Streptococcus phage Javan137
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6A050|A0A4D6A050_9CAUD Uncharacterized protein OS=Streptococcus phage Javan137 OX=2547999 GN=Javan137_0053 PE=4 SV=1
MM1 pKa = 7.05 TPKK4 pKa = 9.93 FRR6 pKa = 11.84 AYY8 pKa = 9.65 FEE10 pKa = 4.0 QYY12 pKa = 10.54 KK13 pKa = 10.66 RR14 pKa = 11.84 MIYY17 pKa = 10.29 SIGIVNQNMILVDD30 pKa = 4.07 FHH32 pKa = 7.21 DD33 pKa = 4.18 TGDD36 pKa = 3.86 TEE38 pKa = 5.13 SLFITDD44 pKa = 5.4 RR45 pKa = 11.84 IHH47 pKa = 7.32 VMQSTGLFDD56 pKa = 4.23 KK57 pKa = 11.25 NGVEE61 pKa = 4.64 IFDD64 pKa = 4.04 GDD66 pKa = 3.62 IMFYY70 pKa = 10.65 EE71 pKa = 4.15 QDD73 pKa = 3.6 CYY75 pKa = 11.28 QYY77 pKa = 11.16 TLVKK81 pKa = 10.15 YY82 pKa = 10.66 DD83 pKa = 3.48 KK84 pKa = 10.94 DD85 pKa = 3.19 KK86 pKa = 11.45 LAFVLYY92 pKa = 10.08 DD93 pKa = 3.27 GCEE96 pKa = 3.9 RR97 pKa = 11.84 LYY99 pKa = 11.2 HH100 pKa = 5.78 EE101 pKa = 4.61 LWEE104 pKa = 4.71 PGEE107 pKa = 4.34 VIGNIYY113 pKa = 9.86 EE114 pKa = 4.15 NPEE117 pKa = 3.79 LLEE120 pKa = 4.17 RR121 pKa = 11.84 LEE123 pKa = 4.34 GG124 pKa = 3.69
Molecular weight: 14.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.132
IPC2_protein 4.444
IPC_protein 4.355
Toseland 4.19
ProMoST 4.469
Dawson 4.317
Bjellqvist 4.457
Wikipedia 4.202
Rodwell 4.19
Grimsley 4.101
Solomon 4.304
Lehninger 4.266
Nozaki 4.418
DTASelect 4.596
Thurlkill 4.202
EMBOSS 4.215
Sillero 4.469
Patrickios 2.003
IPC_peptide 4.317
IPC2_peptide 4.457
IPC2.peptide.svr19 4.382
Protein with the highest isoelectric point:
>tr|A0A4D5ZZB5|A0A4D5ZZB5_9CAUD Uncharacterized protein OS=Streptococcus phage Javan137 OX=2547999 GN=Javan137_0044 PE=4 SV=1
MM1 pKa = 7.92 EE2 pKa = 6.22 DD3 pKa = 3.86 NEE5 pKa = 4.41 TCIARR10 pKa = 11.84 LKK12 pKa = 10.26 QSNIEE17 pKa = 4.01 LTQKK21 pKa = 10.34 VVDD24 pKa = 4.17 VTWKK28 pKa = 10.59 DD29 pKa = 3.23 MRR31 pKa = 11.84 RR32 pKa = 11.84 VARR35 pKa = 11.84 ARR37 pKa = 11.84 LSRR40 pKa = 11.84 RR41 pKa = 11.84 KK42 pKa = 10.01 YY43 pKa = 9.5 GVKK46 pKa = 9.84 IGG48 pKa = 3.54
Molecular weight: 5.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.251
IPC2_protein 9.443
IPC_protein 9.706
Toseland 10.57
ProMoST 10.101
Dawson 10.657
Bjellqvist 10.277
Wikipedia 10.789
Rodwell 11.038
Grimsley 10.672
Solomon 10.73
Lehninger 10.716
Nozaki 10.555
DTASelect 10.262
Thurlkill 10.555
EMBOSS 10.95
Sillero 10.57
Patrickios 10.847
IPC_peptide 10.745
IPC2_peptide 9.004
IPC2.peptide.svr19 8.562
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
10905
38
1366
198.3
22.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.841 ± 0.632
0.514 ± 0.089
6.667 ± 0.354
7.73 ± 0.342
4.099 ± 0.251
5.951 ± 0.276
1.357 ± 0.138
7.107 ± 0.323
9.574 ± 0.346
8.473 ± 0.413
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.439 ± 0.164
5.594 ± 0.254
2.641 ± 0.182
4.172 ± 0.256
4.062 ± 0.276
6.199 ± 0.354
5.869 ± 0.324
6.052 ± 0.306
1.183 ± 0.111
3.475 ± 0.307
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here