 LEE710 pKa = 3.98 RR711 pKa = 11.84 QGIRR715 pKa = 11.84 HH716 pKa = 4.24 QRR718 pKa = 11.84 GRR720 pKa = 11.84 NKK722 pKa = 9.43 IAKK725 pKa = 9.89 FIYY728 pKa = 10.03 QKK730 pKa = 9.66 TKK732 pKa = 9.28 QAEE735 pKa = 4.02 NMILQSSLQQMYY747 pKa = 10.24 RR748 pKa = 11.84 YY749 pKa = 9.9
Molecular weight: 86.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.709
IPC2_protein 8.682
IPC_protein 8.536
Toseland 9.297
ProMoST 9.092
Dawson 9.589
Bjellqvist 9.297
Wikipedia 9.75
Rodwell 9.867
Grimsley 9.663
Solomon 9.604
Lehninger 9.56
Nozaki 9.37
DTASelect 9.268
Thurlkill 9.414
EMBOSS 9.736
Sillero 9.516
Patrickios 4.8
IPC_peptide 9.604
IPC2_peptide 7.79
IPC2.peptide.svr19 7.743
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1657
749
908
828.5
93.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.699 ± 1.499
0.664 ± 0.002
5.069 ± 0.076
4.949 ± 0.777
3.742 ± 0.076
6.035 ± 0.016
1.992 ± 0.319
7.242 ± 1.232
5.975 ± 1.818
8.147 ± 0.076
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.319 ± 0.536
6.82 ± 0.475
4.647 ± 0.454
5.613 ± 0.159
4.406 ± 0.313
6.095 ± 0.442
6.518 ± 1.315
6.699 ± 0.248
1.75 ± 0.148
3.621 ± 0.46
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here