Segetibacter aerophilus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Segetibacter

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4800 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A512BA12|A0A512BA12_9BACT Oxidoreductase OS=Segetibacter aerophilus OX=670293 GN=SAE01_13010 PE=4 SV=1
MM1 pKa = 7.49SCGNYY6 pKa = 9.71KK7 pKa = 10.05EE8 pKa = 5.32VEE10 pKa = 4.15VNYY13 pKa = 10.75CPDD16 pKa = 3.19AFLFSGYY23 pKa = 10.49DD24 pKa = 3.41YY25 pKa = 10.84EE26 pKa = 6.81AYY28 pKa = 10.6VQWQFKK34 pKa = 9.38NLNGQVVRR42 pKa = 11.84GDD44 pKa = 3.99ALTSALGVLEE54 pKa = 4.66LPVDD58 pKa = 4.33GSAGLTKK65 pKa = 10.7EE66 pKa = 4.15MFQHH70 pKa = 7.01FSGFWEE76 pKa = 4.45FRR78 pKa = 11.84SWGVDD83 pKa = 3.0SLGNIDD89 pKa = 4.74PFVFCQEE96 pKa = 4.15GEE98 pKa = 4.1PFDD101 pKa = 4.32FLVFKK106 pKa = 10.23FAFIDD111 pKa = 3.95PLPEE115 pKa = 4.57PNNINILFEE124 pKa = 4.43TCTMTT129 pKa = 4.82

Molecular weight: 14.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A512BH55|A0A512BH55_9BACT Uncharacterized protein OS=Segetibacter aerophilus OX=670293 GN=SAE01_36430 PE=4 SV=1
MM1 pKa = 7.23LAARR5 pKa = 11.84IITGLFGGVIGSISLAIVADD25 pKa = 4.02LFDD28 pKa = 3.01IHH30 pKa = 7.23HH31 pKa = 7.54RR32 pKa = 11.84GRR34 pKa = 11.84VMGFVQMGFGASQVLGVHH52 pKa = 6.34IGLYY56 pKa = 9.76LANNLGWRR64 pKa = 11.84APFCLVVAMGTLIAVLIVAKK84 pKa = 10.07LKK86 pKa = 10.6PVTAHH91 pKa = 7.42LSAQHH96 pKa = 5.89SRR98 pKa = 11.84TPLRR102 pKa = 11.84YY103 pKa = 9.67LLNTFSKK110 pKa = 9.79RR111 pKa = 11.84NYY113 pKa = 9.72RR114 pKa = 11.84IRR116 pKa = 11.84FLATAFLSIGGFTMMPFSSAFAVNNLKK143 pKa = 8.35VTHH146 pKa = 6.16EE147 pKa = 3.95QLPLLFMVSGCSSLVIMPLIGRR169 pKa = 11.84IADD172 pKa = 4.89RR173 pKa = 11.84INKK176 pKa = 6.56FTLYY180 pKa = 9.73TIASALMIVVVVVYY194 pKa = 10.04TNMSVTPLAWFLPLHH209 pKa = 6.23

Molecular weight: 22.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4800

0

4800

1634465

47

3654

340.5

38.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.311 ± 0.037

0.807 ± 0.01

5.146 ± 0.024

5.919 ± 0.043

4.984 ± 0.023

6.847 ± 0.037

1.803 ± 0.016

7.065 ± 0.031

7.286 ± 0.037

9.116 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.018

5.701 ± 0.034

3.893 ± 0.02

3.667 ± 0.019

4.005 ± 0.021

6.479 ± 0.033

5.911 ± 0.036

6.661 ± 0.023

1.229 ± 0.016

3.923 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski