Acidianus filamentous virus 1 (isolate United States/Yellowstone) (AFV-1)

Taxonomy: Viruses; Adnaviria; Zilligvirae; Taleaviricota; Tokiviricetes; Ligamenvirales; Lipothrixviridae; Gammalipothrixvirus; Acidianus filamentous virus 1

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 40 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q70LC1|Y055_AFV1Y Uncharacterized protein ORF55 OS=Acidianus filamentous virus 1 (isolate United States/Yellowstone) OX=654909 GN=ORF55 PE=4 SV=1
MM1 pKa = 6.46TRR3 pKa = 11.84RR4 pKa = 11.84YY5 pKa = 9.59RR6 pKa = 11.84SNPAVIPVPFPVPLPITEE24 pKa = 4.1QTTPQIPPPSPTAIQFTCFGIPIYY48 pKa = 10.33QSPSVPKK55 pKa = 9.82SYY57 pKa = 11.15AVVNVVSHH65 pKa = 5.72NVFYY69 pKa = 10.93GCSGDD74 pKa = 3.46TCFEE78 pKa = 4.0GVEE81 pKa = 4.37GVDD84 pKa = 2.81IRR86 pKa = 11.84SFVFLPINLFAYY98 pKa = 9.57IFACEE103 pKa = 4.33APLEE107 pKa = 4.32TQEE110 pKa = 4.29TQGTQTIIVITEE122 pKa = 3.97SKK124 pKa = 10.26PLYY127 pKa = 9.3NTDD130 pKa = 3.8KK131 pKa = 10.01VTQVNQIVQGQFTDD145 pKa = 3.2KK146 pKa = 11.47SEE148 pKa = 4.13VLSINYY154 pKa = 9.96GDD156 pKa = 4.54VNVTDD161 pKa = 3.7INYY164 pKa = 10.19LSFIQTGLQIATKK177 pKa = 8.99FTLPLCDD184 pKa = 4.75RR185 pKa = 11.84ISIFKK190 pKa = 9.88TPFEE194 pKa = 3.99LAVAKK199 pKa = 9.36TLYY202 pKa = 10.81LEE204 pKa = 4.3IVGKK208 pKa = 9.66EE209 pKa = 3.92RR210 pKa = 11.84PEE212 pKa = 3.6LTAILDD218 pKa = 4.01FMQSLDD224 pKa = 3.84AEE226 pKa = 4.04ISGIIEE232 pKa = 3.64TDD234 pKa = 3.55YY235 pKa = 11.72QEE237 pKa = 4.65IEE239 pKa = 4.35PNGKK243 pKa = 8.48LTDD246 pKa = 4.18VISKK250 pKa = 9.06PIQNQLPLQILPGLILGEE268 pKa = 4.01SYY270 pKa = 10.98QFNTNNILMQTEE282 pKa = 4.11IEE284 pKa = 4.31TEE286 pKa = 3.69IGEE289 pKa = 4.26QLYY292 pKa = 10.1TDD294 pKa = 5.49GITFEE299 pKa = 4.46TEE301 pKa = 3.8LTVKK305 pKa = 10.56QSS307 pKa = 2.94

Molecular weight: 34.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q70LE0|Y166_AFV1Y Uncharacterized protein ORF166 OS=Acidianus filamentous virus 1 (isolate United States/Yellowstone) OX=654909 GN=ORF166 PE=4 SV=1
MM1 pKa = 7.49TIVNILRR8 pKa = 11.84VDD10 pKa = 3.31IDD12 pKa = 3.61QPFDD16 pKa = 3.58YY17 pKa = 10.55LDD19 pKa = 3.34KK20 pKa = 11.09QFYY23 pKa = 11.17GNLTLRR29 pKa = 11.84KK30 pKa = 8.98LLVWRR35 pKa = 11.84IFYY38 pKa = 9.94VSKK41 pKa = 10.85VFSQHH46 pKa = 5.41VEE48 pKa = 3.75SLEE51 pKa = 3.9FRR53 pKa = 11.84KK54 pKa = 10.41SLSGNIHH61 pKa = 5.71VLVTINPGIQRR72 pKa = 11.84TLVPLAQFLMGDD84 pKa = 3.77DD85 pKa = 3.56IMRR88 pKa = 11.84TFMNLKK94 pKa = 10.22RR95 pKa = 11.84KK96 pKa = 10.03GKK98 pKa = 9.79GNYY101 pKa = 9.02LFSYY105 pKa = 10.56GNTDD109 pKa = 3.08ADD111 pKa = 3.61RR112 pKa = 11.84FLAEE116 pKa = 3.69RR117 pKa = 11.84QIARR121 pKa = 11.84KK122 pKa = 9.29QKK124 pKa = 9.71ALNRR128 pKa = 11.84KK129 pKa = 8.9KK130 pKa = 10.86SKK132 pKa = 9.56TKK134 pKa = 10.2NGEE137 pKa = 3.89KK138 pKa = 10.37NGEE141 pKa = 4.23GKK143 pKa = 10.25SS144 pKa = 3.52

Molecular weight: 16.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

40

0

40

6405

48

807

160.1

18.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.856 ± 0.346

1.311 ± 0.176

5.324 ± 0.377

7.244 ± 0.734

4.403 ± 0.289

5.402 ± 0.409

1.187 ± 0.17

8.603 ± 0.356

7.447 ± 0.738

9.446 ± 0.414

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.623 ± 0.217

4.824 ± 0.216

3.497 ± 0.369

3.45 ± 0.48

4.387 ± 0.491

6.635 ± 0.467

5.605 ± 0.481

7.229 ± 0.282

0.906 ± 0.102

5.621 ± 0.387
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski