Auricularia subglabra (strain TFB-10046 / SS5) (White-rot fungus) (Auricularia delicata (strain TFB10046))

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Auriculariales; Auriculariaceae; Auricularia; Auricularia subglabra

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5290 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J0CPW2|J0CPW2_AURST Uncharacterized protein OS=Auricularia subglabra (strain TFB-10046 / SS5) OX=717982 GN=AURDEDRAFT_178730 PE=4 SV=1
MM1 pKa = 7.89ANPDD5 pKa = 3.92LPTAGDD11 pKa = 3.8ARR13 pKa = 11.84AEE15 pKa = 4.29DD16 pKa = 4.59DD17 pKa = 4.61DD18 pKa = 5.06PSFFYY23 pKa = 10.84LPPEE27 pKa = 3.78AWTTDD32 pKa = 3.15STSPSIHH39 pKa = 6.09SQSSLHH45 pKa = 5.55LTRR48 pKa = 11.84SQNAEE53 pKa = 3.68VFFVFNGTLVDD64 pKa = 4.91DD65 pKa = 5.11GAATVLSSYY74 pKa = 8.53ATSWTAGQLLYY85 pKa = 10.47TSPVLSAAQHH95 pKa = 4.94VVQFTNIAEE104 pKa = 4.48GAPFGVDD111 pKa = 2.74YY112 pKa = 11.3IMYY115 pKa = 9.93FRR117 pKa = 11.84STDD120 pKa = 4.21DD121 pKa = 5.93DD122 pKa = 6.07DD123 pKa = 7.78DD124 pKa = 7.58DD125 pKa = 7.67DD126 pKa = 7.59DD127 pKa = 7.67DD128 pKa = 7.59DD129 pKa = 7.55DD130 pKa = 7.23DD131 pKa = 7.55DD132 pKa = 5.65EE133 pKa = 4.68AQQWRR138 pKa = 11.84SGAPLADD145 pKa = 3.51RR146 pKa = 11.84RR147 pKa = 11.84NCGDD151 pKa = 3.86SPCGASGGSSGAVPTT166 pKa = 4.61

Molecular weight: 17.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J0CS47|J0CS47_AURST Uncharacterized protein OS=Auricularia subglabra (strain TFB-10046 / SS5) OX=717982 GN=AURDEDRAFT_177832 PE=4 SV=1
MM1 pKa = 7.83PSGAAPVHH9 pKa = 5.52GLARR13 pKa = 11.84RR14 pKa = 11.84PRR16 pKa = 11.84VFPFWSATCPVQNARR31 pKa = 11.84SSPRR35 pKa = 11.84VAQPLFPLQPTVPRR49 pKa = 11.84RR50 pKa = 11.84ILLPAPLVSLLVRR63 pKa = 11.84GSASVRR69 pKa = 11.84QAGSAFRR76 pKa = 11.84GAGAARR82 pKa = 11.84YY83 pKa = 8.15KK84 pKa = 10.87SPAQARR90 pKa = 11.84TPNAFSRR97 pKa = 11.84RR98 pKa = 11.84SRR100 pKa = 11.84PP101 pKa = 3.26

Molecular weight: 10.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5290

0

5290

1838300

49

3552

347.5

38.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.788 ± 0.043

1.658 ± 0.018

5.889 ± 0.027

5.391 ± 0.034

3.538 ± 0.021

6.418 ± 0.033

2.798 ± 0.017

3.931 ± 0.025

3.5 ± 0.03

9.579 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.946 ± 0.012

2.77 ± 0.017

7.126 ± 0.043

3.49 ± 0.024

7.449 ± 0.034

7.574 ± 0.034

5.629 ± 0.02

6.585 ± 0.026

1.579 ± 0.013

2.361 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski