Desulfonatronum sp. SC1
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3695 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T5C7C5|A0A2T5C7C5_9DELT Tnp_DDE_dom domain-containing protein (Fragment) OS=Desulfonatronum sp. SC1 OX=2109626 GN=C6366_18355 PE=4 SV=1
CC1 pKa = 7.28 AVTVNPAPAPGEE13 pKa = 3.95 NLLTNGDD20 pKa = 4.0 FSNGLTGWLFAANGTTSSASVSNGEE45 pKa = 4.0 AVISITTAGTNAWEE59 pKa = 4.05 PQFVKK64 pKa = 10.76 DD65 pKa = 4.02 GFALVSGQQYY75 pKa = 8.3 TVSFQARR82 pKa = 11.84 AAANRR87 pKa = 11.84 NIQLFVNTGPNNYY100 pKa = 10.16 ASFFTQSVPLTTTMQTFTYY119 pKa = 9.95 TFTANANDD127 pKa = 3.6 DD128 pKa = 3.65 NARR131 pKa = 11.84 IDD133 pKa = 4.02 FNCGGNTANVIIDD146 pKa = 3.41
Molecular weight: 15.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.821
IPC2_protein 4.101
IPC_protein 3.961
Toseland 3.757
ProMoST 4.139
Dawson 3.961
Bjellqvist 4.215
Wikipedia 3.923
Rodwell 3.795
Grimsley 3.681
Solomon 3.935
Lehninger 3.897
Nozaki 4.101
DTASelect 4.317
Thurlkill 3.834
EMBOSS 3.935
Sillero 4.088
Patrickios 1.952
IPC_peptide 3.935
IPC2_peptide 4.05
IPC2.peptide.svr19 4.01
Protein with the highest isoelectric point:
>tr|A0A2T5CDL6|A0A2T5CDL6_9DELT Oxidoreductase OS=Desulfonatronum sp. SC1 OX=2109626 GN=C6366_07405 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.29 QPSNTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.25 RR14 pKa = 11.84 THH16 pKa = 6.34 GFLTRR21 pKa = 11.84 MKK23 pKa = 9.01 TKK25 pKa = 10.21 NGRR28 pKa = 11.84 AIIRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.64 GRR39 pKa = 11.84 QRR41 pKa = 11.84 LGVV44 pKa = 3.44
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3695
0
3695
1206423
27
3074
326.5
36.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.529 ± 0.047
1.184 ± 0.017
5.486 ± 0.031
6.268 ± 0.035
4.12 ± 0.026
7.884 ± 0.041
2.275 ± 0.019
5.231 ± 0.036
3.789 ± 0.039
11.191 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.649 ± 0.02
3.014 ± 0.029
5.07 ± 0.03
3.771 ± 0.023
6.799 ± 0.04
5.586 ± 0.029
5.091 ± 0.038
7.304 ± 0.036
1.311 ± 0.016
2.449 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here